ATG4B

Functionⁱ

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.6 Publications

Activity regulationⁱ

Inhibited by N-ethylmaleimide. Redox-regulated during autophagy since reducing conditions activate ATG4A whereas an oxidizing environment such as the presence of H₂O₂ inhibits its activity.1 Publication

Kineticsⁱ

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	74	Nucleophile	1
Active siteⁱ	278	Sequence analysis	1
Active siteⁱ	280		1

GO - Molecular functionⁱ

cysteine-type endopeptidase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
cysteine-type peptidase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 15169837
endopeptidase activity
Source: BHF-UCL
Inferred from direct assayⁱ
- 18387192

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

autophagosome assembly
Source: UniProtKB
Inferred from genetic interactionⁱ
- 12446702
autophagy
Source: BHF-UCL
Inferred from direct assayⁱ
- 18387192
macroautophagy Source: Reactome
positive regulation of protein catabolic process Source: Ensembl
protein delipidation
Source: MGI
Inferred from direct assayⁱ
- 25327288
protein transport Source: UniProtKB-KW
proteolysis
Source: UniProtKB
Inferred from direct assayⁱ
- 15169837

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Thiol protease
Biological process	Autophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1632852 Macroautophagy
SIGNORⁱ	Q9Y4P1

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	C54.003
TCDBⁱ	9.A.15.2.1 the autophagy-related phagophore-formation transporter (apt) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cysteine protease ATG4B (EC:3.4.22.-) Alternative name(s): AUT-like 1 cysteine endopeptidase Autophagin-1 Autophagy-related cysteine endopeptidase 1 Autophagy-related protein 4 homolog B Short name: hAPG4B
Gene namesⁱ	Name:ATG4B Synonyms:APG4B, AUTL1, KIAA0943
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 2

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000168397.16
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:20790 ATG4B
MIMⁱ	611338 gene
neXtProtⁱ	NX_Q9Y4P1

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	74	C → S: Complete loss of protease activity. 4 Publications	1
Mutagenesisⁱ	78	C → A: Reduces the redox sensitivity and retains activity in presence of H(2)O(2). 1 Publication	1
Mutagenesisⁱ	142	W → A: Strongly reduced protease activity. 1 Publication	1
Mutagenesisⁱ	229	R → A: Strongly reduced protease activity. 1 Publication	1
Mutagenesisⁱ	278	D → A: Complete loss of protease activity. 2 Publications	1
Mutagenesisⁱ	280	H → A: Complete loss of protease activity. 2 Publications	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	23192
Open Targets More...OpenTargetsⁱ	ENSG00000168397
PharmGKBⁱ	PA134898340

Chemistry databases

ChEMBLⁱ	CHEMBL1741221

ChEMBLⁱ

CHEMBL1741221

Polymorphism and mutation databases

BioMutaⁱ	ATG4B
DMDMⁱ	296434400

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000215844	1 – 393	Cysteine protease ATG4BAdd BLAST		393

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	1	N-acetylmethionineCombined sources1 Publication		1
Modified residueⁱ	383	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	Q9Y4P1
PaxDbⁱ	Q9Y4P1
PeptideAtlas More...PeptideAtlasⁱ	Q9Y4P1
PRIDEⁱ	Q9Y4P1
ProteomicsDBⁱ	86230 86231 [Q9Y4P1-2] 86232 [Q9Y4P1-3] 86233 [Q9Y4P1-4] 86234 [Q9Y4P1-6]

PTM databases

iPTMnetⁱ	Q9Y4P1
PhosphoSitePlusⁱ	Q9Y4P1

Expressionⁱ

Tissue specificityⁱ

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000168397 Expressed in 224 organ(s), highest expression level in left lobe of thyroid gland
CleanExⁱ	HS_ATG4B
ExpressionAtlasⁱ	Q9Y4P1 baseline and differential
Genevisibleⁱ	Q9Y4P1 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB037195 HPA069803

HPAⁱ

CAB037195
HPA069803

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
GABARAP	O95166	8	EBI-712014,EBI-712001
GABARAPL1	Q9H0R8	9	EBI-712014,EBI-746969
GABARAPL2	P60520	9	EBI-712014,EBI-720116
MAP1LC3A	Q9H492	5	EBI-712014,EBI-720768
MAP1LC3B	Q9GZQ8	13	EBI-712014,EBI-373144
MAP1LC3C	Q9BXW4	3	EBI-712014,EBI-2603996

Protein-protein interaction databases

BioGridⁱ	116801, 28 interactors
IntActⁱ	Q9Y4P1, 18 interactors
Molecular INTeraction database More...MINTⁱ	Q9Y4P1
STRINGⁱ	9606.ENSP00000384259

Chemistry databases

BindingDBⁱ

Q9Y4P1

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	5 – 7	Combined sources	3
Helixⁱ	10 – 13	Combined sources	4
Helixⁱ	14 – 18	Combined sources	5
Beta strandⁱ	22 – 24	Combined sources	3
Beta strandⁱ	26 – 28	Combined sources	3
Beta strandⁱ	31 – 33	Combined sources	3
Turnⁱ	35 – 37	Combined sources	3
Helixⁱ	39 – 48	Combined sources	10
Beta strandⁱ	54 – 57	Combined sources	4
Turnⁱ	61 – 64	Combined sources	4
Turnⁱ	70 – 72	Combined sources	3
Helixⁱ	74 – 91	Combined sources	18
Beta strandⁱ	100 – 102	Combined sources	3
Helixⁱ	106 – 113	Combined sources	8
Beta strandⁱ	116 – 118	Combined sources	3
Helixⁱ	125 – 133	Combined sources	9
Turnⁱ	134 – 136	Combined sources	3
Helixⁱ	145 – 156	Combined sources	12
Turnⁱ	160 – 162	Combined sources	3
Beta strandⁱ	165 – 168	Combined sources	4
Beta strandⁱ	173 – 175	Combined sources	3
Helixⁱ	176 – 183	Combined sources	8
Beta strandⁱ	184 – 186	Combined sources	3
Beta strandⁱ	222 – 229	Combined sources	8
Beta strandⁱ	232 – 234	Combined sources	3
Helixⁱ	237 – 239	Combined sources	3
Helixⁱ	240 – 246	Combined sources	7
Beta strandⁱ	252 – 257	Combined sources	6
Beta strandⁱ	264 – 270	Combined sources	7
Beta strandⁱ	273 – 277	Combined sources	5
Beta strandⁱ	281 – 284	Combined sources	4
Beta strandⁱ	290 – 292	Combined sources	3
Helixⁱ	297 – 299	Combined sources	3
Beta strandⁱ	306 – 309	Combined sources	4
Helixⁱ	310 – 312	Combined sources	3
Beta strandⁱ	315 – 325	Combined sources	11
Helixⁱ	326 – 341	Combined sources	16
Beta strandⁱ	349 – 353	Combined sources	5
Helixⁱ	371 – 376	Combined sources	6
Beta strandⁱ	388 – 390	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9Y4P1
SMRⁱ	Q9Y4P1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9Y4P1

EvolutionaryTraceⁱ

Q9Y4P1

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the peptidase C54 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG2674 Eukaryota ENOG410XPQ0 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063000
HOVERGENⁱ	HBG050536
InParanoidⁱ	Q9Y4P1
KEGG Orthology (KO) More...KOⁱ	K08342
OMAⁱ	RAPYSIH
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0995
PhylomeDBⁱ	Q9Y4P1
TreeFamⁱ	TF314847

Family and domain databases

InterProⁱ	View protein in InterPro IPR032916 ATG4B_met IPR038765 Papain_like_cys_pep_sf IPR005078 Peptidase_C54
PANTHERⁱ	PTHR22624 PTHR22624, 1 hit PTHR22624:SF39 PTHR22624:SF39, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03416 Peptidase_C54, 1 hit
SUPFAMⁱ	SSF54001 SSF54001, 1 hit

Sequences (5+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 5 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 5 described isoforms and 8 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q9Y4P1-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL 
        60         70         80         90        100
WFTYRKNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ 
       110        120        130        140        150
RKRQPDSYFS VLNAFIDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ 
       160        170        180        190        200
VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRTSVPCA GATAFPADSD 
       210        220        230        240        250
RHCNGFPAGA EVTNRPSPWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP 
       260        270        280        290        300
QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTD GCFIPDESFH 
       310        320        330        340        350
CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE 
       360        370        380        390 
LVELQPSHLA CPDVLNLSLD SSDVERLERF FDSEDEDFEI LSL

