UniProtKB - Q9Y2X9 (ZN281_HUMAN)
Protein
Zinc finger protein 281
Gene
ZNF281
Organism
Homo sapiens (Human)
Status
Functioni
Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.By similarity2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 261 – 283 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 289 – 311 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 317 – 339 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 345 – 367 | C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
- transcription regulatory region sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- embryonic body morphogenesis Source: UniProtKB
- negative regulation of gene expression Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription, DNA-templated Source: GO_Central
- stem cell differentiation Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Differentiation, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9Y2X9 |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein 281Alternative name(s): GC-box-binding zinc finger protein 1 Transcription factor ZBP-99 Zinc finger DNA-binding protein 99 |
Gene namesi | Name:ZNF281 Synonyms:GZP1, ZBP99 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000162702.7 |
HGNCi | HGNC:13075, ZNF281 |
MIMi | 618703, gene |
neXtProti | NX_Q9Y2X9 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nuclear chromatin Source: GO_Central
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 23528 |
OpenTargetsi | ENSG00000162702 |
PharmGKBi | PA37651 |
Miscellaneous databases
Pharosi | Q9Y2X9, Tbio |
Polymorphism and mutation databases
BioMutai | ZNF281 |
DMDMi | 13124664 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047505 | 1 – 895 | Zinc finger protein 281Add BLAST | 895 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Cross-linki | 2 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 101 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 128 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 213 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 219 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 225 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 232 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 242 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 259 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 301 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 325 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 373 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Modified residuei | 395 | PhosphoserineCombined sources | 1 | ||
Cross-linki | 409 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 416 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 460 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 477 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Modified residuei | 484 | PhosphoserineCombined sources | 1 | ||
Cross-linki | 493 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 498 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 539 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 599 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 617 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 622 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Modified residuei | 651 | PhosphoserineCombined sources | 1 | ||
Cross-linki | 661 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 670 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Modified residuei | 785 | PhosphoserineCombined sources | 1 | ||
Cross-linki | 787 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 792 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 795 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Modified residuei | 807 | PhosphoserineCombined sources | 1 | ||
Cross-linki | 818 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 840 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Modified residuei | 888 | PhosphothreonineCombined sources | 1 | ||
Isoform 2 (identifier: Q9Y2X9-2) | |||||
Cross-linki | 65 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9Y2X9 |
jPOSTi | Q9Y2X9 |
MassIVEi | Q9Y2X9 |
PaxDbi | Q9Y2X9 |
PeptideAtlasi | Q9Y2X9 |
PRIDEi | Q9Y2X9 |
ProteomicsDBi | 1027 85932 [Q9Y2X9-1] |
PTM databases
GlyConnecti | 2864, 1 O-Linked glycan (1 site) |
GlyGeni | Q9Y2X9, 4 sites, 1 O-linked glycan (4 sites) |
iPTMneti | Q9Y2X9 |
PhosphoSitePlusi | Q9Y2X9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000162702, Expressed in secondary oocyte and 241 other tissues |
Genevisiblei | Q9Y2X9, HS |
Organism-specific databases
HPAi | ENSG00000162702, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 117074, 80 interactors |
CORUMi | Q9Y2X9 |
ELMi | Q9Y2X9 |
IntActi | Q9Y2X9, 59 interactors |
MINTi | Q9Y2X9 |
STRINGi | 9606.ENSP00000294740 |
Miscellaneous databases
RNActi | Q9Y2X9, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 4 – 37 | Gly-richAdd BLAST | 34 | |
Compositional biasi | 90 – 96 | Poly-Pro | 7 | |
Compositional biasi | 141 – 148 | Poly-His | 8 |
Sequence similaritiesi
Belongs to the krueppel C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 261 – 283 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 289 – 311 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 317 – 339 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 345 – 367 | C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000161586 |
HOGENOMi | CLU_017625_0_0_1 |
InParanoidi | Q9Y2X9 |
OMAi | NNMGNLA |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q9Y2X9 |
TreeFami | TF331779 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 4 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9Y2X9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKIGSGFLSG GGGTGSSGGS GSGGGGSGGG GGGGSSGRRA EMEPTFPQGM
60 70 80 90 100
VMFNHRLPPV TSFTRPAGSA APPPQCVLSS STSAAPAAEP PPPPAPDMTF
110 120 130 140 150
KKEPAASAAA FPSQRTSWGF LQSLVSIKQE KPADPEEQQS HHHHHHHHYG
160 170 180 190 200
GLFAGAEERS PGLGGGEGGS HGVIQDLSIL HQHVQQQPAQ HHRDVLLSSS
210 220 230 240 250
SRTDDHHGTE EPKQDTNVKK AKRPKPESQG IKAKRKPSAS SKPSLVGDGE
260 270 280 290 300
GAILSPSQKP HICDHCSAAF RSSYHLRRHV LIHTGERPFQ CSQCSMGFIQ
310 320 330 340 350
KYLLQRHEKI HSREKPFGCD QCSMKFIQKY HMERHKRTHS GEKPYKCDTC
360 370 380 390 400
QQYFSRTDRL LKHRRTCGEV IVKGATSAEP GSSNHTNMGN LAVLSQGNTS
410 420 430 440 450
SSRRKTKSKS IAIENKEQKT GKTNESQISN NINMQSYSVE MPTVSSSGGI
460 470 480 490 500
IGTGIDELQK RVPKLIFKKG SRKNTDKNYL NFVSPLPDIV GQKSLSGKPS
510 520 530 540 550
GSLGIVSNNS VETIGLLQST SGKQGQISSN YDDAMQFSKK RRYLPTASSN
560 570 580 590 600
SAFSINVGHM VSQQSVIQSA GVSVLDNEAP LSLIDSSALN AEIKSCHDKS
610 620 630 640 650
GIPDEVLQSI LDQYSNKSES QKEDPFNIAE PRVDLHTSGE HSELVQEENL
660 670 680 690 700
SPGTQTPSND KASMLQEYSK YLQQAFEKST NASFTLGHGF QFVSLSSPLH
710 720 730 740 750
NHTLFPEKQI YTTSPLECGF GQSVTSVLPS SLPKPPFGML FGSQPGLYLS
760 770 780 790 800
ALDATHQQLT PSQELDDLID SQKNLETSSA FQSSSQKLTS QKEQKNLESS
810 820 830 840 850
TGFQIPSQEL ASQIDPQKDI EPRTTYQIEN FAQAFGSQFK SGSRVPMTFI
860 870 880 890
TNSNGEVDHR VRTSVSDFSG YTNMMSDVSE PCSTRVKTPT SQSYR
Sequence cautioni
The sequence CAB70967 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 63 | F → L in BAG51134 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 206 | H → Y in AAH51905 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 482 | F → L in BAG51134 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 635 | L → I in BAG51134 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035576 | 527 | I → T in a breast cancer sample; somatic mutation. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054815 | 49 – 84 | Missing in isoform 2. CuratedAdd BLAST | 36 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF125158 mRNA Translation: AAD21084.1 AJ132591 mRNA Translation: CAB70967.1 Different initiation. AJ132592 mRNA Translation: CAB70968.1 AK022916 mRNA Translation: BAG51134.1 AC104461 Genomic DNA No translation available. BC051905 mRNA Translation: AAH51905.1 BC060820 mRNA Translation: AAH60820.1 |
CCDSi | CCDS1402.1 [Q9Y2X9-1] CCDS60384.1 [Q9Y2X9-2] |
PIRi | JC7089 |
RefSeqi | NP_001268222.1, NM_001281293.1 [Q9Y2X9-1] NP_001268223.1, NM_001281294.1 [Q9Y2X9-2] NP_036614.1, NM_012482.4 [Q9Y2X9-1] XP_016856376.1, XM_017000887.1 XP_016856377.1, XM_017000888.1 |
Genome annotation databases
Ensembli | ENST00000294740; ENSP00000294740; ENSG00000162702 [Q9Y2X9-1] ENST00000367352; ENSP00000356321; ENSG00000162702 [Q9Y2X9-2] ENST00000367353; ENSP00000356322; ENSG00000162702 [Q9Y2X9-1] |
GeneIDi | 23528 |
KEGGi | hsa:23528 |
UCSCi | uc001gve.5, human [Q9Y2X9-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF125158 mRNA Translation: AAD21084.1 AJ132591 mRNA Translation: CAB70967.1 Different initiation. AJ132592 mRNA Translation: CAB70968.1 AK022916 mRNA Translation: BAG51134.1 AC104461 Genomic DNA No translation available. BC051905 mRNA Translation: AAH51905.1 BC060820 mRNA Translation: AAH60820.1 |
CCDSi | CCDS1402.1 [Q9Y2X9-1] CCDS60384.1 [Q9Y2X9-2] |
PIRi | JC7089 |
RefSeqi | NP_001268222.1, NM_001281293.1 [Q9Y2X9-1] NP_001268223.1, NM_001281294.1 [Q9Y2X9-2] NP_036614.1, NM_012482.4 [Q9Y2X9-1] XP_016856376.1, XM_017000887.1 XP_016856377.1, XM_017000888.1 |
3D structure databases
SMRi | Q9Y2X9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 117074, 80 interactors |
CORUMi | Q9Y2X9 |
ELMi | Q9Y2X9 |
IntActi | Q9Y2X9, 59 interactors |
MINTi | Q9Y2X9 |
STRINGi | 9606.ENSP00000294740 |
PTM databases
GlyConnecti | 2864, 1 O-Linked glycan (1 site) |
GlyGeni | Q9Y2X9, 4 sites, 1 O-linked glycan (4 sites) |
iPTMneti | Q9Y2X9 |
PhosphoSitePlusi | Q9Y2X9 |
Polymorphism and mutation databases
BioMutai | ZNF281 |
DMDMi | 13124664 |
Proteomic databases
EPDi | Q9Y2X9 |
jPOSTi | Q9Y2X9 |
MassIVEi | Q9Y2X9 |
PaxDbi | Q9Y2X9 |
PeptideAtlasi | Q9Y2X9 |
PRIDEi | Q9Y2X9 |
ProteomicsDBi | 1027 85932 [Q9Y2X9-1] |
Protocols and materials databases
Antibodypediai | 20632, 193 antibodies |
Genome annotation databases
Ensembli | ENST00000294740; ENSP00000294740; ENSG00000162702 [Q9Y2X9-1] ENST00000367352; ENSP00000356321; ENSG00000162702 [Q9Y2X9-2] ENST00000367353; ENSP00000356322; ENSG00000162702 [Q9Y2X9-1] |
GeneIDi | 23528 |
KEGGi | hsa:23528 |
UCSCi | uc001gve.5, human [Q9Y2X9-1] |
Organism-specific databases
CTDi | 23528 |
DisGeNETi | 23528 |
EuPathDBi | HostDB:ENSG00000162702.7 |
GeneCardsi | ZNF281 |
HGNCi | HGNC:13075, ZNF281 |
HPAi | ENSG00000162702, Low tissue specificity |
MIMi | 618703, gene |
neXtProti | NX_Q9Y2X9 |
OpenTargetsi | ENSG00000162702 |
PharmGKBi | PA37651 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000161586 |
HOGENOMi | CLU_017625_0_0_1 |
InParanoidi | Q9Y2X9 |
OMAi | NNMGNLA |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q9Y2X9 |
TreeFami | TF331779 |
Enzyme and pathway databases
PathwayCommonsi | Q9Y2X9 |
Miscellaneous databases
BioGRID-ORCSi | 23528, 18 hits in 863 CRISPR screens |
ChiTaRSi | ZNF281, human |
GeneWikii | ZNF281 |
GenomeRNAii | 23528 |
Pharosi | Q9Y2X9, Tbio |
PROi | PR:Q9Y2X9 |
RNActi | Q9Y2X9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000162702, Expressed in secondary oocyte and 241 other tissues |
Genevisiblei | Q9Y2X9, HS |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 4 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZN281_HUMAN | |
Accessioni | Q9Y2X9Primary (citable) accession number: Q9Y2X9 Secondary accession number(s): A6NF48 Q9NY92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations