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Protein

NEDD8 ultimate buster 1

Gene

NUB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.1 Publication

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-8951664 Neddylation

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8 ultimate buster 1
Alternative name(s):
Negative regulator of ubiquitin-like proteins 1
Renal carcinoma antigen NY-REN-18
Gene namesi
Name:NUB1
Synonyms:NYREN18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:17623 NUB1
MIMi607981 gene
neXtProtiNX_Q9Y5A7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi448A → V: No effect on NEDD8-binding. 1 Publication1
Mutagenesisi453L → A: Partial inhibition of NEDD8-binding. 1 Publication1
Mutagenesisi464L → A: Partial inhibition of NEDD8-binding. 1 Publication1
Mutagenesisi468L → A: Partial inhibition of NEDD8-binding. 1 Publication1
Mutagenesisi587L → A: Suppression of NEDD8-binding; when associated with A-464; A-468 and A-591. Suppression of NEDD8-buster function; when associated with A-591. 1 Publication1
Mutagenesisi591L → A: Suppression of NEDD8-binding; when associated with A-464; A-468 and A-587. Suppression of NEDD8-buster function; when associated with A-587. 1 Publication1

Organism-specific databases

DisGeNETi51667
OpenTargetsiENSG00000013374
PharmGKBiPA147357533

Polymorphism and mutation databases

BioMutaiNUB1
DMDMi37154888

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002109921 – 615NEDD8 ultimate buster 1Add BLAST615

Proteomic databases

EPDiQ9Y5A7
PaxDbiQ9Y5A7
PeptideAtlasiQ9Y5A7
PRIDEiQ9Y5A7
ProteomicsDBi86324
86325 [Q9Y5A7-2]

PTM databases

iPTMnetiQ9Y5A7
PhosphoSitePlusiQ9Y5A7

Expressioni

Tissue specificityi

Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.

Inductioni

By TNF, IFNG/IFN-gamma and IFNB1/IFN-beta.2 Publications

Gene expression databases

CleanExiHS_NUB1
ExpressionAtlasiQ9Y5A7 baseline and differential

Organism-specific databases

HPAiHPA021220
HPA050722
HPA053648

Interactioni

Subunit structurei

Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Isoform 1 binds to NEDD8 more efficiently than isoform 2. Interacts with AIPL1. The interaction with UBD via UBA domains facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates UBD degradation and that of its conjugates.6 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi119670, 29 interactors
CORUMiQ9Y5A7
IntActiQ9Y5A7, 8 interactors
MINTiQ9Y5A7
STRINGi9606.ENSP00000457628

Structurei

Secondary structure

1615
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y5A7
SMRiQ9Y5A7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y5A7

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini374 – 413UBA 1PROSITE-ProRule annotationAdd BLAST40
Domaini424 – 470UBA 2PROSITE-ProRule annotationAdd BLAST47
Domaini489 – 529UBA 3PROSITE-ProRule annotationAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni427 – 474NEDD8-binding 1Add BLAST48
Regioni550 – 598NEDD8-binding 2Add BLAST49

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili36 – 70Sequence analysisAdd BLAST35
Coiled coili152 – 203Sequence analysisAdd BLAST52

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi414 – 431Nuclear localization signalCuratedAdd BLAST18

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG2561 Eukaryota
ENOG410ZK3P LUCA
GeneTreeiENSGT00390000010557
HOGENOMiHOG000113882
HOVERGENiHBG045600
InParanoidiQ9Y5A7
PhylomeDBiQ9Y5A7
TreeFamiTF323449

Family and domain databases

InterProiView protein in InterPro
IPR039749 NUB1
IPR011990 TPR-like_helical_dom_sf
IPR015940 UBA
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR12948 PTHR12948, 1 hit
PfamiView protein in Pfam
PF00627 UBA, 2 hits
SMARTiView protein in SMART
SM00165 UBA, 3 hits
SUPFAMiSSF46934 SSF46934, 3 hits
SSF48452 SSF48452, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 3 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y5A7-1) [UniParc]FASTAAdd to basket
Also known as: NUB1L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAQKKYLQAK LTQFLREDRI QLWKPPYTDE NKKVGLALKD LAKQYSDRLE
60 70 80 90 100
CCENEVEKVI EEIRCKAIER GTGNDNYRTT GIATIEVFLP PRLKKDRKNL
110 120 130 140 150
LETRLHITGR ELRSKIAETF GLQENYIKIV INKKQLQLGK TLEEQGVAHN
160 170 180 190 200
VKAMVLELKQ SEEDARKNFQ LEEEEQNEAK LKEKQIQRTK RGLEILAKRA
210 220 230 240 250
AETVVDPEMT PYLDIANQTG RSIRIPPSER KALMLAMGYH EKGRAFLKRK
260 270 280 290 300
EYGIALPCLL DADKYFCECC RELLDTVDNY AVLQLDIVWC YFRLEQLECL
310 320 330 340 350
DDAEKKLNLA QKCFKNCYGE NHQRLVHIKG NCGKEKVLFL RLYLLQGIRN
360 370 380 390 400
YHSGNDVEAY EYLNKARQLF KELYIDPSKV DNLLQLGFTA QEARLGLRAC
410 420 430 440 450
DGNVDHAATH ITNRREELAQ IRKEEKEKKR RRLENIRFLK GMGYSTHAAQ
460 470 480 490 500
QVLHAASGNL DEALKILLSN PQMWWLNDSN PETDNRQESP SQENIDRLVY
510 520 530 540 550
MGFDALVAEA ALRVFRGNVQ LAAQTLAHNG GSLPPELPLS PEDSLSPPAT
560 570 580 590 600
SPSDSAGTSS ASTDEDMETE AVNEILEDIP EHEEDYLDST LEDEEIIIAE
610
YLSYVENRKS ATKKN
Length:615
Mass (Da):70,538
Last modified:September 26, 2003 - v2
Checksum:iD70FD6B21F4CD42E
GO
Isoform 2 (identifier: Q9Y5A7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     452-465: Missing.

Show »
Length:601
Mass (Da):69,119
Checksum:i05F92632C2FCB547
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BM14H3BM14_HUMAN
NEDD8 ultimate buster 1
NUB1 NYREN18, hCG_19587
625Annotation score:
H3BM74H3BM74_HUMAN
NEDD8 ultimate buster 1
NUB1
639Annotation score:
C9JRT6C9JRT6_HUMAN
NEDD8 ultimate buster 1
NUB1
254Annotation score:
C9J7X7C9J7X7_HUMAN
NEDD8 ultimate buster 1
NUB1
154Annotation score:
F8WDB6F8WDB6_HUMAN
NEDD8 ultimate buster 1
NUB1
267Annotation score:
H7C5E1H7C5E1_HUMAN
NEDD8 ultimate buster 1
NUB1
128Annotation score:
F8WDL9F8WDL9_HUMAN
NEDD8 ultimate buster 1
NUB1
149Annotation score:
H7C5N1H7C5N1_HUMAN
NEDD8 ultimate buster 1
NUB1
202Annotation score:

Sequence cautioni

The sequence AAD42865 differs from that shown. Reason: Frameshift at positions 365 and 393.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05736913Q → R. Corresponds to variant dbSNP:rs2302131Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008335452 – 465Missing in isoform 2. 4 PublicationsAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155099 mRNA Translation: AAD42865.1 Frameshift.
AF300717 mRNA Translation: AAK21001.1
AF459743 mRNA Translation: AAO14547.1
AY129295 mRNA Translation: AAN01355.1
AC005486 Genomic DNA Translation: AAS02030.1
BC046354 mRNA Translation: AAH46354.1
AF108083 Genomic DNA Translation: AAC82474.2
CCDSiCCDS87565.1 [Q9Y5A7-1]
RefSeqiNP_001230280.1, NM_001243351.1
NP_057202.3, NM_016118.4
XP_005250068.1, XM_005250011.2
XP_016867795.1, XM_017012306.1 [Q9Y5A7-2]
UniGeneiHs.647082

Genome annotation databases

EnsembliENST00000470229; ENSP00000418234; ENSG00000013374 [Q9Y5A7-1]
GeneIDi51667
KEGGihsa:51667

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155099 mRNA Translation: AAD42865.1 Frameshift.
AF300717 mRNA Translation: AAK21001.1
AF459743 mRNA Translation: AAO14547.1
AY129295 mRNA Translation: AAN01355.1
AC005486 Genomic DNA Translation: AAS02030.1
BC046354 mRNA Translation: AAH46354.1
AF108083 Genomic DNA Translation: AAC82474.2
CCDSiCCDS87565.1 [Q9Y5A7-1]
RefSeqiNP_001230280.1, NM_001243351.1
NP_057202.3, NM_016118.4
XP_005250068.1, XM_005250011.2
XP_016867795.1, XM_017012306.1 [Q9Y5A7-2]
UniGeneiHs.647082

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WJUNMR-A75-161[»]
ProteinModelPortaliQ9Y5A7
SMRiQ9Y5A7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119670, 29 interactors
CORUMiQ9Y5A7
IntActiQ9Y5A7, 8 interactors
MINTiQ9Y5A7
STRINGi9606.ENSP00000457628

PTM databases

iPTMnetiQ9Y5A7
PhosphoSitePlusiQ9Y5A7

Polymorphism and mutation databases

BioMutaiNUB1
DMDMi37154888

Proteomic databases

EPDiQ9Y5A7
PaxDbiQ9Y5A7
PeptideAtlasiQ9Y5A7
PRIDEiQ9Y5A7
ProteomicsDBi86324
86325 [Q9Y5A7-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000470229; ENSP00000418234; ENSG00000013374 [Q9Y5A7-1]
GeneIDi51667
KEGGihsa:51667

Organism-specific databases

CTDi51667
DisGeNETi51667
GeneCardsiNUB1
H-InvDBiHIX0019486
HGNCiHGNC:17623 NUB1
HPAiHPA021220
HPA050722
HPA053648
MIMi607981 gene
neXtProtiNX_Q9Y5A7
OpenTargetsiENSG00000013374
PharmGKBiPA147357533
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2561 Eukaryota
ENOG410ZK3P LUCA
GeneTreeiENSGT00390000010557
HOGENOMiHOG000113882
HOVERGENiHBG045600
InParanoidiQ9Y5A7
PhylomeDBiQ9Y5A7
TreeFamiTF323449

Enzyme and pathway databases

ReactomeiR-HSA-8951664 Neddylation

Miscellaneous databases

ChiTaRSiNUB1 human
EvolutionaryTraceiQ9Y5A7
GeneWikiiNUB1
GenomeRNAii51667
PROiPR:Q9Y5A7
SOURCEiSearch...

Gene expression databases

CleanExiHS_NUB1
ExpressionAtlasiQ9Y5A7 baseline and differential

Family and domain databases

InterProiView protein in InterPro
IPR039749 NUB1
IPR011990 TPR-like_helical_dom_sf
IPR015940 UBA
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR12948 PTHR12948, 1 hit
PfamiView protein in Pfam
PF00627 UBA, 2 hits
SMARTiView protein in SMART
SM00165 UBA, 3 hits
SUPFAMiSSF46934 SSF46934, 3 hits
SSF48452 SSF48452, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNUB1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5A7
Secondary accession number(s): O95422
, Q75MR9, Q8IX22, Q9BXR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 26, 2003
Last modified: November 7, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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