UniProtKB - Q9Y4P1 (ATG4B_HUMAN)
Cysteine protease ATG4B
ATG4B
Functioni
Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins (PubMed:15169837, PubMed:15187094, PubMed:17347651, PubMed:19322194, PubMed:21177865, PubMed:26378241, PubMed:29232556, PubMed:28821708, PubMed:30443548, PubMed:30661429, PubMed:22302004, PubMed:27527864, PubMed:28633005, PubMed:30076329).
Required for canonical autophagy (macroautophagy), non-canonical autophagy as well as for mitophagy (PubMed:33773106, PubMed:33909989).
The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine (PubMed:15169837, PubMed:15187094, PubMed:17347651, PubMed:20818167, PubMed:19322194, PubMed:21177865, PubMed:22302004, PubMed:27527864, PubMed:28633005, PubMed:29458288, PubMed:30661429, PubMed:28287329).
Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (PubMed:15169837, PubMed:15187094, PubMed:17347651, PubMed:19322194, PubMed:21177865, PubMed:22302004).
Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3 (PubMed:31315929, PubMed:33773106).
In addition to the protease activity, also mediates delipidation of ATG8 family proteins (PubMed:15187094, PubMed:28633005, PubMed:29458288, PubMed:32686895, PubMed:33909989, PubMed:19322194).
Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy (PubMed:15187094, PubMed:29458288, PubMed:32686895, PubMed:33909989, PubMed:19322194).
Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, by catalyzing delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989).
Compared to other members of the family (ATG4A, ATG4C or ATG4C), constitutes the major protein for proteolytic activation of ATG8 proteins, while it displays weaker delipidation activity than other ATG4 paralogs (PubMed:29458288, PubMed:30661429).
Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins: acts by regulating ATG9A trafficking to mitochondria and promoting phagophore-endoplasmic reticulum contacts during the lipid transfer phase of mitophagy (PubMed:33773106).
21 PublicationsCaution
Catalytic activityi
- [protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine5 PublicationsThis reaction proceeds in the forward5 Publications direction.
- [protein]-C-terminal L-amino acid-glycyl-phosphatidylserine + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phospho-L-serine1 PublicationThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Kineticsi
- KM=5.1 µM for MAP1LC3B2 Publications
- KM=5.8 µM for GABARAP2 Publications
- KM=4.4 µM for GABARAPL12 Publications
- KM=6.1 µM for GABARAPL22 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 74 | Nucleophile8 Publications | 1 | |
Active sitei | 278 | Sequence analysis2 Publications | 1 | |
Active sitei | 280 | 2 Publications | 1 |
GO - Molecular functioni
- Atg8-specific protease activity Source: UniProtKB
- cysteine-type endopeptidase activity Source: Reactome
- cysteine-type peptidase activity Source: UniProtKB
- endopeptidase activity Source: BHF-UCL
- scaffold protein binding Source: ARUK-UCL
GO - Biological processi
- autophagy Source: UniProtKB
- lysosomal microautophagy Source: UniProtKB
- macroautophagy Source: Reactome
- mitophagy Source: UniProtKB
- otolith mineralization completed early in development Source: UniProtKB
- protein delipidation Source: UniProtKB
- protein localization to phagophore assembly site Source: UniProtKB
- protein transport Source: UniProtKB-KW
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Autophagy, Protein transport, Transport, Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | Q9Y4P1 |
Reactomei | R-HSA-1632852, Macroautophagy |
SABIO-RKi | Q9Y4P1 |
SignaLinki | Q9Y4P1 |
SIGNORi | Q9Y4P1 |
Protein family/group databases
MEROPSi | C54.003 |
TCDBi | 9.A.15.2.1, the autophagy-related phagophore-formation transporter (apt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Cysteine protease ATG4BCurated (EC:3.4.22.-2 Publications)Alternative name(s): AUT-like 1 cysteine endopeptidase Autophagy-related cysteine endopeptidase 11 Publication Short name: Autophagin-11 Publication Autophagy-related protein 4 homolog B1 Publication Short name: HsAPG4B1 Publication Short name: hAPG4B1 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20790, ATG4B |
MIMi | 611338, gene |
neXtProti | NX_Q9Y4P1 |
VEuPathDBi | HostDB:ENSG00000168397 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Cytoplasm and Cytosol
Other locations
- autophagosome 1 Publication
Note: Mainly localizes to the cytoplasm, including cytosol (PubMed:29165041). A samll potion localizes to mitochondria; phosphorylation at Ser-34 promotes localization to mitochondria (PubMed:29165041).1 Publication
Cytosol
- cytosol Source: Reactome
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 34 | S → A: Decreased phosphorylation by AKT2, leading to reduced proteolytic activation of ATG8 family proteins. 1 Publication | 1 | |
Mutagenesisi | 34 | S → D: Phospho-mimetic mutant; increased proteolytic activation of ATG8 family proteins. 1 Publication | 1 | |
Mutagenesisi | 74 | C → S: Complete loss of protease activity. 8 Publications | 1 | |
Mutagenesisi | 78 | C → A: Reduces the redox sensitivity and retains activity in presence of H(2)O(2). 1 Publication | 1 | |
Mutagenesisi | 78 | C → S: Does not affect formation of disulfide bonds. 1 Publication | 1 | |
Mutagenesisi | 89 | C → S: Does not affect formation of disulfide bonds. 1 Publication | 1 | |
Mutagenesisi | 121 | S → A: Decreased phosphorylation by AKT2; when associated with A-262. 1 Publication | 1 | |
Mutagenesisi | 142 | W → A: Strongly reduced protease activity. 1 Publication | 1 | |
Mutagenesisi | 183 | C → S: Does not affect formation of disulfide bonds. 1 Publication | 1 | |
Mutagenesisi | 189 | C → S: Does not affect S-nitrosylation. Strongly decreased S-nitrosylation, leading to increased hydrolase activity and autophagic flux; when associated with S-292. Does not affect formation of disulfide bonds. 2 Publications | 1 | |
Mutagenesisi | 203 | C → S: Does not affect formation of disulfide bonds. 1 Publication | 1 | |
Mutagenesisi | 229 | R → A: Strongly reduced protease activity. 1 Publication | 1 | |
Mutagenesisi | 246 | C → S: Does not affect formation of disulfide bonds. 1 Publication | 1 | |
Mutagenesisi | 262 | S → A: Decreased phosphorylation by AKT2; when associated with A-121. 1 Publication | 1 | |
Mutagenesisi | 278 | D → A: Complete loss of protease activity. 2 Publications | 1 | |
Mutagenesisi | 280 | H → A: Complete loss of protease activity. 2 Publications | 1 | |
Mutagenesisi | 292 | C → S: Does not affect S-nitrosylation. Strongly decreased S-nitrosylation, leading to increased hydrolase activity and autophagic flux; when associated with S-189. Reduced formation of intrachain and interchain disulfide bonds in response to oxidation. Abolished formation of disulfide bonds, leading to increased autophagy; when associated with S-361. 2 Publications | 1 | |
Mutagenesisi | 301 | C → S: Does not affect S-nitrosylation. Does not affect formation of disulfide bonds. 2 Publications | 1 | |
Mutagenesisi | 306 | C → S: Does not affect formation of disulfide bonds. 1 Publication | 1 | |
Mutagenesisi | 316 | S → A: Abolished phosphorylation by ULK1; promotes hydrolase activity, leading to increased proteolytic activation and delipidation of ATG8 family proteins. 1 Publication | 1 | |
Mutagenesisi | 316 | S → D: Phospho-mimetic mutant; reduced hydrolase activity, leading to decreased proteolytic activation and delipidation of ATG8 family proteins. 1 Publication | 1 | |
Mutagenesisi | 323 | C → S: Does not affect formation of disulfide bonds. 1 Publication | 1 | |
Mutagenesisi | 333 | C → S: Does not affect formation of disulfide bonds. 1 Publication | 1 | |
Mutagenesisi | 361 | C → S: Reduced formation of intrachain and interchain disulfide bonds in response to oxidation. Abolished formation of disulfide bonds, leading to increased autophagy; when associated with S-292. 1 Publication | 1 | |
Mutagenesisi | 383 | S → A: Decreased phosphorylation, leading to decreased hydrolase activity and autophagic flux. Does not affect interaction with ATG8 family proteins. 3 Publications | 1 | |
Mutagenesisi | 383 | S → E: Phospho-mimetic mutant; does not affect interaction with ATG8 family proteins. 1 Publication | 1 | |
Mutagenesisi | 388 – 391 | FEIL → AEIA in 2mLIR; decreased interaction with ATG8 family proteins MAP1LC3A, MAP1LC3B and MAP1LC3C. Decreased ability to mediate delipidation of ATG8 proteins conjugated to phosphatidylethanolamine (PE). 2 Publications | 4 | |
Mutagenesisi | 392 | S → A: Decreased phosphorylation, leading to decreased hydrolase activity and autophagic flux. Does not affect interaction with ATG8 family proteins. 2 Publications | 1 | |
Mutagenesisi | 392 | S → E: Phospho-mimetic mutant; slightly increased interaction with ATG8 family proteins. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 23192 |
OpenTargetsi | ENSG00000168397 |
PharmGKBi | PA134898340 |
Miscellaneous databases
Pharosi | Q9Y4P1, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1741221 |
Genetic variation databases
BioMutai | ATG4B |
DMDMi | 296434400 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215844 | 1 – 393 | Cysteine protease ATG4BAdd BLAST | 393 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources1 Publication | 1 | |
Modified residuei | 34 | Phosphoserine; by PKB/AKT1 and PKB/AKT23 Publications | 1 | |
Modified residuei | 189 | S-nitrosocysteine1 Publication | 1 | |
Disulfide bondi | 292 ↔ 361 | 1 Publication | ||
Modified residuei | 292 | S-nitrosocysteine1 Publication | 1 | |
Disulfide bondi | 292 | Interchain (with C-361)1 Publication | ||
Modified residuei | 301 | S-nitrosocysteine1 Publication | 1 | |
Modified residuei | 316 | Phosphoserine; by ULK11 Publication | 1 | |
Disulfide bondi | 361 | Interchain (with C-292)1 Publication | ||
Modified residuei | 383 | Phosphoserine; by STK26Combined sources2 Publications | 1 | |
Modified residuei | 392 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Disulfide bond, Glycoprotein, Isopeptide bond, Phosphoprotein, S-nitrosylation, Ubl conjugationProteomic databases
EPDi | Q9Y4P1 |
jPOSTi | Q9Y4P1 |
MassIVEi | Q9Y4P1 |
MaxQBi | Q9Y4P1 |
PaxDbi | Q9Y4P1 |
PeptideAtlasi | Q9Y4P1 |
PRIDEi | Q9Y4P1 |
ProteomicsDBi | 86230 [Q9Y4P1-1] 86231 [Q9Y4P1-2] 86232 [Q9Y4P1-3] 86233 [Q9Y4P1-4] 86234 [Q9Y4P1-6] |
PTM databases
GlyGeni | Q9Y4P1, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9Y4P1 |
PhosphoSitePlusi | Q9Y4P1 |
Expressioni
Gene expression databases
Bgeei | ENSG00000168397, Expressed in left lobe of thyroid gland and 237 other tissues |
ExpressionAtlasi | Q9Y4P1, baseline and differential |
Genevisiblei | Q9Y4P1, HS |
Organism-specific databases
HPAi | ENSG00000168397, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with PFKP; promoting phosphorylation of ATG4B at Ser-34 (PubMed:33607258).
Interacts with GBP7 (By similarity).
By similarity1 PublicationBinary interactionsi
Q9Y4P1
GO - Molecular functioni
- scaffold protein binding Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 116801, 50 interactors |
IntActi | Q9Y4P1, 23 interactors |
MINTi | Q9Y4P1 |
STRINGi | 9606.ENSP00000384259 |
Chemistry databases
BindingDBi | Q9Y4P1 |
Miscellaneous databases
RNActi | Q9Y4P1, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9Y4P1 |
SMRi | Q9Y4P1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9Y4P1 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 388 – 391 | LIR1 Publication | 4 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2674, Eukaryota |
GeneTreei | ENSGT00530000063000 |
HOGENOMi | CLU_021259_0_1_1 |
InParanoidi | Q9Y4P1 |
OMAi | CHTRRIR |
OrthoDBi | 431748at2759 |
PhylomeDBi | Q9Y4P1 |
TreeFami | TF314847 |
