UniProtKB - Q9Y5K3 (PCY1B_HUMAN)
Protein
Choline-phosphate cytidylyltransferase B
Gene
PCYT1B
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.2 Publications
Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.1 Publication
Catalytic activityi
: phosphatidylcholine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.2 PublicationsProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Choline-phosphate cytidylyltransferase B (PCYT1B), Choline-phosphate cytidylyltransferase A (PCYT1A)
- Cholinephosphotransferase 1 (CHPT1), Choline/ethanolaminephosphotransferase 1 (CEPT1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 122 | CTPBy similarity | 1 | |
Binding sitei | 122 | SubstrateBy similarity | 1 | |
Binding sitei | 151 | SubstrateBy similarity | 1 | |
Binding sitei | 173 | CTPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 84 – 92 | CTPBy similarity | 9 | |
Nucleotide bindingi | 168 – 169 | CTPBy similarity | 2 | |
Nucleotide bindingi | 196 – 200 | CTPBy similarity | 5 |
GO - Molecular functioni
- choline-phosphate cytidylyltransferase activity Source: UniProtKB
- identical protein binding Source: IntAct
- phosphatidylcholine binding Source: GO_Central
GO - Biological processi
- CDP-choline pathway Source: UniProtKB
- ovarian follicle development Source: Ensembl
- phosphatidylcholine biosynthetic process Source: UniProtKB
- spermatogenesis Source: Ensembl
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Enzyme and pathway databases
PathwayCommonsi | Q9Y5K3 |
Reactomei | R-HSA-1483191, Synthesis of PC |
UniPathwayi | UPA00753;UER00739 |
Chemistry databases
SwissLipidsi | SLP:000001754 |
Names & Taxonomyi
Protein namesi | Recommended name: Choline-phosphate cytidylyltransferase B (EC:2.7.7.152 Publications)Alternative name(s): CCT-beta CTP:phosphocholine cytidylyltransferase B Short name: CCT B Short name: CT B Phosphorylcholine transferase B |
Gene namesi | Name:PCYT1B Synonyms:CCTB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8755, PCYT1B |
MIMi | 300948, gene |
neXtProti | NX_Q9Y5K3 |
VEuPathDBi | HostDB:ENSG00000102230.13 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: Reactome
Other locations
- cytoplasm Source: ProtInc
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulumPathology & Biotechi
Organism-specific databases
DisGeNETi | 9468 |
OpenTargetsi | ENSG00000102230 |
PharmGKBi | PA33100 |
Miscellaneous databases
Pharosi | Q9Y5K3, Tbio |
Chemistry databases
DrugBanki | DB00122, Choline DB14006, Choline salicylate |
Genetic variation databases
BioMutai | PCYT1B |
DMDMi | 12643330 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208456 | 1 – 369 | Choline-phosphate cytidylyltransferase BAdd BLAST | 369 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 315 | PhosphoserineCombined sources | 1 | |
Modified residuei | 319 | PhosphoserineCombined sources | 1 | |
Modified residuei | 322 | PhosphoserineBy similarity | 1 | |
Modified residuei | 323 | PhosphoserineBy similarity | 1 | |
Modified residuei | 329 | PhosphoserineBy similarity | 1 | |
Modified residuei | 331 | PhosphoserineBy similarity | 1 | |
Modified residuei | 335 | PhosphoserineBy similarity | 1 | |
Modified residuei | 345 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 346 | PhosphoserineBy similarity | 1 | |
Modified residuei | 349 | PhosphoserineBy similarity | 1 | |
Modified residuei | 350 | PhosphoserineBy similarity | 1 | |
Modified residuei | 355 | PhosphoserineBy similarity | 1 | |
Modified residuei | 360 | PhosphoserineBy similarity | 1 | |
Modified residuei | 362 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated.1 Publication
Extensively phosphorylated.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9Y5K3 |
jPOSTi | Q9Y5K3 |
MassIVEi | Q9Y5K3 |
PaxDbi | Q9Y5K3 |
PeptideAtlasi | Q9Y5K3 |
PRIDEi | Q9Y5K3 |
ProteomicsDBi | 19606 86427 [Q9Y5K3-1] 86428 [Q9Y5K3-2] 86429 [Q9Y5K3-3] |
PTM databases
iPTMneti | Q9Y5K3 |
PhosphoSitePlusi | Q9Y5K3 |
Expressioni
Tissue specificityi
Highly expressed in testis, placenta, brain, ovary, liver and fetal lung.2 Publications
Expressed in brain, liver and fetal lung.1 Publication
Gene expression databases
Bgeei | ENSG00000102230, Expressed in testis and 186 other tissues |
ExpressionAtlasi | Q9Y5K3, baseline and differential |
Genevisiblei | Q9Y5K3, HS |
Organism-specific databases
HPAi | ENSG00000102230, Tissue enhanced (brain, testis) |
Interactioni
Subunit structurei
Homodimer.
By similarityBinary interactionsi
Hide detailsPCYT1B - isoform 3 [Q9Y5K3-3]
With | #Exp. | IntAct |
---|---|---|
PCYT1A [P49585] | 6 | EBI-12280028,EBI-2563309 |
itself | 3 | EBI-12280028,EBI-12280028 |
TMEM43 [Q9BTV4] | 3 | EBI-12280028,EBI-721293 |
VKORC1L1 [Q8N0U8] | 8 | EBI-12280028,EBI-11337915 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 114854, 11 interactors |
IntActi | Q9Y5K3, 4 interactors |
STRINGi | 9606.ENSP00000368439 |
Miscellaneous databases
RNActi | Q9Y5K3, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytidylyltransferase family.Curated
Phylogenomic databases
eggNOGi | KOG2804, Eukaryota |
GeneTreei | ENSGT00940000156040 |
HOGENOMi | CLU_034585_4_2_1 |
InParanoidi | Q9Y5K3 |
OMAi | TSCQCRA |
OrthoDBi | 1172502at2759 |
PhylomeDBi | Q9Y5K3 |
TreeFami | TF106336 |
Family and domain databases
CDDi | cd02174, CCT, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR041723, CCT IPR004821, Cyt_trans-like IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF01467, CTP_transf_like, 1 hit |
TIGRFAMsi | TIGR00125, cyt_tran_rel, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 2 (identifier: Q9Y5K3-1) [UniParc]FASTAAdd to basket
Also known as: Beta-21 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPVVTTDAES ETGIPKSLSN EPPSETMEEI EHTCPQPRLT LTAPAPFADE
60 70 80 90 100
TNCQCQAPHE KLTIAQARLG TPADRPVRVY ADGIFDLFHS GHARALMQAK
110 120 130 140 150
TLFPNSYLLV GVCSDDLTHK FKGFTVMNEA ERYEALRHCR YVDEVIRDAP
160 170 180 190 200
WTLTPEFLEK HKIDFVAHDD IPYSSAGSDD VYKHIKEAGM FVPTQRTEGI
210 220 230 240 250
STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKRY RFQNQVDKMK
260 270 280 290 300
EKVKNVEERS KEFVNRVEEK SHDLIQKWEE KSREFIGNFL ELFGPDGAWK
310 320 330 340 350
QMFQERSSRM LQALSPKQSP VSSPTRSRSP SRSPSPTFSW LPLKTSPPSS
360
PKAASASISS MSEGDEDEK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF2Z2B1 | F2Z2B1_HUMAN | Choline-phosphate cytidylyltransfer... | PCYT1B | 177 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 154 | T → S in BAG59022 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044659 | 1 – 39 | MPVVT…PQPRL → MVGHQECIMEEDNRAPQLWR K in isoform 4. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_020045 | 1 – 16 | Missing in isoform 3. 1 PublicationAdd BLAST | 16 | |
Alternative sequenceiVSP_020046 | 17 – 39 | SLSNE…PQPRL → MVGNQECIMEEDNRAPQLWR K in isoform 3. 1 PublicationAdd BLAST | 23 | |
Alternative sequenceiVSP_001226 | 321 – 369 | VSSPT…DEDEK → LKSWARCRDF in isoform 1. 1 PublicationAdd BLAST | 49 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF052510 mRNA Translation: AAC39754.1 AF148464 mRNA Translation: AAD35088.1 AK296333 mRNA Translation: BAG59022.1 AK315323 mRNA Translation: BAG37725.1 EU181262 Genomic DNA Translation: ABW03924.1 AC079168 Genomic DNA No translation available. CH471074 Genomic DNA Translation: EAW99021.1 BC045634 mRNA Translation: AAH45634.2 |
CCDSi | CCDS14213.1 [Q9Y5K3-1] CCDS55391.1 [Q9Y5K3-2] CCDS55392.1 [Q9Y5K3-4] |
RefSeqi | NP_001156736.1, NM_001163264.1 [Q9Y5K3-4] NP_001156737.1, NM_001163265.1 [Q9Y5K3-2] NP_004836.2, NM_004845.4 [Q9Y5K3-1] |
Genome annotation databases
Ensembli | ENST00000356768; ENSP00000349211; ENSG00000102230 [Q9Y5K3-2] ENST00000379144; ENSP00000368439; ENSG00000102230 [Q9Y5K3-1] ENST00000379145; ENSP00000368440; ENSG00000102230 [Q9Y5K3-4] |
GeneIDi | 9468 |
KEGGi | hsa:9468 |
UCSCi | uc004dbi.4, human [Q9Y5K3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
SeattleSNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF052510 mRNA Translation: AAC39754.1 AF148464 mRNA Translation: AAD35088.1 AK296333 mRNA Translation: BAG59022.1 AK315323 mRNA Translation: BAG37725.1 EU181262 Genomic DNA Translation: ABW03924.1 AC079168 Genomic DNA No translation available. CH471074 Genomic DNA Translation: EAW99021.1 BC045634 mRNA Translation: AAH45634.2 |
CCDSi | CCDS14213.1 [Q9Y5K3-1] CCDS55391.1 [Q9Y5K3-2] CCDS55392.1 [Q9Y5K3-4] |
RefSeqi | NP_001156736.1, NM_001163264.1 [Q9Y5K3-4] NP_001156737.1, NM_001163265.1 [Q9Y5K3-2] NP_004836.2, NM_004845.4 [Q9Y5K3-1] |
3D structure databases
SMRi | Q9Y5K3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 114854, 11 interactors |
IntActi | Q9Y5K3, 4 interactors |
STRINGi | 9606.ENSP00000368439 |
Chemistry databases
DrugBanki | DB00122, Choline DB14006, Choline salicylate |
SwissLipidsi | SLP:000001754 |
PTM databases
iPTMneti | Q9Y5K3 |
PhosphoSitePlusi | Q9Y5K3 |
Genetic variation databases
BioMutai | PCYT1B |
DMDMi | 12643330 |
Proteomic databases
EPDi | Q9Y5K3 |
jPOSTi | Q9Y5K3 |
MassIVEi | Q9Y5K3 |
PaxDbi | Q9Y5K3 |
PeptideAtlasi | Q9Y5K3 |
PRIDEi | Q9Y5K3 |
ProteomicsDBi | 19606 86427 [Q9Y5K3-1] 86428 [Q9Y5K3-2] 86429 [Q9Y5K3-3] |
Protocols and materials databases
Antibodypediai | 505, 54 antibodies |
Genome annotation databases
Ensembli | ENST00000356768; ENSP00000349211; ENSG00000102230 [Q9Y5K3-2] ENST00000379144; ENSP00000368439; ENSG00000102230 [Q9Y5K3-1] ENST00000379145; ENSP00000368440; ENSG00000102230 [Q9Y5K3-4] |
GeneIDi | 9468 |
KEGGi | hsa:9468 |
UCSCi | uc004dbi.4, human [Q9Y5K3-1] |
Organism-specific databases
CTDi | 9468 |
DisGeNETi | 9468 |
GeneCardsi | PCYT1B |
HGNCi | HGNC:8755, PCYT1B |
HPAi | ENSG00000102230, Tissue enhanced (brain, testis) |
MIMi | 300948, gene |
neXtProti | NX_Q9Y5K3 |
OpenTargetsi | ENSG00000102230 |
PharmGKBi | PA33100 |
VEuPathDBi | HostDB:ENSG00000102230.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2804, Eukaryota |
GeneTreei | ENSGT00940000156040 |
HOGENOMi | CLU_034585_4_2_1 |
InParanoidi | Q9Y5K3 |
OMAi | TSCQCRA |
OrthoDBi | 1172502at2759 |
PhylomeDBi | Q9Y5K3 |
TreeFami | TF106336 |
Enzyme and pathway databases
UniPathwayi | UPA00753;UER00739 |
PathwayCommonsi | Q9Y5K3 |
Reactomei | R-HSA-1483191, Synthesis of PC |
Miscellaneous databases
BioGRID-ORCSi | 9468, 2 hits in 606 CRISPR screens |
ChiTaRSi | PCYT1B, human |
GeneWikii | PCYT1B |
GenomeRNAii | 9468 |
Pharosi | Q9Y5K3, Tbio |
PROi | PR:Q9Y5K3 |
RNActi | Q9Y5K3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000102230, Expressed in testis and 186 other tissues |
ExpressionAtlasi | Q9Y5K3, baseline and differential |
Genevisiblei | Q9Y5K3, HS |
Family and domain databases
CDDi | cd02174, CCT, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR041723, CCT IPR004821, Cyt_trans-like IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF01467, CTP_transf_like, 1 hit |
TIGRFAMsi | TIGR00125, cyt_tran_rel, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PCY1B_HUMAN | |
Accessioni | Q9Y5K3Primary (citable) accession number: Q9Y5K3 Secondary accession number(s): A8IX00 Q86XC9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | April 7, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families