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Protein

Signaling threshold-regulating transmembrane adapter 1

Gene

SIT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.1 Publication

Caution

In contrast to its orthologs it harbors a signal sequence.Curated

GO - Molecular functioni

  • kinase binding Source: UniProtKB
  • SH2 domain binding Source: HGNC

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • regulation of T cell activation Source: UniProtKB
  • signal transduction Source: ProtInc
  • T cell homeostasis Source: InterPro

Keywordsi

Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

SignaLinkiQ9Y3P8

Names & Taxonomyi

Protein namesi
Recommended name:
Signaling threshold-regulating transmembrane adapter 1
Alternative name(s):
SHP2-interacting transmembrane adapter protein
Suppression-inducing transmembrane adapter 1
gp30/40
Gene namesi
Name:SIT1
Synonyms:SIT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000137078.8
HGNCiHGNC:17710 SIT1
MIMi604964 gene
neXtProtiNX_Q9Y3P8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 40ExtracellularSequence analysisAdd BLAST16
Transmembranei41 – 61HelicalSequence analysisAdd BLAST21
Topological domaini62 – 196CytoplasmicSequence analysisAdd BLAST135

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26N → Q: Abolishes glycosylation. 1 Publication1
Mutagenesisi90Y → F: Reduces interaction with GRB2. Abolishes interaction with GRB2; when associated with F-188. 1 Publication1
Mutagenesisi127Y → F: No effect on interaction with PTPN11 or GRB2. 1 Publication1
Mutagenesisi148Y → F: Reduces interaction with PTPN11, no effect on inhibition of NF-AT activation. 2 Publications1
Mutagenesisi169Y → F: Abolishes interaction with CSK and impairs inhibition of NF-AT activation. 1 Publication1
Mutagenesisi188Y → F: Reduces interaction with GRB2. Abolishes interaction with GRB2; when associated with F-90. 1 Publication1

Organism-specific databases

DisGeNETi27240
OpenTargetsiENSG00000137078
PharmGKBiPA142670914

Polymorphism and mutation databases

BioMutaiSIT1
DMDMi74753488

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000004515225 – 196Signaling threshold-regulating transmembrane adapter 1Add BLAST172

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi26N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi27InterchainCurated
Modified residuei80PhosphoserineCombined sources1
Modified residuei83PhosphoserineBy similarity1
Modified residuei90PhosphotyrosineCombined sources1 Publication1
Modified residuei102PhosphoserineCombined sources1
Modified residuei127Phosphotyrosine1 Publication1
Modified residuei144PhosphothreonineCombined sources1
Modified residuei148PhosphotyrosineCombined sources2 Publications1
Modified residuei169Phosphotyrosine1 Publication1
Modified residuei182PhosphoserineCombined sources1
Modified residuei188PhosphotyrosineCombined sources1 Publication1

Post-translational modificationi

Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9Y3P8
MaxQBiQ9Y3P8
PaxDbiQ9Y3P8
PeptideAtlasiQ9Y3P8
PRIDEiQ9Y3P8
ProteomicsDBi86053

PTM databases

iPTMnetiQ9Y3P8
PhosphoSitePlusiQ9Y3P8

Expressioni

Tissue specificityi

Specifically expressed in T- and B-cells. Present in plasma cells but not in germinal center B-cells (at protein level). Expressed in T- and B-cell lymphoma.2 Publications

Gene expression databases

BgeeiENSG00000137078
CleanExiHS_SIT1
ExpressionAtlasiQ9Y3P8 baseline and differential
GenevisibleiQ9Y3P8 HS

Organism-specific databases

HPAiHPA018506

Interactioni

Subunit structurei

Homodimer; disulfide-linked. When phosphorylated, interacts with PTPN11/SHP2, GRB2 and CSK.2 Publications

GO - Molecular functioni

  • kinase binding Source: UniProtKB
  • SH2 domain binding Source: HGNC

Protein-protein interaction databases

BioGridi118088, 5 interactors
IntActiQ9Y3P8, 1 interactor
MINTiQ9Y3P8
STRINGi9606.ENSP00000259608

Structurei

3D structure databases

ProteinModelPortaliQ9Y3P8
SMRiQ9Y3P8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 93Interaction with GRB21 Publication4
Regioni146 – 151Interaction with PTPN116
Regioni169 – 172Interaction with CSK1 Publication4
Regioni188 – 191Interaction with GRB21 Publication4

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYYR Eukaryota
ENOG4111C3Q LUCA
GeneTreeiENSGT00390000016476
HOGENOMiHOG000231524
HOVERGENiHBG080311
InParanoidiQ9Y3P8
PhylomeDBiQ9Y3P8
TreeFamiTF337816

Family and domain databases

InterProiView protein in InterPro
IPR033269 Sit1
PANTHERiPTHR15604 PTHR15604, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD358400 PD358400, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y3P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQADPRLRA VCLWTLTSAA MSRGDNCTDL LALGIPSITQ AWGLWVLLGA
60 70 80 90 100
VTLLFLISLA AHLSQWTRGR SRSHPGQGRS GESVEEVPLY GNLHYLQTGR
110 120 130 140 150
LSQDPEPDQQ DPTLGGPARA AEEVMCYTSL QLRPPQGRIP GPGTPVKYSE
160 170 180 190
VVLDSEPKSQ ASGPEPELYA SVCAQTRRAR ASFPDQAYAN SQPAAS
Length:196
Mass (Da):21,126
Last modified:November 1, 1999 - v1
Checksum:i2A0C48C9466F7F07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010059 mRNA Translation: CAB41504.1
AJ271888 Genomic DNA Translation: CAC81313.1
AK314758 mRNA Translation: BAG37296.1
AL357874 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58370.1
BC102029 mRNA Translation: AAI02030.1
BC104491 mRNA Translation: AAI04492.1
BC107484 mRNA Translation: AAI07485.1
CCDSiCCDS6582.1
RefSeqiNP_055265.1, NM_014450.2
UniGeneiHs.88012

Genome annotation databases

EnsembliENST00000259608; ENSP00000259608; ENSG00000137078
GeneIDi27240
KEGGihsa:27240
UCSCiuc003zxe.3 human

Similar proteinsi

Entry informationi

Entry nameiSIT1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3P8
Secondary accession number(s): B2RBP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1999
Last modified: June 20, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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