UniProtKB - Q9Y478 (AAKB1_HUMAN)
5'-AMP-activated protein kinase subunit beta-1
PRKAB1
Functioni
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
GO - Molecular functioni
- protein kinase activity Source: UniProtKB
- protein kinase binding Source: GO_Central
GO - Biological processi
- cellular response to nutrient levels Source: ComplexPortal
- fatty acid biosynthetic process Source: UniProtKB-KW
- nail development Source: Ensembl
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of gene expression Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- regulation of catalytic activity Source: InterPro
- signal transduction Source: GO_Central
Keywordsi
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.7.11.31, 2681 |
PathwayCommonsi | Q9Y478 |
Reactomei | R-HSA-1445148, Translocation of SLC2A4 (GLUT4) to the plasma membrane R-HSA-1632852, Macroautophagy R-HSA-2151209, Activation of PPARGC1A (PGC-1alpha) by phosphorylation R-HSA-380972, Energy dependent regulation of mTOR by LKB1-AMPK R-HSA-5628897, TP53 Regulates Metabolic Genes R-HSA-6804756, Regulation of TP53 Activity through Phosphorylation R-HSA-9613354, Lipophagy R-HSA-9619483, Activation of AMPK downstream of NMDARs |
SignaLinki | Q9Y478 |
SIGNORi | Q9Y478 |
Protein family/group databases
CAZyi | CBM48, Carbohydrate-Binding Module Family 48 |
Names & Taxonomyi
Protein namesi | Recommended name: 5'-AMP-activated protein kinase subunit beta-1Short name: AMPK subunit beta-1 Short name: AMPKb |
Gene namesi | Name:PRKAB1 Synonyms:AMPK |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9378, PRKAB1 |
MIMi | 602740, gene |
neXtProti | NX_Q9Y478 |
VEuPathDBi | HostDB:ENSG00000111725 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: ComplexPortal
Other locations
- cytoplasm Source: GO_Central
- nucleotide-activated protein kinase complex Source: ComplexPortal
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | G → A: Abolishes myristoylation and AMP-enhanced phosphorylation of PRKAA1 or PRKAA2. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5564 |
OpenTargetsi | ENSG00000111725 |
PharmGKBi | PA33746 |
Miscellaneous databases
Pharosi | Q9Y478, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3847 |
DrugBanki | DB00945, Acetylsalicylic acid DB00131, Adenosine phosphate DB12010, Fostamatinib DB00331, Metformin DB00273, Topiramate |
DrugCentrali | Q9Y478 |
GuidetoPHARMACOLOGYi | 1543 |
Genetic variation databases
BioMutai | PRKAB1 |
DMDMi | 14194425 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000204363 | 2 – 270 | 5'-AMP-activated protein kinase subunit beta-1Add BLAST | 269 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Modified residuei | 4 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 5 | PhosphoserineCombined sources | 1 | |
Modified residuei | 6 | PhosphoserineCombined sources | 1 | |
Modified residuei | 19 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 24 | Phosphoserine; by autocatalysisBy similarity | 1 | |
Modified residuei | 25 | Phosphoserine; by autocatalysisBy similarity | 1 | |
Modified residuei | 40 | PhosphoserineCombined sources | 1 | |
Modified residuei | 96 | PhosphoserineCombined sources | 1 | |
Modified residuei | 101 | PhosphoserineBy similarity | 1 | |
Modified residuei | 108 | PhosphoserineCombined sources | 1 | |
Modified residuei | 148 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 182 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
EPDi | Q9Y478 |
jPOSTi | Q9Y478 |
MassIVEi | Q9Y478 |
MaxQBi | Q9Y478 |
PaxDbi | Q9Y478 |
PeptideAtlasi | Q9Y478 |
PRIDEi | Q9Y478 |
ProteomicsDBi | 86122 |
PTM databases
iPTMneti | Q9Y478 |
PhosphoSitePlusi | Q9Y478 |
SwissPalmi | Q9Y478 |
Expressioni
Gene expression databases
Bgeei | ENSG00000111725, Expressed in adult mammalian kidney and 211 other tissues |
ExpressionAtlasi | Q9Y478, baseline and differential |
Genevisiblei | Q9Y478, HS |
Organism-specific databases
HPAi | ENSG00000111725, Low tissue specificity |
Interactioni
Subunit structurei
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3).
Interacts with FNIP1 and FNIP2.
3 PublicationsBinary interactionsi
Q9Y478
GO - Molecular functioni
- protein kinase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 111551, 91 interactors |
ComplexPortali | CPX-5633, AMPK complex, alpha1-beta1-gamma1 variant CPX-5786, AMPK complex, alpha1-beta1-gamma2 variant CPX-5787, AMPK complex, alpha2-beta1-gamma1 variant CPX-5842, AMPK complex, alpha1-beta1-gamma3 variant CPX-5843, AMPK complex, alpha2-beta1-gamma3 variant CPX-5844, AMPK complex, alpha2-beta1-gamma2 variant |
CORUMi | Q9Y478 |
DIPi | DIP-39736N |
IntActi | Q9Y478, 35 interactors |
MINTi | Q9Y478 |
STRINGi | 9606.ENSP00000229328 |
Chemistry databases
BindingDBi | Q9Y478 |
Miscellaneous databases
RNActi | Q9Y478, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9Y478 |
SMRi | Q9Y478 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 43 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 68 – 163 | Glycogen-binding domainBy similarityAdd BLAST | 96 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 10 – 43 | Basic and acidic residuesSequence analysisAdd BLAST | 34 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1616, Eukaryota |
GeneTreei | ENSGT00940000155307 |
HOGENOMi | CLU_070949_2_0_1 |
OMAi | NDRAPTQ |
OrthoDBi | 956412at2759 |
PhylomeDBi | Q9Y478 |
TreeFami | TF313827 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
IDEALi | IID00686 |
InterProi | View protein in InterPro IPR032640, AMPK1_CBM IPR039160, AMPKB IPR006828, ASC_dom IPR037256, ASC_dom_sf IPR013783, Ig-like_fold IPR014756, Ig_E-set |
PANTHERi | PTHR10343:SF89, PTHR10343:SF89, 1 hit |
Pfami | View protein in Pfam PF16561, AMPK1_CBM, 1 hit PF04739, AMPKBI, 1 hit |
SMARTi | View protein in SMART SM01010, AMPKBI, 1 hit |
SUPFAMi | SSF160219, SSF160219, 1 hit SSF81296, SSF81296, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGNTSSERAA LERHGGHKTP RRDSSGGTKD GDRPKILMDS PEDADLFHSE
60 70 80 90 100
EIKAPEKEEF LAWQHDLEVN DKAPAQARPT VFRWTGGGKE VYLSGSFNNW
110 120 130 140 150
SKLPLTRSHN NFVAILDLPE GEHQYKFFVD GQWTHDPSEP IVTSQLGTVN
160 170 180 190 200
NIIQVKKTDF EVFDALMVDS QKCSDVSELS SSPPGPYHQE PYVCKPEERF
210 220 230 240 250
RAPPILPPHL LQVILNKDTG ISCDPALLPE PNHVMLNHLY ALSIKDGVMV
260 270
LSATHRYKKK YVTTLLYKPI
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5H2X8 | F5H2X8_HUMAN | 5'-AMP-activated protein kinase sub... | PRKAB1 | 104 | Annotation score: | ||
F5H610 | F5H610_HUMAN | 5'-AMP-activated protein kinase sub... | PRKAB1 | 62 | Annotation score: | ||
F5H4Y8 | F5H4Y8_HUMAN | 5'-AMP-activated protein kinase sub... | PRKAB1 | 58 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 10 | A → G in CAA73146 (PubMed:9224708).Curated | 1 | |
Sequence conflicti | 10 | A → G in AAC98897 (Ref. 4) Curated | 1 | |
Sequence conflicti | 15 | G → A in CAA12024 (Ref. 1) Curated | 1 | |
Sequence conflicti | 20 | P → A in CAA73146 (PubMed:9224708).Curated | 1 | |
Sequence conflicti | 20 | P → A in AAC98897 (Ref. 4) Curated | 1 | |
Sequence conflicti | 22 | R → K in AAD09237 (Ref. 3) Curated | 1 | |
Sequence conflicti | 22 | R → K in AAD00625 (Ref. 3) Curated | 1 | |