Family and domain databases
DisProti | DP01326 |
IDEALi | IID00347 |
InterProi | View protein in InterPro IPR032916, ATG4B_met IPR038765, Papain-like_cys_pep_sf IPR005078, Peptidase_C54 |
PANTHERi | PTHR22624, PTHR22624, 1 hit PTHR22624:SF39, PTHR22624:SF39, 1 hit |
Pfami | View protein in Pfam PF03416, Peptidase_C54, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL
60 70 80 90 100
WFTYRKNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ
110 120 130 140 150
RKRQPDSYFS VLNAFIDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ
160 170 180 190 200
VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRTSVPCA GATAFPADSD
210 220 230 240 250
RHCNGFPAGA EVTNRPSPWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP
260 270 280 290 300
QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTD GCFIPDESFH
310 320 330 340 350
CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE
360 370 380 390
LVELQPSHLA CPDVLNLSLD SSDVERLERF FDSEDEDFEI LSL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J1C1 | C9J1C1_HUMAN | Cysteine protease | ATG4B | 66 | Annotation score: | ||
C9JIK8 | C9JIK8_HUMAN | Cysteine protease | ATG4B | 219 | Annotation score: | ||
H0Y2Y0 | H0Y2Y0_HUMAN | Cysteine protease ATG4B | ATG4B | 76 | Annotation score: | ||
F2Z2K8 | F2Z2K8_HUMAN | Cysteine protease ATG4B | ATG4B | 88 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 136 | Missing in BAB83890 (PubMed:15169837).Curated | 1 | |
Sequence conflicti | 188 | P → L in BAB55127 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 247 | F → Y in BAB55353 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 273 | E → G in BAB55042 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 312 | E → N in BAB83890 (PubMed:15169837).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021486 | 354 | L → Q4 PublicationsCorresponds to variant dbSNP:rs7601000Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013028 | 1 – 74 | Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST | 74 | |
Alternative sequenceiVSP_013029 | 1 | M → MAHSVPSDSRTSRRPTTRPH AARGAPRGSRRPGRTPKWRL PRISARAPYRLRRLRRHTYW PPRRPVAASRCWPVGATPLG SVGGRTGKM in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_013031 | 321 – 354 | FFCKT…ELVEL → KQGRLVRSLIPWAPRPSSWC AAVLGAAVVMCGTP in isoform 3. 1 PublicationAdd BLAST | 34 | |
Alternative sequenceiVSP_013032 | 355 – 393 | Missing in isoform 3. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_013033 | 369 | L → LGESCQVQVGSLG in isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_013034 | 370 – 393 | DSSDV…EILSL → GESCQVQILLM in isoform 2 and isoform 6. 1 PublicationAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ504652 mRNA Translation: CAD43219.1 AB066215 mRNA Translation: BAB83890.1 AB023160 mRNA Translation: BAA76787.2 Different initiation. AK027332 mRNA Translation: BAB55042.1 AK027462 mRNA Translation: BAB55127.1 AK027763 mRNA Translation: BAB55353.1 AK125277 mRNA Translation: BAC86110.1 Different initiation. AL080168 mRNA Translation: CAB45756.1 AC133528 Genomic DNA Translation: AAY14919.1 BC000719 mRNA Translation: AAH00719.1 |
CCDSi | CCDS46564.1 [Q9Y4P1-1] CCDS46565.1 [Q9Y4P1-6] |
PIRi | T12492 |
RefSeqi | NP_037457.3, NM_013325.4 [Q9Y4P1-1] NP_847896.1, NM_178326.2 [Q9Y4P1-6] |
Genome annotation databases
Ensembli | ENST00000402096.5; ENSP00000384661.1; ENSG00000168397.17 [Q9Y4P1-4] ENST00000404914.8; ENSP00000384259.3; ENSG00000168397.17 ENST00000405546.7; ENSP00000383964.3; ENSG00000168397.17 [Q9Y4P1-6] |
GeneIDi | 23192 |
KEGGi | hsa:23192 |
MANE-Selecti | ENST00000404914.8; ENSP00000384259.3; NM_013325.5; NP_037457.3 |
UCSCi | uc002wbv.4, human [Q9Y4P1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ504652 mRNA Translation: CAD43219.1 AB066215 mRNA Translation: BAB83890.1 AB023160 mRNA Translation: BAA76787.2 Different initiation. AK027332 mRNA Translation: BAB55042.1 AK027462 mRNA Translation: BAB55127.1 AK027763 mRNA Translation: BAB55353.1 AK125277 mRNA Translation: BAC86110.1 Different initiation. AL080168 mRNA Translation: CAB45756.1 AC133528 Genomic DNA Translation: AAY14919.1 BC000719 mRNA Translation: AAH00719.1 |
CCDSi | CCDS46564.1 [Q9Y4P1-1] CCDS46565.1 [Q9Y4P1-6] |
PIRi | T12492 |
RefSeqi | NP_037457.3, NM_013325.4 [Q9Y4P1-1] NP_847896.1, NM_178326.2 [Q9Y4P1-6] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CY7 | X-ray | 1.90 | A | 1-393 | [»] | |
2D1I | X-ray | 2.00 | A/B | 1-393 | [»] | |
2Z0D | X-ray | 1.90 | A | 1-353 | [»] | |
2Z0E | X-ray | 1.90 | A | 1-353 | [»] | |
2ZZP | X-ray | 2.05 | A | 1-353 | [»] | |
5LXH | X-ray | 1.58 | E/F/G | 384-393 | [»] | |
5LXI | X-ray | 1.44 | C/E | 384-393 | [»] | |
AlphaFoldDBi | Q9Y4P1 | |||||
SMRi | Q9Y4P1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116801, 50 interactors |
IntActi | Q9Y4P1, 23 interactors |
MINTi | Q9Y4P1 |
STRINGi | 9606.ENSP00000384259 |
Chemistry databases
BindingDBi | Q9Y4P1 |
ChEMBLi | CHEMBL1741221 |
Protein family/group databases
MEROPSi | C54.003 |
TCDBi | 9.A.15.2.1, the autophagy-related phagophore-formation transporter (apt) family |
PTM databases
GlyGeni | Q9Y4P1, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9Y4P1 |
PhosphoSitePlusi | Q9Y4P1 |
Genetic variation databases
BioMutai | ATG4B |
DMDMi | 296434400 |
Proteomic databases
EPDi | Q9Y4P1 |
jPOSTi | Q9Y4P1 |
MassIVEi | Q9Y4P1 |
MaxQBi | Q9Y4P1 |
PaxDbi | Q9Y4P1 |
PeptideAtlasi | Q9Y4P1 |
PRIDEi | Q9Y4P1 |
ProteomicsDBi | 86230 [Q9Y4P1-1] 86231 [Q9Y4P1-2] 86232 [Q9Y4P1-3] 86233 [Q9Y4P1-4] 86234 [Q9Y4P1-6] |
Protocols and materials databases
Antibodypediai | 12105, 716 antibodies from 41 providers |
DNASUi | 23192 |
Genome annotation databases
Ensembli | ENST00000402096.5; ENSP00000384661.1; ENSG00000168397.17 [Q9Y4P1-4] ENST00000404914.8; ENSP00000384259.3; ENSG00000168397.17 ENST00000405546.7; ENSP00000383964.3; ENSG00000168397.17 [Q9Y4P1-6] |
GeneIDi | 23192 |
KEGGi | hsa:23192 |
MANE-Selecti | ENST00000404914.8; ENSP00000384259.3; NM_013325.5; NP_037457.3 |
UCSCi | uc002wbv.4, human [Q9Y4P1-1] |
Organism-specific databases
CTDi | 23192 |
DisGeNETi | 23192 |
GeneCardsi | ATG4B |
HGNCi | HGNC:20790, ATG4B |
HPAi | ENSG00000168397, Low tissue specificity |
MIMi | 611338, gene |
neXtProti | NX_Q9Y4P1 |
OpenTargetsi | ENSG00000168397 |
PharmGKBi | PA134898340 |
VEuPathDBi | HostDB:ENSG00000168397 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2674, Eukaryota |
GeneTreei | ENSGT00530000063000 |
HOGENOMi | CLU_021259_0_1_1 |
InParanoidi | Q9Y4P1 |
OMAi | CHTRRIR |
OrthoDBi | 431748at2759 |
PhylomeDBi | Q9Y4P1 |
TreeFami | TF314847 |
Enzyme and pathway databases
PathwayCommonsi | Q9Y4P1 |
Reactomei | R-HSA-1632852, Macroautophagy |
SABIO-RKi | Q9Y4P1 |
SignaLinki | Q9Y4P1 |
SIGNORi | Q9Y4P1 |
Miscellaneous databases
BioGRID-ORCSi | 23192, 14 hits in 1088 CRISPR screens |
ChiTaRSi | ATG4B, human |
EvolutionaryTracei | Q9Y4P1 |
GeneWikii | ATG4B |
GenomeRNAii | 23192 |
Pharosi | Q9Y4P1, Tchem |
PROi | PR:Q9Y4P1 |
RNActi | Q9Y4P1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000168397, Expressed in left lobe of thyroid gland and 237 other tissues |
ExpressionAtlasi | Q9Y4P1, baseline and differential |
Genevisiblei | Q9Y4P1, HS |
Family and domain databases
DisProti | DP01326 |
IDEALi | IID00347 |
InterProi | View protein in InterPro IPR032916, ATG4B_met IPR038765, Papain-like_cys_pep_sf IPR005078, Peptidase_C54 |
PANTHERi | PTHR22624, PTHR22624, 1 hit PTHR22624:SF39, PTHR22624:SF39, 1 hit |
Pfami | View protein in Pfam PF03416, Peptidase_C54, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATG4B_HUMAN | |
Accessioni | Q9Y4P1Primary (citable) accession number: Q9Y4P1 Secondary accession number(s): B7WNK2 Q9Y2F2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | May 18, 2010 | |
Last modified: | May 25, 2022 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families