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UniProtKB - Q9Y5B9 (SP16H_HUMAN)

Protein

FACT complex subunit SPT16

Gene

SUPT16H

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).6 Publications

Caution

Although related to the peptidase M24 family, this protein lacks conserved active site residues suggesting that it may lack peptidase activity.Curated

GO - Molecular functioni

  • histone binding Source: GO_Central
  • nucleosome binding Source: GO_Central
  • RNA binding Source: UniProtKB
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Biological processDNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-75955 RNA Polymerase II Transcription Elongation

Protein family/group databases

MEROPSiM24.974

Names & Taxonomyi

Protein namesi
Recommended name:
FACT complex subunit SPT16
Alternative name(s):
Chromatin-specific transcription elongation factor 140 kDa subunit
FACT 140 kDa subunit
FACTp140
Facilitates chromatin transcription complex subunit SPT16
Short name:
hSPT16
Gene namesi
Name:SUPT16H
Synonyms:FACT140, FACTP140
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000092201.9
HGNCiHGNC:11465 SUPT16H
MIMi605012 gene
neXtProtiNX_Q9Y5B9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi11198
OpenTargetsiENSG00000092201
PharmGKBiPA36251

Polymorphism and mutation databases

BioMutaiSUPT16H
DMDMi74753511

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00002451692 – 1047FACT complex subunit SPT16Add BLAST1046

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources2 Publications1
Modified residuei139N6-acetyllysineCombined sources1
Modified residuei188PhosphoserineCombined sources1
Modified residuei196N6-acetyllysineCombined sources1
Modified residuei223N6-acetyllysineCombined sources1
Modified residuei455PhosphoserineCombined sources1
Cross-linki497Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei508PhosphoserineCombined sources1
Modified residuei513N6-acetyllysine; alternateCombined sources1
Cross-linki513Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki647Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei650PhosphoserineCombined sources1
Modified residuei658PhosphoserineCombined sources1
Modified residuei732N6-acetyllysineCombined sources1
Modified residuei786N6-acetyllysineCombined sources1
Modified residuei903PhosphothreonineCombined sources1
Modified residuei904N6-acetyllysineCombined sources1
Modified residuei979PhosphoserineCombined sources1
Modified residuei982PhosphoserineCombined sources1
Modified residuei986PhosphoserineCombined sources1
Modified residuei1015PhosphoserineCombined sources1

Post-translational modificationi

ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.

Keywords - PTMi

Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Y5B9
MaxQBiQ9Y5B9
PaxDbiQ9Y5B9
PeptideAtlasiQ9Y5B9
PRIDEiQ9Y5B9
ProteomicsDBi86338

PTM databases

iPTMnetiQ9Y5B9
PhosphoSitePlusiQ9Y5B9
SwissPalmiQ9Y5B9

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000092201 Expressed in 156 organ(s), highest expression level in kidney
CleanExiHS_SUPT16H
ExpressionAtlasiQ9Y5B9 baseline and differential
GenevisibleiQ9Y5B9 HS

Organism-specific databases

HPAiCAB022551
HPA049787

Interactioni

Subunit structurei

Interacts with MYOG (via C-terminal region) (By similarity). Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H (PubMed:10421373). Also component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B (PubMed:11239457, PubMed:12393879). Interacts with NEK9 (PubMed:14660563). Binds to histone H2A-H2B (PubMed:10421373). Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1 (PubMed:27499292). Interacts with GTF2E2 (PubMed:10792464).By similarity6 Publications
(Microbial infection) Interacts with Herpes simplex virus 1 (HHV-1) protein ICP22; this interaction relocalizes the FACT complex to viral genomes in infected cells.1 Publication

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi116367, 166 interactors
ComplexPortaliCPX-419 FACT complex
CORUMiQ9Y5B9
DIPiDIP-42757N
IntActiQ9Y5B9, 63 interactors
MINTiQ9Y5B9
STRINGi9606.ENSP00000216297

Structurei

Secondary structure

11047
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y5B9
SMRiQ9Y5B9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili432 – 507Sequence analysisAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi926 – 1011Glu-rich (acidic)Add BLAST86

Domaini

The C-terminal Glu-rich acidic region is essential for FACT activity.1 Publication

Sequence similaritiesi

Belongs to the peptidase M24 family. SPT16 subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1189 Eukaryota
COG5406 LUCA
GeneTreeiENSGT00390000014495
HOGENOMiHOG000209079
HOVERGENiHBG092544
InParanoidiQ9Y5B9
OMAiEPPFMVI
OrthoDBiEOG091G044Q
PhylomeDBiQ9Y5B9
TreeFamiTF300341

Family and domain databases

CDDicd01091 CDC68-like, 1 hit
Gene3Di2.30.29.30, 1 hit
3.40.350.10, 1 hit
InterProiView protein in InterPro
IPR029149 Creatin/AminoP/Spt16_NTD
IPR036005 Creatinase/aminopeptidase-like
IPR013719 DUF1747
IPR029148 FACT-Spt16_Nlobe
IPR013953 FACT_Spt16p
IPR000994 Pept_M24
IPR011993 PH-like_dom_sf
IPR033825 SPT16
PfamiView protein in Pfam
PF14826 FACT-Spt16_Nlob, 1 hit
PF00557 Peptidase_M24, 1 hit
PF08512 Rtt106, 1 hit
PF08644 SPT16, 1 hit
SMARTiView protein in SMART
SM01285 FACT-Spt16_Nlob, 1 hit
SM01287 Rtt106, 1 hit
SM01286 SPT16, 1 hit
SUPFAMiSSF55920 SSF55920, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9Y5B9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVTLDKDAY YRRVKRLYSN WRKGEDEYAN VDAIVVSVGV DEEIVYAKST 
        60         70         80         90        100
ALQTWLFGYE LTDTIMVFCD DKIIFMASKK KVEFLKQIAN TKGNENANGA 
       110        120        130        140        150
PAITLLIREK NESNKSSFDK MIEAIKESKN GKKIGVFSKD KFPGEFMKSW 
       160        170        180        190        200
NDCLNKEGFD KIDISAVVAY TIAVKEDGEL NLMKKAASIT SEVFNKFFKE 
       210        220        230        240        250
RVMEIVDADE KVRHSKLAES VEKAIEEKKY LAGADPSTVE MCYPPIIQSG 
       260        270        280        290        300
GNYNLKFSVV SDKNHMHFGA ITCAMGIRFK SYCSNLVRTL MVDPSQEVQE 
       310        320        330        340        350
NYNFLLQLQE ELLKELRHGV KICDVYNAVM DVVKKQKPEL LNKITKNLGF 
       360        370        380        390        400
GMGIEFREGS LVINSKNQYK LKKGMVFSIN LGFSDLTNKE GKKPEEKTYA 
       410        420        430        440        450
LFIGDTVLVD EDGPATVLTS VKKKVKNVGI FLKNEDEEEE EEEKDEAEDL 
       460        470        480        490        500
LGRGSRAALL TERTRNEMTA EEKRRAHQKE LAAQLNEEAK RRLTEQKGEQ 
       510        520        530        540        550
QIQKARKSNV SYKNPSLMPK EPHIREMKIY IDKKYETVIM PVFGIATPFH 
       560        570        580        590        600
IATIKNISMS VEGDYTYLRI NFYCPGSALG RNEGNIFPNP EATFVKEITY 
       610        620        630        640        650
RASNIKAPGE QTVPALNLQN AFRIIKEVQK RYKTREAEEK EKEGIVKQDS 
       660        670        680        690        700
LVINLNRSNP KLKDLYIRPN IAQKRMQGSL EAHVNGFRFT SVRGDKVDIL 
       710        720        730        740        750
YNNIKHALFQ PCDGEMIIVL HFHLKNAIMF GKKRHTDVQF YTEVGEITTD 
       760        770        780        790        800
LGKHQHMHDR DDLYAEQMER EMRHKLKTAF KNFIEKVEAL TKEELEFEVP 
       810        820        830        840        850
FRDLGFNGAP YRSTCLLQPT SSALVNATEW PPFVVTLDEV ELIHFERVQF 
       860        870        880        890        900
HLKNFDMVIV YKDYSKKVTM INAIPVASLD PIKEWLNSCD LKYTEGVQSL 
       910        920        930        940        950
NWTKIMKTIV DDPEGFFEQG GWSFLEPEGE GSDAEEGDSE SEIEDETFNP 
       960        970        980        990       1000
SEDDYEEEEE DSDEDYSSEA EESDYSKESL GSEEESGKDW DELEEEARKA 
      1010       1020       1030       1040 
DRESRYEEEE EQSRSMSRKR KASVHSSGRG SNRGSRHSSA PPKKKRK
Length:1,047
Mass (Da):119,914
Last modified:November 1, 1999 - v1
Checksum:i3E1B23C45BDC61C2
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V401G3V401_HUMAN
FACT complex subunit SPT16
SUPT16H
81Annotation score:
G3V5A4G3V5A4_HUMAN
FACT complex subunit SPT16
SUPT16H
66Annotation score:
G3V2X0G3V2X0_HUMAN
FACT complex subunit SPT16
SUPT16H
83Annotation score:

Sequence cautioni

The sequence AAH64561 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH73849 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152961 mRNA Translation: AAD43978.1
BC000565 mRNA Translation: AAH00565.1
BC014046 mRNA Translation: AAH14046.1
BC064561 mRNA Translation: AAH64561.1 Sequence problems.
BC073849 mRNA Translation: AAH73849.1 Sequence problems.
AF164924 mRNA Translation: AAF28231.1
CCDSiCCDS9569.1
RefSeqiNP_009123.1, NM_007192.3
UniGeneiHs.213724

Genome annotation databases

EnsembliENST00000216297; ENSP00000216297; ENSG00000092201
GeneIDi11198
KEGGihsa:11198
UCSCiuc001wao.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152961 mRNA Translation: AAD43978.1
BC000565 mRNA Translation: AAH00565.1
BC014046 mRNA Translation: AAH14046.1
BC064561 mRNA Translation: AAH64561.1 Sequence problems.
BC073849 mRNA Translation: AAH73849.1 Sequence problems.
AF164924 mRNA Translation: AAF28231.1
CCDSiCCDS9569.1
RefSeqiNP_009123.1, NM_007192.3
UniGeneiHs.213724

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Z2MX-ray2.98B644-930[»]
4Z2NX-ray1.92A644-930[»]
5E5BX-ray1.84A2-432[»]
5UMTX-ray2.09A1-434[»]
5UMUX-ray1.90A/B649-926[»]
5XM2X-ray2.19A/B1-437[»]
ProteinModelPortaliQ9Y5B9
SMRiQ9Y5B9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116367, 166 interactors
ComplexPortaliCPX-419 FACT complex
CORUMiQ9Y5B9
DIPiDIP-42757N
IntActiQ9Y5B9, 63 interactors
MINTiQ9Y5B9
STRINGi9606.ENSP00000216297

Protein family/group databases

MEROPSiM24.974

PTM databases

iPTMnetiQ9Y5B9
PhosphoSitePlusiQ9Y5B9
SwissPalmiQ9Y5B9

Polymorphism and mutation databases

BioMutaiSUPT16H
DMDMi74753511

Proteomic databases

EPDiQ9Y5B9
MaxQBiQ9Y5B9
PaxDbiQ9Y5B9
PeptideAtlasiQ9Y5B9
PRIDEiQ9Y5B9
ProteomicsDBi86338

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216297; ENSP00000216297; ENSG00000092201
GeneIDi11198
KEGGihsa:11198
UCSCiuc001wao.2 human

Organism-specific databases

CTDi11198
DisGeNETi11198
EuPathDBiHostDB:ENSG00000092201.9
GeneCardsiSUPT16H
HGNCiHGNC:11465 SUPT16H
HPAiCAB022551
HPA049787
MIMi605012 gene
neXtProtiNX_Q9Y5B9
OpenTargetsiENSG00000092201
PharmGKBiPA36251
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1189 Eukaryota
COG5406 LUCA
GeneTreeiENSGT00390000014495
HOGENOMiHOG000209079
HOVERGENiHBG092544
InParanoidiQ9Y5B9
OMAiEPPFMVI
OrthoDBiEOG091G044Q
PhylomeDBiQ9Y5B9
TreeFamiTF300341

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-75955 RNA Polymerase II Transcription Elongation

Miscellaneous databases

ChiTaRSiSUPT16H human
GeneWikiiSUPT16H
GenomeRNAii11198
PROiPR:Q9Y5B9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000092201 Expressed in 156 organ(s), highest expression level in kidney
CleanExiHS_SUPT16H
ExpressionAtlasiQ9Y5B9 baseline and differential
GenevisibleiQ9Y5B9 HS

Family and domain databases

CDDicd01091 CDC68-like, 1 hit
Gene3Di2.30.29.30, 1 hit
3.40.350.10, 1 hit
InterProiView protein in InterPro
IPR029149 Creatin/AminoP/Spt16_NTD
IPR036005 Creatinase/aminopeptidase-like
IPR013719 DUF1747
IPR029148 FACT-Spt16_Nlobe
IPR013953 FACT_Spt16p
IPR000994 Pept_M24
IPR011993 PH-like_dom_sf
IPR033825 SPT16
PfamiView protein in Pfam
PF14826 FACT-Spt16_Nlob, 1 hit
PF00557 Peptidase_M24, 1 hit
PF08512 Rtt106, 1 hit
PF08644 SPT16, 1 hit
SMARTiView protein in SMART
SM01285 FACT-Spt16_Nlob, 1 hit
SM01287 Rtt106, 1 hit
SM01286 SPT16, 1 hit
SUPFAMiSSF55920 SSF55920, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSP16H_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5B9
Secondary accession number(s): Q6GMT8
, Q6P2F1, Q6PJM1, Q9NRX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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