Sequence conflicti | 56 | E → Y in AAD09237 (Ref. 3) Curated | 1 | |
Sequence conflicti | 56 | E → Y in AAD00625 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ224515 mRNA Translation: CAA12024.1 Y12556 mRNA Translation: CAA73146.1 U83994 mRNA Translation: AAD09237.1 U87276 , U87271, U87272, U87273, U87274, U87275 Genomic DNA Translation: AAD00625.1 AF022116 mRNA Translation: AAC98897.1 Frameshift. AC002563 Genomic DNA Translation: AAB71326.1 Sequence problems. BC001007 mRNA Translation: AAH01007.1 BC001056 mRNA Translation: AAH01056.1 BC001823 mRNA Translation: AAH01823.1 BC017671 mRNA Translation: AAH17671.1 |
CCDSi | CCDS9191.1 |
PIRi | T09514 |
RefSeqi | NP_006244.2, NM_006253.4 XP_005253966.1, XM_005253909.1 |
Genome annotation databases
Ensembli | ENST00000229328.10; ENSP00000229328.5; ENSG00000111725.11 ENST00000541640.5; ENSP00000441369.1; ENSG00000111725.11 |
GeneIDi | 5564 |
KEGGi | hsa:5564 |
MANE-Selecti | ENST00000229328.10; ENSP00000229328.5; NM_006253.5; NP_006244.2 |
UCSCi | uc001txg.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ224515 mRNA Translation: CAA12024.1 Y12556 mRNA Translation: CAA73146.1 U83994 mRNA Translation: AAD09237.1 U87276 , U87271, U87272, U87273, U87274, U87275 Genomic DNA Translation: AAD00625.1 AF022116 mRNA Translation: AAC98897.1 Frameshift. AC002563 Genomic DNA Translation: AAB71326.1 Sequence problems. BC001007 mRNA Translation: AAH01007.1 BC001056 mRNA Translation: AAH01056.1 BC001823 mRNA Translation: AAH01823.1 BC017671 mRNA Translation: AAH17671.1 |
CCDSi | CCDS9191.1 |
PIRi | T09514 |
RefSeqi | NP_006244.2, NM_006253.4 XP_005253966.1, XM_005253909.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4CFE | X-ray | 3.02 | B/D | 1-270 | [»] | |
4CFF | X-ray | 3.92 | B/D | 1-270 | [»] | |
4ZHX | X-ray | 2.99 | B/D | 1-270 | [»] | |
5EZV | X-ray | 2.99 | B/D | 1-270 | [»] | |
5ISO | X-ray | 2.63 | B/D | 1-270 | [»] | |
6B1U | X-ray | 2.77 | B/D | 1-270 | [»] | |
6C9F | X-ray | 2.92 | B | 68-270 | [»] | |
6C9G | X-ray | 2.70 | B | 67-270 | [»] | |
6C9H | X-ray | 2.65 | B | 68-270 | [»] | |
6C9J | X-ray | 3.05 | B | 68-270 | [»] | |
AlphaFoldDBi | Q9Y478 | |||||
SMRi | Q9Y478 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111551, 91 interactors |
ComplexPortali | CPX-5633, AMPK complex, alpha1-beta1-gamma1 variant CPX-5786, AMPK complex, alpha1-beta1-gamma2 variant CPX-5787, AMPK complex, alpha2-beta1-gamma1 variant CPX-5842, AMPK complex, alpha1-beta1-gamma3 variant CPX-5843, AMPK complex, alpha2-beta1-gamma3 variant CPX-5844, AMPK complex, alpha2-beta1-gamma2 variant |
CORUMi | Q9Y478 |
DIPi | DIP-39736N |
IntActi | Q9Y478, 35 interactors |
MINTi | Q9Y478 |
STRINGi | 9606.ENSP00000229328 |
Chemistry databases
BindingDBi | Q9Y478 |
ChEMBLi | CHEMBL3847 |
DrugBanki | DB00945, Acetylsalicylic acid DB00131, Adenosine phosphate DB12010, Fostamatinib DB00331, Metformin DB00273, Topiramate |
DrugCentrali | Q9Y478 |
GuidetoPHARMACOLOGYi | 1543 |
Protein family/group databases
CAZyi | CBM48, Carbohydrate-Binding Module Family 48 |
PTM databases
iPTMneti | Q9Y478 |
PhosphoSitePlusi | Q9Y478 |
SwissPalmi | Q9Y478 |
Genetic variation databases
BioMutai | PRKAB1 |
DMDMi | 14194425 |
Proteomic databases
EPDi | Q9Y478 |
jPOSTi | Q9Y478 |
MassIVEi | Q9Y478 |
MaxQBi | Q9Y478 |
PaxDbi | Q9Y478 |
PeptideAtlasi | Q9Y478 |
PRIDEi | Q9Y478 |
ProteomicsDBi | 86122 |
Protocols and materials databases
Antibodypediai | 1328, 829 antibodies from 42 providers |
DNASUi | 5564 |
Genome annotation databases
Ensembli | ENST00000229328.10; ENSP00000229328.5; ENSG00000111725.11 ENST00000541640.5; ENSP00000441369.1; ENSG00000111725.11 |
GeneIDi | 5564 |
KEGGi | hsa:5564 |
MANE-Selecti | ENST00000229328.10; ENSP00000229328.5; NM_006253.5; NP_006244.2 |
UCSCi | uc001txg.4, human |
Organism-specific databases
CTDi | 5564 |
DisGeNETi | 5564 |
GeneCardsi | PRKAB1 |
HGNCi | HGNC:9378, PRKAB1 |
HPAi | ENSG00000111725, Low tissue specificity |
MIMi | 602740, gene |
neXtProti | NX_Q9Y478 |
OpenTargetsi | ENSG00000111725 |
PharmGKBi | PA33746 |
VEuPathDBi | HostDB:ENSG00000111725 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1616, Eukaryota |
GeneTreei | ENSGT00940000155307 |
HOGENOMi | CLU_070949_2_0_1 |
OMAi | NDRAPTQ |
OrthoDBi | 956412at2759 |
PhylomeDBi | Q9Y478 |
TreeFami | TF313827 |
Enzyme and pathway databases
BRENDAi | 2.7.11.31, 2681 |
PathwayCommonsi | Q9Y478 |
Reactomei | R-HSA-1445148, Translocation of SLC2A4 (GLUT4) to the plasma membrane R-HSA-1632852, Macroautophagy R-HSA-2151209, Activation of PPARGC1A (PGC-1alpha) by phosphorylation R-HSA-380972, Energy dependent regulation of mTOR by LKB1-AMPK R-HSA-5628897, TP53 Regulates Metabolic Genes R-HSA-6804756, Regulation of TP53 Activity through Phosphorylation R-HSA-9613354, Lipophagy R-HSA-9619483, Activation of AMPK downstream of NMDARs |
SignaLinki | Q9Y478 |
SIGNORi | Q9Y478 |
Miscellaneous databases
BioGRID-ORCSi | 5564, 19 hits in 1088 CRISPR screens |
ChiTaRSi | PRKAB1, human |
GeneWikii | PRKAB1 |
GenomeRNAii | 5564 |
Pharosi | Q9Y478, Tchem |
PROi | PR:Q9Y478 |
RNActi | Q9Y478, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000111725, Expressed in adult mammalian kidney and 211 other tissues |
ExpressionAtlasi | Q9Y478, baseline and differential |
Genevisiblei | Q9Y478, HS |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
IDEALi | IID00686 |
InterProi | View protein in InterPro IPR032640, AMPK1_CBM IPR039160, AMPKB IPR006828, ASC_dom IPR037256, ASC_dom_sf IPR013783, Ig-like_fold IPR014756, Ig_E-set |
PANTHERi | PTHR10343:SF89, PTHR10343:SF89, 1 hit |
Pfami | View protein in Pfam PF16561, AMPK1_CBM, 1 hit PF04739, AMPKBI, 1 hit |
SMARTi | View protein in SMART SM01010, AMPKBI, 1 hit |
SUPFAMi | SSF160219, SSF160219, 1 hit SSF81296, SSF81296, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AAKB1_HUMAN | |
Accessioni | Q9Y478Primary (citable) accession number: Q9Y478 Secondary accession number(s): Q9UBV0 Q9Y6V8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 2001 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 204 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families