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Protein

Guanine deaminase

Gene

GDA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.By similarity

Catalytic activityi

Guanine + H2O = xanthine + NH3.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: guanine degradation

This protein is involved in step 1 of the subpathway that synthesizes xanthine from guanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Guanine deaminase (GDA)
This subpathway is part of the pathway guanine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes xanthine from guanine, the pathway guanine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi82Zinc; via tele nitrogenCombined sources1 Publication1
Metal bindingi84Zinc; via tele nitrogenCombined sources1 Publication1
Metal bindingi240Zinc; via tele nitrogenCombined sources1 Publication1
Metal bindingi330ZincCombined sources1 Publication1
Binding sitei330SubstrateCombined sources1 Publication1

GO - Molecular functioni

  • guanine deaminase activity Source: Reactome
  • zinc ion binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:HS04276-MONOMER
BRENDAi3.5.4.3 2681
ReactomeiR-HSA-74259 Purine catabolism
SABIO-RKiQ9Y2T3
UniPathwayi
UPA00603;UER00660

Protein family/group databases

MEROPSiM38.981

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine deaminase (EC:3.5.4.3)
Short name:
Guanase
Short name:
Guanine aminase
Alternative name(s):
Guanine aminohydrolase
Short name:
GAH
p51-nedasin
Gene namesi
Name:GDA
Synonyms:KIAA1258
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000119125.16
HGNCiHGNC:4212 GDA
MIMi139260 gene
neXtProtiNX_Q9Y2T3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi9615
OpenTargetsiENSG00000119125
PharmGKBiPA28625

Chemistry databases

ChEMBLiCHEMBL3129

Polymorphism and mutation databases

BioMutaiGDA
DMDMi9910736

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001222981 – 454Guanine deaminaseAdd BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei453PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y2T3
PaxDbiQ9Y2T3
PeptideAtlasiQ9Y2T3
PRIDEiQ9Y2T3
ProteomicsDBi85890
85891 [Q9Y2T3-2]
85892 [Q9Y2T3-3]

PTM databases

iPTMnetiQ9Y2T3
PhosphoSitePlusiQ9Y2T3

Expressioni

Gene expression databases

BgeeiENSG00000119125 Expressed in 137 organ(s), highest expression level in jejunal mucosa
CleanExiHS_GDA
ExpressionAtlasiQ9Y2T3 baseline and differential
GenevisibleiQ9Y2T3 HS

Organism-specific databases

HPAiHPA019352
HPA024099
HPA030387

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi114976, 42 interactors
MINTiQ9Y2T3
STRINGi9606.ENSP00000238018

Chemistry databases

BindingDBiQ9Y2T3

Structurei

Secondary structure

1454
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y2T3
SMRiQ9Y2T3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y2T3

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 87Substrate bindingCombined sources1 Publication4
Regioni213 – 214Substrate bindingCombined sources1 Publication2
Regioni240 – 243Substrate bindingCombined sources1 Publication4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3968 Eukaryota
COG0402 LUCA
GeneTreeiENSGT00390000017130
HOGENOMiHOG000257692
HOVERGENiHBG005930
InParanoidiQ9Y2T3
KOiK01487
OMAiASYFATN
OrthoDBiEOG091G07AM
PhylomeDBiQ9Y2T3
TreeFamiTF324539

Family and domain databases

CDDicd01303 GDEase, 1 hit
Gene3Di2.30.40.10, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR014311 Guanine_deaminase
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
PANTHERiPTHR11271:SF6 PTHR11271:SF6, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR02967 guan_deamin, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y2T3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCAAQMPPLA HIFRGTFVHS TWTCPMEVLR DHLLGVSDSG KIVFLEEASQ
60 70 80 90 100
QEKLAKEWCF KPCEIRELSH HEFFMPGLVD THIHASQYSF AGSSIDLPLL
110 120 130 140 150
EWLTKYTFPA EHRFQNIDFA EEVYTRVVRR TLKNGTTTAC YFATIHTDSS
160 170 180 190 200
LLLADITDKF GQRAFVGKVC MDLNDTFPEY KETTEESIKE TERFVSEMLQ
210 220 230 240 250
KNYSRVKPIV TPRFSLSCSE TLMGELGNIA KTRDLHIQSH ISENRDEVEA
260 270 280 290 300
VKNLYPSYKN YTSVYDKNNL LTNKTVMAHG CYLSAEELNV FHERGASIAH
310 320 330 340 350
CPNSNLSLSS GFLNVLEVLK HEVKIGLGTD VAGGYSYSML DAIRRAVMVS
360 370 380 390 400
NILLINKVNE KSLTLKEVFR LATLGGSQAL GLDGEIGNFE VGKEFDAILI
410 420 430 440 450
NPKASDSPID LFYGDFFGDI SEAVIQKFLY LGDDRNIEEV YVGGKQVVPF

SSSV
Length:454
Mass (Da):51,003
Last modified:November 1, 1999 - v1
Checksum:iA45C868E6EEA7380
GO
Isoform 2 (identifier: Q9Y2T3-2) [UniParc]FASTAAdd to basket
Also known as: c

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.

Show »
Length:380
Mass (Da):42,445
Checksum:i6AD0F99117A467CE
GO
Isoform 3 (identifier: Q9Y2T3-3) [UniParc]FASTAAdd to basket
Also known as: a

The sequence of this isoform differs from the canonical sequence as follows:
     454-454: V → VKETIHLPASSPHPPPFP

Show »
Length:471
Mass (Da):52,837
Checksum:i5B9BF9B0E1111F88
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5SZC3Q5SZC3_HUMAN
Guanine deaminase
GDA
179Annotation score:
Q5SZC5Q5SZC5_HUMAN
Guanine deaminase
GDA
195Annotation score:
Q5SZC6Q5SZC6_HUMAN
Guanine deaminase
GDA
393Annotation score:
H0YDZ7H0YDZ7_HUMAN
Guanine deaminase
GDA
176Annotation score:

Sequence cautioni

The sequence BAA86572 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti316 – 318LEV → ARI in AAG40469 (Ref. 3) Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0420751 – 74Missing in isoform 2. 2 PublicationsAdd BLAST74
Alternative sequenceiVSP_042076454V → VKETIHLPASSPHPPPFP in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095286 mRNA Translation: AAD25978.1
AF019638 mRNA Translation: AAF13301.1
AF144745 mRNA Translation: AAG40469.1
AB033084 mRNA Translation: BAA86572.1 Different initiation.
AK300418 mRNA Translation: BAG62147.1
AK315988 mRNA Translation: BAH14359.1
AL583829 Genomic DNA No translation available.
AL590311 Genomic DNA No translation available.
AL135924 Genomic DNA No translation available.
CH471089 Genomic DNA Translation: EAW62529.1
BC053584 mRNA Translation: AAH53584.1
CCDSiCCDS56576.1 [Q9Y2T3-3]
CCDS56577.1 [Q9Y2T3-2]
CCDS6641.1 [Q9Y2T3-1]
RefSeqiNP_001229434.1, NM_001242505.2 [Q9Y2T3-3]
NP_001229435.1, NM_001242506.2 [Q9Y2T3-2]
NP_001229436.1, NM_001242507.2 [Q9Y2T3-2]
NP_004284.1, NM_004293.4 [Q9Y2T3-1]
XP_005252374.1, XM_005252317.1 [Q9Y2T3-3]
XP_016870825.1, XM_017015336.1
UniGeneiHs.494163

Genome annotation databases

EnsembliENST00000238018; ENSP00000238018; ENSG00000119125 [Q9Y2T3-3]
ENST00000358399; ENSP00000351170; ENSG00000119125 [Q9Y2T3-1]
ENST00000475764; ENSP00000436619; ENSG00000119125 [Q9Y2T3-1]
ENST00000545168; ENSP00000437972; ENSG00000119125 [Q9Y2T3-2]
GeneIDi9615
KEGGihsa:9615
UCSCiuc004aiq.4 human [Q9Y2T3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095286 mRNA Translation: AAD25978.1
AF019638 mRNA Translation: AAF13301.1
AF144745 mRNA Translation: AAG40469.1
AB033084 mRNA Translation: BAA86572.1 Different initiation.
AK300418 mRNA Translation: BAG62147.1
AK315988 mRNA Translation: BAH14359.1
AL583829 Genomic DNA No translation available.
AL590311 Genomic DNA No translation available.
AL135924 Genomic DNA No translation available.
CH471089 Genomic DNA Translation: EAW62529.1
BC053584 mRNA Translation: AAH53584.1
CCDSiCCDS56576.1 [Q9Y2T3-3]
CCDS56577.1 [Q9Y2T3-2]
CCDS6641.1 [Q9Y2T3-1]
RefSeqiNP_001229434.1, NM_001242505.2 [Q9Y2T3-3]
NP_001229435.1, NM_001242506.2 [Q9Y2T3-2]
NP_001229436.1, NM_001242507.2 [Q9Y2T3-2]
NP_004284.1, NM_004293.4 [Q9Y2T3-1]
XP_005252374.1, XM_005252317.1 [Q9Y2T3-3]
XP_016870825.1, XM_017015336.1
UniGeneiHs.494163

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2UZ9X-ray2.30A1-454[»]
3E0LX-ray2.37A/B1-454[»]
4AQLX-ray1.99A1-454[»]
ProteinModelPortaliQ9Y2T3
SMRiQ9Y2T3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114976, 42 interactors
MINTiQ9Y2T3
STRINGi9606.ENSP00000238018

Chemistry databases

BindingDBiQ9Y2T3
ChEMBLiCHEMBL3129

Protein family/group databases

MEROPSiM38.981

PTM databases

iPTMnetiQ9Y2T3
PhosphoSitePlusiQ9Y2T3

Polymorphism and mutation databases

BioMutaiGDA
DMDMi9910736

Proteomic databases

EPDiQ9Y2T3
PaxDbiQ9Y2T3
PeptideAtlasiQ9Y2T3
PRIDEiQ9Y2T3
ProteomicsDBi85890
85891 [Q9Y2T3-2]
85892 [Q9Y2T3-3]

Protocols and materials databases

DNASUi9615
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000238018; ENSP00000238018; ENSG00000119125 [Q9Y2T3-3]
ENST00000358399; ENSP00000351170; ENSG00000119125 [Q9Y2T3-1]
ENST00000475764; ENSP00000436619; ENSG00000119125 [Q9Y2T3-1]
ENST00000545168; ENSP00000437972; ENSG00000119125 [Q9Y2T3-2]
GeneIDi9615
KEGGihsa:9615
UCSCiuc004aiq.4 human [Q9Y2T3-1]

Organism-specific databases

CTDi9615
DisGeNETi9615
EuPathDBiHostDB:ENSG00000119125.16
GeneCardsiGDA
HGNCiHGNC:4212 GDA
HPAiHPA019352
HPA024099
HPA030387
MIMi139260 gene
neXtProtiNX_Q9Y2T3
OpenTargetsiENSG00000119125
PharmGKBiPA28625
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3968 Eukaryota
COG0402 LUCA
GeneTreeiENSGT00390000017130
HOGENOMiHOG000257692
HOVERGENiHBG005930
InParanoidiQ9Y2T3
KOiK01487
OMAiASYFATN
OrthoDBiEOG091G07AM
PhylomeDBiQ9Y2T3
TreeFamiTF324539

Enzyme and pathway databases

UniPathwayi
UPA00603;UER00660

BioCyciMetaCyc:HS04276-MONOMER
BRENDAi3.5.4.3 2681
ReactomeiR-HSA-74259 Purine catabolism
SABIO-RKiQ9Y2T3

Miscellaneous databases

ChiTaRSiGDA human
EvolutionaryTraceiQ9Y2T3
GenomeRNAii9615
PROiPR:Q9Y2T3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000119125 Expressed in 137 organ(s), highest expression level in jejunal mucosa
CleanExiHS_GDA
ExpressionAtlasiQ9Y2T3 baseline and differential
GenevisibleiQ9Y2T3 HS

Family and domain databases

CDDicd01303 GDEase, 1 hit
Gene3Di2.30.40.10, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR014311 Guanine_deaminase
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
PANTHERiPTHR11271:SF6 PTHR11271:SF6, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR02967 guan_deamin, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGUAD_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2T3
Secondary accession number(s): B4DTY5
, Q5SZC7, Q9H335, Q9ULG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
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