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Protein

Kallikrein-4

Gene

KLK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a major role in enamel formation (PubMed:15235027). Required during the maturation stage of tooth development for clearance of enamel proteins and normal structural patterning of the crystalline matrix (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Zinc1 Publication1
Active sitei71Charge relay system1 Publication1
Metal bindingi91Zinc1 Publication1
Active sitei116Charge relay system1 Publication1
Active sitei207Charge relay system1 Publication1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: ProtInc

GO - Biological processi

  • amelogenesis Source: UniProtKB
  • biomineral tissue development Source: UniProtKB-KW
  • proteolysis Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processBiomineralization
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.21.B12 2681
SIGNORiQ9Y5K2

Protein family/group databases

MEROPSiS01.251

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-4 (EC:3.4.21.-)
Alternative name(s):
Enamel matrix serine proteinase 1
Kallikrein-like protein 1
Short name:
KLK-L1
Prostase
Serine protease 17
Gene namesi
Name:KLK4
Synonyms:EMSP1, PRSS17, PSTS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000167749.11
HGNCiHGNC:6365 KLK4
MIMi603767 gene
neXtProtiNX_Q9Y5K2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Involvement in diseasei

Amelogenesis imperfecta, hypomaturation type, 2A1 (AI2A1)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
See also OMIM:204700

Keywords - Diseasei

Amelogenesis imperfecta

Organism-specific databases

DisGeNETi9622
MalaCardsiKLK4
MIMi204700 phenotype
OpenTargetsiENSG00000167749
Orphaneti100033 Hypomaturation amelogenesis imperfecta
PharmGKBiPA30154

Chemistry databases

ChEMBLiCHEMBL4446
GuidetoPHARMACOLOGYi2374

Polymorphism and mutation databases

BioMutaiKLK4
DMDMi317373372

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
PropeptideiPRO_000002793727 – 301 Publication4
ChainiPRO_000002793831 – 254Kallikrein-4Add BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 167PROSITE-ProRule annotation1 Publication
Disulfide bondi56 ↔ 72PROSITE-ProRule annotation1 Publication
Disulfide bondi141 ↔ 241PROSITE-ProRule annotation1 Publication
Disulfide bondi148 ↔ 213PROSITE-ProRule annotation1 Publication
Glycosylationi169N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi178 ↔ 192PROSITE-ProRule annotation1 Publication
Disulfide bondi203 ↔ 228PROSITE-ProRule annotation1 Publication

Post-translational modificationi

N-glycosylated. The N-glycan structures are of complex diantennary or triantennary type, which may be further modified with up to 2 sialic acid residues.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiQ9Y5K2
PaxDbiQ9Y5K2
PeptideAtlasiQ9Y5K2
PRIDEiQ9Y5K2
ProteomicsDBi86426

PTM databases

PhosphoSitePlusiQ9Y5K2

Expressioni

Tissue specificityi

Expressed in prostate.

Gene expression databases

BgeeiENSG00000167749 Expressed in 109 organ(s), highest expression level in prostate gland
CleanExiHS_KLK4
ExpressionAtlasiQ9Y5K2 baseline and differential
GenevisibleiQ9Y5K2 HS

Organism-specific databases

HPAiHPA051839

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TYRO3Q064183EBI-10224152,EBI-3951628

Protein-protein interaction databases

BioGridi114982, 5 interactors
IntActiQ9Y5K2, 1 interactor
STRINGi9606.ENSP00000326159

Chemistry databases

BindingDBiQ9Y5K2

Structurei

Secondary structure

1254
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y5K2
SMRiQ9Y5K2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y5K2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 252Peptidase S1PROSITE-ProRule annotationAdd BLAST222

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00920000149034
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiQ9Y5K2
KOiK08666
PhylomeDBiQ9Y5K2
TreeFamiTF331065

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y5K2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATAGNPWGW FLGYLILGVA GSLVSGSCSQ IINGEDCSPH SQPWQAALVM
60 70 80 90 100
ENELFCSGVL VHPQWVLSAA HCFQNSYTIG LGLHSLEADQ EPGSQMVEAS
110 120 130 140 150
LSVRHPEYNR PLLANDLMLI KLDESVSESD TIRSISIASQ CPTAGNSCLV
160 170 180 190 200
SGWGLLANGR MPTVLQCVNV SVVSEEVCSK LYDPLYHPSM FCAGGGHDQK
210 220 230 240 250
DSCNGDSGGP LICNGYLQGL VSFGKAPCGQ VGVPGVYTNL CKFTEWIEKT

VQAS
Length:254
Mass (Da):27,032
Last modified:January 11, 2011 - v2
Checksum:i9C5E4722AFF70CB8
GO
Isoform 2 (identifier: Q9Y5K2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.
     160-254: Missing.

Show »
Length:110
Mass (Da):11,858
Checksum:iB6F9C135EA93B116
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0C4DFQ5A0A0C4DFQ5_HUMAN
Kallikrein 4 (Prostase, enamel matr...
KLK4 KLNB1, hCG_1641510
254Annotation score:
Q5BQA0Q5BQA0_HUMAN
Kallikrein-4
KLK4
75Annotation score:
M0QYN5M0QYN5_HUMAN
Kallikrein-4
KLK4
71Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02836422S → A1 PublicationCorresponds to variant dbSNP:rs1654551Ensembl.1
Natural variantiVAR_033009159G → D. Corresponds to variant dbSNP:rs34626614Ensembl.1
Natural variantiVAR_028365197H → Q7 PublicationsCorresponds to variant dbSNP:rs2569527Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0566291 – 49Missing in isoform 2. 1 PublicationAdd BLAST49
Alternative sequenceiVSP_056630160 – 254Missing in isoform 2. 1 PublicationAdd BLAST95

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113140 mRNA Translation: AAD21580.1
AF113141 Genomic DNA Translation: AAD21581.1
AF135023 Genomic DNA Translation: AAD26424.2
AF148532 Genomic DNA Translation: AAD38019.1
AF243527 Genomic DNA Translation: AAG33357.1
AF228497 Genomic DNA Translation: AAF70620.1
AF259969 mRNA Translation: AAF81227.1
AF259971 mRNA Translation: AAK71706.1
AC037199 Genomic DNA No translation available.
BC069325 mRNA Translation: AAH69325.1
BC069403 mRNA Translation: AAH69403.1
BC069429 mRNA Translation: AAH69429.1
BC069489 mRNA Translation: AAH69489.1
BC096175 mRNA Translation: AAH96175.1
BC096178 mRNA Translation: AAH96178.1
AF126401 mRNA Translation: AAG43246.1
CCDSiCCDS12809.1 [Q9Y5K2-1]
RefSeqiNP_001289890.1, NM_001302961.1
NP_004908.4, NM_004917.4
UniGeneiHs.218366

Genome annotation databases

EnsembliENST00000431178; ENSP00000399448; ENSG00000167749 [Q9Y5K2-2]
GeneIDi9622
KEGGihsa:9622
UCSCiuc002pty.2 human [Q9Y5K2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113140 mRNA Translation: AAD21580.1
AF113141 Genomic DNA Translation: AAD21581.1
AF135023 Genomic DNA Translation: AAD26424.2
AF148532 Genomic DNA Translation: AAD38019.1
AF243527 Genomic DNA Translation: AAG33357.1
AF228497 Genomic DNA Translation: AAF70620.1
AF259969 mRNA Translation: AAF81227.1
AF259971 mRNA Translation: AAK71706.1
AC037199 Genomic DNA No translation available.
BC069325 mRNA Translation: AAH69325.1
BC069403 mRNA Translation: AAH69403.1
BC069429 mRNA Translation: AAH69429.1
BC069489 mRNA Translation: AAH69489.1
BC096175 mRNA Translation: AAH96175.1
BC096178 mRNA Translation: AAH96178.1
AF126401 mRNA Translation: AAG43246.1
CCDSiCCDS12809.1 [Q9Y5K2-1]
RefSeqiNP_001289890.1, NM_001302961.1
NP_004908.4, NM_004917.4
UniGeneiHs.218366

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BDGX-ray1.95A/B31-253[»]
2BDHX-ray3.00A/B/C/D31-253[»]
2BDIX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P31-253[»]
4K1EX-ray1.30A31-253[»]
4K8YX-ray1.00A31-253[»]
4KELX-ray1.15A31-253[»]
4KGAX-ray2.32A/B31-253[»]
ProteinModelPortaliQ9Y5K2
SMRiQ9Y5K2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114982, 5 interactors
IntActiQ9Y5K2, 1 interactor
STRINGi9606.ENSP00000326159

Chemistry databases

BindingDBiQ9Y5K2
ChEMBLiCHEMBL4446
GuidetoPHARMACOLOGYi2374

Protein family/group databases

MEROPSiS01.251

PTM databases

PhosphoSitePlusiQ9Y5K2

Polymorphism and mutation databases

BioMutaiKLK4
DMDMi317373372

Proteomic databases

EPDiQ9Y5K2
PaxDbiQ9Y5K2
PeptideAtlasiQ9Y5K2
PRIDEiQ9Y5K2
ProteomicsDBi86426

Protocols and materials databases

DNASUi9622
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000431178; ENSP00000399448; ENSG00000167749 [Q9Y5K2-2]
GeneIDi9622
KEGGihsa:9622
UCSCiuc002pty.2 human [Q9Y5K2-1]

Organism-specific databases

CTDi9622
DisGeNETi9622
EuPathDBiHostDB:ENSG00000167749.11
GeneCardsiKLK4
H-InvDBiHIX0039968
HGNCiHGNC:6365 KLK4
HPAiHPA051839
MalaCardsiKLK4
MIMi204700 phenotype
603767 gene
neXtProtiNX_Q9Y5K2
OpenTargetsiENSG00000167749
Orphaneti100033 Hypomaturation amelogenesis imperfecta
PharmGKBiPA30154
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00920000149034
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiQ9Y5K2
KOiK08666
PhylomeDBiQ9Y5K2
TreeFamiTF331065

Enzyme and pathway databases

BRENDAi3.4.21.B12 2681
SIGNORiQ9Y5K2

Miscellaneous databases

ChiTaRSiKLK4 human
EvolutionaryTraceiQ9Y5K2
GeneWikiiKLK4
GenomeRNAii9622
PROiPR:Q9Y5K2
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167749 Expressed in 109 organ(s), highest expression level in prostate gland
CleanExiHS_KLK4
ExpressionAtlasiQ9Y5K2 baseline and differential
GenevisibleiQ9Y5K2 HS

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKLK4_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5K2
Secondary accession number(s): Q4VB16
, Q96RU5, Q9GZL6, Q9UBJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 11, 2011
Last modified: September 12, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  7. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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