UniProtKB - Q9Y5X5 (NPFF2_HUMAN)
Protein
Neuropeptide FF receptor 2
Gene
NPFFR2
Organism
Homo sapiens (Human)
Status
Functioni
Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
GO - Molecular functioni
- G protein-coupled receptor activity Source: ProtInc
- neuropeptide receptor activity Source: InterPro
- opioid receptor binding Source: InterPro
GO - Biological processi
- cellular response to hormone stimulus Source: GO_Central
- detection of abiotic stimulus Source: ProtInc
- G protein-coupled receptor signaling pathway Source: GO_Central
- regulation of adenylate cyclase activity Source: InterPro
- regulation of cAMP-dependent protein kinase activity Source: InterPro
- regulation of MAPK cascade Source: InterPro
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-HSA-389397 Orexin and neuropeptides FF and QRFP bind to their respective receptors R-HSA-416476 G alpha (q) signalling events |
SIGNORi | Q9Y5X5 |
Names & Taxonomyi
Protein namesi | Recommended name: Neuropeptide FF receptor 2Alternative name(s): G-protein coupled receptor 74 G-protein coupled receptor HLWAR77 Neuropeptide G-protein coupled receptor |
Gene namesi | Name:NPFFR2 Synonyms:GPR74, NPFF2, NPGPR |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000056291.17 |
HGNCi | HGNC:4525 NPFFR2 |
MIMi | 607449 gene |
neXtProti | NX_Q9Y5X5 |
Subcellular locationi
Plasma membrane
Cytoskeleton
- actin cytoskeleton Source: HPA
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: HPA
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 147 | ExtracellularSequence analysisAdd BLAST | 147 | |
Transmembranei | 148 – 168 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 169 – 184 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 185 – 205 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 206 – 221 | ExtracellularSequence analysisAdd BLAST | 16 | |
Transmembranei | 222 – 242 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 243 – 262 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 263 – 283 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 284 – 319 | ExtracellularSequence analysisAdd BLAST | 36 | |
Transmembranei | 320 – 340 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 341 – 377 | CytoplasmicSequence analysisAdd BLAST | 37 | |
Transmembranei | 378 – 398 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 399 – 413 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 414 – 434 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 435 – 522 | CytoplasmicSequence analysisAdd BLAST | 88 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 10886 |
OpenTargetsi | ENSG00000056291 |
PharmGKBi | PA28918 |
Chemistry databases
ChEMBLi | CHEMBL5952 |
GuidetoPHARMACOLOGYi | 301 |
Polymorphism and mutation databases
BioMutai | NPFFR2 |
DMDMi | 13878604 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069915 | 1 – 522 | Neuropeptide FF receptor 2Add BLAST | 522 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 110 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 122 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 133 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 220 ↔ 308 | PROSITE-ProRule annotation | ||
Glycosylationi | 300 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q9Y5X5 |
PaxDbi | Q9Y5X5 |
PeptideAtlasi | Q9Y5X5 |
PRIDEi | Q9Y5X5 |
ProteomicsDBi | 86531 86532 [Q9Y5X5-2] 86533 [Q9Y5X5-3] 86534 [Q9Y5X5-4] |
PTM databases
iPTMneti | Q9Y5X5 |
PhosphoSitePlusi | Q9Y5X5 |
Expressioni
Tissue specificityi
