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Protein

E3 ubiquitin-protein ligase RNF114

Gene

RNF114

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase promoting the ubiquitination and degradation of the CDK inhibitor CDKN1A and probably also CDKN1B and CDKN1C. These activities stimulate cell cycle's G1-to-S phase transition and suppress cellular senescence. May play a role in spermatogenesis.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri29 – 68RING-typePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri91 – 110C2HC RNF-typePROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processDifferentiation, Spermatogenesis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF114 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 114
RING-type E3 ubiquitin transferase RNF114Curated
Zinc finger protein 228
Zinc finger protein 313
Gene namesi
Name:RNF114
Synonyms:ZNF228, ZNF313
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000124226.10
HGNCiHGNC:13094 RNF114
MIMi612451 gene
neXtProtiNX_Q9Y508

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55905
MalaCardsiRNF114
OpenTargetsiENSG00000124226
PharmGKBiPA162401502

Polymorphism and mutation databases

BioMutaiRNF114
DMDMi20141070

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000563071 – 228E3 ubiquitin-protein ligase RNF114Add BLAST228

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102N6-acetyllysineCombined sources1
Modified residuei112N6-acetyllysineCombined sources1

Post-translational modificationi

Autoubiquitinated. Polyubiquitinated in the presence of E2 enzymes UBE2D1, UBE2D2 and UBE2D3, but only monoubiquitinated in the presence of UBE2E1.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiQ9Y508
PaxDbiQ9Y508
PeptideAtlasiQ9Y508
PRIDEiQ9Y508
ProteomicsDBi86269
86270 [Q9Y508-2]
TopDownProteomicsiQ9Y508-1 [Q9Y508-1]

PTM databases

iPTMnetiQ9Y508
PhosphoSitePlusiQ9Y508

Expressioni

Tissue specificityi

Expressed in numerous tissues, including skin, CD4 lymphocytes and dendritic cells. Highest levels in testis.1 Publication

Gene expression databases

BgeeiENSG00000124226 Expressed in 238 organ(s), highest expression level in oocyte
CleanExiHS_RNF114
ExpressionAtlasiQ9Y508 baseline and differential
GenevisibleiQ9Y508 HS

Organism-specific databases

HPAiHPA021184

Interactioni

Subunit structurei

Interacts with XAF1, the interaction increases XAF1 stability and proapoptotic effects, and may regulate IFN signaling.1 Publication

Protein-protein interaction databases

BioGridi120991, 54 interactors
IntActiQ9Y508, 27 interactors
MINTiQ9Y508
STRINGi9606.ENSP00000244061

Structurei

3D structure databases

ProteinModelPortaliQ9Y508
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri29 – 68RING-typePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri91 – 110C2HC RNF-typePROSITE-ProRule annotationAdd BLAST20

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEG7 Eukaryota
ENOG4111JWC LUCA
GeneTreeiENSGT00530000063064
HOGENOMiHOG000230946
HOVERGENiHBG074331
InParanoidiQ9Y508
KOiK15697
OMAiVEHCKLS
OrthoDBiEOG091G0M3K
PhylomeDBiQ9Y508
TreeFamiTF331012

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR008598 Di19_Zn_binding_dom
IPR034734 ZF_C2HC_RNF
IPR027370 Znf-RING_LisH
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF05605 zf-Di19, 1 hit
PF13445 zf-RING_UBOX, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS51803 ZF_C2HC_RNF, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y508-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAQQRDCGG AAQLAGPAAE ADPLGRFTCP VCLEVYEKPV QVPCGHVFCS
60 70 80 90 100
ACLQECLKPK KPVCGVCRSA LAPGVRAVEL ERQIESTETS CHGCRKNFFL
110 120 130 140 150
SKIRSHVATC SKYQNYIMEG VKATIKDASL QPRNVPNRYT FPCPYCPEKN
160 170 180 190 200
FDQEGLVEHC KLFHSTDTKS VVCPICASMP WGDPNYRSAN FREHIQRRHR
210 220
FSYDTFVDYD VDEEDMMNQV LQRSIIDQ
Length:228
Mass (Da):25,694
Last modified:November 1, 1999 - v1
Checksum:iDF5A962B1EC13A21
GO
Isoform 2 (identifier: Q9Y508-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     173-191: CPICASMPWGDPNYRSANF → SEQSPCLLSVSCYRASITY
     192-228: Missing.

Note: No experimental confirmation available.
Show »
Length:191
Mass (Da):21,004
Checksum:i1F509A3749A2AE10
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A096LNV3A0A096LNV3_HUMAN
E3 ubiquitin-protein ligase RNF114
RNF114
188Annotation score:
A0A096LP02A0A096LP02_HUMAN
E3 ubiquitin-protein ligase RNF114
RNF114
206Annotation score:
A0A096LNT1A0A096LNT1_HUMAN
E3 ubiquitin-protein ligase RNF114
RNF114
49Annotation score:
A0A096LPF9A0A096LPF9_HUMAN
E3 ubiquitin-protein ligase RNF114
RNF114
83Annotation score:
A0A096LNN8A0A096LNN8_HUMAN
E3 ubiquitin-protein ligase RNF114
RNF114
70Annotation score:

Sequence cautioni

The sequence CAE45709 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13Q → H in CAE45709 (PubMed:17974005).Curated1
Sequence conflicti20E → K in CAE45709 (PubMed:17974005).Curated1
Sequence conflicti34E → V in CAE45709 (PubMed:17974005).Curated1
Sequence conflicti205T → A in CAE45709 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036843173 – 191CPICA…RSANF → SEQSPCLLSVSCYRASITY in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_036844192 – 228Missing in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF265215 mRNA Translation: AAF75763.1
BX640603 mRNA Translation: CAE45709.1 Different initiation.
BT006795 mRNA Translation: AAP35441.1
AK315638 mRNA Translation: BAG38006.1
AK301731 mRNA Translation: BAG63197.1
AL031685 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW75641.1
CH471077 Genomic DNA Translation: EAW75643.1
BC013695 mRNA Translation: AAH13695.1
BC066919 mRNA Translation: AAH66919.1
CCDSiCCDS33482.1 [Q9Y508-1]
RefSeqiNP_061153.1, NM_018683.3 [Q9Y508-1]
UniGeneiHs.144949

Genome annotation databases

EnsembliENST00000244061; ENSP00000244061; ENSG00000124226 [Q9Y508-1]
GeneIDi55905
KEGGihsa:55905
UCSCiuc002xux.4 human [Q9Y508-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF265215 mRNA Translation: AAF75763.1
BX640603 mRNA Translation: CAE45709.1 Different initiation.
BT006795 mRNA Translation: AAP35441.1
AK315638 mRNA Translation: BAG38006.1
AK301731 mRNA Translation: BAG63197.1
AL031685 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW75641.1
CH471077 Genomic DNA Translation: EAW75643.1
BC013695 mRNA Translation: AAH13695.1
BC066919 mRNA Translation: AAH66919.1
CCDSiCCDS33482.1 [Q9Y508-1]
RefSeqiNP_061153.1, NM_018683.3 [Q9Y508-1]
UniGeneiHs.144949

3D structure databases

ProteinModelPortaliQ9Y508
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120991, 54 interactors
IntActiQ9Y508, 27 interactors
MINTiQ9Y508
STRINGi9606.ENSP00000244061

PTM databases

iPTMnetiQ9Y508
PhosphoSitePlusiQ9Y508

Polymorphism and mutation databases

BioMutaiRNF114
DMDMi20141070

Proteomic databases

EPDiQ9Y508
PaxDbiQ9Y508
PeptideAtlasiQ9Y508
PRIDEiQ9Y508
ProteomicsDBi86269
86270 [Q9Y508-2]
TopDownProteomicsiQ9Y508-1 [Q9Y508-1]

Protocols and materials databases

DNASUi55905
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000244061; ENSP00000244061; ENSG00000124226 [Q9Y508-1]
GeneIDi55905
KEGGihsa:55905
UCSCiuc002xux.4 human [Q9Y508-1]

Organism-specific databases

CTDi55905
DisGeNETi55905
EuPathDBiHostDB:ENSG00000124226.10
GeneCardsiRNF114
HGNCiHGNC:13094 RNF114
HPAiHPA021184
MalaCardsiRNF114
MIMi612451 gene
neXtProtiNX_Q9Y508
OpenTargetsiENSG00000124226
PharmGKBiPA162401502
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEG7 Eukaryota
ENOG4111JWC LUCA
GeneTreeiENSGT00530000063064
HOGENOMiHOG000230946
HOVERGENiHBG074331
InParanoidiQ9Y508
KOiK15697
OMAiVEHCKLS
OrthoDBiEOG091G0M3K
PhylomeDBiQ9Y508
TreeFamiTF331012

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiRNF114 human
GenomeRNAii55905
PROiPR:Q9Y508
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000124226 Expressed in 238 organ(s), highest expression level in oocyte
CleanExiHS_RNF114
ExpressionAtlasiQ9Y508 baseline and differential
GenevisibleiQ9Y508 HS

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR008598 Di19_Zn_binding_dom
IPR034734 ZF_C2HC_RNF
IPR027370 Znf-RING_LisH
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF05605 zf-Di19, 1 hit
PF13445 zf-RING_UBOX, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS51803 ZF_C2HC_RNF, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRN114_HUMAN
AccessioniPrimary (citable) accession number: Q9Y508
Secondary accession number(s): B2RDQ9
, B4DWY5, E1P627, Q6N0B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
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