Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - Q9Y5X5 (NPFF2_HUMAN)

Protein

Neuropeptide FF receptor 2

Gene

NPFFR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

  • G protein-coupled receptor activity Source: ProtInc
  • neuropeptide receptor activity Source: InterPro
  • opioid receptor binding Source: InterPro
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-389397 Orexin and neuropeptides FF and QRFP bind to their respective receptors
R-HSA-416476 G alpha (q) signalling events
SIGNORiQ9Y5X5

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropeptide FF receptor 2
Alternative name(s):
G-protein coupled receptor 74
G-protein coupled receptor HLWAR77
Neuropeptide G-protein coupled receptor
Gene namesi
Name:NPFFR2
Synonyms:GPR74, NPFF2, NPGPR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000056291.17
HGNCiHGNC:4525 NPFFR2
MIMi607449 gene
neXtProtiNX_Q9Y5X5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 147ExtracellularSequence analysisAdd BLAST147
Transmembranei148 – 168Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini169 – 184CytoplasmicSequence analysisAdd BLAST16
Transmembranei185 – 205Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini206 – 221ExtracellularSequence analysisAdd BLAST16
Transmembranei222 – 242Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini243 – 262CytoplasmicSequence analysisAdd BLAST20
Transmembranei263 – 283Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini284 – 319ExtracellularSequence analysisAdd BLAST36
Transmembranei320 – 340Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini341 – 377CytoplasmicSequence analysisAdd BLAST37
Transmembranei378 – 398Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini399 – 413ExtracellularSequence analysisAdd BLAST15
Transmembranei414 – 434Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini435 – 522CytoplasmicSequence analysisAdd BLAST88

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10886
OpenTargetsiENSG00000056291
PharmGKBiPA28918

Chemistry databases

ChEMBLiCHEMBL5952
GuidetoPHARMACOLOGYi301

Polymorphism and mutation databases

BioMutaiNPFFR2
DMDMi13878604

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699151 – 522Neuropeptide FF receptor 2Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi133N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi220 ↔ 308PROSITE-ProRule annotation
Glycosylationi300N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ9Y5X5
PaxDbiQ9Y5X5
PeptideAtlasiQ9Y5X5
PRIDEiQ9Y5X5
ProteomicsDBi86531
86532 [Q9Y5X5-2]
86533 [Q9Y5X5-3]
86534 [Q9Y5X5-4]

PTM databases

iPTMnetiQ9Y5X5
PhosphoSitePlusiQ9Y5X5

Expressioni

Tissue specificityi

Isoform 1 is abundant in placenta. Relatively highly expressed in thymus, testis, and small intestine. Expressed at low levels in several tissues including spleen, prostate, brain, heart, ovary, colon, kidney, lung, liver and pancreas and not expressed in skeletal muscle and leukocytes. Isoform 2 expression is highest in placenta (but at relatively low level compared to isoform 1). Very low level of expression in numerous tissues including adipose tissue and many brain regions. Isoform 3 is expressed in brain and heart and, at lower levels, in kidney, liver, lung and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000056291 Expressed in 59 organ(s), highest expression level in chorionic villus
CleanExiHS_NPFFR2
ExpressionAtlasiQ9Y5X5 baseline and differential
GenevisibleiQ9Y5X5 HS

Organism-specific databases

HPAiHPA026825

Interactioni

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

IntActiQ9Y5X5, 1 interactor
STRINGi9606.ENSP00000307822

Chemistry databases

BindingDBiQ9Y5X5

Structurei

3D structure databases

ProteinModelPortaliQ9Y5X5
SMRiQ9Y5X5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00930000150833
HOGENOMiHOG000041285
HOVERGENiHBG099624
InParanoidiQ9Y5X5
KOiK08375
OMAiQNPHGEN
OrthoDBiEOG091G098F
PhylomeDBiQ9Y5X5
TreeFamiTF315303

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR005395 NPFF_rcpt
IPR005397 NPFF_rcpt_2
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR01570 NPFFRECEPTOR
PR01572 NPFFRECEPTR2
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q9Y5X5-1) [UniParc]FASTAAdd to basket
Also known as: long form

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNSFFGTPAA SWCLLESDVS SAPDKEAGRE RRALSVQQRG GPAWSGSLEW 
        60         70         80         90        100
SRQSAGDRRR LGLSRQTAKS SWSRSRDRTC CCRRAWWILV PAADRARRER 
       110        120        130        140        150
FIMNEKWDTN SSENWHPIWN VNDTKHHLYS DINITYVNYY LHQPQVAAIF 
       160        170        180        190        200
IISYFLIFFL CMMGNTVVCF IVMRNKHMHT VTNLFILNLA ISDLLVGIFC 
       210        220        230        240        250
MPITLLDNII AGWPFGNTMC KISGLVQGIS VAASVFTLVA IAVDRFQCVV 
       260        270        280        290        300
YPFKPKLTIK TAFVIIMIIW VLAITIMSPS AVMLHVQEEK YYRVRLNSQN 
       310        320        330        340        350
KTSPVYWCRE DWPNQEMRKI YTTVLFANIY LAPLSLIVIM YGRIGISLFR 
       360        370        380        390        400
AAVPHTGRKN QEQWHVVSRK KQKIIKMLLI VALLFILSWL PLWTLMMLSD 
       410        420        430        440        450
YADLSPNELQ IINIYIYPFA HWLAFGNSSV NPIIYGFFNE NFRRGFQEAF 
       460        470        480        490        500
QLQLCQKRAK PMEAYALKAK SHVLINTSNQ LVQESTFQNP HGETLLYRKS 
       510        520 
AEKPQQELVM EELKETTNSS EI
Length:522
Mass (Da):60,270
Last modified:April 27, 2001 - v2
Checksum:i40CB9FCD42F77041
GO
Isoform 2 (identifier: Q9Y5X5-2) [UniParc]FASTAAdd to basket
Also known as: short form

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.

Show »
Length:420
Mass (Da):48,686
Checksum:i7A47C4CEEC1DBE07
GO
Isoform 3 (identifier: Q9Y5X5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-99: Missing.
     100-100: R → M

Show »
Length:423
Mass (Da):49,078
Checksum:i767946C4564A84F4
GO
Isoform 4 (identifier: Q9Y5X5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-132: FIMNEKWDTNSSENWHPIWNVNDTKHHLYSDI → MAIWKHDVQDQWIGPGNICRSFSLYVSCNCCR
     133-522: Missing.

Show »
Length:132
Mass (Da):15,055
Checksum:i0C561F6F086FC888
GO

Sequence cautioni

The sequence AAK58513 differs from that shown. Reason: Frameshift at position 503.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti466A → T (PubMed:10079187).Curated1
Sequence conflicti466A → T (PubMed:10837915).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0019071 – 102Missing in isoform 2. 3 PublicationsAdd BLAST102
Alternative sequenceiVSP_0019081 – 99Missing in isoform 3. 1 PublicationAdd BLAST99
Alternative sequenceiVSP_001909100R → M in isoform 3. 1 Publication1
Alternative sequenceiVSP_001910101 – 132FIMNE…LYSDI → MAIWKHDVQDQWIGPGNICR SFSLYVSCNCCR in isoform 4. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_001911133 – 522Missing in isoform 4. 1 PublicationAdd BLAST390

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF119815 mRNA Translation: AAD22047.1
AF257210 mRNA Translation: AAF87078.1
AF268899 mRNA Translation: AAG41398.1
AF236083 mRNA Translation: AAK58513.1 Frameshift.
AF330053 mRNA Translation: AAK94197.1
AJ311393 mRNA Translation: CAC85427.1
CCDSiCCDS3551.1 [Q9Y5X5-1]
CCDS3552.1 [Q9Y5X5-2]
CCDS47072.1 [Q9Y5X5-3]
RefSeqiNP_001138228.1, NM_001144756.1 [Q9Y5X5-3]
NP_004876.2, NM_004885.2 [Q9Y5X5-1]
NP_444264.1, NM_053036.2 [Q9Y5X5-2]
UniGeneiHs.99231

Genome annotation databases

EnsembliENST00000308744; ENSP00000307822; ENSG00000056291 [Q9Y5X5-1]
ENST00000344413; ENSP00000340789; ENSG00000056291 [Q9Y5X5-4]
ENST00000358749; ENSP00000351599; ENSG00000056291 [Q9Y5X5-2]
ENST00000395999; ENSP00000379321; ENSG00000056291 [Q9Y5X5-3]
GeneIDi10886
KEGGihsa:10886
UCSCiuc003hgg.3 human [Q9Y5X5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF119815 mRNA Translation: AAD22047.1
AF257210 mRNA Translation: AAF87078.1
AF268899 mRNA Translation: AAG41398.1
AF236083 mRNA Translation: AAK58513.1 Frameshift.
AF330053 mRNA Translation: AAK94197.1
AJ311393 mRNA Translation: CAC85427.1
CCDSiCCDS3551.1 [Q9Y5X5-1]
CCDS3552.1 [Q9Y5X5-2]
CCDS47072.1 [Q9Y5X5-3]
RefSeqiNP_001138228.1, NM_001144756.1 [Q9Y5X5-3]
NP_004876.2, NM_004885.2 [Q9Y5X5-1]
NP_444264.1, NM_053036.2 [Q9Y5X5-2]
UniGeneiHs.99231

3D structure databases

ProteinModelPortaliQ9Y5X5
SMRiQ9Y5X5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Y5X5, 1 interactor
STRINGi9606.ENSP00000307822

Chemistry databases

BindingDBiQ9Y5X5
ChEMBLiCHEMBL5952
GuidetoPHARMACOLOGYi301

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ9Y5X5
PhosphoSitePlusiQ9Y5X5

Polymorphism and mutation databases

BioMutaiNPFFR2
DMDMi13878604

Proteomic databases

EPDiQ9Y5X5
PaxDbiQ9Y5X5
PeptideAtlasiQ9Y5X5
PRIDEiQ9Y5X5
ProteomicsDBi86531
86532 [Q9Y5X5-2]
86533 [Q9Y5X5-3]
86534 [Q9Y5X5-4]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000308744; ENSP00000307822; ENSG00000056291 [Q9Y5X5-1]
ENST00000344413; ENSP00000340789; ENSG00000056291 [Q9Y5X5-4]
ENST00000358749; ENSP00000351599; ENSG00000056291 [Q9Y5X5-2]
ENST00000395999; ENSP00000379321; ENSG00000056291 [Q9Y5X5-3]
GeneIDi10886
KEGGihsa:10886
UCSCiuc003hgg.3 human [Q9Y5X5-1]

Organism-specific databases

CTDi10886
DisGeNETi10886
EuPathDBiHostDB:ENSG00000056291.17
GeneCardsiNPFFR2
HGNCiHGNC:4525 NPFFR2
HPAiHPA026825
MIMi607449 gene
neXtProtiNX_Q9Y5X5
OpenTargetsiENSG00000056291
PharmGKBiPA28918
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00930000150833
HOGENOMiHOG000041285
HOVERGENiHBG099624
InParanoidiQ9Y5X5
KOiK08375
OMAiQNPHGEN
OrthoDBiEOG091G098F
PhylomeDBiQ9Y5X5
TreeFamiTF315303

Enzyme and pathway databases

ReactomeiR-HSA-389397 Orexin and neuropeptides FF and QRFP bind to their respective receptors
R-HSA-416476 G alpha (q) signalling events
SIGNORiQ9Y5X5

Miscellaneous databases

GenomeRNAii10886
PROiPR:Q9Y5X5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000056291 Expressed in 59 organ(s), highest expression level in chorionic villus
CleanExiHS_NPFFR2
ExpressionAtlasiQ9Y5X5 baseline and differential
GenevisibleiQ9Y5X5 HS

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR005395 NPFF_rcpt
IPR005397 NPFF_rcpt_2
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR01570 NPFFRECEPTOR
PR01572 NPFFRECEPTR2
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNPFF2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5X5
Secondary accession number(s): Q96RV1, Q9NR49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: November 7, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again