UniProtKB - Q9Y3L3 (3BP1_HUMAN)
SH3 domain-binding protein 1
SH3BP1
Functioni
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB
- Rac GTPase binding Source: GO_Central
- semaphorin receptor binding Source: UniProtKB
- SH3 domain binding Source: UniProtKB-KW
GO - Biological processi
- actin filament organization Source: UniProtKB
- cell junction assembly Source: UniProtKB
- cell migration Source: UniProtKB
- establishment of epithelial cell apical/basal polarity Source: UniProtKB
- negative regulation of small GTPase mediated signal transduction Source: UniProtKB
- phagocytosis, engulfment Source: UniProtKB
- positive regulation of GTPase activity Source: UniProtKB
- regulation of actin cytoskeleton organization Source: UniProtKB
- regulation of actin filament depolymerization Source: UniProtKB
- regulation of blood vessel endothelial cell migration Source: UniProtKB
- regulation of Rac protein signal transduction Source: GO_Central
- semaphorin-plexin signaling pathway Source: UniProtKB
Keywordsi
Molecular function | GTPase activation |
Biological process | Phagocytosis |
Enzyme and pathway databases
PathwayCommonsi | Q9Y3L3 |
SIGNORi | Q9Y3L3 |
Names & Taxonomyi
Protein namesi | Recommended name: SH3 domain-binding protein 1Imported |
Gene namesi | Name:SH3BP1Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10824, SH3BP1 |
MIMi | 617368, gene |
neXtProti | NX_Q9Y3L3 |
VEuPathDBi | HostDB:ENSG00000100092.20 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol 1 Publication
Other locations
- Cell projection 2 Publications
- tight junction 1 Publication
- adherens junction 1 Publication
- phagocytic cup 1 Publication
Note: Localizes at the leading edge of migrating cells (PubMed:21658605, PubMed:24841563). Accumulation at forming phagocytic cups is PI3 kinase/PI3K-dependent and is specific for sites of large particles engagement and their phosphatidylinositol 3,4,5-triphosphate membrane content (PubMed:26465210).3 Publications
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- phagocytic cup Source: UniProtKB
Other locations
- adherens junction Source: UniProtKB
- bicellular tight junction Source: UniProtKB
- cell leading edge Source: UniProtKB
- lamellipodium Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell projection, Cytoplasm, Nucleus, Tight junctionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 312 | R → A: Probable loss of the GTPase activator activity. Loss of function in cell migration. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 23616 |
OpenTargetsi | ENSG00000100092 |
PharmGKBi | PA35732 |
Miscellaneous databases
Pharosi | Q9Y3L3, Tbio |
Genetic variation databases
BioMutai | SH3BP1 |
DMDMi | 51338841 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056723 | 1 – 701 | SH3 domain-binding protein 1Add BLAST | 701 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 175 | PhosphoserineCombined sources | 1 | |
Modified residuei | 241 | PhosphoserineBy similarity | 1 | |
Modified residuei | 262 | PhosphoserineCombined sources | 1 | |
Modified residuei | 264 | PhosphoserineCombined sources | 1 | |
Modified residuei | 544 | PhosphoserineCombined sources | 1 | |
Modified residuei | 550 | PhosphoserineCombined sources | 1 | |
Modified residuei | 601 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 626 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 653 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
CPTACi | CPTAC-1004 |
EPDi | Q9Y3L3 |
jPOSTi | Q9Y3L3 |
MassIVEi | Q9Y3L3 |
MaxQBi | Q9Y3L3 |
PaxDbi | Q9Y3L3 |
PeptideAtlasi | Q9Y3L3 |
PRIDEi | Q9Y3L3 |
ProteomicsDBi | 86041 [Q9Y3L3-1] 86042 [Q9Y3L3-2] |
PTM databases
iPTMneti | Q9Y3L3 |
PhosphoSitePlusi | Q9Y3L3 |
Expressioni
Gene expression databases
Bgeei | ENSG00000100092, Expressed in granulocyte and 140 other tissues |
ExpressionAtlasi | Q9Y3L3, baseline and differential |
Genevisiblei | Q9Y3L3, HS |
Organism-specific databases
HPAi | ENSG00000100092, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with RAC1 (By similarity).
Interacts with the exocyst via EXOC4 and EXOC8; required for the localization of both SH3BP1 and the exocyst to the leading edge of migrating cells (PubMed:21658605).
Interacts with CD2AP and CGNL1; probably part of a complex at cell junctions (PubMed:22891260).
Interacts with CAPZA1; recruits CAPZA1 to forming cell junctions (PubMed:22891260). May interact with AFDN (PubMed:22891260).
Interacts with PLXND1; they dissociate upon SEMA3E binding to PLXND1 allowing SH3BP1 to transduce downstream signal through RAC1 inactivation (PubMed:24841563).
Interacts with ABL1, GRB2 and SRC (via SH3 domain) (By similarity).
By similarity3 PublicationsBinary interactionsi
Hide detailsQ9Y3L3
GO - Molecular functioni
- Rac GTPase binding Source: GO_Central
- semaphorin receptor binding Source: UniProtKB
- SH3 domain binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 117149, 25 interactors |
IntActi | Q9Y3L3, 19 interactors |
MINTi | Q9Y3L3 |
STRINGi | 9606.ENSP00000350018 |
Miscellaneous databases
RNActi | Q9Y3L3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 262 | BARPROSITE-ProRule annotationAdd BLAST | 246 | |
Domaini | 276 – 469 | Rho-GAPPROSITE-ProRule annotationAdd BLAST | 194 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 275 | Interaction with CGNL11 PublicationAdd BLAST | 275 | |
Regioni | 470 – 701 | Interaction with CD2AP1 PublicationAdd BLAST | 232 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 616 – 625 | SH3-bindingBy similarity | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 500 – 693 | Pro-richPROSITE-ProRule annotationAdd BLAST | 194 |
Domaini
Keywords - Domaini
SH3-bindingPhylogenomic databases
eggNOGi | KOG4270, Eukaryota |
GeneTreei | ENSGT00940000158369 |
HOGENOMi | CLU_013806_2_0_1 |
InParanoidi | Q9Y3L3 |
OMAi | QGCMQSQ |
OrthoDBi | 821331at2759 |
PhylomeDBi | Q9Y3L3 |
TreeFami | TF316514 |
Family and domain databases
Gene3Di | 1.10.555.10, 1 hit 1.20.1270.60, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR004148, BAR_dom IPR008936, Rho_GTPase_activation_prot IPR000198, RhoGAP_dom |
Pfami | View protein in Pfam PF03114, BAR, 1 hit PF00620, RhoGAP, 1 hit |
SMARTi | View protein in SMART SM00721, BAR, 1 hit SM00324, RhoGAP, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF48350, SSF48350, 1 hit |
PROSITEi | View protein in PROSITE PS51021, BAR, 1 hit PS50238, RHOGAP, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing and alternative initiation. AlignAdd to basketThis entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMKRQLHRMR QLAQTGSLGR TPETAEFLGE DLLQVEQRLE PAKRAAHNIH
60 70 80 90 100
KRLQACLQGQ SGADMDKRVK KLPLMALSTT MAESFKELDP DSSMGKALEM
110 120 130 140 150
SCAIQNQLAR ILAEFEMTLE RDVLQPLSRL SEEELPAILK HKKSLQKLVS
160 170 180 190 200
DWNTLKSRLS QATKNSGSSQ GLGGSPGSHS HTTMANKVET LKEEEEELKR
210 220 230 240 250
KVEQCRDEYL ADLYHFVTKE DSYANYFIRL LEIQADYHRR SLSSLDTALA
260 270 280 290 300
ELRENHGQAD HSPSMTATHF PRVYGVSLAT HLQELGREIA LPIEACVMML
310 320 330 340 350
LSEGMKEEGL FRLAAGASVL KRLKQTMASD PHSLEEFCSD PHAVAGALKS
360 370 380 390 400
YLRELPEPLM TFDLYDDWMR AASLKEPGAR LQALQEVCSR LPPENLSNLR
410 420 430 440 450
YLMKFLARLA EEQEVNKMTP SNIAIVLGPN LLWPPEKEGD QAQLDAASVS
460 470 480 490 500
SIQVVGVVEA LIQSADTLFP GDINFNVSGL FSAVTLQDTV SDRLASEELP
510 520 530 540 550
STAVPTPATT PAPAPAPAPA PAPALASAAT KERTESEVPP RPASPKVTRS
560 570 580 590 600
PPETAAPVED MARRTKRPAP ARPTMPPPQV SGSRSSPPAP PLPPGSGSPG
610 620 630 640 650
TPQALPRRLV GSSLRAPTVP PPLPPTPPQP ARRQSRRSPA SPSPASPGPA
660 670 680 690 700
SPSPVSLSNP AQVDLGAATA EGGAPEAISG VPTPPAIPPQ PRPRSLASET
N
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A2R8Y5V0 | A0A2R8Y5V0_HUMAN | SH3 domain-binding protein 1 | SH3BP1 | 387 | Annotation score: | ||
A0A3B3IU28 | A0A3B3IU28_HUMAN | SH3 domain-binding protein 1 | SH3BP1 | 206 | Annotation score: | ||
F8WEQ3 | F8WEQ3_HUMAN | SH3 domain-binding protein 1 | SH3BP1 | 20 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_033450 | 511 | P → L. Corresponds to variant dbSNP:rs929038Ensembl. | 1 | |
Natural variantiVAR_033451 | 656 | S → F. Corresponds to variant dbSNP:rs2269548Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011373 | 440 – 452 | DQAQL…SVSSI → TEPARELGSQTLC in isoform 2. 1 PublicationAdd BLAST | 13 | |
Alternative sequenceiVSP_011374 | 453 – 701 | Missing in isoform 2. 1 PublicationAdd BLAST | 249 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR456576 mRNA Translation: CAG30462.1 AK124370 mRNA Translation: BAC85842.1 Sequence problems. Z83844 Genomic DNA No translation available. BC008282 mRNA Translation: AAH08282.1 Different initiation. AL157480 mRNA Translation: CAB75671.2 |
CCDSi | CCDS13952.2 [Q9Y3L3-1] |
PIRi | T46916 |
RefSeqi | NP_061830.3, NM_018957.3 [Q9Y3L3-1] |
Genome annotation databases
Ensembli | ENST00000417536; ENSP00000411979; ENSG00000100092 [Q9Y3L3-2] ENST00000649765; ENSP00000497104; ENSG00000100092 [Q9Y3L3-1] |
GeneIDi | 23616 |
KEGGi | hsa:23616 |
UCSCi | uc003atg.2, human [Q9Y3L3-1] |
Keywords - Coding sequence diversityi
Alternative initiation, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR456576 mRNA Translation: CAG30462.1 AK124370 mRNA Translation: BAC85842.1 Sequence problems. Z83844 Genomic DNA No translation available. BC008282 mRNA Translation: AAH08282.1 Different initiation. AL157480 mRNA Translation: CAB75671.2 |
CCDSi | CCDS13952.2 [Q9Y3L3-1] |
PIRi | T46916 |
RefSeqi | NP_061830.3, NM_018957.3 [Q9Y3L3-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4J9D | X-ray | 1.50 | B/D/F | 616-625 | [»] | |
4J9F | X-ray | 1.09 | B/D/F | 616-625 | [»] | |
SMRi | Q9Y3L3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117149, 25 interactors |
IntActi | Q9Y3L3, 19 interactors |
MINTi | Q9Y3L3 |
STRINGi | 9606.ENSP00000350018 |
PTM databases
iPTMneti | Q9Y3L3 |
PhosphoSitePlusi | Q9Y3L3 |
Genetic variation databases
BioMutai | SH3BP1 |
DMDMi | 51338841 |
Proteomic databases
CPTACi | CPTAC-1004 |
EPDi | Q9Y3L3 |
jPOSTi | Q9Y3L3 |
MassIVEi | Q9Y3L3 |
MaxQBi | Q9Y3L3 |
PaxDbi | Q9Y3L3 |
PeptideAtlasi | Q9Y3L3 |
PRIDEi | Q9Y3L3 |
ProteomicsDBi | 86041 [Q9Y3L3-1] 86042 [Q9Y3L3-2] |
Protocols and materials databases
Antibodypediai | 214, 223 antibodies |
DNASUi | 23616 |
Genome annotation databases
Ensembli | ENST00000417536; ENSP00000411979; ENSG00000100092 [Q9Y3L3-2] ENST00000649765; ENSP00000497104; ENSG00000100092 [Q9Y3L3-1] |
GeneIDi | 23616 |
KEGGi | hsa:23616 |
UCSCi | uc003atg.2, human [Q9Y3L3-1] |
Organism-specific databases
CTDi | 23616 |
DisGeNETi | 23616 |
GeneCardsi | SH3BP1 |
HGNCi | HGNC:10824, SH3BP1 |
HPAi | ENSG00000100092, Low tissue specificity |
MIMi | 617368, gene |
neXtProti | NX_Q9Y3L3 |
OpenTargetsi | ENSG00000100092 |
PharmGKBi | PA35732 |
VEuPathDBi | HostDB:ENSG00000100092.20 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4270, Eukaryota |
GeneTreei | ENSGT00940000158369 |
HOGENOMi | CLU_013806_2_0_1 |
InParanoidi | Q9Y3L3 |
OMAi | QGCMQSQ |
OrthoDBi | 821331at2759 |
PhylomeDBi | Q9Y3L3 |
TreeFami | TF316514 |
Enzyme and pathway databases
PathwayCommonsi | Q9Y3L3 |
SIGNORi | Q9Y3L3 |
Miscellaneous databases
BioGRID-ORCSi | 23616, 12 hits in 871 CRISPR screens |
ChiTaRSi | SH3BP1, human |
GeneWikii | SH3BP1 |
GenomeRNAii | 23616 |
Pharosi | Q9Y3L3, Tbio |
PROi | PR:Q9Y3L3 |
RNActi | Q9Y3L3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000100092, Expressed in granulocyte and 140 other tissues |
ExpressionAtlasi | Q9Y3L3, baseline and differential |
Genevisiblei | Q9Y3L3, HS |
Family and domain databases
Gene3Di | 1.10.555.10, 1 hit 1.20.1270.60, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR004148, BAR_dom IPR008936, Rho_GTPase_activation_prot IPR000198, RhoGAP_dom |
Pfami | View protein in Pfam PF03114, BAR, 1 hit PF00620, RhoGAP, 1 hit |
SMARTi | View protein in SMART SM00721, BAR, 1 hit SM00324, RhoGAP, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF48350, SSF48350, 1 hit |
PROSITEi | View protein in PROSITE PS51021, BAR, 1 hit PS50238, RHOGAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 3BP1_HUMAN | |
Accessioni | Q9Y3L3Primary (citable) accession number: Q9Y3L3 Secondary accession number(s): Q5R3N0 Q9NSQ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2002 |
Last sequence update: | August 16, 2004 | |
Last modified: | February 10, 2021 | |
This is version 182 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants