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UniProtKB - Q9Y4L5 (RN115_HUMAN)
Protein
E3 ubiquitin-protein ligase RNF115
Gene
RNF115
Organism
Homo sapiens (Human)
Status
Functioni
E3 ubiquitin-protein ligase that mediates E2-dependent, 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of substrates and may play a role in diverse biological processes. Through their polyubiquitination, may play a role in the endosomal trafficking and degradation of membrane receptors including EGFR, FLT3, MET and CXCR4.
1 Publication2 PublicationsCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 228 – 269 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: UniProtKB
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- negative regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
- protein autoubiquitination Source: UniProtKB
- protein K48-linked ubiquitination Source: UniProtKB
- protein K63-linked ubiquitination Source: UniProtKB
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9Y4L5 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q9Y4L5 |
UniPathwayi | UPA00143 |
Protein family/group databases
MoonDBi | Q9Y4L5, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RNF115Curated (EC:2.3.2.271 Publication)Alternative name(s): RING finger protein 115Imported RING-type E3 ubiquitin transferase RNF115Curated Rab7-interacting RING finger proteinBy similarity Short name: Rabring 7By similarity Zinc finger protein 3641 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18154, RNF115 |
MIMi | 619535, gene |
neXtProti | NX_Q9Y4L5 |
VEuPathDBi | HostDB:ENSG00000265491 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol By similarity
Note: The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes.By similarity
Cytosol
- cytosol Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 26 | K → R: Loss of autoubiquitination; when associated with R-32. 1 Publication | 1 | |
Mutagenesisi | 32 | K → R: Loss of autoubiquitination; when associated with R-26. 1 Publication | 1 | |
Mutagenesisi | 228 | C → A: Loss of autoubiquitination. 1 Publication | 1 | |
Mutagenesisi | 231 | C → A: Loss of autoubiquitination. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 27246 |
OpenTargetsi | ENSG00000265491 |
PharmGKBi | PA162401519 |
Miscellaneous databases
Pharosi | Q9Y4L5, Tbio |
Genetic variation databases
BioMutai | RNF115 |
DMDMi | 56405389 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000056314 | 2 – 304 | E3 ubiquitin-protein ligase RNF115Add BLAST | 303 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Post-translational modificationi
RING-type zinc finger-dependent and E2-dependent autoubiquitination.2 Publications
Keywords - PTMi
Acetylation, Ubl conjugationProteomic databases
EPDi | Q9Y4L5 |
jPOSTi | Q9Y4L5 |
MassIVEi | Q9Y4L5 |
MaxQBi | Q9Y4L5 |
PaxDbi | Q9Y4L5 |
PeptideAtlasi | Q9Y4L5 |
PRIDEi | Q9Y4L5 |
ProteomicsDBi | 86228 |
PTM databases
iPTMneti | Q9Y4L5 |
PhosphoSitePlusi | Q9Y4L5 |
Expressioni
Tissue specificityi
Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells.1 Publication
Gene expression databases
Bgeei | ENSG00000265491, Expressed in gluteal muscle and 250 other tissues |
Genevisiblei | Q9Y4L5, HS |
Organism-specific databases
HPAi | ENSG00000265491, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with RAB7A.
Interacts with EGFR and FLT3.
2 PublicationsBinary interactionsi
Q9Y4L5
Protein-protein interaction databases
BioGRIDi | 118094, 122 interactors |
IntActi | Q9Y4L5, 28 interactors |
MINTi | Q9Y4L5 |
STRINGi | 9606.ENSP00000463650 |
Miscellaneous databases
RNActi | Q9Y4L5, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 95 – 138 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 272 – 304 | DisorderedSequence analysisAdd BLAST | 33 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 228 – 269 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000157203 |
HOGENOMi | CLU_034892_0_2_1 |
InParanoidi | Q9Y4L5 |
OMAi | RMDNSTS |
OrthoDBi | 1249953at2759 |
PhylomeDBi | Q9Y4L5 |
TreeFami | TF317985 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9Y4L5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEASAAGAD SGAAVAAHRF FCHFCKGEVS PKLPEYICPR CESGFIEEVT
60 70 80 90 100
DDSSFLGGGG SRIDNTTTTH FAELWGHLDH TMFFQDFRPF LSSSPLDQDN
110 120 130 140 150
RANERGHQTH TDFWGARPPR LPLGRRYRSR GSSRPDRSPA IEGILQHIFA
160 170 180 190 200
GFFANSAIPG SPHPFSWSGM LHSNPGDYAW GQTGLDAIVT QLLGQLENTG
210 220 230 240 250
PPPADKEKIT SLPTVTVTQE QVDMGLECPV CKEDYTVEEE VRQLPCNHFF
260 270 280 290 300
HSSCIVPWLE LHDTCPVCRK SLNGEDSTRQ SQSTEASASN RFSNDSQLHD
RWTF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 299 | H → N in CAB45280 (Ref. 7) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052105 | 194 | G → R. Corresponds to variant dbSNP:rs11577731Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF542552 mRNA Translation: AAQ09535.1 AF419857 mRNA Translation: AAP97292.1 AK290749 mRNA Translation: BAF83438.1 AL390725, AL160282 Genomic DNA Translation: CAI13717.1 CH471244 Genomic DNA Translation: EAW71437.1 BC054049 mRNA Translation: AAH54049.1 BC064903 mRNA Translation: AAH64903.1 AL079314 mRNA Translation: CAB45280.1 |
CCDSi | CCDS72863.1 |
RefSeqi | NP_055270.1, NM_014455.3 |
Genome annotation databases
Ensembli | ENST00000582693.5; ENSP00000463650.1; ENSG00000265491.5 |
GeneIDi | 27246 |
KEGGi | hsa:27246 |
MANE-Selecti | ENST00000582693.5; ENSP00000463650.1; NM_014455.4; NP_055270.1 |
UCSCi | uc031utf.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF542552 mRNA Translation: AAQ09535.1 AF419857 mRNA Translation: AAP97292.1 AK290749 mRNA Translation: BAF83438.1 AL390725, AL160282 Genomic DNA Translation: CAI13717.1 CH471244 Genomic DNA Translation: EAW71437.1 BC054049 mRNA Translation: AAH54049.1 BC064903 mRNA Translation: AAH64903.1 AL079314 mRNA Translation: CAB45280.1 |
CCDSi | CCDS72863.1 |
RefSeqi | NP_055270.1, NM_014455.3 |
3D structure databases
AlphaFoldDBi | Q9Y4L5 |
SMRi | Q9Y4L5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 118094, 122 interactors |
IntActi | Q9Y4L5, 28 interactors |
MINTi | Q9Y4L5 |
STRINGi | 9606.ENSP00000463650 |
Protein family/group databases
MoonDBi | Q9Y4L5, Predicted |
PTM databases
iPTMneti | Q9Y4L5 |
PhosphoSitePlusi | Q9Y4L5 |
Genetic variation databases
BioMutai | RNF115 |
DMDMi | 56405389 |
Proteomic databases
EPDi | Q9Y4L5 |
jPOSTi | Q9Y4L5 |
MassIVEi | Q9Y4L5 |
MaxQBi | Q9Y4L5 |
PaxDbi | Q9Y4L5 |
PeptideAtlasi | Q9Y4L5 |
PRIDEi | Q9Y4L5 |
ProteomicsDBi | 86228 |
Protocols and materials databases
Antibodypediai | 74554, 102 antibodies from 24 providers |
DNASUi | 27246 |
Genome annotation databases
Ensembli | ENST00000582693.5; ENSP00000463650.1; ENSG00000265491.5 |
GeneIDi | 27246 |
KEGGi | hsa:27246 |
MANE-Selecti | ENST00000582693.5; ENSP00000463650.1; NM_014455.4; NP_055270.1 |
UCSCi | uc031utf.2, human |
Organism-specific databases
CTDi | 27246 |
DisGeNETi | 27246 |
GeneCardsi | RNF115 |
HGNCi | HGNC:18154, RNF115 |
HPAi | ENSG00000265491, Low tissue specificity |
MIMi | 619535, gene |
neXtProti | NX_Q9Y4L5 |
OpenTargetsi | ENSG00000265491 |
PharmGKBi | PA162401519 |
VEuPathDBi | HostDB:ENSG00000265491 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000157203 |
HOGENOMi | CLU_034892_0_2_1 |
InParanoidi | Q9Y4L5 |
OMAi | RMDNSTS |
OrthoDBi | 1249953at2759 |
PhylomeDBi | Q9Y4L5 |
TreeFami | TF317985 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q9Y4L5 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q9Y4L5 |
Miscellaneous databases
BioGRID-ORCSi | 27246, 20 hits in 1111 CRISPR screens |
ChiTaRSi | RNF115, human |
GeneWikii | ZNF364 |
GenomeRNAii | 27246 |
Pharosi | Q9Y4L5, Tbio |
PROi | PR:Q9Y4L5 |
RNActi | Q9Y4L5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000265491, Expressed in gluteal muscle and 250 other tissues |
Genevisiblei | Q9Y4L5, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RN115_HUMAN | |
Accessioni | Q9Y4L5Primary (citable) accession number: Q9Y4L5 Secondary accession number(s): A8K3Y4, Q5T2V9, Q7Z2J2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 12, 2003 |
Last sequence update: | December 7, 2004 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways