SLC27A5

Functionⁱ

Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.2 Publications

Catalytic activityⁱ

ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA.

ATP + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	292 – 303	AMPSequence analysisAdd BLAST		12

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
cholate-CoA ligase activity Source: UniProtKB-EC
fatty acid transmembrane transporter activity Source: Ensembl
long-chain fatty acid-CoA ligase activity Source: Reactome
protein-containing complex binding Source: Ensembl
very long-chain fatty acid-CoA ligase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 10479480
- 11980911

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

bile acid and bile salt transport Source: Reactome
bile acid biosynthetic process
Source: UniProtKB
Inferred from direct assayⁱ
- 11980911
ketone body biosynthetic process Source: Ensembl
plasma membrane long-chain fatty acid transport Source: Ensembl
triglyceride mobilization Source: Ensembl
very long-chain fatty acid metabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 10479480

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ligase
Biological process	Fatty acid metabolism, Lipid metabolism
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-HSA-159418 Recycling of bile acids and salts R-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-HSA-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol

Reactomeⁱ

R-HSA-159418 Recycling of bile acids and salts
R-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-HSA-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol

Protein family/group databases

TCDBⁱ	4.C.1.1.13 the fatty acid transporter (fat) family

TCDBⁱ

4.C.1.1.13 the fatty acid transporter (fat) family

Chemistry databases

SwissLipidsⁱ	SLP:000000429 SLP:000001315 [Q9Y2P5-1]

SwissLipidsⁱ

SLP:000000429
SLP:000001315 [Q9Y2P5-1]

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Bile acyl-CoA synthetase (EC:6.2.1.7) Short name: BACS Alternative name(s): Bile acid-CoA ligase Short name: BA-CoA ligase Short name: BAL Cholate--CoA ligase Fatty acid transport protein 5 Short name: FATP-5 Fatty-acid-coenzyme A ligase, very long-chain 3 Solute carrier family 27 member 5 Very long-chain acyl-CoA synthetase homolog 2 Short name: VLCS-H2 Short name: VLCSH2 Very long-chain acyl-CoA synthetase-related protein Short name: VLACS-related Short name: VLACSR
Gene namesⁱ	Name:SLC27A5 Synonyms:ACSB, ACSVL6, FACVL3, FATP5
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 19

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000083807.9
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:10999 SLC27A5
MIMⁱ	603314 gene
neXtProtⁱ	NX_Q9Y2P5

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 30	Cytoplasmic1 PublicationAdd BLAST	30
Transmembraneⁱ	31 – 51	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	56 – 76	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	77 – 690	Cytoplasmic1 PublicationAdd BLAST	614

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Membrane

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	10998
MalaCards human disease database More...MalaCardsⁱ	SLC27A5
Open Targets More...OpenTargetsⁱ	ENSG00000083807
PharmGKBⁱ	PA35873

Chemistry databases

ChEMBLⁱ	CHEMBL5196

ChEMBLⁱ

CHEMBL5196

Polymorphism and mutation databases

BioMutaⁱ	SLC27A5
DMDMⁱ	74739456

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000193213	1 – 690	Bile acyl-CoA synthetaseAdd BLAST		690

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	501	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q9Y2P5
PeptideAtlas More...PeptideAtlasⁱ	Q9Y2P5
PRIDEⁱ	Q9Y2P5
ProteomicsDBⁱ	85854

PTM databases

iPTMnetⁱ	Q9Y2P5
PhosphoSitePlusⁱ	Q9Y2P5

Expressionⁱ

Tissue specificityⁱ

Predominantly expressed in liver.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000083807 Expressed in 154 organ(s), highest expression level in right lobe of liver
CleanExⁱ	HS_SLC27A5
ExpressionAtlasⁱ	Q9Y2P5 baseline and differential
Genevisibleⁱ	Q9Y2P5 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB056160 CAB068239 HPA007292

HPAⁱ

CAB056160
CAB068239
HPA007292

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	116191, 1 interactor
IntActⁱ	Q9Y2P5, 1 interactor
STRINGⁱ	9606.ENSP00000263093

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9Y2P5
SMRⁱ	Q9Y2P5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ATP-dependent AMP-binding enzyme family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG1179 Eukaryota ENOG410XQ8T LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074420
HOGENOMⁱ	HOG000044189
HOVERGENⁱ	HBG005642
InParanoidⁱ	Q9Y2P5
KEGG Orthology (KO) More...KOⁱ	K08748
OMAⁱ	DCRQHGV
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0B76
PhylomeDBⁱ	Q9Y2P5
TreeFamⁱ	TF313430

Family and domain databases

InterProⁱ	View protein in InterPro IPR025110 AMP-bd_C IPR020845 AMP-binding_CS IPR000873 AMP-dep_Synth/Lig IPR030302 FATP5
PANTHERⁱ	PTHR43107:SF4 PTHR43107:SF4, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00501 AMP-binding, 1 hit PF13193 AMP-binding_C, 1 hit
PROSITEⁱ	View protein in PROSITE PS00455 AMP_BINDING, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.ⁱShow all

Isoform 1 (identifier: Q9Y2P5-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVRQQLALL LLLLLLLWGL GQPVWPVAVA LTLRWLLGDP TCCVLLGLAM 
        60         70         80         90        100
LARPWLGPWV PHGLSLAAAA LALTLLPARL PPGLRWLPAD VIFLAKILHL 
       110        120        130        140        150
GLKIRGCLSR QPPDTFVDAF ERRARAQPGR ALLVWTGPGA GSVTFGELDA 
       160        170        180        190        200
RACQAAWALK AELGDPASLC AGEPTALLVL ASQAVPALCM WLGLAKLGCP 
       210        220        230        240        250
TAWINPHGRG MPLAHSVLSS GARVLVVDPD LRESLEEILP KLQAENIRCF 
       260        270        280        290        300
YLSHTSPTPG VGALGAALDA APSHPVPADL RAGITWRSPA LFIYTSGTTG 
       310        320        330        340        350
LPKPAILTHE RVLQMSKMLS LSGATADDVV YTVLPLYHVM GLVVGILGCL 
       360        370        380        390        400
DLGATCVLAP KFSTSCFWDD CRQHGVTVIL YVGELLRYLC NIPQQPEDRT 
       410        420        430        440        450
HTVRLAMGNG LRADVWETFQ QRFGPIRIWE VYGSTEGNMG LVNYVGRCGA 
       460        470        480        490        500
LGKMSCLLRM LSPFELVQFD MEAAEPVRDN QGFCIPVGLG EPGLLLTKVV 
       510        520        530        540        550
SQQPFVGYRG PRELSERKLV RNVRQSGDVY YNTGDVLAMD REGFLYFRDR 
       560        570        580        590        600
LGDTFRWKGE NVSTHEVEGV LSQVDFLQQV NVYGVCVPGC EGKVGMAAVQ 
       610        620        630        640        650
LAPGQTFDGE KLYQHVRAWL PAYATPHFIR IQDAMEVTST FKLMKTRLVR 
       660        670        680        690
EGFNVGIVVD PLFVLDNRAQ SFRPLTAEMY QAVCEGTWRL

Length:690

Mass (Da):75,385

Last modified:November 1, 1999 - v1

Checksum:ⁱ011313424D794546

GO

Isoform 2 (identifier: Q9Y2P5-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
146-229: Missing.

« Hide

        10         20         30         40         50
MGVRQQLALL LLLLLLLWGL GQPVWPVAVA LTLRWLLGDP TCCVLLGLAM 
        60         70         80         90        100
LARPWLGPWV PHGLSLAAAA LALTLLPARL PPGLRWLPAD VIFLAKILHL 
       110        120        130        140        150
GLKIRGCLSR QPPDTFVDAF ERRARAQPGR ALLVWTGPGA GSVTFDLRES 
       160        170        180        190        200
LEEILPKLQA ENIRCFYLSH TSPTPGVGAL GAALDAAPSH PVPADLRAGI 
       210        220        230        240        250
TWRSPALFIY TSGTTGLPKP AILTHERVLQ MSKMLSLSGA TADDVVYTVL 
       260        270        280        290        300
PLYHVMGLVV GILGCLDLGA TCVLAPKFST SCFWDDCRQH GVTVILYVGE 
       310        320        330        340        350
LLRYLCNIPQ QPEDRTHTVR LAMGNGLRAD VWETFQQRFG PIRIWEVYGS 
       360        370        380        390        400
TEGNMGLVNY VGRCGALGKM SCLLRMLSPF ELVQFDMEAA EPVRDNQGFC 
       410        420        430        440        450
IPVGLGEPGL LLTKVVSQQP FVGYRGPREL SERKLVRNVR QSGDVYYNTG 
       460        470        480        490        500
DVLAMDREGF LYFRDRLGDT FRWKGENVST HEVEGVLSQV DFLQQVNVYG 
       510        520        530        540        550
VCVPGCEGKV GMAAVQLAPG QTFDGEKLYQ HVRAWLPAYA TPHFIRIQDA 
       560        570        580        590        600
MEVTSTFKLM KTRLVREGFN VGIVVDPLFV LDNRAQSFRP LTAEMYQAVC 

EGTWRL

Note: No experimental confirmation available.

Show »

Length:606

Mass (Da):66,846

Checksum:ⁱ4A49B281561FF8D6

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation
	M0R075	M0R075_HUMAN		Bile acyl-CoA synthetase Bile acyl-CoA synthetase	SLC27A5	95	Annotation score:

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_048243	50	M → T. Corresponds to variant dbSNP:rs35350976EnsemblClinVar.		1
Natural variantⁱVAR_048244	53	R → W. Corresponds to variant dbSNP:rs34415062EnsemblClinVar.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_055810	146 – 229	Missing in isoform 2. 1 PublicationAdd BLAST		84

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF064255 mRNA Translation: AAD29444.1 AK123036 mRNA Translation: BAG53858.1 AK298446 mRNA Translation: BAG60663.1 AC012313 Genomic DNA No translation available. CH471135 Genomic DNA Translation: EAW72601.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS12983.1 [Q9Y2P5-1] CCDS82415.1 [Q9Y2P5-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001308125.1, NM_001321196.1 [Q9Y2P5-2] NP_036386.1, NM_012254.2 [Q9Y2P5-1]
UniGeneⁱ	Hs.292177

Genome annotation databases

Ensemblⁱ	ENST00000263093; ENSP00000263093; ENSG00000083807 [Q9Y2P5-1] ENST00000601355; ENSP00000470368; ENSG00000083807 [Q9Y2P5-2]
GeneIDⁱ	10998
KEGGⁱ	hsa:10998
UCSC genome browser More...UCSCⁱ	uc002qtc.3 human [Q9Y2P5-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9Y2P5	Bile acyl-CoA synthetase		HUMAN		95	UniRef100_Q9Y2P5
SLC27A5 isoform 1		PANTR		95
Uncharacterized protein		GORGO		95

Protein	Similar proteins		Species	Score	Length	Source
Q9Y2P5	Bile acyl-CoA synthetase		HUMAN		95	UniRef90_Q9Y2P5
SLC27A5 isoform 1		PANTR		95
Uncharacterized protein		GORGO		95
Uncharacterized protein		MACFA		697
Solute carrier family 27 member 5		PANPA		690
+13
Q9Y2P5-2	Solute carrier family 27 member 5		MACMU		606	UniRef90_Q9Y2P5-2
SLC27A5 isoform 5		PONAB		606
SLC27A5 isoform 5		PANTR		606
UPI0002744C27		PANPA		606
UPI0005F4092E		COLAP		606
+2

Protein	Similar proteins		Species	Score	Length	Source
Q9Y2P5	Bile acyl-CoA synthetase		MOUSE		689	UniRef50_Q9Y2P5
Bile acyl-CoA synthetase		HUMAN		95
SLC27A5 isoform 1		PANTR		95
Uncharacterized protein		GORGO		95
Uncharacterized protein		MACFA		697
+76
Q9Y2P5-2	Solute carrier family 27 member 5		MACMU		606	UniRef50_Q9Y2P5-2
SLC27A5 isoform 5		PANTR		606
SLC27A5 isoform 5		PONAB		606
Bile acyl-CoA synthetase		TUPCH		639
Uncharacterized protein		MONDO		653
+11

Entry informationⁱ

Entry nameⁱ	S27A5_HUMAN
Accessionⁱ	Q9Y2P5Primary (citable) accession number: Q9Y2P5 Secondary accession number(s): B3KVP6, B4DPQ1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
	Last sequence update:	November 1, 1999
	Last modified:	September 12, 2018
	This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9Y2P5 (S27A5_HUMAN)

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Bile acyl-CoA synthetase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

Chemistry databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Endoplasmic reticulum

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Computationally mapped potential isoform sequencesⁱ

Keywords - Coding sequence diversityⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