RNF115

Functionⁱ

E3 ubiquitin-protein ligase that mediates E2-dependent, 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of substrates and may play a role in diverse biological processes. Through their polyubiquitination, may play a role in the endosomal trafficking and degradation of membrane receptors including EGFR, FLT3, MET and CXCR4.1 Publication2 Publications

Catalytic activityⁱ

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	228 – 269	RING-typePROSITE-ProRule annotationAdd BLAST		42

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW
ubiquitin protein ligase activity Source: UniProtKB
ubiquitin-protein transferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 18819927

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of epidermal growth factor receptor signaling pathway
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23418353
protein autoubiquitination
Source: UniProtKB
Inferred from direct assayⁱ
- 16288031
protein K48-linked ubiquitination Source: UniProtKB
protein K63-linked ubiquitination Source: UniProtKB
protein polyubiquitination Source: Reactome
protein ubiquitination
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23418353

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Ubl conjugation pathway
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayⁱ	UPA00143

Protein family/group databases

MoonDBⁱ	Q9Y4L5 Predicted

MoonDBⁱ

Q9Y4L5 Predicted

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: E3 ubiquitin-protein ligase RNF115Curated (EC:2.3.2.271 Publication) Alternative name(s): RING finger protein 115Imported RING-type E3 ubiquitin transferase RNF115Curated Rabring 7By similarity Zinc finger protein 3641 Publication
Gene namesⁱ	Name:RNF115Imported Synonyms:ZNF3641 Publication
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000265491.4
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:18154 RNF115
neXtProtⁱ	NX_Q9Y4L5

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	26	K → R: Loss of autoubiquitination; when associated with R-32. 1 Publication	1
Mutagenesisⁱ	32	K → R: Loss of autoubiquitination; when associated with R-26. 1 Publication	1
Mutagenesisⁱ	228	C → A: Loss of autoubiquitination. 1 Publication	1
Mutagenesisⁱ	231	C → A: Loss of autoubiquitination. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	27246
Open Targets More...OpenTargetsⁱ	ENSG00000265491
PharmGKBⁱ	PA162401519

Polymorphism and mutation databases

BioMutaⁱ	RNF115
DMDMⁱ	56405389

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000056314	2 – 304	E3 ubiquitin-protein ligase RNF115Add BLAST		303

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	2	N-acetylalanineCombined sources		1

Post-translational modificationⁱ

Keywords - PTMⁱ

Acetylation, Ubl conjugation

Proteomic databases

EPDⁱ	Q9Y4L5
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9Y4L5
PaxDbⁱ	Q9Y4L5
PeptideAtlas More...PeptideAtlasⁱ	Q9Y4L5
PRIDEⁱ	Q9Y4L5
ProteomicsDBⁱ	86228

PTM databases

iPTMnetⁱ	Q9Y4L5
PhosphoSitePlusⁱ	Q9Y4L5

Expressionⁱ

Tissue specificityⁱ

Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000265491 Expressed in 237 organ(s), highest expression level in gluteal muscle
CleanExⁱ	HS_RNF115
Genevisibleⁱ	Q9Y4L5 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA019130

HPAⁱ

HPA019130

Interactionⁱ

Subunit structureⁱ

Interacts with RAB7A. Interacts with EGFR and FLT3.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
DAZAP2	Q15038	4	EBI-2129242,EBI-724310
MDM4	O15151	3	EBI-2129242,EBI-398437
UBE2D1	P51668	8	EBI-2129242,EBI-743540
UBE2D2	P62837	5	EBI-2129242,EBI-347677
UBE2D3	P61077	6	EBI-2129242,EBI-348268
UBE2D4	Q9Y2X8	6	EBI-2129242,EBI-745527
UBE2E2	Q96LR5	4	EBI-2129242,EBI-2129763
UBE2E3	Q969T4	3	EBI-2129242,EBI-348496

Protein-protein interaction databases

BioGridⁱ	118094, 52 interactors
IntActⁱ	Q9Y4L5, 10 interactors
Molecular INTeraction database More...MINTⁱ	Q9Y4L5
STRINGⁱ	9606.ENSP00000358297

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9Y4L5
SMRⁱ	Q9Y4L5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	228 – 269	RING-typePROSITE-ProRule annotationAdd BLAST		42

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG0800 Eukaryota ENOG41121N2 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000062967
HOGENOMⁱ	HOG000116417
HOVERGENⁱ	HBG059832
InParanoidⁱ	Q9Y4L5
KEGG Orthology (KO) More...KOⁱ	K11982
OMAⁱ	SSHPFSW
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0SOO
PhylomeDBⁱ	Q9Y4L5
TreeFamⁱ	TF317985

Family and domain databases

Gene3Dⁱ	3.30.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13639 zf-RING_2, 1 hit
SMARTⁱ	View protein in SMART SM00184 RING, 1 hit
PROSITEⁱ	View protein in PROSITE PS50089 ZF_RING_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9Y4L5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAEASAAGAD SGAAVAAHRF FCHFCKGEVS PKLPEYICPR CESGFIEEVT 
        60         70         80         90        100
DDSSFLGGGG SRIDNTTTTH FAELWGHLDH TMFFQDFRPF LSSSPLDQDN 
       110        120        130        140        150
RANERGHQTH TDFWGARPPR LPLGRRYRSR GSSRPDRSPA IEGILQHIFA 
       160        170        180        190        200
GFFANSAIPG SPHPFSWSGM LHSNPGDYAW GQTGLDAIVT QLLGQLENTG 
       210        220        230        240        250
PPPADKEKIT SLPTVTVTQE QVDMGLECPV CKEDYTVEEE VRQLPCNHFF 
       260        270        280        290        300
HSSCIVPWLE LHDTCPVCRK SLNGEDSTRQ SQSTEASASN RFSNDSQLHD 

RWTF

Length:304

Mass (Da):33,703

Last modified:December 7, 2004 - v2

Checksum:ⁱ22F0C8FCC0F55045

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	299	H → N in CAB45280 (Ref. 7) Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_052105	194	G → R. Corresponds to variant dbSNP:rs11577731Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF542552 mRNA Translation: AAQ09535.1 AF419857 mRNA Translation: AAP97292.1 AK290749 mRNA Translation: BAF83438.1 AL390725, AL160282 Genomic DNA Translation: CAI13717.1 CH471244 Genomic DNA Translation: EAW71437.1 BC054049 mRNA Translation: AAH54049.1 BC064903 mRNA Translation: AAH64903.1 AL079314 mRNA Translation: CAB45280.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS72863.1
NCBI Reference Sequences More...RefSeqⁱ	NP_055270.1, NM_014455.3
UniGeneⁱ	Hs.523550

Genome annotation databases

Ensemblⁱ	ENST00000582693; ENSP00000463650; ENSG00000265491
GeneIDⁱ	27246
KEGGⁱ	hsa:27246
UCSC genome browser More...UCSCⁱ	uc031utf.2 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9Y4L5	Ring finger protein 115		MUSPF		320	UniRef90_Q9Y4L5
Ring finger protein 115		CANLF		319
E3 ubiquitin-protein ligase RNF115		URSMA		316
E3 ubiquitin-protein ligase RNF115		FUKDA		306
Uncharacterized protein		OTOGA		306
+78

Protein	Similar proteins		Species	Score	Length	Source
Q9Y4L5	E3 ubiquitin-protein ligase RNF115		MOUSE		305	UniRef50_Q9Y4L5
Ring finger protein 115		MUSPF		320
Ring finger protein 115		CANLF		319
E3 ubiquitin-protein ligase RNF115		URSMA		316
E3 ubiquitin-protein ligase RNF115		HETGA		306
+171

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF542552 mRNA Translation: AAQ09535.1 AF419857 mRNA Translation: AAP97292.1 AK290749 mRNA Translation: BAF83438.1 AL390725, AL160282 Genomic DNA Translation: CAI13717.1 CH471244 Genomic DNA Translation: EAW71437.1 BC054049 mRNA Translation: AAH54049.1 BC064903 mRNA Translation: AAH64903.1 AL079314 mRNA Translation: CAB45280.1
CCDSⁱ	CCDS72863.1
RefSeqⁱ	NP_055270.1, NM_014455.3
UniGeneⁱ	Hs.523550

3D structure databases

ProteinModelPortalⁱ	Q9Y4L5
SMRⁱ	Q9Y4L5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	118094, 52 interactors
IntActⁱ	Q9Y4L5, 10 interactors
MINTⁱ	Q9Y4L5
STRINGⁱ	9606.ENSP00000358297

Protein family/group databases

MoonDBⁱ	Q9Y4L5 Predicted

MoonDBⁱ

Q9Y4L5 Predicted

PTM databases

iPTMnetⁱ	Q9Y4L5
PhosphoSitePlusⁱ	Q9Y4L5

Polymorphism and mutation databases

BioMutaⁱ	RNF115
DMDMⁱ	56405389

Proteomic databases

EPDⁱ	Q9Y4L5
MaxQBⁱ	Q9Y4L5
PaxDbⁱ	Q9Y4L5
PeptideAtlasⁱ	Q9Y4L5
PRIDEⁱ	Q9Y4L5
ProteomicsDBⁱ	86228

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	27246
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000582693; ENSP00000463650; ENSG00000265491
GeneIDⁱ	27246
KEGGⁱ	hsa:27246
UCSCⁱ	uc031utf.2 human

Organism-specific databases

CTDⁱ	27246
DisGeNETⁱ	27246
EuPathDBⁱ	HostDB:ENSG00000265491.4
GeneCardsⁱ	RNF115
HGNCⁱ	HGNC:18154 RNF115
HPAⁱ	HPA019130
neXtProtⁱ	NX_Q9Y4L5
OpenTargetsⁱ	ENSG00000265491
PharmGKBⁱ	PA162401519
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0800 Eukaryota ENOG41121N2 LUCA
GeneTreeⁱ	ENSGT00530000062967
HOGENOMⁱ	HOG000116417
HOVERGENⁱ	HBG059832
InParanoidⁱ	Q9Y4L5
KOⁱ	K11982
OMAⁱ	SSHPFSW
OrthoDBⁱ	EOG091G0SOO
PhylomeDBⁱ	Q9Y4L5
TreeFamⁱ	TF317985

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
Reactomeⁱ	R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSⁱ	RNF115 human
GeneWikiⁱ	ZNF364
GenomeRNAiⁱ	27246
Protein Ontology More...PROⁱ	PR:Q9Y4L5

Gene expression databases

Bgeeⁱ	ENSG00000265491 Expressed in 237 organ(s), highest expression level in gluteal muscle
CleanExⁱ	HS_RNF115
Genevisibleⁱ	Q9Y4L5 HS

Family and domain databases

Gene3Dⁱ	3.30.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD
Pfamⁱ	View protein in Pfam PF13639 zf-RING_2, 1 hit
SMARTⁱ	View protein in SMART SM00184 RING, 1 hit
PROSITEⁱ	View protein in PROSITE PS50089 ZF_RING_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RN115_HUMAN
Accessionⁱ	Q9Y4L5Primary (citable) accession number: Q9Y4L5 Secondary accession number(s): A8K3Y4, Q5T2V9, Q7Z2J2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
	Last sequence update:	December 7, 2004
	Last modified:	November 7, 2018
	This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
PATHWAY comments
Index of metabolic and biosynthesis pathways

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9Y4L5 (RN115_HUMAN)

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E3 ubiquitin-protein ligase RNF115

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytosol

Cytosol

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