Isoform 1 is abundant in placenta. Relatively highly expressed in thymus, testis, and small intestine. Expressed at low levels in several tissues including spleen, prostate, brain, heart, ovary, colon, kidney, lung, liver and pancreas and not expressed in skeletal muscle and leukocytes. Isoform 2 expression is highest in placenta (but at relatively low level compared to isoform 1). Very low level of expression in numerous tissues including adipose tissue and many brain regions. Isoform 3 is expressed in brain and heart and, at lower levels, in kidney, liver, lung and pancreas.1 Publication
Gene expression databases
Bgeei | ENSG00000056291 Expressed in 59 organ(s), highest expression level in chorionic villus |
Genevisiblei | Q9Y5X5 HS |
Organism-specific databases
HPAi | HPA026825 |
Interactioni
GO - Molecular functioni
- opioid receptor binding Source: InterPro
Protein-protein interaction databases
IntActi | Q9Y5X5, 1 interactor |
STRINGi | 9606.ENSP00000307822 |
Chemistry databases
BindingDBi | Q9Y5X5 |
Structurei
3D structure databases
ProteinModelPortali | Q9Y5X5 |
SMRi | Q9Y5X5 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656 Eukaryota ENOG410XRW9 LUCA |
GeneTreei | ENSGT00940000153486 |
HOGENOMi | HOG000041285 |
HOVERGENi | HBG099624 |
InParanoidi | Q9Y5X5 |
KOi | K08375 |
OMAi | QNPHGEN |
OrthoDBi | 856254at2759 |
PhylomeDBi | Q9Y5X5 |
TreeFami | TF315303 |
Family and domain databases
InterProi | View protein in InterPro IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM IPR005395 NPFF_rcpt IPR005397 NPFF_rcpt_2 |
Pfami | View protein in Pfam PF00001 7tm_1, 1 hit |
PRINTSi | PR00237 GPCRRHODOPSN PR01570 NPFFRECEPTOR PR01572 NPFFRECEPTR2 |
SMARTi | View protein in SMART SM01381 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketNote: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q9Y5X5-1) [UniParc]FASTAAdd to basket
Also known as: long form
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MNSFFGTPAA SWCLLESDVS SAPDKEAGRE RRALSVQQRG GPAWSGSLEW
60 70 80 90 100
SRQSAGDRRR LGLSRQTAKS SWSRSRDRTC CCRRAWWILV PAADRARRER
110 120 130 140 150
FIMNEKWDTN SSENWHPIWN VNDTKHHLYS DINITYVNYY LHQPQVAAIF
160 170 180 190 200
IISYFLIFFL CMMGNTVVCF IVMRNKHMHT VTNLFILNLA ISDLLVGIFC
210 220 230 240 250
MPITLLDNII AGWPFGNTMC KISGLVQGIS VAASVFTLVA IAVDRFQCVV
260 270 280 290 300
YPFKPKLTIK TAFVIIMIIW VLAITIMSPS AVMLHVQEEK YYRVRLNSQN
310 320 330 340 350
KTSPVYWCRE DWPNQEMRKI YTTVLFANIY LAPLSLIVIM YGRIGISLFR
360 370 380 390 400
AAVPHTGRKN QEQWHVVSRK KQKIIKMLLI VALLFILSWL PLWTLMMLSD
410 420 430 440 450
YADLSPNELQ IINIYIYPFA HWLAFGNSSV NPIIYGFFNE NFRRGFQEAF
460 470 480 490 500
QLQLCQKRAK PMEAYALKAK SHVLINTSNQ LVQESTFQNP HGETLLYRKS
510 520
AEKPQQELVM EELKETTNSS EI
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 466 | A → T (PubMed:10079187).Curated | 1 | |
Sequence conflicti | 466 | A → T (PubMed:10837915).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001907 | 1 – 102 | Missing in isoform 2. 3 PublicationsAdd BLAST | 102 | |
Alternative sequenceiVSP_001908 | 1 – 99 | Missing in isoform 3. 1 PublicationAdd BLAST | 99 | |
Alternative sequenceiVSP_001909 | 100 | R → M in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_001910 | 101 – 132 | FIMNE…LYSDI → MAIWKHDVQDQWIGPGNICR SFSLYVSCNCCR in isoform 4. 1 PublicationAdd BLAST | 32 | |
Alternative sequenceiVSP_001911 | 133 – 522 | Missing in isoform 4. 1 PublicationAdd BLAST | 390 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF119815 mRNA Translation: AAD22047.1 AF257210 mRNA Translation: AAF87078.1 AF268899 mRNA Translation: AAG41398.1 AF236083 mRNA Translation: AAK58513.1 Frameshift. AF330053 mRNA Translation: AAK94197.1 AJ311393 mRNA Translation: CAC85427.1 |
CCDSi | CCDS3551.1 [Q9Y5X5-1] CCDS3552.1 [Q9Y5X5-2] CCDS47072.1 [Q9Y5X5-3] |
