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Protein

Adenylate kinase isoenzyme 6

Gene

AK6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. AMP and dAMP are the preferred substrates, but CMP and dCMP are also good substrates. IMP is phosphorylated to a much lesser extent. All nucleoside triphosphates ATP, GTP, UTP, CTP, dATP, dCTP, dGTP, and TTP are accepted as phosphate donors. CTP is the best phosphate donor, followed by UTP, ATP, GTP and dCTP. May have a role in nuclear energy homeostasis. Has also ATPase activity. May be involved in regulation of Cajal body (CB) formation.1 Publication

Caution

AK6 and TAF9 were initially considered as products of the same gene since they share two exons. However, they are translated from different initiation codons and reading frames and encode unrelated proteins. This arrangement is conserved in some mammalian species.Curated

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation1 Publication

Kineticsi

kcat is 6.3 sec(-1) for adenylate kinase activity. kcat is 4.8 sec(-1) for ATPase activity.
  1. KM=192 µM for AMP3 Publications
  2. KM=45 µM for ATP (for adenylate kinase activity)3 Publications
  3. KM=332 µM for ATP (for ATPase activity)3 Publications
  1. Vmax=1.27 µmol/min/mg enzyme for ATPase activity3 Publications
  2. Vmax=982 nmol/min/mg enzyme for the forward adenylate kinase reaction3 Publications
  3. Vmax=955 nmol/min/mg enzyme for the reverse adenylate kinase reaction3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39AMPCurated1
Binding sitei79ATP1
Binding sitei105ATP1
Binding sitei109ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 18ATP6
Nucleotide bindingi148 – 149ATP2

GO - Molecular functioni

  • adenylate kinase activity Source: UniProtKB
  • ATP binding Source: GO_Central
  • nucleoside monophosphate kinase activity Source: Reactome

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.4.3 2681
ReactomeiR-HSA-499943 Interconversion of nucleotide di- and triphosphates

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 6UniRule annotation (EC:2.7.4.3UniRule annotation)
Short name:
AK6UniRule annotation
Alternative name(s):
Adrenal gland protein AD-004
Coilin-interacting nuclear ATPase proteinUniRule annotation
Short name:
hCINAP
Dual activity adenylate kinase/ATPaseUniRule annotation
Short name:
AK/ATPaseUniRule annotation
Gene namesi
Name:AK6UniRule annotation
Synonyms:CINAPUniRule annotation
ORF Names:AD-004, CGI-137
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000085231.13
HGNCiHGNC:49151 AK6
neXtProtiNX_Q9Y3D8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi79H → G: Induces homodimerization. Reduces adenylate kinase activity by 72% and ATPase activity by 76%. Significantly changes Cajal body organization in the nucleus, resulting in enhanced apoptosis and reduced proliferation. 1 Publication1

Organism-specific databases

DisGeNETi102157402
OpenTargetsiENSG00000085231
PharmGKBiPA36317

Polymorphism and mutation databases

BioMutaiCCDC125
DMDMi6831735

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001538961 – 172Adenylate kinase isoenzyme 6Add BLAST172

Proteomic databases

EPDiQ9Y3D8
PeptideAtlasiQ9Y3D8
PRIDEiQ9Y3D8
ProteomicsDBi86024
86025 [Q9Y3D8-2]

PTM databases

iPTMnetiQ9Y3D8
PhosphoSitePlusiQ9Y3D8

Expressioni

Tissue specificityi

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, chorionic villi and the central nervous system.1 Publication

Gene expression databases

BgeeiENSG00000085231
ExpressionAtlasiQ9Y3D8 baseline and differential
GenevisibleiQ9Y3D8 HS

Organism-specific databases

HPAiCAB005361
HPA034935

Interactioni

Subunit structurei

Monomer and homodimer (By similarity). Interacts with COIL (via C-terminus).UniRule annotation3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi3194050, 5 interactors
IntActiQ9Y3D8, 8 interactors
MINTiQ9Y3D8

Structurei

Secondary structure

1172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Helixi16 – 27Combined sources12
Beta strandi30 – 33Combined sources4
Helixi34 – 41Combined sources8
Beta strandi44 – 48Combined sources5
Turni49 – 52Combined sources4
Beta strandi53 – 56Combined sources4
Helixi58 – 71Combined sources14
Beta strandi74 – 77Combined sources4
Helixi86 – 88Combined sources3
Beta strandi90 – 96Combined sources7
Helixi99 – 108Combined sources10
Helixi113 – 124Combined sources12
Helixi127 – 135Combined sources9
Helixi138 – 140Combined sources3
Beta strandi141 – 145Combined sources5
Helixi149 – 169Combined sources21

3D structure databases

ProteinModelPortaliQ9Y3D8
SMRiQ9Y3D8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y3D8

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 56NMPbindAdd BLAST24
Regioni108 – 118LIDAdd BLAST11

Sequence similaritiesi

Belongs to the adenylate kinase family. AK6 subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000015930
HOGENOMiHOG000224472
HOVERGENiHBG052207
KOiK18532
OMAiDNVQCEI
OrthoDBiEOG091G0RS8

Family and domain databases

HAMAPiMF_00039 Adenylate_kinase_AK6, 1 hit
InterProiView protein in InterPro
IPR020618 Adenyl_kinase_AK6
IPR027417 P-loop_NTPase
PANTHERiPTHR12595:SF0 PTHR12595:SF0, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y3D8-1) [UniParc]FASTAAdd to basket
Also known as: AK6

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLPNILLTG TPGVGKTTLG KELASKSGLK YINVGDLARE EQLYDGYDEE
60 70 80 90 100
YDCPILDEDR VVDELDNQMR EGGVIVDYHG CDFFPERWFH IVFVLRTDTN
110 120 130 140 150
VLYERLETRG YNEKKLTDNI QCEIFQVLYE EATASYKEEI VHQLPSNKPE
160 170
ELENNVDQIL KWIEQWIKDH NS
Length:172
Mass (Da):20,061
Last modified:November 1, 1999 - v1
Checksum:i33F62318FB434301
GO
Isoform 2 (identifier: Q9Y3D8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-9: LLPNILLT → CHRKP

Note: Gene prediction based on EST data.
Show »
Length:169
Mass (Da):19,805
Checksum:i3F341AE6C5898C66
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0397142 – 9LLPNILLT → CHRKP in isoform 2. Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ878880 mRNA Translation: CAI48030.1
AJ878881 mRNA Translation: CAI48031.1
AF151895 mRNA Translation: AAD34132.1
AF110777 mRNA Translation: AAF14860.1
AC145132 Genomic DNA No translation available.
CH471137 Genomic DNA Translation: EAW51290.1
CH471137 Genomic DNA Translation: EAW51291.1
CH471137 Genomic DNA Translation: EAW51292.1
CH471137 Genomic DNA Translation: EAW51297.1
BC007349 mRNA Translation: AAH07349.1
BC007426 mRNA Translation: AAH07426.1
CCDSiCCDS4001.1 [Q9Y3D8-1]
CCDS43324.1 [Q9Y3D8-2]
RefSeqiNP_001015891.1, NM_001015891.1 [Q9Y3D8-2]
NP_057367.1, NM_016283.4 [Q9Y3D8-1]
UniGeneiHs.653163

Genome annotation databases

EnsembliENST00000380818; ENSP00000370197; ENSG00000085231 [Q9Y3D8-2]
ENST00000380822; ENSP00000370201; ENSG00000085231 [Q9Y3D8-1]
ENST00000612207; ENSP00000484679; ENSG00000279247 [Q9Y3D8-1]
ENST00000620269; ENSP00000480829; ENSG00000279247 [Q9Y3D8-2]
GeneIDi102157402
KEGGihsa:102157402
UCSCiuc003jwa.4 human [Q9Y3D8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKAD6_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3D8
Secondary accession number(s): A8MSZ6, Q5F2S9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: July 18, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

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