UniProtKB - Q9Y3Y2 (CHTOP_HUMAN)
Chromatin target of PRMT1 protein
CHTOP
Functioni
Plays an important role in the ligand-dependent activation of estrogen receptor target genes (PubMed:19858291).
May play a role in the silencing of fetal globin genes (PubMed:20688955).
Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (By similarity).
Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).
By similarity3 PublicationsRequired for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF1 RNA binding activity (PubMed:23299939).
1 PublicationGO - Molecular functioni
- methyl-CpG binding Source: UniProtKB
- mRNA binding Source: GO_Central
- RNA binding Source: UniProtKB
GO - Biological processi
- in utero embryonic development Source: Ensembl
- mRNA export from nucleus Source: UniProtKB
- positive regulation of ATPase activity Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- positive regulation of helicase activity Source: UniProtKB
- positive regulation of histone methylation Source: UniProtKB
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA transport, Transcription, Transcription regulation, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9Y3Y2 |
Reactomei | R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination |
SignaLinki | Q9Y3Y2 |
Names & Taxonomyi
Protein namesi | Recommended name: Chromatin target of PRMT1 proteinAlternative name(s): Friend of PRMT1 protein Small arginine- and glycine-rich protein Short name: SRAG |
Gene namesi | Name:CHTOP Synonyms:C1orf77, FOP ORF Names:HT031, PP7704 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24511, CHTOP |
MIMi | 614206, gene |
neXtProti | NX_Q9Y3Y2 |
VEuPathDBi | HostDB:ENSG00000160679 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
- nucleolus 1 Publication
- nucleoplasm 1 Publication
- Nucleus speckle 2 Publications
Note: Mostly associated with facultative heterochromatin (By similarity). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332).By similarity1 Publication
Nucleus
- nuclear speck Source: UniProtKB
- nucleolus Source: UniProtKB-SubCell
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
- transcription export complex Source: UniProtKB
Other locations
- cytoplasmic ribonucleoprotein granule Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 195 | R → A: Loss of 5hmc binding; when associated with A-197; A-199; A-201 and A-203. 1 Publication | 1 | |
Mutagenesisi | 197 | R → A: Loss of 5hmc binding; when associated with A-195; A-199; A-201 and A-203. 1 Publication | 1 | |
Mutagenesisi | 199 | R → A: Loss of 5hmc binding; when associated with A-195; A-197; A-201 and A-203. 1 Publication | 1 | |
Mutagenesisi | 201 | R → A: Loss of 5hmc binding; when associated with A-195; A-197; A-199 and A-203. 1 Publication | 1 | |
Mutagenesisi | 203 | R → A: Loss of 5hmc binding; when associated with A-195; A-197; A-199 and A-201. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 26097 |
OpenTargetsi | ENSG00000160679 |
PharmGKBi | PA142672526 |
Miscellaneous databases
Pharosi | Q9Y3Y2, Tbio |
Genetic variation databases
BioMutai | CHTOP |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000089263 | 2 – 248 | Chromatin target of PRMT1 proteinAdd BLAST | 247 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 33 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 40 | PhosphoserineCombined sources | 1 | |
Modified residuei | 49 | PhosphoserineCombined sources | 1 | |
Modified residuei | 64 | PhosphoserineCombined sources | 1 | |
Cross-linki | 70 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 242 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9Y3Y2 |
jPOSTi | Q9Y3Y2 |
MassIVEi | Q9Y3Y2 |
MaxQBi | Q9Y3Y2 |
PaxDbi | Q9Y3Y2 |
PeptideAtlasi | Q9Y3Y2 |
PRIDEi | Q9Y3Y2 |
ProteomicsDBi | 86084 [Q9Y3Y2-1] 86085 [Q9Y3Y2-3] 86086 [Q9Y3Y2-4] |
PTM databases
iPTMneti | Q9Y3Y2 |
MetOSitei | Q9Y3Y2 |
PhosphoSitePlusi | Q9Y3Y2 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000160679, Expressed in tendon of biceps brachii and 245 other tissues |
ExpressionAtlasi | Q9Y3Y2, baseline and differential |
Genevisiblei | Q9Y3Y2, HS |
Organism-specific databases
HPAi | ENSG00000160679, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with PRMT1 and PRMT5 (PubMed:25284789).
Interacts with the 5FMC complex; the interaction is methylation-dependent.
Interacts with FYTTD1, SET and PRC1 complex members CBX4, RNF2 and PHC2; the interactions are methylation-independent.
Interacts with ZNF148 (By similarity).
Component of the transcription/export (TREX) complex at least composed of ALYREF/THOC4, DDX39B, SARNP/CIP29, CHTOP and the THO subcomplex; TREX seems to have dynamic structure involving ATP-dependent remodeling; in the complex interacts (methylated) with ALYREF/THOC4 and with DDX39B in a methylation-independent manner.
Interacts (methylated) with NXF1; the interaction is mutually exclusive with the NXF1:THOC5 interaction (PubMed:23299939, PubMed:23826332).
Interacts with WDR77 and ERH (PubMed:25284789).
By similarity3 PublicationsBinary interactionsi
Q9Y3Y2
Isoform 2 [Q9Y3Y2-3]
Protein-protein interaction databases
BioGRIDi | 117547, 153 interactors |
CORUMi | Q9Y3Y2 |
IntActi | Q9Y3Y2, 76 interactors |
MINTi | Q9Y3Y2 |
STRINGi | 9606.ENSP00000357679 |
Miscellaneous databases
RNActi | Q9Y3Y2, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 151 – 204 | DisorderedSequence analysisAdd BLAST | 54 | |
Regioni | 153 – 206 | Interaction with PRMT1By similarityAdd BLAST | 54 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 194 – 203 | GAR motif; involved in 5hmC binding1 Publication | 10 |
Phylogenomic databases
eggNOGi | ENOG502QV0X, Eukaryota |
GeneTreei | ENSGT00390000002869 |
HOGENOMi | CLU_087638_0_0_1 |
InParanoidi | Q9Y3Y2 |
OMAi | RFTNMMK |
OrthoDBi | 1598721at2759 |
PhylomeDBi | Q9Y3Y2 |
TreeFami | TF331447 |
Family and domain databases
InterProi | View protein in InterPro IPR025715, FoP_C |
Pfami | View protein in Pfam PF13865, FoP_duplication, 1 hit |
SMARTi | View protein in SMART SM01218, FoP_duplication, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAQSAPKVV LKSTTKMSLN ERFTNMLKNK QPTPVNIRAS MQQQQQLASA
60 70 80 90 100
RNRRLAQQME NRPSVQAALK LKQSLKQRLG KSNIQARLGR PIGALARGAI
110 120 130 140 150
GGRGLPIIQR GLPRGGLRGG RATRTLLRGG MSLRGQNLLR GGRAVAPRMG
160 170 180 190 200
LRRGGVRGRG GPGRGGLGRG AMGRGGIGGR GRGMIGRGRG GFGGRGRGRG
210 220 230 240
RGRGALARPV LTKEQLDNQL DAYMSKTKGH LDAELDAYMA QTDPETND
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketX6R700 | X6R700_HUMAN | Chromatin target of PRMT1 protein | CHTOP | 223 | Annotation score: | ||
Q5T7Y7 | Q5T7Y7_HUMAN | Chromatin target of PRMT1 protein | CHTOP | 101 | Annotation score: | ||
A0A087X1B7 | A0A087X1B7_HUMAN | Chromatin target of PRMT1 protein | CHTOP | 76 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 | K → N in AAH59949 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 180 | R → P in CAB43362 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 238 | Y → H in CAB43362 (PubMed:17974005).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017277 | 73 | Q → QK in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_040496 | 134 – 179 | Missing in isoform 3. CuratedAdd BLAST | 46 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF261137 mRNA Translation: AAG44673.1 AF318362 mRNA Translation: AAL55869.1 Frameshift. AL162258 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW53290.1 CH471121 Genomic DNA Translation: EAW53295.1 BC002733 mRNA Translation: AAH02733.1 Different initiation. BC059949 mRNA Translation: AAH59949.1 BC070027 mRNA Translation: AAH70027.1 Different initiation. BC108721 mRNA Translation: AAI08722.1 BC120961 mRNA Translation: AAI20962.1 BC120962 mRNA Translation: AAI20963.1 AL050260 mRNA Translation: CAB43362.2 AL512704 mRNA Translation: CAC21650.2 |
CCDSi | CCDS1048.1 [Q9Y3Y2-1] CCDS72917.1 [Q9Y3Y2-3] CCDS81380.1 [Q9Y3Y2-4] |
PIRi | T08660 |
RefSeqi | NP_001193541.1, NM_001206612.1 [Q9Y3Y2-3] NP_001231593.1, NM_001244664.1 NP_001304006.1, NM_001317077.1 [Q9Y3Y2-4] NP_056422.2, NM_015607.3 [Q9Y3Y2-1] |
Genome annotation databases
Ensembli | ENST00000368690; ENSP00000357679; ENSG00000160679 [Q9Y3Y2-3] ENST00000368694; ENSP00000357683; ENSG00000160679 ENST00000403433; ENSP00000385228; ENSG00000160679 [Q9Y3Y2-4] |
GeneIDi | 26097 |
KEGGi | hsa:26097 |
MANE-Selecti | ENST00000368694.8; ENSP00000357683.3; NM_015607.4; NP_056422.2 |
UCSCi | uc001fcm.3, human [Q9Y3Y2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF261137 mRNA Translation: AAG44673.1 AF318362 mRNA Translation: AAL55869.1 Frameshift. AL162258 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW53290.1 CH471121 Genomic DNA Translation: EAW53295.1 BC002733 mRNA Translation: AAH02733.1 Different initiation. BC059949 mRNA Translation: AAH59949.1 BC070027 mRNA Translation: AAH70027.1 Different initiation. BC108721 mRNA Translation: AAI08722.1 BC120961 mRNA Translation: AAI20962.1 BC120962 mRNA Translation: AAI20963.1 AL050260 mRNA Translation: CAB43362.2 AL512704 mRNA Translation: CAC21650.2 |
CCDSi | CCDS1048.1 [Q9Y3Y2-1] CCDS72917.1 [Q9Y3Y2-3] CCDS81380.1 [Q9Y3Y2-4] |
PIRi | T08660 |
RefSeqi | NP_001193541.1, NM_001206612.1 [Q9Y3Y2-3] NP_001231593.1, NM_001244664.1 NP_001304006.1, NM_001317077.1 [Q9Y3Y2-4] NP_056422.2, NM_015607.3 [Q9Y3Y2-1] |
3D structure databases
SMRi | Q9Y3Y2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 117547, 153 interactors |
CORUMi | Q9Y3Y2 |
IntActi | Q9Y3Y2, 76 interactors |
MINTi | Q9Y3Y2 |
STRINGi | 9606.ENSP00000357679 |
PTM databases
iPTMneti | Q9Y3Y2 |
MetOSitei | Q9Y3Y2 |
PhosphoSitePlusi | Q9Y3Y2 |
Genetic variation databases
BioMutai | CHTOP |
Proteomic databases
EPDi | Q9Y3Y2 |
jPOSTi | Q9Y3Y2 |
MassIVEi | Q9Y3Y2 |
MaxQBi | Q9Y3Y2 |
PaxDbi | Q9Y3Y2 |
PeptideAtlasi | Q9Y3Y2 |
PRIDEi | Q9Y3Y2 |
ProteomicsDBi | 86084 [Q9Y3Y2-1] 86085 [Q9Y3Y2-3] 86086 [Q9Y3Y2-4] |
Protocols and materials databases
Antibodypediai | 34133, 147 antibodies from 25 providers |
DNASUi | 26097 |
Genome annotation databases
Ensembli | ENST00000368690; ENSP00000357679; ENSG00000160679 [Q9Y3Y2-3] ENST00000368694; ENSP00000357683; ENSG00000160679 ENST00000403433; ENSP00000385228; ENSG00000160679 [Q9Y3Y2-4] |
GeneIDi | 26097 |
KEGGi | hsa:26097 |
MANE-Selecti | ENST00000368694.8; ENSP00000357683.3; NM_015607.4; NP_056422.2 |
UCSCi | uc001fcm.3, human [Q9Y3Y2-1] |
Organism-specific databases
CTDi | 26097 |
DisGeNETi | 26097 |
GeneCardsi | CHTOP |
HGNCi | HGNC:24511, CHTOP |
HPAi | ENSG00000160679, Low tissue specificity |
MIMi | 614206, gene |
neXtProti | NX_Q9Y3Y2 |
OpenTargetsi | ENSG00000160679 |
PharmGKBi | PA142672526 |
VEuPathDBi | HostDB:ENSG00000160679 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QV0X, Eukaryota |
GeneTreei | ENSGT00390000002869 |
HOGENOMi | CLU_087638_0_0_1 |
InParanoidi | Q9Y3Y2 |
OMAi | RFTNMMK |
OrthoDBi | 1598721at2759 |
PhylomeDBi | Q9Y3Y2 |
TreeFami | TF331447 |
Enzyme and pathway databases
PathwayCommonsi | Q9Y3Y2 |
Reactomei | R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination |
SignaLinki | Q9Y3Y2 |
Miscellaneous databases
BioGRID-ORCSi | 26097, 146 hits in 1057 CRISPR screens |
ChiTaRSi | CHTOP, human |
GenomeRNAii | 26097 |
Pharosi | Q9Y3Y2, Tbio |
PROi | PR:Q9Y3Y2 |
RNActi | Q9Y3Y2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000160679, Expressed in tendon of biceps brachii and 245 other tissues |
ExpressionAtlasi | Q9Y3Y2, baseline and differential |
Genevisiblei | Q9Y3Y2, HS |
Family and domain databases
InterProi | View protein in InterPro IPR025715, FoP_C |
Pfami | View protein in Pfam PF13865, FoP_duplication, 1 hit |
SMARTi | View protein in SMART SM01218, FoP_duplication, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHTOP_HUMAN | |
Accessioni | Q9Y3Y2Primary (citable) accession number: Q9Y3Y2 Secondary accession number(s): D3DV55 Q9H2L0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 23, 2022 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot