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Protein

WASH complex subunit 3

Gene

WASHC3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093).2 Publications

Caution

The function of the WASH complex is debated. One study using partially purified samples reported a nucleation-promoting factor (NPF) activity (PubMed:19922875). In another study, the reconstituted and highly purified recombinant WASH core complex did not show activity toward Arp2/3 complex suggesting a rather inhibitory role towards WASH NPF activity (PubMed:20498093).

GO - Biological processi

  • actin filament polymerization Source: GO_Central
  • exocytosis Source: GO_Central
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transport

Protein family/group databases

TCDBi9.A.3.1.2 the sorting nexin27 (snx27)-retromer assembly apparatus for recycling integral membrane proteins (snx27-retromeraa) family

Names & Taxonomyi

Protein namesi
Recommended name:
WASH complex subunit 3Imported
Alternative name(s):
Coiled-coil domain-containing protein 53
Gene namesi
Name:WASHC3Imported
ORF Names:AD-016, CCDC53, CGI-116, x0009
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000120860.10
HGNCiHGNC:24256 WASHC3
neXtProtiNX_Q9Y3C0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000120860
PharmGKBiPA142672169

Polymorphism and mutation databases

BioMutaiCCDC53
DMDMi6831732

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762011 – 194WASH complex subunit 3Add BLAST194

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9Y3C0
MaxQBiQ9Y3C0
PaxDbiQ9Y3C0
PeptideAtlasiQ9Y3C0
PRIDEiQ9Y3C0
ProteomicsDBi86009

PTM databases

iPTMnetiQ9Y3C0
PhosphoSitePlusiQ9Y3C0

Expressioni

Gene expression databases

BgeeiENSG00000120860 Expressed in 226 organ(s), highest expression level in right testis
CleanExiHS_CCDC53
ExpressionAtlasiQ9Y3C0 baseline and differential
GenevisibleiQ9Y3C0 HS

Organism-specific databases

HPAiHPA038338
HPA038339

Interactioni

Subunit structurei

Component of the WASH core complex also described as WASH regulatory complex (SHRC) composed of WASH (WASHC1, WASH2P or WASH3P), WASHC2 (WASHC2A or WASHC2C), WASHC3, WASHC4 and WASHC5. The WASH core complex associates via WASHC2 with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi119225, 77 interactors
ComplexPortaliCPX-1163 WASH complex, variant WASHC1/WASHC2C
CPX-1168 WASH complex, variant WASH2P/WASHC2C
CPX-1169 WASH complex, variant WASH3P/WASHC2C
CPX-1170 WASH complex, variant WASH4P/WASHC2C
CPX-1171 WASH complex, variant WASH6P/WASHC2C
CPX-1172 WASH complex, variant WASHC1/WASHC2A
CPX-1173 WASH complex, variant WASH2P/WASHC2A
CPX-1174 WASH complex, variant WASH3P/WASHC2A
CPX-1175 WASH complex, variant WASH4P/WASHC2A
CPX-1176 WASH complex, variant WASH6P/WASHC2A
CORUMiQ9Y3C0
IntActiQ9Y3C0, 62 interactors
MINTiQ9Y3C0
STRINGi9606.ENSP00000240079

Structurei

3D structure databases

ProteinModelPortaliQ9Y3C0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili46 – 74Sequence analysisAdd BLAST29

Sequence similaritiesi

Belongs to the CCDC53 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4496 Eukaryota
ENOG4111KKV LUCA
GeneTreeiENSGT00390000014084
HOGENOMiHOG000006526
HOVERGENiHBG009789
InParanoidiQ9Y3C0
KOiK18463
OMAiQKRILAF
OrthoDBiEOG091G0Y6M
PhylomeDBiQ9Y3C0
TreeFamiTF318955

Family and domain databases

InterProiView protein in InterPro
IPR019309 WASHC3
PANTHERiPTHR13015 PTHR13015, 2 hits
PfamiView protein in Pfam
PF10152 CCDC53, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

Q9Y3C0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDEDGLPLMG SGIDLTKVPA IQQKRTVAFL NQFVVHTVQF LNRFSTVCEE
60 70 80 90 100
KLADLSLRIQ QIETTLNILD AKLSSIPGLD DVTVEVSPLN VTSVTNGAHP
110 120 130 140 150
EATSEQPQQN STQDSGLQES EVSAENILTV AKDPRYARYL KMVQVGVPVM
160 170 180 190
AIRNKMISEG LDPDLLERPD APVPDGESEK TVEESSDSES SFSD
Length:194
Mass (Da):21,173
Last modified:November 1, 1999 - v1
Checksum:iB0CCF1AE76CDA6D2
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5GZ97F5GZ97_HUMAN
WASH complex subunit 3
WASHC3
193Annotation score:
F5GWI9F5GWI9_HUMAN
WASH complex subunit 3
WASHC3
169Annotation score:
F5GXZ7F5GXZ7_HUMAN
WASH complex subunit 3
WASHC3
173Annotation score:
H0YGN0H0YGN0_HUMAN
WASH complex subunit 3
WASHC3
107Annotation score:
F5H2P1F5H2P1_HUMAN
WASH complex subunit 3
WASHC3
77Annotation score:
F5H0A7F5H0A7_HUMAN
WASH complex subunit 3
WASHC3
50Annotation score:
F5GXZ4F5GXZ4_HUMAN
WASH complex subunit 3
WASHC3
61Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti81D → G in BAD97059 (Ref. 5) Curated1
Sequence conflicti110N → S in AAH10889 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155655 mRNA Translation: AAF67012.1
AF151874 mRNA Translation: AAD34111.1
CR457171 mRNA Translation: CAG33452.1
AK223339 mRNA Translation: BAD97059.1
AK314970 mRNA Translation: BAG37471.1
CH471054 Genomic DNA Translation: EAW97687.1
BC010889 mRNA Translation: AAH10889.1
CCDSiCCDS44959.1
RefSeqiNP_057137.1, NM_016053.3
UniGeneiHs.405692

Genome annotation databases

EnsembliENST00000240079; ENSP00000240079; ENSG00000120860
GeneIDi51019
KEGGihsa:51019
UCSCiuc010svw.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155655 mRNA Translation: AAF67012.1
AF151874 mRNA Translation: AAD34111.1
CR457171 mRNA Translation: CAG33452.1
AK223339 mRNA Translation: BAD97059.1
AK314970 mRNA Translation: BAG37471.1
CH471054 Genomic DNA Translation: EAW97687.1
BC010889 mRNA Translation: AAH10889.1
CCDSiCCDS44959.1
RefSeqiNP_057137.1, NM_016053.3
UniGeneiHs.405692

3D structure databases

ProteinModelPortaliQ9Y3C0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119225, 77 interactors
ComplexPortaliCPX-1163 WASH complex, variant WASHC1/WASHC2C
CPX-1168 WASH complex, variant WASH2P/WASHC2C
CPX-1169 WASH complex, variant WASH3P/WASHC2C
CPX-1170 WASH complex, variant WASH4P/WASHC2C
CPX-1171 WASH complex, variant WASH6P/WASHC2C
CPX-1172 WASH complex, variant WASHC1/WASHC2A
CPX-1173 WASH complex, variant WASH2P/WASHC2A
CPX-1174 WASH complex, variant WASH3P/WASHC2A
CPX-1175 WASH complex, variant WASH4P/WASHC2A
CPX-1176 WASH complex, variant WASH6P/WASHC2A
CORUMiQ9Y3C0
IntActiQ9Y3C0, 62 interactors
MINTiQ9Y3C0
STRINGi9606.ENSP00000240079

Protein family/group databases

TCDBi9.A.3.1.2 the sorting nexin27 (snx27)-retromer assembly apparatus for recycling integral membrane proteins (snx27-retromeraa) family

PTM databases

iPTMnetiQ9Y3C0
PhosphoSitePlusiQ9Y3C0

Polymorphism and mutation databases

BioMutaiCCDC53
DMDMi6831732

Proteomic databases

EPDiQ9Y3C0
MaxQBiQ9Y3C0
PaxDbiQ9Y3C0
PeptideAtlasiQ9Y3C0
PRIDEiQ9Y3C0
ProteomicsDBi86009

Protocols and materials databases

DNASUi51019
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000240079; ENSP00000240079; ENSG00000120860
GeneIDi51019
KEGGihsa:51019
UCSCiuc010svw.3 human

Organism-specific databases

CTDi51019
EuPathDBiHostDB:ENSG00000120860.10
GeneCardsiWASHC3
HGNCiHGNC:24256 WASHC3
HPAiHPA038338
HPA038339
neXtProtiNX_Q9Y3C0
OpenTargetsiENSG00000120860
PharmGKBiPA142672169
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4496 Eukaryota
ENOG4111KKV LUCA
GeneTreeiENSGT00390000014084
HOGENOMiHOG000006526
HOVERGENiHBG009789
InParanoidiQ9Y3C0
KOiK18463
OMAiQKRILAF
OrthoDBiEOG091G0Y6M
PhylomeDBiQ9Y3C0
TreeFamiTF318955

Miscellaneous databases

ChiTaRSiCCDC53 human
GeneWikiiCCDC53
GenomeRNAii51019
PROiPR:Q9Y3C0

Gene expression databases

BgeeiENSG00000120860 Expressed in 226 organ(s), highest expression level in right testis
CleanExiHS_CCDC53
ExpressionAtlasiQ9Y3C0 baseline and differential
GenevisibleiQ9Y3C0 HS

Family and domain databases

InterProiView protein in InterPro
IPR019309 WASHC3
PANTHERiPTHR13015 PTHR13015, 2 hits
PfamiView protein in Pfam
PF10152 CCDC53, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWASC3_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3C0
Secondary accession number(s): B2RC74
, Q53FF0, Q6IAI4, Q96QK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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