RefSeqi | NP_001138228.1, NM_001144756.1 [Q9Y5X5-3] NP_004876.2, NM_004885.2 [Q9Y5X5-1] NP_444264.1, NM_053036.2 [Q9Y5X5-2] |
UniGenei | Hs.99231 |
Genome annotation databases
Ensembli | ENST00000308744; ENSP00000307822; ENSG00000056291 [Q9Y5X5-1] ENST00000344413; ENSP00000340789; ENSG00000056291 [Q9Y5X5-4] ENST00000358749; ENSP00000351599; ENSG00000056291 [Q9Y5X5-2] ENST00000395999; ENSP00000379321; ENSG00000056291 [Q9Y5X5-3] |
GeneIDi | 10886 |
KEGGi | hsa:10886 |
UCSCi | uc003hgg.3 human [Q9Y5X5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF119815 mRNA Translation: AAD22047.1 AF257210 mRNA Translation: AAF87078.1 AF268899 mRNA Translation: AAG41398.1 AF236083 mRNA Translation: AAK58513.1 Frameshift. AF330053 mRNA Translation: AAK94197.1 AJ311393 mRNA Translation: CAC85427.1 |
CCDSi | CCDS3551.1 [Q9Y5X5-1] CCDS3552.1 [Q9Y5X5-2] CCDS47072.1 [Q9Y5X5-3] |
RefSeqi | NP_001138228.1, NM_001144756.1 [Q9Y5X5-3] NP_004876.2, NM_004885.2 [Q9Y5X5-1] NP_444264.1, NM_053036.2 [Q9Y5X5-2] |
UniGenei | Hs.99231 |
3D structure databases
ProteinModelPortali | Q9Y5X5 |
SMRi | Q9Y5X5 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
IntActi | Q9Y5X5, 1 interactor |
STRINGi | 9606.ENSP00000307822 |
Chemistry databases
BindingDBi | Q9Y5X5 |
ChEMBLi | CHEMBL5952 |
GuidetoPHARMACOLOGYi | 301 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
iPTMneti | Q9Y5X5 |
PhosphoSitePlusi | Q9Y5X5 |
Polymorphism and mutation databases
BioMutai | NPFFR2 |
DMDMi | 13878604 |
Proteomic databases
EPDi | Q9Y5X5 |
PaxDbi | Q9Y5X5 |
PeptideAtlasi | Q9Y5X5 |
PRIDEi | Q9Y5X5 |
ProteomicsDBi | 86531 86532 [Q9Y5X5-2] 86533 [Q9Y5X5-3] 86534 [Q9Y5X5-4] |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENST00000308744; ENSP00000307822; ENSG00000056291 [Q9Y5X5-1] ENST00000344413; ENSP00000340789; ENSG00000056291 [Q9Y5X5-4] ENST00000358749; ENSP00000351599; ENSG00000056291 [Q9Y5X5-2] ENST00000395999; ENSP00000379321; ENSG00000056291 [Q9Y5X5-3] |
GeneIDi | 10886 |
KEGGi | hsa:10886 |
UCSCi | uc003hgg.3 human [Q9Y5X5-1] |
Organism-specific databases
CTDi | 10886 |
DisGeNETi | 10886 |
EuPathDBi | HostDB:ENSG00000056291.17 |
GeneCardsi | NPFFR2 |
HGNCi | HGNC:4525 NPFFR2 |
HPAi | HPA026825 |
MIMi | 607449 gene |
neXtProti | NX_Q9Y5X5 |
OpenTargetsi | ENSG00000056291 |
PharmGKBi | PA28918 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3656 Eukaryota ENOG410XRW9 LUCA |
GeneTreei | ENSGT00940000153486 |
HOGENOMi | HOG000041285 |
HOVERGENi | HBG099624 |
InParanoidi | Q9Y5X5 |
KOi | K08375 |
OMAi | QNPHGEN |
OrthoDBi | 856254at2759 |
PhylomeDBi | Q9Y5X5 |
TreeFami | TF315303 |
Enzyme and pathway databases
Reactomei | R-HSA-389397 Orexin and neuropeptides FF and QRFP bind to their respective receptors R-HSA-416476 G alpha (q) signalling events |
SIGNORi | Q9Y5X5 |
Miscellaneous databases
GenomeRNAii | 10886 |
PROi | PR:Q9Y5X5 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000056291 Expressed in 59 organ(s), highest expression level in chorionic villus |
Genevisiblei | Q9Y5X5 HS |
Family and domain databases
InterProi | View protein in InterPro IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM IPR005395 NPFF_rcpt IPR005397 NPFF_rcpt_2 |
Pfami | View protein in Pfam PF00001 7tm_1, 1 hit |
PRINTSi | PR00237 GPCRRHODOPSN PR01570 NPFFRECEPTOR PR01572 NPFFRECEPTR2 |
SMARTi | View protein in SMART SM01381 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | NPFF2_HUMAN | |
Accessioni | Q9Y5X5Primary (citable) accession number: Q9Y5X5 Secondary accession number(s): Q96RV1, Q9NR49 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | April 27, 2001 | |
Last modified: | February 13, 2019 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries