UniProtKB - Q9Y2P5 (S27A5_HUMAN)
Protein
Bile acyl-CoA synthetase
Gene
SLC27A5
Organism
Homo sapiens (Human)
Status
Functioni
Acyl-CoA synthetase that catalyzes the activation of bile acids via formation of bile acid CoA thioesters which is necessary for their subsequent conjugation with glycine or taurine (PubMed:10749848, PubMed:11980911). Both primary bile acids (cholic acid and chenodeoxycholic acid) and secondary bile acids (deoxycholic acid and lithocholic acid) are the principal substrates (PubMed:10749848, PubMed:11980911). Also exhibits acyl CoA synthetase activity that activates very long-chain fatty acids (VLCFAs) by catalyzing the formation of fatty acyl-CoA (PubMed:10479480). In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol (PubMed:11980911). Exhibits long-chain fatty acids (LCFA) transport activity (PubMed:20530735). Plays an important role in hepatic fatty acid uptake and bile acid reconjugation and recycling but not in de novo synthesis of bile acids (By similarity).By similarity4 Publications
Catalytic activityi
- EC:6.2.1.73 PublicationsThis reaction proceeds in the forward3 Publications direction.
- (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oate + ATP + CoA = (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oyl-CoA + AMP + diphosphate1 PublicationEC:6.2.1.71 PublicationThis reaction proceeds in the forward1 Publication direction.
- a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the backward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Activity regulationi
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA) inhibits the activation of cholate.1 Publication
Kineticsi
- KM=2.8 µM for cholate1 Publication
- Vmax=3.8 nmol/min/mg enzyme with cholate as substrate for bile acyl-CoA synthetase activity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 292 – 303 | AMPSequence analysisAdd BLAST | 12 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cholate-CoA ligase activity Source: UniProtKB
- fatty acid transmembrane transporter activity Source: GO_Central
- long-chain fatty acid-CoA ligase activity Source: GO_Central
- long-chain fatty acid transporter activity Source: UniProtKB
- oxidoreductase activity Source: UniProtKB-KW
- protein-containing complex binding Source: Ensembl
- very long-chain fatty acid-CoA ligase activity Source: UniProtKB
GO - Biological processi
- bile acid and bile salt transport Source: Reactome
- bile acid biosynthetic process Source: UniProtKB
- bile acid metabolic process Source: GO_Central
- ketone body biosynthetic process Source: GO_Central
- long-chain fatty acid import across plasma membrane Source: GO_Central
- triglyceride mobilization Source: GO_Central
- very long-chain fatty acid metabolic process Source: UniProtKB
Keywordsi
Molecular function | Ligase, Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9Y2P5 |
Reactomei | R-HSA-159418, Recycling of bile acids and salts R-HSA-193368, Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-HSA-193775, Synthesis of bile acids and bile salts via 24-hydroxycholesterol |
Protein family/group databases
TCDBi | 4.C.1.1.13, the fatty acid transporter (fat) family |
Chemistry databases
SwissLipidsi | SLP:000000429 SLP:000001315 [Q9Y2P5-1] |
Names & Taxonomyi
Protein namesi | Recommended name: Bile acyl-CoA synthetase1 Publication (EC:6.2.1.73 Publications)Short name: BACS1 Publication Alternative name(s): Bile acid-CoA ligaseBy similarity Short name: BA-CoA ligaseBy similarity Short name: BALBy similarity Cholate--CoA ligase1 Publication Fatty acid transport protein 51 Publication Short name: FATP-51 Publication Fatty-acid-coenzyme A ligase, very long-chain 3 Solute carrier family 27 member 5 Very long-chain acyl-CoA synthetase homolog 22 Publications Short name: VLCS-H22 Publications Short name: VLCSH22 Publications Very long-chain acyl-CoA synthetase-related proteinBy similarity Short name: VLACS-relatedBy similarity Short name: VLACSRBy similarity |
Gene namesi | Name:SLC27A5 Synonyms:ACSB, ACSVL6, FACVL3, FATP5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000083807.9 |
HGNCi | HGNC:10999, SLC27A5 |
MIMi | 603314, gene |
neXtProti | NX_Q9Y2P5 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence analysis
- Microsome By similarity
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: Reactome
- integral component of endoplasmic reticulum membrane Source: UniProtKB
Plasma Membrane
- basal plasma membrane Source: Ensembl
Other locations
- protein-containing complex Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 30 | Cytoplasmic1 PublicationAdd BLAST | 30 | |
Transmembranei | 31 – 51 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 56 – 76 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 77 – 690 | Cytoplasmic1 PublicationAdd BLAST | 614 |
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 10998 |
MalaCardsi | SLC27A5 |
OpenTargetsi | ENSG00000083807 |
Orphaneti | 276066, Bile acid CoA ligase deficiency and defective amidation |
PharmGKBi | PA35873 |
Miscellaneous databases
Pharosi | Q9Y2P5, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5196 |
Polymorphism and mutation databases
BioMutai | SLC27A5 |
DMDMi | 74739456 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193213 | 1 – 690 | Bile acyl-CoA synthetaseAdd BLAST | 690 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 501 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q9Y2P5 |
MassIVEi | Q9Y2P5 |
PaxDbi | Q9Y2P5 |
PeptideAtlasi | Q9Y2P5 |
PRIDEi | Q9Y2P5 |
ProteomicsDBi | 85854 [Q9Y2P5-1] |
PTM databases
iPTMneti | Q9Y2P5 |
PhosphoSitePlusi | Q9Y2P5 |
Expressioni
Tissue specificityi
Predominantly expressed in liver.1 Publication
Gene expression databases
Bgeei | ENSG00000083807, Expressed in right lobe of liver and 172 other tissues |
ExpressionAtlasi | Q9Y2P5, baseline and differential |
Genevisiblei | Q9Y2P5, HS |
Organism-specific databases
HPAi | ENSG00000083807, Tissue enriched (liver) |
Interactioni
Binary interactionsi
Q9Y2P5
With | #Exp. | IntAct |
---|---|---|
MEOX2 [Q6FHY5] | 3 | EBI-12176609,EBI-16439278 |
Protein-protein interaction databases
BioGRIDi | 116191, 2 interactors |
IntActi | Q9Y2P5, 1 interactor |
STRINGi | 9606.ENSP00000263093 |
Miscellaneous databases
RNActi | Q9Y2P5, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1179, Eukaryota |
GeneTreei | ENSGT00940000157947 |
HOGENOMi | CLU_000022_46_2_1 |
InParanoidi | Q9Y2P5 |
OMAi | GATFFTY |
OrthoDBi | 298283at2759 |
PhylomeDBi | Q9Y2P5 |
TreeFami | TF313430 |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf IPR030302, FATP5 |
PANTHERi | PTHR43107:SF4, PTHR43107:SF4, 1 hit |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9Y2P5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGVRQQLALL LLLLLLLWGL GQPVWPVAVA LTLRWLLGDP TCCVLLGLAM
60 70 80 90 100
LARPWLGPWV PHGLSLAAAA LALTLLPARL PPGLRWLPAD VIFLAKILHL
110 120 130 140 150
GLKIRGCLSR QPPDTFVDAF ERRARAQPGR ALLVWTGPGA GSVTFGELDA
160 170 180 190 200
RACQAAWALK AELGDPASLC AGEPTALLVL ASQAVPALCM WLGLAKLGCP
210 220 230 240 250
TAWINPHGRG MPLAHSVLSS GARVLVVDPD LRESLEEILP KLQAENIRCF
260 270 280 290 300
YLSHTSPTPG VGALGAALDA APSHPVPADL RAGITWRSPA LFIYTSGTTG
310 320 330 340 350
LPKPAILTHE RVLQMSKMLS LSGATADDVV YTVLPLYHVM GLVVGILGCL
360 370 380 390 400
DLGATCVLAP KFSTSCFWDD CRQHGVTVIL YVGELLRYLC NIPQQPEDRT
410 420 430 440 450
HTVRLAMGNG LRADVWETFQ QRFGPIRIWE VYGSTEGNMG LVNYVGRCGA
460 470 480 490 500
LGKMSCLLRM LSPFELVQFD MEAAEPVRDN QGFCIPVGLG EPGLLLTKVV
510 520 530 540 550
SQQPFVGYRG PRELSERKLV RNVRQSGDVY YNTGDVLAMD REGFLYFRDR
560 570 580 590 600
LGDTFRWKGE NVSTHEVEGV LSQVDFLQQV NVYGVCVPGC EGKVGMAAVQ
610 620 630 640 650
LAPGQTFDGE KLYQHVRAWL PAYATPHFIR IQDAMEVTST FKLMKTRLVR
660 670 680 690
EGFNVGIVVD PLFVLDNRAQ SFRPLTAEMY QAVCEGTWRL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM0R075 | M0R075_HUMAN | Bile acyl-CoA synthetase | SLC27A5 | 95 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048243 | 50 | M → T. Corresponds to variant dbSNP:rs35350976EnsemblClinVar. | 1 | |
