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Protein

Nitric oxide synthase-interacting protein

Gene

NOSIP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).By similarity3 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-203754 NOSIP mediated eNOS trafficking

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase-interacting protein
Alternative name(s):
E3 ubiquitin-protein ligase NOSIP (EC:2.3.2.27)
RING-type E3 ubiquitin transferase NOSIPCurated
eNOS-interacting protein
Gene namesi
Name:NOSIP
ORF Names:CGI-25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000142546.13
HGNCiHGNC:17946 NOSIP
MIMi616759 gene
neXtProtiNX_Q9Y314

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi51070
OpenTargetsiENSG00000142546
PharmGKBiPA134989259

Polymorphism and mutation databases

DMDMi74735248

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002805851 – 301Nitric oxide synthase-interacting proteinAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineCombined sources1
Modified residuei107PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y314
MaxQBiQ9Y314
PaxDbiQ9Y314
PeptideAtlasiQ9Y314
PRIDEiQ9Y314
ProteomicsDBi85958

PTM databases

iPTMnetiQ9Y314
PhosphoSitePlusiQ9Y314

Expressioni

Tissue specificityi

Expressed in heart, brain and lung. Present in endothelial cells (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000142546 Expressed in 230 organ(s), highest expression level in testis
CleanExiHS_NOSIP
ExpressionAtlasiQ9Y314 baseline and differential
GenevisibleiQ9Y314 HS

Organism-specific databases

HPAiHPA043464
HPA062132

Interactioni

Subunit structurei

Interacts with NOS1 and NOS3 (PubMed:11149895, PubMed:15548660). Interacts with PP2A holoenzyme, containing PPP2CA, PPP2CB, PPP2R1A and PPP2R2A subunits (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi119261, 31 interactors
IntActiQ9Y314, 7 interactors
MINTiQ9Y314
STRINGi9606.ENSP00000375726

Structurei

3D structure databases

ProteinModelPortaliQ9Y314
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 75U-box-likeAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 101Nuclear localization signalAdd BLAST24

Domaini

The U-box-like region is a truncated U-box domain. It is unknown whether it is functional or not.

Sequence similaritiesi

Belongs to the NOSIP family.Curated

Phylogenomic databases

eggNOGiKOG3039 Eukaryota
ENOG410XQYR LUCA
GeneTreeiENSGT00390000015505
HOGENOMiHOG000007498
HOVERGENiHBG082046
InParanoidiQ9Y314
KOiK13125
PhylomeDBiQ9Y314
TreeFamiTF314268

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR016818 NOSIP
IPR031790 Znf-NOSIP
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR13063 PTHR13063, 1 hit
PfamiView protein in Pfam
PF15906 zf-NOSIP, 1 hit
PIRSFiPIRSF023577 ENOS_interacting, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q9Y314-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL
60 70 80 90 100
QPCHDPVVTP DGYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK
110 120 130 140 150
ELQRAASQDH VRGFLEKESA IVSRPLNPFT AKALSGTSPD DVQPGPSVGP
160 170 180 190 200
PSKDKDKVLP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTPV
210 220 230 240 250
HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC
260 270 280 290 300
VEKLIRKDMV DPVTGDKLTD RDIIVLQRGG TGFAGSGVKL QAEKSRPVMQ

A
Length:301
Mass (Da):33,172
Last modified:November 1, 1999 - v1
Checksum:iFB77D6E31511C884
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A075B6F9A0A075B6F9_HUMAN
Nitric oxide synthase-interacting p...
NOSIP
304Annotation score:
M0R3B2M0R3B2_HUMAN
Nitric oxide synthase-interacting p...
NOSIP
241Annotation score:
M0R1K2M0R1K2_HUMAN
Nitric oxide synthase-interacting p...
NOSIP
176Annotation score:
M0QX85M0QX85_HUMAN
Nitric oxide synthase-interacting p...
NOSIP
96Annotation score:
A0A075B797A0A075B797_HUMAN
Nitric oxide synthase-interacting p...
NOSIP
232Annotation score:
M0R1T7M0R1T7_HUMAN
Nitric oxide synthase-interacting p...
NOSIP
63Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031169168T → M1 PublicationCorresponds to variant dbSNP:rs17850728Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132959 mRNA Translation: AAD27734.1
BC009299 mRNA Translation: AAH09299.1
BC010077 mRNA Translation: AAH10077.1
BC011249 mRNA Translation: AAH11249.1
CCDSiCCDS12772.1
RefSeqiNP_001257889.1, NM_001270960.1
NP_057037.1, NM_015953.4
XP_016882340.1, XM_017026851.1
XP_016882341.1, XM_017026852.1
UniGeneiHs.7236

Genome annotation databases

EnsembliENST00000596358; ENSP00000470034; ENSG00000142546
GeneIDi51070
KEGGihsa:51070
UCSCiuc002pol.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132959 mRNA Translation: AAD27734.1
BC009299 mRNA Translation: AAH09299.1
BC010077 mRNA Translation: AAH10077.1
BC011249 mRNA Translation: AAH11249.1
CCDSiCCDS12772.1
RefSeqiNP_001257889.1, NM_001270960.1
NP_057037.1, NM_015953.4
XP_016882340.1, XM_017026851.1
XP_016882341.1, XM_017026852.1
UniGeneiHs.7236

3D structure databases

ProteinModelPortaliQ9Y314
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119261, 31 interactors
IntActiQ9Y314, 7 interactors
MINTiQ9Y314
STRINGi9606.ENSP00000375726

PTM databases

iPTMnetiQ9Y314
PhosphoSitePlusiQ9Y314

Polymorphism and mutation databases

DMDMi74735248

Proteomic databases

EPDiQ9Y314
MaxQBiQ9Y314
PaxDbiQ9Y314
PeptideAtlasiQ9Y314
PRIDEiQ9Y314
ProteomicsDBi85958

Protocols and materials databases

DNASUi51070
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000596358; ENSP00000470034; ENSG00000142546
GeneIDi51070
KEGGihsa:51070
UCSCiuc002pol.5 human

Organism-specific databases

CTDi51070
DisGeNETi51070
EuPathDBiHostDB:ENSG00000142546.13
GeneCardsiNOSIP
HGNCiHGNC:17946 NOSIP
HPAiHPA043464
HPA062132
MIMi616759 gene
neXtProtiNX_Q9Y314
OpenTargetsiENSG00000142546
PharmGKBiPA134989259
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3039 Eukaryota
ENOG410XQYR LUCA
GeneTreeiENSGT00390000015505
HOGENOMiHOG000007498
HOVERGENiHBG082046
InParanoidiQ9Y314
KOiK13125
PhylomeDBiQ9Y314
TreeFamiTF314268

Enzyme and pathway databases

ReactomeiR-HSA-203754 NOSIP mediated eNOS trafficking

Miscellaneous databases

GeneWikiiNOSIP
GenomeRNAii51070
PROiPR:Q9Y314
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000142546 Expressed in 230 organ(s), highest expression level in testis
CleanExiHS_NOSIP
ExpressionAtlasiQ9Y314 baseline and differential
GenevisibleiQ9Y314 HS

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR016818 NOSIP
IPR031790 Znf-NOSIP
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR13063 PTHR13063, 1 hit
PfamiView protein in Pfam
PF15906 zf-NOSIP, 1 hit
PIRSFiPIRSF023577 ENOS_interacting, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOSIP_HUMAN
AccessioniPrimary (citable) accession number: Q9Y314
Secondary accession number(s): Q96FD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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