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Protein

Ankyrin repeat and SOCS box protein 4

Gene

ASB4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes differentiation and maturation of the vascular lineage by an oxygen-dependent mechanism (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei256Essential for interaction with HIF1ANBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat and SOCS box protein 4
Short name:
ASB-4
Gene namesi
Name:ASB4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000005981.12
HGNCiHGNC:16009 ASB4
MIMi605761 gene
neXtProtiNX_Q9Y574

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi51666
OpenTargetsiENSG00000005981
PharmGKBiPA25032

Polymorphism and mutation databases

BioMutaiASB4
DMDMi20532003

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000669281 – 426Ankyrin repeat and SOCS box protein 4Add BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei246(3S)-3-hydroxyasparagine; by HIF1ANBy similarity1

Post-translational modificationi

Hydroxylation at Asn-246 by HIF1AN may provide an oxygen-dependent regulation mechanism for the function of ASB4 in promoting vascular differentiation.By similarity

Keywords - PTMi

Hydroxylation

Proteomic databases

PaxDbiQ9Y574
PeptideAtlasiQ9Y574
PRIDEiQ9Y574
ProteomicsDBi86304
86305 [Q9Y574-2]

PTM databases

iPTMnetiQ9Y574
PhosphoSitePlusiQ9Y574

Expressioni

Gene expression databases

BgeeiENSG00000005981 Expressed in 113 organ(s), highest expression level in adrenal tissue
CleanExiHS_ASB4
GenevisibleiQ9Y574 HS

Organism-specific databases

HPAiHPA055240

Interactioni

Subunit structurei

Interacts with HIF1AN. Component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex formed of CUL2 or CUL5, Elongin BC (ELOB and ELOC), RBX1 and ASB4.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119669, 27 interactors
IntActiQ9Y574, 1 interactor
STRINGi9606.ENSP00000321388

Structurei

3D structure databases

ProteinModelPortaliQ9Y574
SMRiQ9Y574
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati74 – 103ANK 1Add BLAST30
Repeati106 – 135ANK 2Add BLAST30
Repeati139 – 168ANK 3Add BLAST30
Repeati174 – 203ANK 4Add BLAST30
Repeati207 – 247ANK 5Add BLAST41
Repeati251 – 280ANK 6Add BLAST30
Domaini371 – 423SOCS boxPROSITE-ProRule annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 239Essential for interaction with HIF1ANBy similarity2
Regioni244 – 246Essential for interaction with HIF1ANBy similarity3

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes.By similarity

Sequence similaritiesi

Belongs to the ankyrin SOCS box (ASB) family.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00390000011960
HOGENOMiHOG000232066
HOVERGENiHBG024357
InParanoidiQ9Y574
KOiK10326
OMAiHHLICRM
OrthoDBiEOG091G064L
PhylomeDBiQ9Y574
TreeFamiTF331836

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR001496 SOCS_box
IPR036036 SOCS_box-like_dom_sf
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF07525 SOCS_box, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SM00969 SOCS_box, 1 hit
SUPFAMiSSF158235 SSF158235, 1 hit
SSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 5 hits
PS50225 SOCS, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9Y574-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDGTTAPVTK SGAAKLVKRN FLEALKSNDF GKLKAILIQR QIDVDTVFEV
60 70 80 90 100
EDENMVLASY KQGYWLPSYK LKSSWATGLH LSVLFGHVEC LLVLLDHNAT
110 120 130 140 150
INCRPNGKTP LHVACEMANV DCVKILCDRG AKLNCYSLSG HTALHFCTTP
160 170 180 190 200
SSILCAKQLV WRGANVNMKT NNQDEETPLH TAAHFGLSEL VAFYVEHGAI
210 220 230 240 250
VDSVNAHMET PLAIAAYWAL RFKEQEYSTE HHLVCRMLLD YKAEVNARDD
260 270 280 290 300
DFKSPLHKAA WNCDHVLMHM MLEAGAEANL MDINGCAAIQ YVLKVTSVRP
310 320 330 340 350
AAQPEICYQL LLNHGAARIY PPQFHKVIQA CHSCPKAIEV VVNAYEHIRW
360 370 380 390 400
NTKWRRAIPD DDLEKYWDFY HSLFTVCCNS PRTLMHLSRC AIRRTLHNRC
410 420
HRAIPLLSLP LSLKKYLLLE PEGIIY
Length:426
Mass (Da):48,195
Last modified:November 1, 1999 - v1
Checksum:i4027F109A6C264A5
GO
Isoform 2 (identifier: Q9Y574-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     328-426: IQACHSCPKA...LLLEPEGIIY → RLCPVVSRLRKIQMAALSEISK

Note: No experimental confirmation available.
Show »
Length:349
Mass (Da):38,931
Checksum:i53801D22056907FD
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03351217V → L. Corresponds to variant dbSNP:rs35047380Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042668328 – 426IQACH…EGIIY → RLCPVVSRLRKIQMAALSEI SK in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156779 mRNA Translation: AAD41896.1
AC002451 Genomic DNA No translation available.
AC003079 Genomic DNA Translation: AAB83943.2
CH471091 Genomic DNA Translation: EAW76761.1
CH471091 Genomic DNA Translation: EAW76762.1
CH236949 Genomic DNA Translation: EAL24129.1
CH236949 Genomic DNA Translation: EAL24130.1
BC113479 mRNA Translation: AAI13480.1
BC113481 mRNA Translation: AAI13482.1
BC025024 mRNA Translation: AAH25024.1
CCDSiCCDS5641.1 [Q9Y574-1]
CCDS5642.1 [Q9Y574-2]
RefSeqiNP_057200.1, NM_016116.2 [Q9Y574-1]
NP_665879.1, NM_145872.2 [Q9Y574-2]
UniGeneiHs.602765
Hs.737518

Genome annotation databases

EnsembliENST00000325885; ENSP00000321388; ENSG00000005981 [Q9Y574-1]
ENST00000428113; ENSP00000397070; ENSG00000005981 [Q9Y574-2]
GeneIDi51666
KEGGihsa:51666
UCSCiuc003unx.3 human [Q9Y574-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156779 mRNA Translation: AAD41896.1
AC002451 Genomic DNA No translation available.
AC003079 Genomic DNA Translation: AAB83943.2
CH471091 Genomic DNA Translation: EAW76761.1
CH471091 Genomic DNA Translation: EAW76762.1
CH236949 Genomic DNA Translation: EAL24129.1
CH236949 Genomic DNA Translation: EAL24130.1
BC113479 mRNA Translation: AAI13480.1
BC113481 mRNA Translation: AAI13482.1
BC025024 mRNA Translation: AAH25024.1
CCDSiCCDS5641.1 [Q9Y574-1]
CCDS5642.1 [Q9Y574-2]
RefSeqiNP_057200.1, NM_016116.2 [Q9Y574-1]
NP_665879.1, NM_145872.2 [Q9Y574-2]
UniGeneiHs.602765
Hs.737518

3D structure databases

ProteinModelPortaliQ9Y574
SMRiQ9Y574
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119669, 27 interactors
IntActiQ9Y574, 1 interactor
STRINGi9606.ENSP00000321388

PTM databases

iPTMnetiQ9Y574
PhosphoSitePlusiQ9Y574

Polymorphism and mutation databases

BioMutaiASB4
DMDMi20532003

Proteomic databases

PaxDbiQ9Y574
PeptideAtlasiQ9Y574
PRIDEiQ9Y574
ProteomicsDBi86304
86305 [Q9Y574-2]

Protocols and materials databases

DNASUi51666
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325885; ENSP00000321388; ENSG00000005981 [Q9Y574-1]
ENST00000428113; ENSP00000397070; ENSG00000005981 [Q9Y574-2]
GeneIDi51666
KEGGihsa:51666
UCSCiuc003unx.3 human [Q9Y574-1]

Organism-specific databases

CTDi51666
DisGeNETi51666
EuPathDBiHostDB:ENSG00000005981.12
GeneCardsiASB4
HGNCiHGNC:16009 ASB4
HPAiHPA055240
MIMi605761 gene
neXtProtiNX_Q9Y574
OpenTargetsiENSG00000005981
PharmGKBiPA25032
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00390000011960
HOGENOMiHOG000232066
HOVERGENiHBG024357
InParanoidiQ9Y574
KOiK10326
OMAiHHLICRM
OrthoDBiEOG091G064L
PhylomeDBiQ9Y574
TreeFamiTF331836

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

GenomeRNAii51666
PROiPR:Q9Y574
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000005981 Expressed in 113 organ(s), highest expression level in adrenal tissue
CleanExiHS_ASB4
GenevisibleiQ9Y574 HS

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR001496 SOCS_box
IPR036036 SOCS_box-like_dom_sf
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF07525 SOCS_box, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SM00969 SOCS_box, 1 hit
SUPFAMiSSF158235 SSF158235, 1 hit
SSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 5 hits
PS50225 SOCS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiASB4_HUMAN
AccessioniPrimary (citable) accession number: Q9Y574
Secondary accession number(s): A4D1H6
, O14586, Q14D68, Q8TBT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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