UniProtKB - Q9Y570 (PPME1_HUMAN)
Protein
Protein phosphatase methylesterase 1
Gene
PPME1
Organism
Homo sapiens (Human)
Status
Functioni
Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.1 Publication
Catalytic activityi
- [phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + H+ + methanol2 PublicationsEC:3.1.1.892 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 156 | 1 Publication | 1 | |
Active sitei | 181 | 1 Publication | 1 | |
Active sitei | 349 | 1 Publication | 1 |
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- protein C-terminal methylesterase activity Source: HGNC-UCL
- protein kinase binding Source: Ensembl
- protein methylesterase activity Source: GO_Central
- protein phosphatase 2A binding Source: HGNC-UCL
- protein phosphatase binding Source: HGNC-UCL
- protein phosphatase inhibitor activity Source: ProtInc
- protein phosphatase regulator activity Source: HGNC-UCL
GO - Biological processi
- G2/M transition of mitotic cell cycle Source: Reactome
- protein demethylation Source: HGNC-UCL
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-16514 |
BRENDAi | 3.1.1.89, 2681 |
PathwayCommonsi | Q9Y570 |
Reactomei | R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition |
Protein family/group databases
ESTHERi | human-PPME1, PPase_methylesterase_euk |
MEROPSi | S33.984 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PPME1 Synonyms:PME1 ORF Names:PP2593, PRO0750 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30178, PPME1 |
MIMi | 611117, gene |
neXtProti | NX_Q9Y570 |
VEuPathDBi | HostDB:ENSG00000214517.8 |
Subcellular locationi
Nucleus
- nucleoplasm Source: Reactome
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 51400 |
OpenTargetsi | ENSG00000214517 |
PharmGKBi | PA142671152 |
Miscellaneous databases
Pharosi | Q9Y570, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1293320 |
GuidetoPHARMACOLOGYi | 2875 |
Genetic variation databases
BioMutai | PPME1 |
DMDMi | 47606055 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000090390 | 2 – 386 | Protein phosphatase methylesterase 1Add BLAST | 385 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | PhosphoserineCombined sources | 1 | |
Modified residuei | 16 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 16 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 42 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1.By similarity
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q9Y570 |
jPOSTi | Q9Y570 |
MassIVEi | Q9Y570 |
PaxDbi | Q9Y570 |
PeptideAtlasi | Q9Y570 |
PRIDEi | Q9Y570 |
ProteomicsDBi | 86296 [Q9Y570-1] 86297 [Q9Y570-2] 86298 [Q9Y570-3] |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00007694 |
PTM databases
iPTMneti | Q9Y570 |
MetOSitei | Q9Y570 |
PhosphoSitePlusi | Q9Y570 |
SwissPalmi | Q9Y570 |
Expressioni
Gene expression databases
Bgeei | ENSG00000214517, Expressed in frontal cortex and 235 other tissues |
ExpressionAtlasi | Q9Y570, baseline and differential |
Genevisiblei | Q9Y570, HS |
Organism-specific databases
HPAi | ENSG00000214517, Tissue enhanced (parathyroid) |
Interactioni
Subunit structurei
Binds PPP2CA and PPP2CB.
1 PublicationBinary interactionsi
Q9Y570
With | #Exp. | IntAct |
---|---|---|
PPP2CA - isoform 1 [P67775-1] | 2 | EBI-1772895,EBI-16765970 |
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- protein kinase binding Source: Ensembl
- protein phosphatase 2A binding Source: HGNC-UCL
- protein phosphatase binding Source: HGNC-UCL
Protein-protein interaction databases
BioGRIDi | 119524, 106 interactors |
CORUMi | Q9Y570 |
IntActi | Q9Y570, 29 interactors |
MINTi | Q9Y570 |
STRINGi | 9606.ENSP00000381461 |
Chemistry databases
BindingDBi | Q9Y570 |
Miscellaneous databases
RNActi | Q9Y570, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9Y570 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9Y570 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 256 – 263 | Poly-Glu | 8 |
Sequence similaritiesi
Belongs to the AB hydrolase superfamily.Curated
Phylogenomic databases
eggNOGi | KOG2564, Eukaryota |
GeneTreei | ENSGT00390000004396 |
HOGENOMi | CLU_024818_0_1_1 |
InParanoidi | Q9Y570 |
OMAi | WHIRSRT |
OrthoDBi | 911863at2759 |
PhylomeDBi | Q9Y570 |
TreeFami | TF314697 |
Family and domain databases
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000073, AB_hydrolase_1 IPR016812, PPase_methylesterase_euk |
PANTHERi | PTHR14189, PTHR14189, 1 hit |
Pfami | View protein in Pfam PF12697, Abhydrolase_6, 1 hit |
PIRSFi | PIRSF022950, PPase_methylesterase_euk, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00120, LIPASE_SER, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9Y570-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FSPVPWSQYF
60 70 80 90 100
ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII
110 120 130 140 150
SRVQCRIVAL DLRSHGETKV KNPEDLSAET MAKDVGNVVE AMYGDLPPPI
160 170 180 190 200
MLIGHSMGGA IAVHTASSNL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG
210 220 230 240 250
RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPEGSKSI
260 270 280 290 300
VEGIIEEEEE DEEGSESISK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG
310 320 330 340 350
WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ MQVLPQCGHA
360 370 380
VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC
Sequence cautioni
The sequence AAG22477 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 309 | F → S in BAA91661 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010335 | 1 – 276 | Missing in isoform 3. 1 PublicationAdd BLAST | 276 | |
Alternative sequenceiVSP_010336 | 1 – 187 | Missing in isoform 2. 1 PublicationAdd BLAST | 187 | |
Alternative sequenceiVSP_054818 | 278 | E → EGLPSETQNLLLFLQ in isoform 4. Curated | 1 | |
Alternative sequenceiVSP_010337 | 360 – 386 | AEAVA…VFPGC → SLVLSDCKRTTVRITLDVTE DKSLSLSLHCLQQLLWSLCR CSSTSSPTSPWQLLMVLVLC ICAEELLTLCYFIPGPCG in isoform 3. 1 PublicationAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF157028 mRNA Translation: AAD44976.1 AF111853 mRNA Translation: AAF16692.1 AK001381 mRNA Translation: BAA91661.1 AK022725 mRNA Translation: BAG51108.1 AK123288 mRNA Translation: BAC85574.1 AP000577 Genomic DNA No translation available. AP002392 Genomic DNA No translation available. CH471076 Genomic DNA Translation: EAW74932.1 BC003046 mRNA Translation: AAH03046.1 BC050705 mRNA Translation: AAH50705.1 AF193049 mRNA Translation: AAG22477.1 Sequence problems. |
CCDSi | CCDS44678.1 [Q9Y570-1] CCDS60891.1 [Q9Y570-4] |
RefSeqi | NP_001258522.1, NM_001271593.1 [Q9Y570-4] NP_057231.1, NM_016147.2 [Q9Y570-1] |
Genome annotation databases
Ensembli | ENST00000328257; ENSP00000329867; ENSG00000214517 [Q9Y570-1] ENST00000398427; ENSP00000381461; ENSG00000214517 [Q9Y570-4] |
GeneIDi | 51400 |
KEGGi | hsa:51400 |
UCSCi | uc001ouw.5, human [Q9Y570-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF157028 mRNA Translation: AAD44976.1 AF111853 mRNA Translation: AAF16692.1 AK001381 mRNA Translation: BAA91661.1 AK022725 mRNA Translation: BAG51108.1 AK123288 mRNA Translation: BAC85574.1 AP000577 Genomic DNA No translation available. AP002392 Genomic DNA No translation available. CH471076 Genomic DNA Translation: EAW74932.1 BC003046 mRNA Translation: AAH03046.1 BC050705 mRNA Translation: AAH50705.1 AF193049 mRNA Translation: AAG22477.1 Sequence problems. |
CCDSi | CCDS44678.1 [Q9Y570-1] CCDS60891.1 [Q9Y570-4] |
RefSeqi | NP_001258522.1, NM_001271593.1 [Q9Y570-4] NP_057231.1, NM_016147.2 [Q9Y570-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3C5V | X-ray | 2.00 | A | 39-386 | [»] | |
3C5W | X-ray | 2.80 | P | 284-386 | [»] | |
P | 39-238 | [»] | ||||
SMRi | Q9Y570 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119524, 106 interactors |
CORUMi | Q9Y570 |
IntActi | Q9Y570, 29 interactors |
MINTi | Q9Y570 |
STRINGi | 9606.ENSP00000381461 |
Chemistry databases
BindingDBi | Q9Y570 |
ChEMBLi | CHEMBL1293320 |
GuidetoPHARMACOLOGYi | 2875 |
Protein family/group databases
ESTHERi | human-PPME1, PPase_methylesterase_euk |
MEROPSi | S33.984 |
PTM databases
iPTMneti | Q9Y570 |
MetOSitei | Q9Y570 |
PhosphoSitePlusi | Q9Y570 |
SwissPalmi | Q9Y570 |
Genetic variation databases
BioMutai | PPME1 |
DMDMi | 47606055 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00007694 |
Proteomic databases
EPDi | Q9Y570 |
jPOSTi | Q9Y570 |
MassIVEi | Q9Y570 |
PaxDbi | Q9Y570 |
PeptideAtlasi | Q9Y570 |
PRIDEi | Q9Y570 |
ProteomicsDBi | 86296 [Q9Y570-1] 86297 [Q9Y570-2] 86298 [Q9Y570-3] |
Protocols and materials databases
Antibodypediai | 3870, 311 antibodies |
DNASUi | 51400 |
Genome annotation databases
Ensembli | ENST00000328257; ENSP00000329867; ENSG00000214517 [Q9Y570-1] ENST00000398427; ENSP00000381461; ENSG00000214517 [Q9Y570-4] |
GeneIDi | 51400 |
KEGGi | hsa:51400 |
UCSCi | uc001ouw.5, human [Q9Y570-1] |
Organism-specific databases
CTDi | 51400 |
DisGeNETi | 51400 |
GeneCardsi | PPME1 |
HGNCi | HGNC:30178, PPME1 |
HPAi | ENSG00000214517, Tissue enhanced (parathyroid) |
MIMi | 611117, gene |
neXtProti | NX_Q9Y570 |
OpenTargetsi | ENSG00000214517 |
PharmGKBi | PA142671152 |
VEuPathDBi | HostDB:ENSG00000214517.8 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2564, Eukaryota |
GeneTreei | ENSGT00390000004396 |
HOGENOMi | CLU_024818_0_1_1 |
InParanoidi | Q9Y570 |
OMAi | WHIRSRT |
OrthoDBi | 911863at2759 |
PhylomeDBi | Q9Y570 |
TreeFami | TF314697 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-16514 |
BRENDAi | 3.1.1.89, 2681 |
PathwayCommonsi | Q9Y570 |
Reactomei | R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition |
Miscellaneous databases
BioGRID-ORCSi | 51400, 161 hits in 874 CRISPR screens |
ChiTaRSi | PPME1, human |
EvolutionaryTracei | Q9Y570 |
GenomeRNAii | 51400 |
Pharosi | Q9Y570, Tchem |
PROi | PR:Q9Y570 |
RNActi | Q9Y570, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000214517, Expressed in frontal cortex and 235 other tissues |
ExpressionAtlasi | Q9Y570, baseline and differential |
Genevisiblei | Q9Y570, HS |
Family and domain databases
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000073, AB_hydrolase_1 IPR016812, PPase_methylesterase_euk |
PANTHERi | PTHR14189, PTHR14189, 1 hit |
Pfami | View protein in Pfam PF12697, Abhydrolase_6, 1 hit |
PIRSFi | PIRSF022950, PPase_methylesterase_euk, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00120, LIPASE_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPME1_HUMAN | |
Accessioni | Q9Y570Primary (citable) accession number: Q9Y570 Secondary accession number(s): B3KMU6 Q9UI18 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 179 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families