Length:393

Mass (Da):44,294

Last modified:May 18, 2010 - v2

Checksum:ⁱF4ADB3192176E0E5

GO

Isoform 2 (identifier: Q9Y4P1-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-1: M → MAHSVPSDSR...GSVGGRTGKM
370-393: DSSDVERLERFFDSEDEDFEILSL → GESCQVQILLM

« Hide

        10         20         30         40         50
MAHSVPSDSR TSRRPTTRPH AARGAPRGSR RPGRTPKWRL PRISARAPYR 
        60         70         80         90        100
LRRLRRHTYW PPRRPVAASR CWPVGATPLG SVGGRTGKMD AATLTYDTLR 
       110        120        130        140        150
FAEFEDFPET SEPVWILGRK YSIFTEKDEI LSDVASRLWF TYRKNFPAIG 
       160        170        180        190        200
GTGPTSDTGW GCMLRCGQMI FAQALVCRHL GRDWRWTQRK RQPDSYFSVL 
       210        220        230        240        250
NAFIDRKDSY YSIHQIAQMG VGEGKSIGQW YGPNTVAQVL KKLAVFDTWS 
       260        270        280        290        300
SLAVHIAMDN TVVMEEIRRL CRTSVPCAGA TAFPADSDRH CNGFPAGAEV 
       310        320        330        340        350
TNRPSPWRPL VLLIPLRLGL TDINEAYVET LKHCFMMPQS LGVIGGKPNS 
       360        370        380        390        400
AHYFIGYVGE ELIYLDPHTT QPAVEPTDGC FIPDESFHCQ HPPCRMSIAE 
       410        420        430        440        450
LDPSIAVGFF CKTEDDFNDW CQQVKKLSLL GGALPMFELV ELQPSHLACP 
       460 
DVLNLSLGES CQVQILLM

Note: No experimental confirmation available.

Show »

Length:468

Mass (Da):52,529

Checksum:ⁱF9731E7C406960D6

GO

Isoform 3 (identifier: Q9Y4P1-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.
     321-354: FFCKTEDDFNDWCQQVKKLSLLGGALPMFELVEL → KQGRLVRSLIPWAPRPSSWCAAVLGAAVVMCGTP
     355-393: Missing.

« Hide

        10         20         30         40         50
MLRCGQMIFA QALVCRHLGR DWRWTQRKRQ PDSYFSVLNA FIDRKDSYYS 
        60         70         80         90        100
IHQIAQMGVG EGKSIGQWYG PNTVAQVLKK LAVFDTWSSL AVHIAMDNTV 
       110        120        130        140        150
VMEEIRRLCR TSVPCAGATA FPADSDRHCN GFPAGAEVTN RPSPWRPLVL 
       160        170        180        190        200
LIPLRLGLTD INEAYVETLK HCFMMPQSLG VIGGKPNSAH YFIGYVGEEL 
       210        220        230        240        250
IYLDPHTTQP AVEPTDGCFI PDESFHCQHP PCRMSIAELD PSIAVGKQGR 
       260        270        280
LVRSLIPWAP RPSSWCAAVL GAAVVMCGTP

Note: No experimental confirmation available.

Show »

Length:280

Mass (Da):31,042

Checksum:ⁱ0904A795A1B596B3

GO

Isoform 4 (identifier: Q9Y4P1-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-74: Missing.
369-369: L → LGESCQVQVGSLG

« Hide

        10         20         30         40         50
MLRCGQMIFA QALVCRHLGR DWRWTQRKRQ PDSYFSVLNA FIDRKDSYYS 
        60         70         80         90        100
IHQIAQMGVG EGKSIGQWYG PNTVAQVLKK LAVFDTWSSL AVHIAMDNTV 
       110        120        130        140        150
VMEEIRRLCR TSVPCAGATA FPADSDRHCN GFPAGAEVTN RPSPWRPLVL 
       160        170        180        190        200
LIPLRLGLTD INEAYVETLK HCFMMPQSLG VIGGKPNSAH YFIGYVGEEL 
       210        220        230        240        250
IYLDPHTTQP AVEPTDGCFI PDESFHCQHP PCRMSIAELD PSIAVGFFCK 
       260        270        280        290        300
TEDDFNDWCQ QVKKLSLLGG ALPMFELVEL QPSHLACPDV LNLSLGESCQ 
       310        320        330 
VQVGSLGDSS DVERLERFFD SEDEDFEILS L

