SF3B6

Functionⁱ

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA (PubMed:12234937). Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing (PubMed:16432215). Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively (PubMed:16432215).3 Publications

GO - Molecular functionⁱ

RNA binding
Source: UniProtKB
Inferred from high throughput direct assayⁱ
- 22658674
- 22681889

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

blastocyst formation Source: Ensembl
mRNA splicing, via spliceosome
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	RNA-binding
Biological process	mRNA processing, mRNA splicing

Enzyme and pathway databases

Reactomeⁱ	R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-72165 mRNA Splicing - Minor Pathway

Reactomeⁱ

R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Splicing factor 3B subunit 6 Alternative name(s): Pre-mRNA branch site protein p14 SF3b 14 kDa subunit Short name: SF3B14a Spliceosome-associated protein, 14-kDa Splicing factor 3b, subunit 6, 14kDa
Gene namesⁱ	Name:SF3B6 Synonyms:SAP14, SF3B14, SF3B14A ORF Names:CGI-110, HSPC175, HT006
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 2

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000115128.6
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:30096 SF3B6
MIMⁱ	607835 gene
neXtProtⁱ	NX_Q9Y3B4

Keywords - Cellular componentⁱ

Nucleus, Spliceosome

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	51639
Open Targets More...OpenTargetsⁱ	ENSG00000115128

Polymorphism and mutation databases

BioMutaⁱ	SF3B14
DMDMⁱ	12585536

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000081725	1 – 125	Splicing factor 3B subunit 6Add BLAST		125

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	29	N6-acetyllysine; alternateCombined sources	1
Cross-linkⁱ	29	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residueⁱ	41	N6-acetyllysineCombined sources	1

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDⁱ	Q9Y3B4
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9Y3B4
PaxDbⁱ	Q9Y3B4
PeptideAtlas More...PeptideAtlasⁱ	Q9Y3B4
PRIDEⁱ	Q9Y3B4
ProteomicsDBⁱ	86002
TopDownProteomicsⁱ	Q9Y3B4

PTM databases

iPTMnetⁱ	Q9Y3B4
PhosphoSitePlusⁱ	Q9Y3B4
SwissPalmⁱ	Q9Y3B4

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000115128 Expressed in 197 organ(s), highest expression level in metanephros
Genevisibleⁱ	Q9Y3B4 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA034829 HPA034830

HPAⁱ

HPA034829
HPA034830

Interactionⁱ

Subunit structureⁱ

Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42 (PubMed:12234937, PubMed:12738865, PubMed:16432215, PubMed:27720643, PubMed:28541300). Within the SF3B complex interacts directly with SF3B1 (PubMed:21062891, PubMed:16432215, Ref. 20). The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2 (PubMed:27720643). Component of the U11/U12 snRNPs that are part of the U12-type spliceosome (PubMed:15146077).8 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
SF3B1	O75533-1	3	EBI-1046261,EBI-15565798

With

Entry

#Exp.

IntAct

Notes

SF3B1

O75533-1

3

EBI-1046261,EBI-15565798

Protein-protein interaction databases

BioGridⁱ	119651, 78 interactors
CORUMⁱ	Q9Y3B4
Database of interacting proteins More...DIPⁱ	DIP-44132N
IntActⁱ	Q9Y3B4, 23 interactors
Molecular INTeraction database More...MINTⁱ	Q9Y3B4
STRINGⁱ	9606.ENSP00000233468

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	19 – 25	Combined sources	7
Helixⁱ	32 – 40	Combined sources	9
Beta strandⁱ	45 – 51	Combined sources	7
Turnⁱ	54 – 58	Combined sources	5
Beta strandⁱ	60 – 66	Combined sources	7
Helixⁱ	67 – 77	Combined sources	11
Beta strandⁱ	81 – 84	Combined sources	4
Beta strandⁱ	88 – 91	Combined sources	4
Helixⁱ	94 – 97	Combined sources	4
Turnⁱ	98 – 100	Combined sources	3
Helixⁱ	103 – 117	Combined sources	15

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9Y3B4
SMRⁱ	Q9Y3B4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9Y3B4

EvolutionaryTraceⁱ

Q9Y3B4

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	19 – 94	RRMPROSITE-ProRule annotationAdd BLAST		76

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	16 – 29	Interaction with pre-mRNA branch siteAdd BLAST		14

Sequence similaritiesⁱ

Belongs to the SF3B6 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0114 Eukaryota ENOG4111IHX LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000005908
HOGENOMⁱ	HOG000276232
HOVERGENⁱ	HBG039634
InParanoidⁱ	Q9Y3B4
KEGG Orthology (KO) More...KOⁱ	K12833
OMAⁱ	VNRVLFV
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0RTZ
PhylomeDBⁱ	Q9Y3B4

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd12241 RRM_SF3B14, 1 hit
Gene3Dⁱ	3.30.70.330, 1 hit
InterProⁱ	View protein in InterPro IPR012677 Nucleotide-bd_a/b_plait_sf IPR035979 RBD_domain_sf IPR000504 RRM_dom IPR034150 SF3B14_RRM
PANTHERⁱ	PTHR12785:SF7 PTHR12785:SF7, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00076 RRM_1, 1 hit
SMARTⁱ	View protein in SMART SM00360 RRM, 1 hit
SUPFAMⁱ	SSF54928 SSF54928, 1 hit
PROSITEⁱ	View protein in PROSITE PS50102 RRM, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9Y3B4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV 
        60         70         80         90        100
GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK 
       110        120 
MDTKKKEEQL KLLKEKYGIN TDPPK

Length:125

Mass (Da):14,585

Last modified:November 1, 1999 - v1

Checksum:ⁱA1439DACB78A3208

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF401310 mRNA Translation: AAK94041.1 AF151868 mRNA Translation: AAD34105.1 AF161523 mRNA Translation: AAF29138.1 AF184213 mRNA Translation: AAG09698.1 AK311908 mRNA Translation: BAG34849.1 AC008073 Genomic DNA Translation: AAY14664.1 CH471053 Genomic DNA Translation: EAX00767.1 BC015463 mRNA Translation: AAH15463.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS1707.1
NCBI Reference Sequences More...RefSeqⁱ	NP_057131.1, NM_016047.3
UniGeneⁱ	Hs.177861

Genome annotation databases

Ensemblⁱ	ENST00000233468; ENSP00000233468; ENSG00000115128
GeneIDⁱ	51639
KEGGⁱ	hsa:51639
UCSC genome browser More...UCSCⁱ	uc002rev.4 human

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9Y3B4	Splicing factor 3B subunit 6		MOUSE		125	UniRef100_Q9Y3B4
Uncharacterized protein		AOTNA		131
SF3B6 isoform 1		PANTR		125
Pre-mRNA branch site protein p14		MACMU		125
Pre-mRNA branch site protein p14		CALJA		125
+91

Protein	Similar proteins		Species	Score	Length	Source
Q9Y3B4	Splicing factor 3B subunit 6		MOUSE		125	UniRef90_Q9Y3B4
Uncharacterized protein		AOTNA		131
SF3B6 isoform 1		PANTR		125
Pre-mRNA branch site protein p14		MACMU		125
Pre-mRNA branch site protein p14		CALJA		125
+100

Protein	Similar proteins		Species	Score	Length	Source
Q9Y3B4	Splicing factor 3B subunit 6		MOUSE		125	UniRef50_Q9Y3B4
Splicing factor 3B subunit 6		DROME		121
Splicing factor 3B subunit 6-like protein		ARATH		124
Uncharacterized protein		AOTNA		131
SF3B6 isoform 1		PANTR		125
+455

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF401310 mRNA Translation: AAK94041.1 AF151868 mRNA Translation: AAD34105.1 AF161523 mRNA Translation: AAF29138.1 AF184213 mRNA Translation: AAG09698.1 AK311908 mRNA Translation: BAG34849.1 AC008073 Genomic DNA Translation: AAY14664.1 CH471053 Genomic DNA Translation: EAX00767.1 BC015463 mRNA Translation: AAH15463.1
CCDSⁱ	CCDS1707.1
RefSeqⁱ	NP_057131.1, NM_016047.3
UniGeneⁱ	Hs.177861

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2F9D	X-ray	2.50	A/B	1-125	[»]
	2F9J	X-ray	3.00	A/B	1-125	[»]
	2FHO	NMR	-	B	8-93	[»]
	3LQV	X-ray	2.38	A/B	11-125	[»]
	5Z56	electron microscopy	5.10	5	1-125	[»]
	5Z57	electron microscopy	6.50	5	1-125	[»]
	5Z58	electron microscopy	4.90	5	1-125	[»]
	6FF4	electron microscopy	16.00	z	1-125	[»]
ProteinModelPortalⁱ	Q9Y3B4
SMRⁱ	Q9Y3B4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	119651, 78 interactors
CORUMⁱ	Q9Y3B4
DIPⁱ	DIP-44132N
IntActⁱ	Q9Y3B4, 23 interactors
MINTⁱ	Q9Y3B4
STRINGⁱ	9606.ENSP00000233468

PTM databases

iPTMnetⁱ	Q9Y3B4
PhosphoSitePlusⁱ	Q9Y3B4
SwissPalmⁱ	Q9Y3B4

Polymorphism and mutation databases

BioMutaⁱ	SF3B14
DMDMⁱ	12585536

Proteomic databases

EPDⁱ	Q9Y3B4
MaxQBⁱ	Q9Y3B4
PaxDbⁱ	Q9Y3B4
PeptideAtlasⁱ	Q9Y3B4
PRIDEⁱ	Q9Y3B4
ProteomicsDBⁱ	86002
TopDownProteomicsⁱ	Q9Y3B4

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	51639
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000233468; ENSP00000233468; ENSG00000115128
GeneIDⁱ	51639
KEGGⁱ	hsa:51639
UCSCⁱ	uc002rev.4 human

Organism-specific databases

CTDⁱ	51639
DisGeNETⁱ	51639
EuPathDBⁱ	HostDB:ENSG00000115128.6
GeneCardsⁱ	SF3B6
HGNCⁱ	HGNC:30096 SF3B6
HPAⁱ	HPA034829 HPA034830
MIMⁱ	607835 gene
neXtProtⁱ	NX_Q9Y3B4
OpenTargetsⁱ	ENSG00000115128
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0114 Eukaryota ENOG4111IHX LUCA
GeneTreeⁱ	ENSGT00390000005908
HOGENOMⁱ	HOG000276232
HOVERGENⁱ	HBG039634
InParanoidⁱ	Q9Y3B4
KOⁱ	K12833
OMAⁱ	VNRVLFV
OrthoDBⁱ	EOG091G0RTZ
PhylomeDBⁱ	Q9Y3B4

Enzyme and pathway databases

Reactomeⁱ	R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-72165 mRNA Splicing - Minor Pathway

Reactomeⁱ

R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway

Miscellaneous databases

ChiTaRSⁱ	SF3B6 human
EvolutionaryTraceⁱ	Q9Y3B4
GeneWikiⁱ	SF3B14
GenomeRNAiⁱ	51639
Protein Ontology More...PROⁱ	PR:Q9Y3B4
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000115128 Expressed in 197 organ(s), highest expression level in metanephros
Genevisibleⁱ	Q9Y3B4 HS

Family and domain databases

CDDⁱ	cd12241 RRM_SF3B14, 1 hit
Gene3Dⁱ	3.30.70.330, 1 hit
InterProⁱ	View protein in InterPro IPR012677 Nucleotide-bd_a/b_plait_sf IPR035979 RBD_domain_sf IPR000504 RRM_dom IPR034150 SF3B14_RRM
PANTHERⁱ	PTHR12785:SF7 PTHR12785:SF7, 1 hit
Pfamⁱ	View protein in Pfam PF00076 RRM_1, 1 hit
SMARTⁱ	View protein in SMART SM00360 RRM, 1 hit
SUPFAMⁱ	SSF54928 SSF54928, 1 hit
PROSITEⁱ	View protein in PROSITE PS50102 RRM, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SF3B6_HUMAN
Accessionⁱ	Q9Y3B4Primary (citable) accession number: Q9Y3B4 Secondary accession number(s): Q53TM1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
	Last sequence update:	November 1, 1999
	Last modified:	November 7, 2018
	This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - Q9Y3B4 (SF3B6_HUMAN)

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Splicing factor 3B subunit 6

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