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Protein

Disheveled-associated activator of morphogenesis 1

Gene

DAAM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia (PubMed:16630611, PubMed:17482208). Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity).By similarity2 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • Rho GTPase binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-HSA-4086400 PCP/CE pathway
R-HSA-5663220 RHO GTPases Activate Formins
SignaLinkiQ9Y4D1
SIGNORiQ9Y4D1

Names & Taxonomyi

Protein namesi
Recommended name:
Disheveled-associated activator of morphogenesis 1
Gene namesi
Name:DAAM1
Synonyms:KIAA0666
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000100592.15
HGNCiHGNC:18142 DAAM1
MIMi606626 gene
neXtProtiNX_Q9Y4D1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi698I → A: Abolishes actin-binding. 1 Publication1

Organism-specific databases

DisGeNETi23002
OpenTargetsiENSG00000100592
PharmGKBiPA27129

Polymorphism and mutation databases

BioMutaiDAAM1
DMDMi34098767

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949071 – 1078Disheveled-associated activator of morphogenesis 1Add BLAST1078

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineCombined sources1
Modified residuei1027PhosphoserineCombined sources1
Modified residuei1030PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y4D1
PaxDbiQ9Y4D1
PeptideAtlasiQ9Y4D1
PRIDEiQ9Y4D1
ProteomicsDBi86164
86165 [Q9Y4D1-2]
86166 [Q9Y4D1-3]

PTM databases

iPTMnetiQ9Y4D1
PhosphoSitePlusiQ9Y4D1
SwissPalmiQ9Y4D1

Expressioni

Tissue specificityi

Expressed in all tissues examined.

Gene expression databases

BgeeiENSG00000100592 Expressed in 232 organ(s), highest expression level in neocortex
CleanExiHS_DAAM1
ExpressionAtlasiQ9Y4D1 baseline and differential
GenevisibleiQ9Y4D1 HS

Organism-specific databases

HPAiHPA026605
HPA064789

Interactioni

Subunit structurei

Homodimer. Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA. Interacts with INTU; INTU mediates the indirect interaction between DAAM1 and NPHP4.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116648, 32 interactors
DIPiDIP-29641N
IntActiQ9Y4D1, 24 interactors
MINTiQ9Y4D1
STRINGi9606.ENSP00000247170

Structurei

Secondary structure

11078
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y4D1
SMRiQ9Y4D1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y4D1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 420GBD/FH3PROSITE-ProRule annotationAdd BLAST376
Domaini528 – 599FH1Add BLAST72
Domaini600 – 1009FH2PROSITE-ProRule annotationAdd BLAST410
Domaini1027 – 1058DADPROSITE-ProRule annotationAdd BLAST32

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni693 – 702Actin-binding10

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili437 – 526Sequence analysisAdd BLAST90

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi528 – 593Pro-richAdd BLAST66

Domaini

The C-terminal DAD domain may participate in intramolecular interactions with the N-terminus.
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1922 Eukaryota
ENOG410XT5Z LUCA
GeneTreeiENSGT00760000118986
HOGENOMiHOG000237318
HOVERGENiHBG101333
InParanoidiQ9Y4D1
KOiK04512
OMAiKMPVFRG
OrthoDBiEOG091G02QJ
PhylomeDBiQ9Y4D1
TreeFamiTF314602

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR027650 DAAM1
IPR014767 DAD_dom
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
PANTHERiPTHR23213:SF181 PTHR23213:SF181, 1 hit
PfamiView protein in Pfam
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 1 hit
PF02181 FH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51231 DAD, 1 hit
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y4D1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPRKRGGRG ISFIFCCFRN NDHPEITYRL RNDSNFALQT MEPALPMPPV
60 70 80 90 100
EELDVMFSEL VDELDLTDKH REAMFALPAE KKWQIYCSKK KDQEENKGAT
110 120 130 140 150
SWPEFYIDQL NSMAARKSLL ALEKEEEEER SKTIESLKTA LRTKPMRFVT
160 170 180 190 200
RFIDLDGLSC ILNFLKTMDY ETSESRIHTS LIGCIKALMN NSQGRAHVLA
210 220 230 240 250
HSESINVIAQ SLSTENIKTK VAVLEILGAV CLVPGGHKKV LQAMLHYQKY
260 270 280 290 300
ASERTRFQTL INDLDKSTGR YRDEVSLKTA IMSFINAVLS QGAGVESLDF
310 320 330 340 350
RLHLRYEFLM LGIQPVIDKL REHENSTLDR HLDFFEMLRN EDELEFAKRF
360 370 380 390 400
ELVHIDTKSA TQMFELTRKR LTHSEAYPHF MSILHHCLQM PYKRSGNTVQ
410 420 430 440 450
YWLLLDRIIQ QIVIQNDKGQ DPDSTPLENF NIKNVVRMLV NENEVKQWKE
460 470 480 490 500
QAEKMRKEHN ELQQKLEKKE RECDAKTQEK EEMMQTLNKM KEKLEKETTE
510 520 530 540 550
HKQVKQQVAD LTAQLHELSR RAVCASIPGG PSPGAPGGPF PSSVPGSLLP
560 570 580 590 600
PPPPPPLPGG MLPPPPPPLP PGGPPPPPGP PPLGAIMPPP GAPMGLALKK
610 620 630 640 650
KSIPQPTNAL KSFNWSKLPE NKLEGTVWTE IDDTKVFKIL DLEDLERTFS
660 670 680 690 700
AYQRQQDFFV NSNSKQKEAD AIDDTLSSKL KVKELSVIDG RRAQNCNILL
710 720 730 740 750
SRLKLSNDEI KRAILTMDEQ EDLPKDMLEQ LLKFVPEKSD IDLLEEHKHE
760 770 780 790 800
LDRMAKADRF LFEMSRINHY QQRLQSLYFK KKFAERVAEV KPKVEAIRSG
810 820 830 840 850
SEEVFRSGAL KQLLEVVLAF GNYMNKGQRG NAYGFKISSL NKIADTKSSI
860 870 880 890 900
DKNITLLHYL ITIVENKYPS VLNLNEELRD IPQAAKVNMT ELDKEISTLR
910 920 930 940 950
SGLKAVETEL EYQKSQPPQP GDKFVSVVSQ FITVASFSFS DVEDLLAEAK
960 970 980 990 1000
DLFTKAVKHF GEEAGKIQPD EFFGIFDQFL QAVSEAKQEN ENMRKKKEEE
1010 1020 1030 1040 1050
ERRARMEAQL KEQRERERKM RKAKENSEES GEFDDLVSAL RSGEVFDKDL
1060 1070
SKLKRNRKRI TNQMTDSSRE RPITKLNF
Length:1,078
Mass (Da):123,473
Last modified:August 15, 2003 - v2
Checksum:iE82D9892390254B6
GO
Isoform 2 (identifier: Q9Y4D1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     656-665: Missing.

Show »
Length:1,068
Mass (Da):122,306
Checksum:iC58B1A7445080313
GO
Isoform 3 (identifier: Q9Y4D1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     788-818: Missing.
     888-916: NMTELDKEISTLRSGLKAVETELEYQKSQ → KSWNIRSLSPHSPEISLCLLSASSSQ
     917-1078: Missing.

Show »
Length:882
Mass (Da):100,745
Checksum:i1BB2D1876B212852
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V275G3V275_HUMAN
Disheveled-associated activator of ...
DAAM1
96Annotation score:

Sequence cautioni

The sequence BAA31641 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC04230 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti940S → F in AAH24781 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008000656 – 665Missing in isoform 2. 1 Publication10
Alternative sequenceiVSP_008001788 – 818Missing in isoform 3. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_008002888 – 916NMTEL…YQKSQ → KSWNIRSLSPHSPEISLCLL SASSSQ in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_008003917 – 1078Missing in isoform 3. 1 PublicationAdd BLAST162

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014566 mRNA Translation: BAA31641.1 Different initiation.
AL133502 Genomic DNA No translation available.
BC024781 mRNA Translation: AAH24781.1
BC038428 mRNA Translation: AAH38428.1
BC064999 mRNA Translation: AAH64999.1
BX247986 mRNA Translation: CAD62320.1
AK093813 mRNA Translation: BAC04230.1 Different initiation.
CCDSiCCDS58323.1 [Q9Y4D1-2]
CCDS9737.1 [Q9Y4D1-1]
RefSeqiNP_001257449.1, NM_001270520.1 [Q9Y4D1-2]
NP_055807.1, NM_014992.2 [Q9Y4D1-1]
XP_005267487.1, XM_005267430.1 [Q9Y4D1-1]
XP_005267488.1, XM_005267431.1 [Q9Y4D1-1]
UniGeneiHs.19156
Hs.654934