Natural variantiVAR_048244 | 53 | R → W. Corresponds to variant dbSNP:rs34415062EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055810 | 146 – 229 | Missing in isoform 2. 1 PublicationAdd BLAST | 84 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF064255 mRNA Translation: AAD29444.1 AK123036 mRNA Translation: BAG53858.1 AK298446 mRNA Translation: BAG60663.1 AC012313 Genomic DNA No translation available. CH471135 Genomic DNA Translation: EAW72601.1 |
CCDSi | CCDS12983.1 [Q9Y2P5-1] CCDS82415.1 [Q9Y2P5-2] |
RefSeqi | NP_001308125.1, NM_001321196.1 [Q9Y2P5-2] NP_036386.1, NM_012254.2 [Q9Y2P5-1] |
Genome annotation databases
Ensembli | ENST00000263093; ENSP00000263093; ENSG00000083807 [Q9Y2P5-1] ENST00000601355; ENSP00000470368; ENSG00000083807 [Q9Y2P5-2] |
GeneIDi | 10998 |
KEGGi | hsa:10998 |
UCSCi | uc002qtc.3, human [Q9Y2P5-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF064255 mRNA Translation: AAD29444.1 AK123036 mRNA Translation: BAG53858.1 AK298446 mRNA Translation: BAG60663.1 AC012313 Genomic DNA No translation available. CH471135 Genomic DNA Translation: EAW72601.1 |
CCDSi | CCDS12983.1 [Q9Y2P5-1] CCDS82415.1 [Q9Y2P5-2] |
RefSeqi | NP_001308125.1, NM_001321196.1 [Q9Y2P5-2] NP_036386.1, NM_012254.2 [Q9Y2P5-1] |
3D structure databases
SMRi | Q9Y2P5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116191, 2 interactors |
IntActi | Q9Y2P5, 1 interactor |
STRINGi | 9606.ENSP00000263093 |
Chemistry databases
ChEMBLi | CHEMBL5196 |
SwissLipidsi | SLP:000000429 SLP:000001315 [Q9Y2P5-1] |
Protein family/group databases
TCDBi | 4.C.1.1.13, the fatty acid transporter (fat) family |
PTM databases
iPTMneti | Q9Y2P5 |
PhosphoSitePlusi | Q9Y2P5 |
Polymorphism and mutation databases
BioMutai | SLC27A5 |
DMDMi | 74739456 |
Proteomic databases
jPOSTi | Q9Y2P5 |
MassIVEi | Q9Y2P5 |
PaxDbi | Q9Y2P5 |
PeptideAtlasi | Q9Y2P5 |
PRIDEi | Q9Y2P5 |
ProteomicsDBi | 85854 [Q9Y2P5-1] |
Protocols and materials databases
Antibodypediai | 1943, 294 antibodies |
DNASUi | 10998 |
Genome annotation databases
Ensembli | ENST00000263093; ENSP00000263093; ENSG00000083807 [Q9Y2P5-1] ENST00000601355; ENSP00000470368; ENSG00000083807 [Q9Y2P5-2] |
GeneIDi | 10998 |
KEGGi | hsa:10998 |
UCSCi | uc002qtc.3, human [Q9Y2P5-1] |
Organism-specific databases
CTDi | 10998 |
DisGeNETi | 10998 |
EuPathDBi | HostDB:ENSG00000083807.9 |
GeneCardsi | SLC27A5 |
HGNCi | HGNC:10999, SLC27A5 |
HPAi | ENSG00000083807, Tissue enriched (liver) |
MalaCardsi | SLC27A5 |
MIMi | 603314, gene |
neXtProti | NX_Q9Y2P5 |
OpenTargetsi | ENSG00000083807 |
Orphaneti | 276066, Bile acid CoA ligase deficiency and defective amidation |
PharmGKBi | PA35873 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1179, Eukaryota |
GeneTreei | ENSGT00940000157947 |
HOGENOMi | CLU_000022_46_2_1 |
InParanoidi | Q9Y2P5 |
OMAi | GATFFTY |
OrthoDBi | 298283at2759 |
PhylomeDBi | Q9Y2P5 |
TreeFami | TF313430 |
Enzyme and pathway databases
PathwayCommonsi | Q9Y2P5 |
Reactomei | R-HSA-159418, Recycling of bile acids and salts R-HSA-193368, Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-HSA-193775, Synthesis of bile acids and bile salts via 24-hydroxycholesterol |
Miscellaneous databases
BioGRID-ORCSi | 10998, 15 hits in 844 CRISPR screens |
ChiTaRSi | SLC27A5, human |
GeneWikii | SLC27A5 |
GenomeRNAii | 10998 |
Pharosi | Q9Y2P5, Tbio |
PROi | PR:Q9Y2P5 |
RNActi | Q9Y2P5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000083807, Expressed in right lobe of liver and 172 other tissues |
ExpressionAtlasi | Q9Y2P5, baseline and differential |
Genevisiblei | Q9Y2P5, HS |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf IPR030302, FATP5 |
PANTHERi | PTHR43107:SF4, PTHR43107:SF4, 1 hit |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S27A5_HUMAN | |
Accessioni | Q9Y2P5Primary (citable) accession number: Q9Y2P5 Secondary accession number(s): B3KVP6, B4DPQ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | November 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations