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Protein

Mediator of RNA polymerase II transcription subunit 16

Gene

MED16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

Caution

It is uncertain whether Met-1 or Met-13 is the initiator.Curated

GO - Molecular functioni

  • catalytic activity Source: InterPro
  • signaling receptor activity Source: UniProtKB
  • thyroid hormone receptor binding Source: UniProtKB
  • thyroid hormone receptor coactivator activity Source: UniProtKB
  • transcription coactivator activity Source: ProtInc
  • transcription coregulator activity Source: UniProtKB
  • vitamin D receptor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
SignaLinkiQ9Y2X0

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 16
Alternative name(s):
Mediator complex subunit 16
Thyroid hormone receptor-associated protein 5
Thyroid hormone receptor-associated protein complex 95 kDa component
Short name:
Trap95
Vitamin D3 receptor-interacting protein complex 92 kDa component
Short name:
DRIP92
Gene namesi
Name:MED16
Synonyms:DRIP92Imported, THRAP5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000175221.14
HGNCiHGNC:17556 MED16
MIMi604062 gene
neXtProtiNX_Q9Y2X0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10025
OpenTargetsiENSG00000175221
PharmGKBiPA162395406

Polymorphism and mutation databases

BioMutaiMED16
DMDMi62511180

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512921 – 877Mediator of RNA polymerase II transcription subunit 16Add BLAST877

Proteomic databases

EPDiQ9Y2X0
PaxDbiQ9Y2X0
PeptideAtlasiQ9Y2X0
PRIDEiQ9Y2X0
ProteomicsDBi85922
85923 [Q9Y2X0-2]
85924 [Q9Y2X0-3]
85925 [Q9Y2X0-4]
85926 [Q9Y2X0-5]

PTM databases

iPTMnetiQ9Y2X0
PhosphoSitePlusiQ9Y2X0

Expressioni

Gene expression databases

BgeeiENSG00000175221 Expressed in 217 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_MED16
ExpressionAtlasiQ9Y2X0 baseline and differential
GenevisibleiQ9Y2X0 HS

Organism-specific databases

HPAiHPA030685
HPA046495

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115342, 69 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiQ9Y2X0
DIPiDIP-31463N
IntActiQ9Y2X0, 29 interactors
MINTiQ9Y2X0
STRINGi9606.ENSP00000325612

Structurei

3D structure databases

ProteinModelPortaliQ9Y2X0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati21 – 71WD 1Add BLAST51
Repeati72 – 119WD 2Add BLAST48
Repeati120 – 165WD 3Add BLAST46
Repeati166 – 203WD 4Add BLAST38
Repeati204 – 257WD 5Add BLAST54
Repeati258 – 334WD 6Add BLAST77
Repeati335 – 415WD 7Add BLAST81
Repeati416 – 460WD 8Add BLAST45
Repeati461 – 495WD 9Add BLAST35

Sequence similaritiesi

Belongs to the Mediator complex subunit 16 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IDXV Eukaryota
ENOG410ZRDM LUCA
GeneTreeiENSGT00390000003821
HOGENOMiHOG000113526
HOVERGENiHBG054317
InParanoidiQ9Y2X0
KOiK15159
OMAiRPHVLNT
OrthoDBiEOG091G037H
PhylomeDBiQ9Y2X0

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR021665 Mediator_Med16
IPR011041 Quinoprot_gluc/sorb_DH
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR13224 PTHR13224, 1 hit
PfamiView protein in Pfam
PF11635 Med16, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 1 hit
SUPFAMiSSF50952 SSF50952, 1 hit
SSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 19 potential isoforms that are computationally mapped.Show allAlign All

Isoform 11 Publication (identifier: Q9Y2X0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCDLRRPAAG GMMDLAYVCE WEKWSKSTHC PSVPLACAWS CRNLIAFTMD
60 70 80 90 100
LRSDDQDLTR MIHILDTEHP WDLHSIPSEH HEAITCLEWD QSGSRLLSAD
110 120 130 140 150
ADGQIKCWSM ADHLANSWES SVGSLVEGDP IVALSWLHNG VKLALHVEKS
160 170 180 190 200
GASSFGEKFS RVKFSPSLTL FGGKPMEGWI AVTVSGLVTV SLLKPSGQVL
210 220 230 240 250
TSTESLCRLR GRVALADIAF TGGGNIVVAT ADGSSASPVQ FYKVCVSVVS
260 270 280 290 300
EKCRIDTEIL PSLFMRCTTD LNRKDKFPAI THLKFLARDM SEQVLLCASS
310 320 330 340 350
QTSSIVECWS LRKEGLPVNN IFQQISPVVG DKQPTILKWR ILSATNDLDR
360 370 380 390 400
VSAVALPKLP ISLTNTDLKV ASDTQFYPGL GLALAFHDGS VHIVHRLSLQ
410 420 430 440 450
TMAVFYSSAA PRPVDEPAMK RPRTAGPAVH LKAMQLSWTS LALVGIDSHG
460 470 480 490 500
KLSVLRLSPS MGHPLEVGLA LRHLLFLLEY CMVTGYDWWD ILLHVQPSMV
510 520 530 540 550
QSLVEKLHEE YTRQTAALQQ VLSTRILAMK ASLCKLSPCT VTRVCDYHTK
560 570 580 590 600
LFLIAISSTL KSLLRPHFLN TPDKSPGDRL TEICTKITDV DIDKVMINLK
610 620 630 640 650
TEEFVLDMNT LQALQQLLQW VGDFVLYLLA SLPNQGSLLR PGHSFLRDGT
660 670 680 690 700
SLGMLRELMV VIRIWGLLKP SCLPVYTATS DTQDSMSLLF RLLTKLWICC
710 720 730 740 750
RDEGPASEPD EALVDECCLL PSQLLIPSLD WLPASDGLVS RLQPKQPLRL
760 770 780 790 800
QFGRAPTLPG SAATLQLDGL ARAPGQPKID HLRRLHLGAC PTEECKACTR
810 820 830 840 850
CGCVTMLKSP NRTTAVKQWE QRWIKNCLAV EGRGPDACVT SRASEEAPAF
860 870
VQLGPQSTHH SPRTPRSLDH LHPEDRP
Length:877
Mass (Da):96,793
Last modified:April 12, 2005 - v2
Checksum:i15173F237A75D230
GO
Isoform 2Curated (identifier: Q9Y2X0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     829-877: AVEGRGPDACVTSRASEEAPAFVQLGPQSTHHSPRTPRSLDHLHPEDRP → CGGLWWRVPLSYP

Show »
Length:841
Mass (Da):93,029
Checksum:i2D9CAF68001320C3
GO
Isoform 3Curated (identifier: Q9Y2X0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     700-700: C → FPSTGPCSVWVLLGWQPLPG
     829-877: AVEGRGPDACVTSRASEEAPAFVQLGPQSTHHSPRTPRSLDHLHPEDRP → CGGLWWRVPLSYP

Note: No experimental confirmation available.Curated
Show »
Length:860
Mass (Da):95,050
Checksum:i546EA25BE3AB56CF
GO
Isoform 4Curated (identifier: Q9Y2X0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     636-751: GSLLRPGHSF...LQPKQPLRLQ → VAMRAQRASR...CSSRPTEPRR
     752-877: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:751
Mass (Da):82,179
Checksum:i6B77C11B864902BD
GO
Isoform 5 (identifier: Q9Y2X0-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     636-663: GSLLRPGHSFLRDGTSLGMLRELMVVIR → PCPTSEPCPTSEPSPTSEPSPTSEPSSP
     664-877: Missing.

Note: No experimental confirmation available.
Show »
Length:663
Mass (Da):72,967
Checksum:i311F7B98C3912283
GO

Computationally mapped potential isoform sequencesi

There are 19 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B9TWZ6B9TWZ6_HUMAN
Mediator of RNA polymerase II trans...
MED16
537Annotation score:
U3KQ43U3KQ43_HUMAN
Mediator of RNA polymerase II trans...
MED16
663Annotation score:
B9TX11B9TX11_HUMAN
Mediator of RNA polymerase II trans...
MED16
219Annotation score:
B9TX12B9TX12_HUMAN
Mediator of RNA polymerase II trans...
MED16
218Annotation score:
K7EKS6K7EKS6_HUMAN
Mediator of RNA polymerase II trans...
MED16
462Annotation score:
U3KQB4U3KQB4_HUMAN
Mediator of RNA polymerase II trans...
MED16
505Annotation score:
A0A087WU12A0A087WU12_HUMAN
Mediator of RNA polymerase II trans...
MED16
79Annotation score:
A0A0J9YXL6A0A0J9YXL6_HUMAN
Mediator of RNA polymerase II trans...
MED16
283Annotation score:
A0A0J9YW49A0A0J9YW49_HUMAN
Mediator of RNA polymerase II trans...
MED16
371Annotation score:
A0A0J9YXA0A0A0J9YXA0_HUMAN
Mediator of RNA polymerase II trans...
MED16
497Annotation score:
There are more potential isoformsShow all

Sequence cautioni

The sequence AAD31087 differs from that shown. Reason: Frameshift at position 609.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46A → T in AAD30032 (PubMed:10198638).Curated1
Sequence conflicti211G → A in AAD31087 (PubMed:10235266).Curated1
Sequence conflicti412R → K in AAD30032 (PubMed:10198638).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053958770L → F. Corresponds to variant dbSNP:rs34859566Ensembl.1
Natural variantiVAR_053959874E → K. Corresponds to variant dbSNP:rs13090Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_051721636 – 751GSLLR…PLRLQ → VAMRAQRASRTRRWWMNAAC CPASCLSPAWTGCQPATAWL AACSPSSPFVCSLAGRPRCL AVLPPCSSTASPGPQASPRS TTCGGCTLALAPRRNARPAP GAAVSPCSSRPTEPRR in isoform 4. 1 PublicationAdd BLAST116
Alternative sequenceiVSP_028749636 – 663GSLLR…MVVIR → PCPTSEPCPTSEPSPTSEPS PTSEPSSP in isoform 5. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_028750664 – 877Missing in isoform 5. 1 PublicationAdd BLAST214
Alternative sequenceiVSP_051722700C → FPSTGPCSVWVLLGWQPLPG in isoform 3. 1 Publication1
Alternative sequenceiVSP_051723752 – 877Missing in isoform 4. 1 PublicationAdd BLAST126
Alternative sequenceiVSP_051724829 – 877AVEGR…PEDRP → CGGLWWRVPLSYP in isoform 2 and isoform 3. 2 PublicationsAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF121228 mRNA Translation: AAD30032.1
AF106934 mRNA Translation: AAD31087.1 Frameshift.
BC004554 mRNA Translation: AAH04554.1
BC007853 mRNA Translation: AAH07853.2
BC011841 mRNA Translation: AAH11841.1
BC017282 mRNA Translation: AAH17282.1
CCDSiCCDS12047.1 [Q9Y2X0-1]
RefSeqiNP_005472.2, NM_005481.2 [Q9Y2X0-1]
UniGeneiHs.365207

Genome annotation databases

EnsembliENST00000312090; ENSP00000308528; ENSG00000175221 [Q9Y2X0-3]
ENST00000325464; ENSP00000325612; ENSG00000175221 [Q9Y2X0-1]
ENST00000395808; ENSP00000379153; ENSG00000175221 [Q9Y2X0-2]
ENST00000589119; ENSP00000464810; ENSG00000175221 [Q9Y2X0-1]
GeneIDi10025
KEGGihsa:10025
UCSCiuc002lqd.2 human [Q9Y2X0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF121228 mRNA Translation: AAD30032.1
AF106934 mRNA Translation: AAD31087.1 Frameshift.
BC004554 mRNA Translation: AAH04554.1
BC007853 mRNA Translation: AAH07853.2
BC011841 mRNA Translation: AAH11841.1
BC017282 mRNA Translation: AAH17282.1
CCDSiCCDS12047.1 [Q9Y2X0-1]
RefSeqiNP_005472.2, NM_005481.2 [Q9Y2X0-1]
UniGeneiHs.365207

3D structure databases

ProteinModelPortaliQ9Y2X0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115342, 69 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiQ9Y2X0
DIPiDIP-31463N
IntActiQ9Y2X0, 29 interactors
MINTiQ9Y2X0
STRINGi9606.ENSP00000325612

PTM databases

iPTMnetiQ9Y2X0
PhosphoSitePlusiQ9Y2X0

Polymorphism and mutation databases

BioMutaiMED16
DMDMi62511180

Proteomic databases

EPDiQ9Y2X0
PaxDbiQ9Y2X0
PeptideAtlasiQ9Y2X0
PRIDEiQ9Y2X0
ProteomicsDBi85922
85923 [Q9Y2X0-2]
85924 [Q9Y2X0-3]
85925 [Q9Y2X0-4]
85926 [Q9Y2X0-5]

Protocols and materials databases

DNASUi10025
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312090; ENSP00000308528; ENSG00000175221 [Q9Y2X0-3]
ENST00000325464; ENSP00000325612; ENSG00000175221 [Q9Y2X0-1]
ENST00000395808; ENSP00000379153; ENSG00000175221 [Q9Y2X0-2]
ENST00000589119; ENSP00000464810; ENSG00000175221 [Q9Y2X0-1]
GeneIDi10025
KEGGihsa:10025
UCSCiuc002lqd.2 human [Q9Y2X0-1]

Organism-specific databases

CTDi10025
DisGeNETi10025
EuPathDBiHostDB:ENSG00000175221.14
GeneCardsiMED16
H-InvDBiHIX0015548
HGNCiHGNC:17556 MED16
HPAiHPA030685
HPA046495
MIMi604062 gene
neXtProtiNX_Q9Y2X0
OpenTargetsiENSG00000175221
PharmGKBiPA162395406
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IDXV Eukaryota
ENOG410ZRDM LUCA
GeneTreeiENSGT00390000003821
HOGENOMiHOG000113526
HOVERGENiHBG054317
InParanoidiQ9Y2X0
KOiK15159
OMAiRPHVLNT
OrthoDBiEOG091G037H
PhylomeDBiQ9Y2X0

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
SignaLinkiQ9Y2X0

Miscellaneous databases

ChiTaRSiMED16 human
GeneWikiiMED16
GenomeRNAii10025
PROiPR:Q9Y2X0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000175221 Expressed in 217 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_MED16
ExpressionAtlasiQ9Y2X0 baseline and differential
GenevisibleiQ9Y2X0 HS

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR021665 Mediator_Med16
IPR011041 Quinoprot_gluc/sorb_DH
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR13224 PTHR13224, 1 hit
PfamiView protein in Pfam
PF11635 Med16, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 1 hit
SUPFAMiSSF50952 SSF50952, 1 hit
SSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMED16_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2X0
Secondary accession number(s): Q6PJT2
, Q96AD4, Q96I35, Q9Y652
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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