Show »

Length:331

Mass (Da):37,037

Checksum:ⁱ97592E25838601B2

GO

Isoform 6 (identifier: Q9Y4P1-6) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
370-393: DSSDVERLERFFDSEDEDFEILSL → GESCQVQILLM

« Hide

        10         20         30         40         50
MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL 
        60         70         80         90        100
WFTYRKNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ 
       110        120        130        140        150
RKRQPDSYFS VLNAFIDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ 
       160        170        180        190        200
VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRTSVPCA GATAFPADSD 
       210        220        230        240        250
RHCNGFPAGA EVTNRPSPWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP 
       260        270        280        290        300
QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTD GCFIPDESFH 
       310        320        330        340        350
CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE 
       360        370        380
LVELQPSHLA CPDVLNLSLG ESCQVQILLM

Note: No experimental confirmation available.

Show »

Length:380

Mass (Da):42,622

Checksum:ⁱ48140873BCE7533D

GO

Computationally mapped potential isoform sequencesⁱ

There are 8 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

C9JQ72	C9JQ72_HUMAN	Cysteine protease Cysteine protease, EC 3.4.22.-	ATG4B	266	Annotation score:
H7C2A6	H7C2A6_HUMAN	Cysteine protease Cysteine protease, EC 3.4.22.-	ATG4B	193	Annotation score:
C9JIK8	C9JIK8_HUMAN	Cysteine protease Cysteine protease, EC 3.4.22.-	ATG4B	219	Annotation score:
C9J3C0	C9J3C0_HUMAN	Cysteine protease Cysteine protease, EC 3.4.22.-	ATG4B	147	Annotation score:
C9J1C1	C9J1C1_HUMAN	Cysteine protease Cysteine protease, EC 3.4.22.-	ATG4B	66	Annotation score:
C9JVD5	C9JVD5_HUMAN	Cysteine protease Cysteine protease, EC 3.4.22.-	ATG4B	105	Annotation score:
H0Y2Y0	H0Y2Y0_HUMAN	Cysteine protease ATG4B Cysteine protease ATG4B	ATG4B	76	Annotation score:
F2Z2K8	F2Z2K8_HUMAN	Cysteine protease ATG4B Cysteine protease ATG4B	ATG4B	88	Annotation score:

Sequence cautionⁱ

The sequence BAA76787 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

The sequence BAC86110 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	136	Missing in BAB83890 (PubMed:15169837).Curated	1
Sequence conflictⁱ	188	P → L in BAB55127 (PubMed:14702039).Curated	1
Sequence conflictⁱ	247	F → Y in BAB55353 (PubMed:14702039).Curated	1
Sequence conflictⁱ	273	E → G in BAB55042 (PubMed:14702039).Curated	1
Sequence conflictⁱ	312	E → N in BAB83890 (PubMed:15169837).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_021486	354	L → Q5 PublicationsCorresponds to variant dbSNP:rs7601000Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_013028	1 – 74	Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST	74
Alternative sequenceⁱVSP_013029	1	M → MAHSVPSDSRTSRRPTTRPH AARGAPRGSRRPGRTPKWRL PRISARAPYRLRRLRRHTYW PPRRPVAASRCWPVGATPLG SVGGRTGKM in isoform 2. 1 Publication	1
Alternative sequenceⁱVSP_013031	321 – 354	FFCKT…ELVEL → KQGRLVRSLIPWAPRPSSWC AAVLGAAVVMCGTP in isoform 3. 1 PublicationAdd BLAST	34
Alternative sequenceⁱVSP_013032	355 – 393	Missing in isoform 3. 1 PublicationAdd BLAST	39
Alternative sequenceⁱVSP_013033	369	L → LGESCQVQVGSLG in isoform 4. 1 Publication	1
Alternative sequenceⁱVSP_013034	370 – 393	DSSDV…EILSL → GESCQVQILLM in isoform 2 and isoform 6. 1 PublicationAdd BLAST	24

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ504652 mRNA Translation: CAD43219.1 AB066215 mRNA Translation: BAB83890.1 AB023160 mRNA Translation: BAA76787.2 Different initiation. AK027332 mRNA Translation: BAB55042.1 AK027462 mRNA Translation: BAB55127.1 AK027763 mRNA Translation: BAB55353.1 AK125277 mRNA Translation: BAC86110.1 Different initiation. AL080168 mRNA Translation: CAB45756.1 AC133528 Genomic DNA Translation: AAY14919.1 BC000719 mRNA Translation: AAH00719.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS46564.1 [Q9Y4P1-1] CCDS46565.1 [Q9Y4P1-6]
PIRⁱ	T12492
NCBI Reference Sequences More...RefSeqⁱ	NP_037457.3, NM_013325.4 [Q9Y4P1-1] NP_847896.1, NM_178326.2 [Q9Y4P1-6]
UniGeneⁱ	Hs.283610

Genome annotation databases

Ensemblⁱ	ENST00000402096; ENSP00000384661; ENSG00000168397 [Q9Y4P1-4] ENST00000404914; ENSP00000384259; ENSG00000168397 [Q9Y4P1-1] ENST00000405546; ENSP00000383964; ENSG00000168397 [Q9Y4P1-6]
GeneIDⁱ	23192
KEGGⁱ	hsa:23192
UCSC genome browser More...UCSCⁱ	uc002wbv.4 human [Q9Y4P1-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9Y4P1	Cysteine protease ATG4B		MOUSE		393	UniRef90_Q9Y4P1
Cysteine protease		MYOBR		420
cysteine protease ATG4B		URSMA		396
Cysteine protease		CAVPO		395
Cysteine protease (Fragment)		DESRO		394
+61
Q9Y4P1-2	Isoform 6 of Cysteine protease ATG4B		HUMAN		380	UniRef90_Q9Y4P1-2
Cysteine protease		GORGO		263
Cysteine protease (Fragment)		PONAB		105
Cysteine protease (Fragment)		PANTR		105
Cysteine protease		CALJA		481
+26
Q9Y4P1-4	Cysteine protease		MYOLU		500	UniRef90_Q9Y4P1-4
Cysteine protease		NEOSC		406
Cysteine protease		MANLE		403
Cysteine protease		SAIBB		401
Cysteine protease		MACMU		401
+20
Q9Y4P1-6	Isoform 2 of Cysteine protease ATG4B		HUMAN		468	UniRef90_Q9Y4P1-2
Cysteine protease		GORGO		263
Cysteine protease (Fragment)		PONAB		105
Cysteine protease (Fragment)		PANTR		105
Cysteine protease		CALJA		481
+26

Protein	Similar proteins		Species	Score	Length	Source
Q9Y4P1	Cysteine protease ATG4B		MOUSE		393	UniRef50_Q9Y4P1
Cysteine protease		MYOBR		420
cysteine protease ATG4B		URSMA		396
Cysteine protease		CAVPO		395
Cysteine protease		MYODS		394
+213

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ504652 mRNA Translation: CAD43219.1 AB066215 mRNA Translation: BAB83890.1 AB023160 mRNA Translation: BAA76787.2 Different initiation. AK027332 mRNA Translation: BAB55042.1 AK027462 mRNA Translation: BAB55127.1 AK027763 mRNA Translation: BAB55353.1 AK125277 mRNA Translation: BAC86110.1 Different initiation. AL080168 mRNA Translation: CAB45756.1 AC133528 Genomic DNA Translation: AAY14919.1 BC000719 mRNA Translation: AAH00719.1
CCDSⁱ	CCDS46564.1 [Q9Y4P1-1] CCDS46565.1 [Q9Y4P1-6]
PIRⁱ	T12492
RefSeqⁱ	NP_037457.3, NM_013325.4 [Q9Y4P1-1] NP_847896.1, NM_178326.2 [Q9Y4P1-6]
UniGeneⁱ	Hs.283610

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2CY7	X-ray	1.90	A	1-393	[»]
	2D1I	X-ray	2.00	A/B	1-393	[»]
	2Z0D	X-ray	1.90	A	1-353	[»]
	2Z0E	X-ray	1.90	A	1-353	[»]
	2ZZP	X-ray	2.05	A	1-353	[»]
	5LXH	X-ray	1.58	E/F/G	384-393	[»]
	5LXI	X-ray	1.44	C/E	384-393	[»]
ProteinModelPortalⁱ	Q9Y4P1
SMRⁱ	Q9Y4P1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	116801, 28 interactors
IntActⁱ	Q9Y4P1, 18 interactors
MINTⁱ	Q9Y4P1
STRINGⁱ	9606.ENSP00000384259

Chemistry databases

BindingDBⁱ	Q9Y4P1
ChEMBLⁱ	CHEMBL1741221

Protein family/group databases

MEROPSⁱ	C54.003
TCDBⁱ	9.A.15.2.1 the autophagy-related phagophore-formation transporter (apt) family

PTM databases

iPTMnetⁱ	Q9Y4P1
PhosphoSitePlusⁱ	Q9Y4P1

Polymorphism and mutation databases

BioMutaⁱ	ATG4B
DMDMⁱ	296434400

Proteomic databases

EPDⁱ	Q9Y4P1
PaxDbⁱ	Q9Y4P1
PeptideAtlasⁱ	Q9Y4P1
PRIDEⁱ	Q9Y4P1
ProteomicsDBⁱ	86230 86231 [Q9Y4P1-2] 86232 [Q9Y4P1-3] 86233 [Q9Y4P1-4] 86234 [Q9Y4P1-6]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	23192
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000402096; ENSP00000384661; ENSG00000168397 [Q9Y4P1-4] ENST00000404914; ENSP00000384259; ENSG00000168397 [Q9Y4P1-1] ENST00000405546; ENSP00000383964; ENSG00000168397 [Q9Y4P1-6]
GeneIDⁱ	23192
KEGGⁱ	hsa:23192
UCSCⁱ	uc002wbv.4 human [Q9Y4P1-1]

Organism-specific databases

CTDⁱ	23192
DisGeNETⁱ	23192
EuPathDBⁱ	HostDB:ENSG00000168397.16
GeneCardsⁱ	ATG4B
H-InvDBⁱ	HIX0180188
HGNCⁱ	HGNC:20790 ATG4B
HPAⁱ	CAB037195 HPA069803
MIMⁱ	611338 gene
neXtProtⁱ	NX_Q9Y4P1
OpenTargetsⁱ	ENSG00000168397
PharmGKBⁱ	PA134898340
HUGEⁱ	Search...
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2674 Eukaryota ENOG410XPQ0 LUCA
GeneTreeⁱ	ENSGT00530000063000
HOVERGENⁱ	HBG050536
InParanoidⁱ	Q9Y4P1
KOⁱ	K08342
OMAⁱ	RAPYSIH
OrthoDBⁱ	EOG091G0995
PhylomeDBⁱ	Q9Y4P1
TreeFamⁱ	TF314847

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1632852 Macroautophagy
SIGNORⁱ	Q9Y4P1

Miscellaneous databases

ChiTaRSⁱ	ATG4B human
EvolutionaryTraceⁱ	Q9Y4P1
GeneWikiⁱ	ATG4B
GenomeRNAiⁱ	23192
Protein Ontology More...PROⁱ	PR:Q9Y4P1
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000168397 Expressed in 224 organ(s), highest expression level in left lobe of thyroid gland
CleanExⁱ	HS_ATG4B
ExpressionAtlasⁱ	Q9Y4P1 baseline and differential
Genevisibleⁱ	Q9Y4P1 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR032916 ATG4B_met IPR038765 Papain_like_cys_pep_sf IPR005078 Peptidase_C54
PANTHERⁱ	PTHR22624 PTHR22624, 1 hit PTHR22624:SF39 PTHR22624:SF39, 1 hit
Pfamⁱ	View protein in Pfam PF03416 Peptidase_C54, 1 hit
SUPFAMⁱ	SSF54001 SSF54001, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ATG4B_HUMAN
Accessionⁱ	Q9Y4P1Primary (citable) accession number: Q9Y4P1 Secondary accession number(s): B7WNK2 Q9Y2F2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
	Last sequence update:	May 18, 2010
	Last modified:	November 7, 2018
	This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Peptidase families
Classification of peptidase families and list of entries
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9Y4P1 (ATG4B_HUMAN)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Cysteine protease ATG4B

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

Kineticsⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