UniProtKB - Q9Y5S8 (NOX1_HUMAN)
NADPH oxidase 1
NOX1
Functioni
NOH-1S is a voltage-gated proton channel that mediates the H+ currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H+ ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
Cofactori
Protein has several cofactor binding sites:Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 101 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 115 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 209 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 221 | Iron (heme axial ligand)Curated | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 338 – 344 | FADSequence analysis | 7 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- NADP binding Source: BHF-UCL
- small GTPase binding Source: BHF-UCL
- superoxide-generating NAD(P)H oxidase activity Source: BHF-UCL
- voltage-gated ion channel activity Source: UniProtKB-KW
GO - Biological processi
- angiogenesis Source: BHF-UCL
- cell migration Source: BHF-UCL
- cellular response to hyperoxia Source: Ensembl
- cellular stress response to acidic pH Source: BHF-UCL
- defense response Source: GO_Central
- extracellular matrix organization Source: Ensembl
- hydrogen peroxide metabolic process Source: BHF-UCL
- inflammatory response Source: BHF-UCL
- intracellular pH elevation Source: BHF-UCL
- NADP metabolic process Source: BHF-UCL
- oxygen metabolic process Source: Ensembl
- positive regulation of cell population proliferation Source: BHF-UCL
- positive regulation of integrin biosynthetic process Source: BHF-UCL
- positive regulation of JNK cascade Source: Ensembl
- positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: Ensembl
- positive regulation of smooth muscle cell proliferation Source: BHF-UCL
- positive regulation of vascular endothelial growth factor production Source: BHF-UCL
- regulation of blood pressure Source: BHF-UCL
- regulation of ion transmembrane transport Source: UniProtKB-KW
- regulation of systemic arterial blood pressure by renin-angiotensin Source: Ensembl
- respiratory burst Source: BHF-UCL
- signal transduction Source: BHF-UCL
- superoxide anion generation Source: BHF-UCL
Keywordsi
Molecular function | Ion channel, Oxidoreductase, Voltage-gated channel |
Biological process | Electron transport, Ion transport, Transport |
Ligand | FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
PathwayCommonsi | Q9Y5S8 |
Reactomei | R-HSA-5668599, RHO GTPases Activate NADPH Oxidases R-HSA-9013149, RAC1 GTPase cycle R-HSA-9013423, RAC3 GTPase cycle R-HSA-9673324, WNT5:FZD7-mediated leishmania damping |
SignaLinki | Q9Y5S8 |
SIGNORi | Q9Y5S8 |
Protein family/group databases
PeroxiBasei | 5410, HsNOx01 |
TCDBi | 5.B.1.1.3, the phagocyte (gp91(phox)) nadph oxidase family |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH oxidase 1 (EC:1.-.-.-)Short name: NOX-1 Alternative name(s): Mitogenic oxidase 1 Short name: MOX-1 NADH/NADPH mitogenic oxidase subunit P65-MOX NOH-1 |
Gene namesi | Name:NOX1 Synonyms:MOX1, NOH1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7889, NOX1 |
MIMi | 300225, gene |
neXtProti | NX_Q9Y5S8 |
VEuPathDBi | HostDB:ENSG00000007952 |
Subcellular locationi
Plasma membrane
- invadopodium membrane 1 Publication; Multi-pass membrane protein 1 Publication
- Cell membrane 1 Publication
Endosome
- early endosome Source: BHF-UCL
Plasma Membrane
- NADPH oxidase complex Source: BHF-UCL
- plasma membrane Source: GO_Central
Other locations
- cell junction Source: UniProtKB-KW
- cell projection Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 10 – 30 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 31 – 44 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 45 – 72 | HelicalSequence analysisAdd BLAST | 28 | |
Topological domaini | 73 – 102 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 103 – 123 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 124 – 168 | ExtracellularSequence analysisAdd BLAST | 45 | |
Transmembranei | 169 – 189 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 190 – 206 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 207 – 227 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 228 – 396 | ExtracellularSequence analysisAdd BLAST | 169 | |
Transmembranei | 397 – 417 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 418 – 564 | CytoplasmicSequence analysisAdd BLAST | 147 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, MembranePathology & Biotechi
Involvement in diseasei
Organism-specific databases
DisGeNETi | 27035 |
OpenTargetsi | ENSG00000007952 |
PharmGKBi | PA31690 |
Miscellaneous databases
Pharosi | Q9Y5S8, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1287628 |
DrugBanki | DB09140, Oxygen |
GuidetoPHARMACOLOGYi | 3001 |
Genetic variation databases
BioMutai | NOX1 |
DMDMi | 8134597 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210148 | 1 – 564 | NADPH oxidase 1Add BLAST | 564 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 162 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 236 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
jPOSTi | Q9Y5S8 |
MassIVEi | Q9Y5S8 |
PaxDbi | Q9Y5S8 |
PeptideAtlasi | Q9Y5S8 |
PRIDEi | Q9Y5S8 |
ProteomicsDBi | 86491 [Q9Y5S8-1] 86492 [Q9Y5S8-2] 86493 [Q9Y5S8-3] |
PTM databases
GlyGeni | Q9Y5S8, 2 sites |
iPTMneti | Q9Y5S8 |
PhosphoSitePlusi | Q9Y5S8 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000007952, Expressed in colonic mucosa and 141 other tissues |
ExpressionAtlasi | Q9Y5S8, baseline and differential |
Genevisiblei | Q9Y5S8, HS |
Organism-specific databases
HPAi | ENSG00000007952, Tissue enriched (intestine) |
Interactioni
Subunit structurei
NOX1, NOXA1, NOXO1, RAC1 and CYBA forms a functional multimeric complex supporting reactive oxygen species (ROS) production.
Interacts with NOXA1 and NOXO1.
2 PublicationsBinary interactionsi
Q9Y5S8
With | #Exp. | IntAct |
---|---|---|
TBXA2R [Q0VAB0] | 3 | EBI-15795558,EBI-18271435 |
GO - Molecular functioni
- small GTPase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 117966, 11 interactors |
DIPi | DIP-60457N |
IntActi | Q9Y5S8, 5 interactors |
STRINGi | 9606.ENSP00000362057 |
Chemistry databases
BindingDBi | Q9Y5S8 |
Miscellaneous databases
RNActi | Q9Y5S8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 54 – 283 | Ferric oxidoreductaseAdd BLAST | 230 | |
Domaini | 284 – 391 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 108 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 397 – 536 | Interaction with NOXO11 PublicationAdd BLAST | 140 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0039, Eukaryota |
GeneTreei | ENSGT00940000161632 |
HOGENOMi | CLU_005646_3_1_1 |
InParanoidi | Q9Y5S8 |
OMAi | HRTYISK |
OrthoDBi | 936110at2759 |
PhylomeDBi | Q9Y5S8 |
TreeFami | TF105354 |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR029650, NOX1 IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR11972:SF71, PTHR11972:SF71, 1 hit |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGNWVVNHWF SVLFLVVWLG LNVFLFVDAF LKYEKADKYY YTRKILGSTL
60 70 80 90 100
ACARASALCL NFNSTLILLP VCRNLLSFLR GTCSFCSRTL RKQLDHNLTF
110 120 130 140 150
HKLVAYMICL HTAIHIIAHL FNFDCYSRSR QATDGSLASI LSSLSHDEKK
160 170 180 190 200
GGSWLNPIQS RNTTVEYVTF TSIAGLTGVI MTIALILMVT SATEFIRRSY
210 220 230 240 250
FEVFWYTHHL FIFYILGLGI HGIGGIVRGQ TEESMNESHP RKCAESFEMW
260 270 280 290 300
DDRDSHCRRP KFEGHPPESW KWILAPVILY ICERILRFYR SQQKVVITKV
310 320 330 340 350
VMHPSKVLEL QMNKRGFSME VGQYIFVNCP SISLLEWHPF TLTSAPEEDF
360 370 380 390 400
FSIHIRAAGD WTENLIRAFE QQYSPIPRIE VDGPFGTASE DVFQYEVAVL
410 420 430 440 450
VGAGIGVTPF ASILKSIWYK FQCADHNLKT KKIYFYWICR ETGAFSWFNN
460 470 480 490 500
LLTSLEQEME ELGKVGFLNY RLFLTGWDSN IVGHAALNFD KATDIVTGLK
510 520 530 540 550
QKTSFGRPMW DNEFSTIATS HPKSVVGVFL CGPRTLAKSL RKCCHRYSSL
560
DPRKVQFYFN KENF
The sequence of this isoform differs from the canonical sequence as follows:
159-190: QSRNTTVEYVTFTSIAGLTGVIMTIALILMVT → HPHITPTVYMFTVTFDMVLSSVNSNLLFLLIK
191-564: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
433-481: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA6NGA6 | A6NGA6_HUMAN | NADPH oxidase 1 | NOX1 | 527 | Annotation score: | ||
Q5H9D4 | Q5H9D4_HUMAN | NADPH oxidase 1 | NOX1 | 192 | Annotation score: | ||
H0Y581 | H0Y581_HUMAN | NADPH oxidase 1 | NOX1 | 175 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 173 | I → V in AAD38133 (PubMed:10615049).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049101 | 315 | R → H. Corresponds to variant dbSNP:rs2071756Ensembl. | 1 | |
Natural variantiVAR_075548 | 330 | P → S Found in a patient with very early onset inflammatory bowel disease; unknown pathological significance; no effect on subcellular location; significantly reduced basal and phorbol ester-stimulated ROS generation, which may decrease resistance to infection by enteric pathogens, such as Campylobacter jejuni. 1 Publication | 1 | |
Natural variantiVAR_061176 | 360 | D → N Found in a patient with very early onset inflammatory bowel disease; unknown pathological significance; no effect on subcellular location; significantly reduced basal and phorbol ester-stimulated ROS generation, which may decrease resistance to infection by enteric pathogens, such as Campylobacter jejuni. 1 PublicationCorresponds to variant dbSNP:rs34688635Ensembl. | 1 | |
Natural variantiVAR_049102 | 378 | R → K. Corresponds to variant dbSNP:rs35404864EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001577 | 159 – 190 | QSRNT…ILMVT → HPHITPTVYMFTVTFDMVLS SVNSNLLFLLIK in isoform NOH-1S. 1 PublicationAdd BLAST | 32 | |
Alternative sequenceiVSP_001578 | 191 – 564 | Missing in isoform NOH-1S. 1 PublicationAdd BLAST | 374 | |
Alternative sequenceiVSP_001579 | 433 – 481 | Missing in isoform NOH-1LV. 1 PublicationAdd BLAST | 49 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF127763 mRNA Translation: AAD38133.1 AF166326 mRNA Translation: AAF23232.1 AF166327 mRNA Translation: AAF23233.1 AF166328 mRNA Translation: AAF23234.1 DQ314883 Genomic DNA Translation: ABC40742.1 AK292201 mRNA Translation: BAF84890.1 Z83819 Genomic DNA No translation available. CH471115 Genomic DNA Translation: EAX02820.1 BC075014 mRNA Translation: AAH75014.1 BC075015 mRNA Translation: AAH75015.1 |
CCDSi | CCDS14474.1 [Q9Y5S8-1] CCDS14475.1 [Q9Y5S8-3] |
RefSeqi | NP_001258744.1, NM_001271815.1 NP_008983.2, NM_007052.4 [Q9Y5S8-1] NP_039249.1, NM_013955.2 [Q9Y5S8-3] |
Genome annotation databases
Ensembli | ENST00000217885.5; ENSP00000217885.5; ENSG00000007952.18 [Q9Y5S8-3] ENST00000372966.8; ENSP00000362057.3; ENSG00000007952.18 |
GeneIDi | 27035 |
KEGGi | hsa:27035 |
MANE-Selecti | ENST00000372966.8; ENSP00000362057.3; NM_007052.5; NP_008983.2 |
UCSCi | uc004egj.3, human [Q9Y5S8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF127763 mRNA Translation: AAD38133.1 AF166326 mRNA Translation: AAF23232.1 AF166327 mRNA Translation: AAF23233.1 AF166328 mRNA Translation: AAF23234.1 DQ314883 Genomic DNA Translation: ABC40742.1 AK292201 mRNA Translation: BAF84890.1 Z83819 Genomic DNA No translation available. CH471115 Genomic DNA Translation: EAX02820.1 BC075014 mRNA Translation: AAH75014.1 BC075015 mRNA Translation: AAH75015.1 |
CCDSi | CCDS14474.1 [Q9Y5S8-1] CCDS14475.1 [Q9Y5S8-3] |
RefSeqi | NP_001258744.1, NM_001271815.1 NP_008983.2, NM_007052.4 [Q9Y5S8-1] NP_039249.1, NM_013955.2 [Q9Y5S8-3] |
3D structure databases
AlphaFoldDBi | Q9Y5S8 |
SMRi | Q9Y5S8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 117966, 11 interactors |
DIPi | DIP-60457N |
IntActi | Q9Y5S8, 5 interactors |
STRINGi | 9606.ENSP00000362057 |
Chemistry databases
BindingDBi | Q9Y5S8 |
ChEMBLi | CHEMBL1287628 |
DrugBanki | DB09140, Oxygen |
GuidetoPHARMACOLOGYi | 3001 |
Protein family/group databases
PeroxiBasei | 5410, HsNOx01 |
TCDBi | 5.B.1.1.3, the phagocyte (gp91(phox)) nadph oxidase family |
PTM databases
GlyGeni | Q9Y5S8, 2 sites |
iPTMneti | Q9Y5S8 |
PhosphoSitePlusi | Q9Y5S8 |
Genetic variation databases
BioMutai | NOX1 |
DMDMi | 8134597 |
Proteomic databases
jPOSTi | Q9Y5S8 |
MassIVEi | Q9Y5S8 |
PaxDbi | Q9Y5S8 |
PeptideAtlasi | Q9Y5S8 |
PRIDEi | Q9Y5S8 |
ProteomicsDBi | 86491 [Q9Y5S8-1] 86492 [Q9Y5S8-2] 86493 [Q9Y5S8-3] |
Protocols and materials databases
Antibodypediai | 28538, 370 antibodies from 34 providers |
DNASUi | 27035 |
Genome annotation databases
Ensembli | ENST00000217885.5; ENSP00000217885.5; ENSG00000007952.18 [Q9Y5S8-3] ENST00000372966.8; ENSP00000362057.3; ENSG00000007952.18 |
GeneIDi | 27035 |
KEGGi | hsa:27035 |
MANE-Selecti | ENST00000372966.8; ENSP00000362057.3; NM_007052.5; NP_008983.2 |
UCSCi | uc004egj.3, human [Q9Y5S8-1] |
Organism-specific databases
CTDi | 27035 |
DisGeNETi | 27035 |
GeneCardsi | NOX1 |
HGNCi | HGNC:7889, NOX1 |
HPAi | ENSG00000007952, Tissue enriched (intestine) |
MIMi | 300225, gene |
neXtProti | NX_Q9Y5S8 |
OpenTargetsi | ENSG00000007952 |
PharmGKBi | PA31690 |
VEuPathDBi | HostDB:ENSG00000007952 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0039, Eukaryota |
GeneTreei | ENSGT00940000161632 |
HOGENOMi | CLU_005646_3_1_1 |
InParanoidi | Q9Y5S8 |
OMAi | HRTYISK |
OrthoDBi | 936110at2759 |
PhylomeDBi | Q9Y5S8 |
TreeFami | TF105354 |
Enzyme and pathway databases
PathwayCommonsi | Q9Y5S8 |
Reactomei | R-HSA-5668599, RHO GTPases Activate NADPH Oxidases R-HSA-9013149, RAC1 GTPase cycle R-HSA-9013423, RAC3 GTPase cycle R-HSA-9673324, WNT5:FZD7-mediated leishmania damping |
SignaLinki | Q9Y5S8 |
SIGNORi | Q9Y5S8 |
Miscellaneous databases
BioGRID-ORCSi | 27035, 8 hits in 694 CRISPR screens |
ChiTaRSi | NOX1, human |
GeneWikii | NOX1 |
GenomeRNAii | 27035 |
Pharosi | Q9Y5S8, Tchem |
PROi | PR:Q9Y5S8 |
RNActi | Q9Y5S8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000007952, Expressed in colonic mucosa and 141 other tissues |
ExpressionAtlasi | Q9Y5S8, baseline and differential |
Genevisiblei | Q9Y5S8, HS |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR029650, NOX1 IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR11972:SF71, PTHR11972:SF71, 1 hit |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NOX1_HUMAN | |
Accessioni | Q9Y5S8Primary (citable) accession number: Q9Y5S8 Secondary accession number(s): A8K836, O95691, Q2PP02 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot