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Protein

ARF GTPase-activating protein GIT1

Gene

GIT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 34C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

  • ephrin receptor signaling pathway Source: Reactome
  • regulation of cytokinesis Source: UniProtKB
  • regulation of G-protein coupled receptor protein signaling pathway Source: ProtInc

Keywordsi

Molecular functionGTPase activation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-3928664 Ephrin signaling
SignaLinkiQ9Y2X7
SIGNORiQ9Y2X7

Names & Taxonomyi

Protein namesi
Recommended name:
ARF GTPase-activating protein GIT1
Short name:
ARF GAP GIT1
Alternative name(s):
Cool-associated and tyrosine-phosphorylated protein 1
Short name:
CAT-1
Short name:
CAT1
G protein-coupled receptor kinase-interactor 1
GRK-interacting protein 1
Gene namesi
Name:GIT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000108262.15
HGNCiHGNC:4272 GIT1
MIMi608434 gene
neXtProtiNX_Q9Y2X7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi28964
OpenTargetsiENSG00000108262
PharmGKBiPA28683

Polymorphism and mutation databases

BioMutaiGIT1
DMDMi45645212

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000742001 – 761ARF GTPase-activating protein GIT1Add BLAST761

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphotyrosineBy similarity1
Modified residuei359PhosphoserineBy similarity1
Modified residuei362PhosphoserineCombined sources1
Modified residuei364PhosphothreonineCombined sources1
Modified residuei370PhosphoserineCombined sources1
Modified residuei375PhosphoserineCombined sources1
Modified residuei383PhosphotyrosineCombined sources1
Modified residuei385PhosphoserineCombined sources1
Modified residuei388PhosphoserineCombined sources1
Modified residuei392PhosphothreonineCombined sources1
Modified residuei410PhosphoserineCombined sources1
Modified residuei413PhosphoserineBy similarity1
Modified residuei417PhosphoserineBy similarity1
Modified residuei480PhosphothreonineCombined sources1
Modified residuei498PhosphoserineCombined sources1
Modified residuei536PhosphoserineBy similarity1
Modified residuei537PhosphothreonineBy similarity1
Modified residuei545PhosphotyrosineCombined sources1
Modified residuei554PhosphotyrosineCombined sources1
Modified residuei561PhosphoserineBy similarity1
Modified residuei571PhosphoserineBy similarity1
Modified residuei592PhosphoserineCombined sources1
Modified residuei596PhosphoserineCombined sources1
Modified residuei601PhosphothreonineCombined sources1
Modified residuei630PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y2X7
PaxDbiQ9Y2X7
PeptideAtlasiQ9Y2X7
PRIDEiQ9Y2X7
ProteomicsDBi85928
85929 [Q9Y2X7-2]
85930 [Q9Y2X7-3]

PTM databases

iPTMnetiQ9Y2X7
PhosphoSitePlusiQ9Y2X7
SwissPalmiQ9Y2X7

Miscellaneous databases

PMAP-CutDBiQ9Y2X7

Expressioni

Gene expression databases

BgeeiENSG00000108262
CleanExiHS_GIT1
ExpressionAtlasiQ9Y2X7 baseline and differential
GenevisibleiQ9Y2X7 HS

Organism-specific databases

HPAiHPA004059
HPA004186

Interactioni

Subunit structurei

Interacts with G protein-coupled receptor kinases: GRK2, PPFIA1 and PPFIA4. Interacts with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1 (By similarity). Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with TGFB1I1. Interacts with SCRIB. Interacts with SDCCAG3 (PubMed:23108400).By similarity4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi118789, 79 interactors
CORUMiQ9Y2X7
IntActiQ9Y2X7, 43 interactors
MINTiQ9Y2X7
STRINGi9606.ENSP00000378338

Structurei

3D structure databases

ProteinModelPortaliQ9Y2X7
SMRiQ9Y2X7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 124Arf-GAPPROSITE-ProRule annotationAdd BLAST124
Repeati132 – 161ANK 1Add BLAST30
Repeati166 – 195ANK 2Add BLAST30
Repeati199 – 228ANK 3Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni251 – 415PTK2/FAK1-bindingBy similarityAdd BLAST165
Regioni254 – 367ARHGEF6-bindingBy similarityAdd BLAST114
Regioni637 – 761Interaction with PXN and TGFB1I1By similarityAdd BLAST125

Domaini

The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 34C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0818 Eukaryota
ENOG410XR8U LUCA
GeneTreeiENSGT00920000149085
HOGENOMiHOG000232135
HOVERGENiHBG012506
InParanoidiQ9Y2X7
KOiK05737
OMAiNNRARSM
OrthoDBiEOG091G03VD
PhylomeDBiQ9Y2X7
TreeFamiTF317762

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
IPR032352 GIT1/2_CC
IPR022018 GIT1_C
IPR013724 GIT_SHD
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF01412 ArfGap, 1 hit
PF12205 GIT1_C, 1 hit
PF16559 GIT_CC, 1 hit
PF08518 GIT_SHD, 2 hits
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SM00105 ArfGap, 1 hit
SM00555 GIT, 2 hits
SUPFAMiSSF48403 SSF48403, 2 hits
SSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
PS50115 ARFGAP, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2X7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRKGPRAEV CADCSAPDPG WASISRGVLV CDECCSVHRS LGRHISIVKH
60 70 80 90 100
LRHSAWPPTL LQMVHTLASN GANSIWEHSL LDPAQVQSGR RKANPQDKVH
110 120 130 140 150
PIKSEFIRAK YQMLAFVHKL PCRDDDGVTA KDLSKQLHSS VRTGNLETCL
160 170 180 190 200
RLLSLGAQAN FFHPEKGTTP LHVAAKAGQT LQAELLVVYG ADPGSPDVNG
210 220 230 240 250
RTPIDYARQA GHHELAERLV ECQYELTDRL AFYLCGRKPD HKNGHYIIPQ
260 270 280 290 300
MADSLDLSEL AKAAKKKLQA LSNRLFEELA MDVYDEVDRR ENDAVWLATQ
310 320 330 340 350
NHSTLVTERS AVPFLPVNPE YSATRNQGRQ KLARFNAREF ATLIIDILSE
360 370 380 390 400
AKRRQQGKSL SSPTDNLELS LRSQSDLDDQ HDYDSVASDE DTDQEPLRST
410 420 430 440 450
GATRSNRARS MDSSDLSDGA VTLQEYLELK KALATSEAKV QQLMKVNSSL
460 470 480 490 500
SDELRRLQRE IHKLQAENLQ LRQPPGPVPT PPLPSERAEH TPMAPGGSTH
510 520 530 540 550
RRDRQAFSMY EPGSALKPFG GPPGDELTTR LQPFHSTELE DDAIYSVHVP
560 570 580 590 600
AGLYRIRKGV SASAVPFTPS SPLLSCSQEG SRHTSKLSRH GSGADSDYEN
610 620 630 640 650
TQSGDPLLGL EGKRFLELGK EEDFHPELES LDGDLDPGLP STEDVILKTE
660 670 680 690 700
QVTKNIQELL RAAQEFKHDS FVPCSEKIHL AVTEMASLFP KRPALEPVRS
710 720 730 740 750
SLRLLNASAY RLQSECRKTV PPEPGAPVDF QLLTQQVIQC AYDIAKAAKQ
760
LVTITTREKK Q
Length:761
Mass (Da):84,341
Last modified:March 15, 2004 - v2
Checksum:i702744942A796399
GO
Isoform 2 (identifier: Q9Y2X7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     134-176: SKQLHSSVRT...GTTPLHVAAK → TGHRSWATPD...VPWCPPLSSP
     177-761: Missing.

Note: No experimental confirmation available.
Show »
Length:176
Mass (Da):19,160
Checksum:i2B5BB45555AB5201
GO
Isoform 3 (identifier: Q9Y2X7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     253-253: D → DRSRQKCMSQ

Show »
Length:770
Mass (Da):85,446
Checksum:i278AD8D706C32EE0
GO

Sequence cautioni

The sequence AAH48196 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121P → S in AAD28046 (PubMed:10896954).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009666134 – 176SKQLH…HVAAK → TGHRSWATPDINPHPNRATG TSALSAATLPGSQVPWCPPL SSP in isoform 2. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_009667177 – 761Missing in isoform 2. 1 PublicationAdd BLAST585
Alternative sequenceiVSP_040984253D → DRSRQKCMSQ in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124490 mRNA Translation: AAD28046.1
AK294785 mRNA Translation: BAG57910.1
AK299932 mRNA Translation: BAG61765.1
AC104564 Genomic DNA No translation available.
BC006227 mRNA Translation: AAH06227.2
BC048196 mRNA Translation: AAH48196.1 Different initiation.
CCDSiCCDS11250.1 [Q9Y2X7-1]
CCDS42290.1 [Q9Y2X7-3]
RefSeqiNP_001078923.1, NM_001085454.1 [Q9Y2X7-3]
NP_054749.2, NM_014030.3 [Q9Y2X7-1]
UniGeneiHs.514051

Genome annotation databases

EnsembliENST00000225394; ENSP00000225394; ENSG00000108262 [Q9Y2X7-1]
ENST00000394869; ENSP00000378338; ENSG00000108262 [Q9Y2X7-3]
GeneIDi28964
KEGGihsa:28964
UCSCiuc002hef.3 human [Q9Y2X7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGIT1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2X7
Secondary accession number(s): B4DGU9
, B4DSV3, Q86SS0, Q9BRJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: March 15, 2004
Last modified: July 18, 2018
This is version 189 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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