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Protein

Sperm flagellar protein 1

Gene

SPEF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium (By similarity). Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock (By similarity).By similarity

Miscellaneous

Radial intercalation is a developmentally reiterated form of migration by which cells move in a direction orthogonal to the plane of the tissue from an inner layer to an outer layer.By similarity

GO - Molecular functioni

GO - Biological processi

  • cell migration Source: UniProtKB
  • cilium-dependent cell motility Source: GO_Central
  • negative regulation of microtubule depolymerization Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm flagellar protein 1
Gene namesi
Name:SPEF1
Synonyms:C20orf28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000101222.12
HGNCiHGNC:15874 SPEF1
MIMi610674 gene
neXtProtiNX_Q9Y4P9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000101222
PharmGKBiPA162404432

Polymorphism and mutation databases

BioMutaiSPEF1
DMDMi152031688

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000794211 – 236Sperm flagellar protein 1Add BLAST236

Proteomic databases

PaxDbiQ9Y4P9
PeptideAtlasiQ9Y4P9
PRIDEiQ9Y4P9
ProteomicsDBi86242
86243 [Q9Y4P9-2]

PTM databases

PhosphoSitePlusiQ9Y4P9

Expressioni

Gene expression databases

BgeeiENSG00000101222 Expressed in 124 organ(s), highest expression level in right uterine tube
CleanExiHS_SPEF1
GenevisibleiQ9Y4P9 HS

Organism-specific databases

HPAiHPA050786

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT40Q6A1623EBI-740053,EBI-10171697

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117391, 4 interactors
IntActiQ9Y4P9, 5 interactors
STRINGi9606.ENSP00000369080

Structurei

Secondary structure

1236
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y4P9
SMRiQ9Y4P9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y4P9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 112Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST106

Phylogenomic databases

eggNOGiENOG410IYU4 Eukaryota
ENOG4111WVQ LUCA
GeneTreeiENSGT00910000144159
HOGENOMiHOG000231709
HOVERGENiHBG051156
InParanoidiQ9Y4P9
OMAiREKDYTI
OrthoDBiEOG091G0V3B
PhylomeDBiQ9Y4P9
TreeFamiTF323506

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR010441 CH_2
IPR036872 CH_dom_sf
PfamiView protein in Pfam
PF06294 CH_2, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9Y4P9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASSVDEEAL HQLYLWVDNI PLSRPKRNLS RDFSDGVLVA EVIKFYFPKM
60 70 80 90 100
VEMHNYVPAN SLQQKLSNWG HLNRKVLKRL NFSVPDDVMR KIAQCAPGVV
110 120 130 140 150
ELVLIPLRQR LEERQRRRKQ GAGSLQELAP QDGSGYMDVG VSQKARGEGV
160 170 180 190 200
PDPQGGGQLS WDRPPAPRPP AYNRALQGDP SFVLQIAEKE QELLASQETV
210 220 230
QVLQMKVRRL EHLLQLKNVR IEDLSRRLQQ AERKQR
Note: Gene prediction based on EST data. No experimental confirmation available.
Length:236
Mass (Da):26,987
Last modified:July 10, 2007 - v3
Checksum:i74FE1C07D5DE749E
GO
Isoform 2 (identifier: Q9Y4P9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-162: VSQKARGEGVPDPQGGGQLSWD → KVAFSISPSRLELSFCPSSCHL
     163-236: Missing.

Show »
Length:162
Mass (Da):18,435
Checksum:iA496B8FA659AE66B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017219141 – 162VSQKA…QLSWD → KVAFSISPSRLELSFCPSSC HL in isoform 2. 2 PublicationsAdd BLAST22
Alternative sequenceiVSP_017220163 – 236Missing in isoform 2. 2 PublicationsAdd BLAST74

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL080154 mRNA Translation: CAB45745.1
EF560745 mRNA Translation: ABQ59055.1
AL109804 Genomic DNA No translation available.
CH471133 Genomic DNA Translation: EAX10503.1
CH471133 Genomic DNA Translation: EAX10504.1
CH471133 Genomic DNA Translation: EAX10505.1
BC022476 mRNA Translation: AAH22476.1
CCDSiCCDS13063.2 [Q9Y4P9-1]
PIRiT12538
RefSeqiNP_056232.2, NM_015417.4 [Q9Y4P9-1]
UniGeneiHs.72620

Genome annotation databases

EnsembliENST00000379756; ENSP00000369080; ENSG00000101222 [Q9Y4P9-1]
GeneIDi25876
KEGGihsa:25876
UCSCiuc002wjj.4 human [Q9Y4P9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL080154 mRNA Translation: CAB45745.1
EF560745 mRNA Translation: ABQ59055.1
AL109804 Genomic DNA No translation available.
CH471133 Genomic DNA Translation: EAX10503.1
CH471133 Genomic DNA Translation: EAX10504.1
CH471133 Genomic DNA Translation: EAX10505.1
BC022476 mRNA Translation: AAH22476.1
CCDSiCCDS13063.2 [Q9Y4P9-1]
PIRiT12538
RefSeqiNP_056232.2, NM_015417.4 [Q9Y4P9-1]
UniGeneiHs.72620

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EE7NMR-A1-120[»]
ProteinModelPortaliQ9Y4P9
SMRiQ9Y4P9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117391, 4 interactors
IntActiQ9Y4P9, 5 interactors
STRINGi9606.ENSP00000369080

PTM databases

PhosphoSitePlusiQ9Y4P9

Polymorphism and mutation databases

BioMutaiSPEF1
DMDMi152031688

Proteomic databases

PaxDbiQ9Y4P9
PeptideAtlasiQ9Y4P9
PRIDEiQ9Y4P9
ProteomicsDBi86242
86243 [Q9Y4P9-2]

Protocols and materials databases

DNASUi25876
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379756; ENSP00000369080; ENSG00000101222 [Q9Y4P9-1]
GeneIDi25876
KEGGihsa:25876
UCSCiuc002wjj.4 human [Q9Y4P9-1]

Organism-specific databases

CTDi25876
EuPathDBiHostDB:ENSG00000101222.12
GeneCardsiSPEF1
HGNCiHGNC:15874 SPEF1
HPAiHPA050786
MIMi610674 gene
neXtProtiNX_Q9Y4P9
OpenTargetsiENSG00000101222
PharmGKBiPA162404432
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IYU4 Eukaryota
ENOG4111WVQ LUCA
GeneTreeiENSGT00910000144159
HOGENOMiHOG000231709
HOVERGENiHBG051156
InParanoidiQ9Y4P9
OMAiREKDYTI
OrthoDBiEOG091G0V3B
PhylomeDBiQ9Y4P9
TreeFamiTF323506

Miscellaneous databases

EvolutionaryTraceiQ9Y4P9
GeneWikiiSPEF1
GenomeRNAii25876
PROiPR:Q9Y4P9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101222 Expressed in 124 organ(s), highest expression level in right uterine tube
CleanExiHS_SPEF1
GenevisibleiQ9Y4P9 HS

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR010441 CH_2
IPR036872 CH_dom_sf
PfamiView protein in Pfam
PF06294 CH_2, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPEF1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y4P9
Secondary accession number(s): A5YM71, D3DVY0, Q5JX78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 10, 2007
Last modified: November 7, 2018
This is version 143 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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