Genome annotation databases

EnsembliENST00000360909; ENSP00000354162; ENSG00000100592 [Q9Y4D1-2]
ENST00000395125; ENSP00000378557; ENSG00000100592 [Q9Y4D1-1]
GeneIDi23002
KEGGihsa:23002
UCSCiuc001xea.3 human [Q9Y4D1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014566 mRNA Translation: BAA31641.1 Different initiation.
AL133502 Genomic DNA No translation available.
BC024781 mRNA Translation: AAH24781.1
BC038428 mRNA Translation: AAH38428.1
BC064999 mRNA Translation: AAH64999.1
BX247986 mRNA Translation: CAD62320.1
AK093813 mRNA Translation: BAC04230.1 Different initiation.
CCDSiCCDS58323.1 [Q9Y4D1-2]
CCDS9737.1 [Q9Y4D1-1]
RefSeqiNP_001257449.1, NM_001270520.1 [Q9Y4D1-2]
NP_055807.1, NM_014992.2 [Q9Y4D1-1]
XP_005267487.1, XM_005267430.1 [Q9Y4D1-1]
XP_005267488.1, XM_005267431.1 [Q9Y4D1-1]
UniGeneiHs.19156
Hs.654934

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J1DX-ray2.55G596-1078[»]
2Z6EX-ray2.80A/B/C/D594-1012[»]
ProteinModelPortaliQ9Y4D1
SMRiQ9Y4D1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116648, 32 interactors
DIPiDIP-29641N
IntActiQ9Y4D1, 24 interactors
MINTiQ9Y4D1
STRINGi9606.ENSP00000247170

PTM databases

iPTMnetiQ9Y4D1
PhosphoSitePlusiQ9Y4D1
SwissPalmiQ9Y4D1

Polymorphism and mutation databases

BioMutaiDAAM1
DMDMi34098767

Proteomic databases

EPDiQ9Y4D1
PaxDbiQ9Y4D1
PeptideAtlasiQ9Y4D1
PRIDEiQ9Y4D1
ProteomicsDBi86164
86165 [Q9Y4D1-2]
86166 [Q9Y4D1-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360909; ENSP00000354162; ENSG00000100592 [Q9Y4D1-2]
ENST00000395125; ENSP00000378557; ENSG00000100592 [Q9Y4D1-1]
GeneIDi23002
KEGGihsa:23002
UCSCiuc001xea.3 human [Q9Y4D1-1]

Organism-specific databases

CTDi23002
DisGeNETi23002
EuPathDBiHostDB:ENSG00000100592.15
GeneCardsiDAAM1
H-InvDBiHIX0011701
HGNCiHGNC:18142 DAAM1
HPAiHPA026605
HPA064789
MIMi606626 gene
neXtProtiNX_Q9Y4D1
OpenTargetsiENSG00000100592
PharmGKBiPA27129
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1922 Eukaryota
ENOG410XT5Z LUCA
GeneTreeiENSGT00760000118986
HOGENOMiHOG000237318
HOVERGENiHBG101333
InParanoidiQ9Y4D1
KOiK04512
OMAiKMPVFRG
OrthoDBiEOG091G02QJ
PhylomeDBiQ9Y4D1
TreeFamiTF314602

Enzyme and pathway databases

ReactomeiR-HSA-4086400 PCP/CE pathway
R-HSA-5663220 RHO GTPases Activate Formins
SignaLinkiQ9Y4D1
SIGNORiQ9Y4D1

Miscellaneous databases

ChiTaRSiDAAM1 human
EvolutionaryTraceiQ9Y4D1
GeneWikiiDAAM1
GenomeRNAii23002
PROiPR:Q9Y4D1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100592 Expressed in 232 organ(s), highest expression level in neocortex
CleanExiHS_DAAM1
ExpressionAtlasiQ9Y4D1 baseline and differential
GenevisibleiQ9Y4D1 HS

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR027650 DAAM1
IPR014767 DAD_dom
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
PANTHERiPTHR23213:SF181 PTHR23213:SF181, 1 hit
PfamiView protein in Pfam
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 1 hit
PF02181 FH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51231 DAD, 1 hit
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDAAM1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y4D1
Secondary accession number(s): Q86U34, Q8N1Z8, Q8TB39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: August 15, 2003
Last modified: November 7, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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