UniProtKB - Q9Y2M5 (KLH20_HUMAN)
Kelch-like protein 20
KLHL20
Functioni
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- interferon-gamma binding Source: UniProtKB
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- cytoskeleton organization Source: ProtInc
- Golgi to endosome transport Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- post-translational protein modification Source: Reactome
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein K33-linked ubiquitination Source: UniProtKB
- protein transport Source: UniProtKB-KW
- protein ubiquitination Source: UniProtKB
- response to interferon-alpha Source: UniProtKB
Keywordsi
Molecular function | Actin-binding |
Biological process | Protein transport, Transport, Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | Q9Y2M5 |
Reactomei | R-HSA-8951664, Neddylation R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Kelch-like protein 20Alternative name(s): Kelch-like ECT2-interacting protein Kelch-like protein X |
Gene namesi | Name:KLHL20 Synonyms:KLEIP, KLHLX |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25056, KLHL20 |
MIMi | 617679, gene |
neXtProti | NX_Q9Y2M5 |
VEuPathDBi | HostDB:ENSG00000076321.10 |
Subcellular locationi
Nucleus
Golgi apparatus
Other locations
- perinuclear region
- axon By similarity
- dendrite By similarity
Note: Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination (PubMed:20389280).1 Publication
Cytoskeleton
- actin cytoskeleton Source: ProtInc
Cytosol
- cytosol Source: HPA
Golgi apparatus
- Golgi apparatus Source: HPA
- trans-Golgi network Source: UniProtKB
Nucleus
- PML body Source: UniProtKB
Other locations
- axon Source: UniProtKB-SubCell
- Cul3-RING ubiquitin ligase complex Source: UniProtKB
- cytoplasm Source: UniProtKB
- dendrite Source: UniProtKB-SubCell
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell projection, Cytoplasm, Golgi apparatus, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 109 | V → A in KLHL20m6; abolishes interaction with CUL3; when associated with A-111; A-113; A-146; A-148 and A-150. 4 Publications | 1 | |
Mutagenesisi | 111 | I → A in KLHL20m6; abolishes interaction with CUL3; when associated with A-109; A-113; A-146; A-148 and A-150. 4 Publications | 1 | |
Mutagenesisi | 113 | D → A in KLHL20m6; abolishes interaction with CUL3; when associated with A-109; A-111; A-146; A-148 and A-150. 4 Publications | 1 | |
Mutagenesisi | 146 | C → A in KLHL20m6; abolishes interaction with CUL3; when associated with A-109; A-111; A-113; A-148 and A-150. 4 Publications | 1 | |
Mutagenesisi | 148 | L → A in KLHL20m6; abolishes interaction with CUL3; when associated with A-109; A-111; A-113; A-146 and A-150. 4 Publications | 1 | |
Mutagenesisi | 150 | L → A in KLHL20m6; abolishes interaction with CUL3; when associated with A-109; A-111; A-113; A-146 and A-148. 4 Publications | 1 |
Organism-specific databases
DisGeNETi | 27252 |
OpenTargetsi | ENSG00000076321 |
PharmGKBi | PA134982126 |
Miscellaneous databases
Pharosi | Q9Y2M5, Tbio |
Genetic variation databases
BioMutai | KLHL20 |
DMDMi | 257051084 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119123 | 1 – 609 | Kelch-like protein 20Add BLAST | 609 |
Proteomic databases
EPDi | Q9Y2M5 |
MassIVEi | Q9Y2M5 |
PaxDbi | Q9Y2M5 |
PeptideAtlasi | Q9Y2M5 |
PRIDEi | Q9Y2M5 |
ProteomicsDBi | 5204 85844 [Q9Y2M5-1] |
PTM databases
iPTMneti | Q9Y2M5 |
PhosphoSitePlusi | Q9Y2M5 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSG00000076321, Expressed in secondary oocyte and 235 other tissues |
ExpressionAtlasi | Q9Y2M5, baseline and differential |
Genevisiblei | Q9Y2M5, HS |
Organism-specific databases
HPAi | ENSG00000076321, Low tissue specificity |
Interactioni
Subunit structurei
Component of the BCR(KLHL20) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL20 and RBX1.
Interacts with PDZ-RhoGEF/ARHGEF11, DAPK1, PML and CORO7.
Interacts with F-actin.
Interacts with IFN-gamma (IFNG).
Interacts (via kelch repeats) with IVNS1ABP (via kelch repeats); this interaction blocks the assembly of CUL3-KLHL20 complex (PubMed:25619834).
7 PublicationsBinary interactionsi
Hide detailsQ9Y2M5
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- interferon-gamma binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 118100, 107 interactors |
CORUMi | Q9Y2M5 |
IntActi | Q9Y2M5, 99 interactors |
MINTi | Q9Y2M5 |
STRINGi | 9606.ENSP00000209884 |
Miscellaneous databases
RNActi | Q9Y2M5, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9Y2M5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 68 – 135 | BTBPROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 170 – 272 | BACKAdd BLAST | 103 | |
Repeati | 319 – 365 | Kelch 1Add BLAST | 47 | |
Repeati | 367 – 413 | Kelch 2Add BLAST | 47 | |
Repeati | 414 – 460 | Kelch 3Add BLAST | 47 | |
Repeati | 462 – 507 | Kelch 4Add BLAST | 46 | |
Repeati | 509 – 554 | Kelch 5Add BLAST | 46 | |
Repeati | 556 – 601 | Kelch 6Add BLAST | 46 |
Keywords - Domaini
Kelch repeat, RepeatPhylogenomic databases
eggNOGi | KOG4441, Eukaryota |
GeneTreei | ENSGT00940000155161 |
HOGENOMi | CLU_004253_14_2_1 |
InParanoidi | Q9Y2M5 |
OMAi | NSWSPIV |
OrthoDBi | 731760at2759 |
PhylomeDBi | Q9Y2M5 |
TreeFami | TF329218 |
Family and domain databases
Gene3Di | 2.120.10.80, 2 hits |
InterProi | View protein in InterPro IPR011705, BACK IPR017096, BTB-kelch_protein IPR000210, BTB/POZ_dom IPR015915, Kelch-typ_b-propeller IPR006652, Kelch_1 IPR030594, KLHL17/KLHL20 IPR011333, SKP1/BTB/POZ_sf |
PANTHERi | PTHR24412:SF454, PTHR24412:SF454, 2 hits |
Pfami | View protein in Pfam PF07707, BACK, 1 hit PF00651, BTB, 1 hit PF01344, Kelch_1, 6 hits |
PIRSFi | PIRSF037037, Kelch-like_protein_gigaxonin, 1 hit |
SMARTi | View protein in SMART SM00875, BACK, 1 hit SM00225, BTB, 1 hit SM00612, Kelch, 6 hits |
SUPFAMi | SSF117281, SSF117281, 1 hit SSF54695, SSF54695, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEGKPMRRCT NIRPGETGMD VTSRCTLGDP NKLPEGVPQP ARMPYISDKH
60 70 80 90 100
PRQTLEVINL LRKHRELCDV VLVVGAKKIY AHRVILSACS PYFRAMFTGE
110 120 130 140 150
LAESRQTEVV IRDIDERAME LLIDFAYTSQ ITVEEGNVQT LLPAACLLQL
160 170 180 190 200
AEIQEACCEF LKRQLDPSNC LGIRAFADTH SCRELLRIAD KFTQHNFQEV
210 220 230 240 250
MESEEFMLLP ANQLIDIISS DELNVRSEEQ VFNAVMAWVK YSIQERRPQL
260 270 280 290 300
PQVLQHVRLP LLSPKFLVGT VGSDPLIKSD EECRDLVDEA KNYLLLPQER
310 320 330 340 350
PLMQGPRTRP RKPIRCGEVL FAVGGWCSGD AISSVERYDP QTNEWRMVAS
360 370 380 390 400
MSKRRCGVGV SVLDDLLYAV GGHDGSSYLN SVERYDPKTN QWSSDVAPTS
410 420 430 440 450
TCRTSVGVAV LGGFLYAVGG QDGVSCLNIV ERYDPKENKW TRVASMSTRR
460 470 480 490 500
LGVAVAVLGG FLYAVGGSDG TSPLNTVERY NPQENRWHTI APMGTRRKHL
510 520 530 540 550
GCAVYQDMIY AVGGRDDTTE LSSAERYNPR TNQWSPVVAM TSRRSGVGLA
560 570 580 590 600
VVNGQLMAVG GFDGTTYLKT IEVFDPDANT WRLYGGMNYR RLGGGVGVIK
MTHCESHIW
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 171 | L → Q in BAG50912 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 321 | F → S in BAG50912 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 334 | S → N in BAG50912 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 396 | V → M in BAG50912 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 593 | G → W in BAA77027 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057026 | 1 – 10 | MEGKPMRRCT → MVHGRKANAQ in isoform 2. 1 Publication | 10 | |
Alternative sequenceiVSP_057027 | 11 – 199 | Missing in isoform 2. 1 PublicationAdd BLAST | 189 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ844466 mRNA Translation: CAH59617.1 AB026190 mRNA Translation: BAA77027.1 AK001430 mRNA Translation: BAG50912.1 AK300755 mRNA Translation: BAG62422.1 AL109921 Genomic DNA No translation available. BX248409 Genomic DNA No translation available. BC063418 mRNA Translation: AAH63418.1 |
CCDSi | CCDS1310.1 [Q9Y2M5-1] |
RefSeqi | NP_055273.2, NM_014458.3 [Q9Y2M5-1] XP_005245150.1, XM_005245093.3 [Q9Y2M5-1] |
Genome annotation databases
Ensembli | ENST00000209884; ENSP00000209884; ENSG00000076321 [Q9Y2M5-1] |
GeneIDi | 27252 |
KEGGi | hsa:27252 |
UCSCi | uc001gjc.5, human [Q9Y2M5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ844466 mRNA Translation: CAH59617.1 AB026190 mRNA Translation: BAA77027.1 AK001430 mRNA Translation: BAG50912.1 AK300755 mRNA Translation: BAG62422.1 AL109921 Genomic DNA No translation available. BX248409 Genomic DNA No translation available. BC063418 mRNA Translation: AAH63418.1 |
CCDSi | CCDS1310.1 [Q9Y2M5-1] |
RefSeqi | NP_055273.2, NM_014458.3 [Q9Y2M5-1] XP_005245150.1, XM_005245093.3 [Q9Y2M5-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5YQ4 | X-ray | 1.58 | A | 303-600 | [»] | |
6GY5 | X-ray | 1.09 | A | 303-605 | [»] | |
SMRi | Q9Y2M5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 118100, 107 interactors |
CORUMi | Q9Y2M5 |
IntActi | Q9Y2M5, 99 interactors |
MINTi | Q9Y2M5 |
STRINGi | 9606.ENSP00000209884 |
PTM databases
iPTMneti | Q9Y2M5 |
PhosphoSitePlusi | Q9Y2M5 |
Genetic variation databases
BioMutai | KLHL20 |
DMDMi | 257051084 |
Proteomic databases
EPDi | Q9Y2M5 |
MassIVEi | Q9Y2M5 |
PaxDbi | Q9Y2M5 |
PeptideAtlasi | Q9Y2M5 |
PRIDEi | Q9Y2M5 |
ProteomicsDBi | 5204 85844 [Q9Y2M5-1] |
Protocols and materials databases
Antibodypediai | 20562, 103 antibodies |
Genome annotation databases
Ensembli | ENST00000209884; ENSP00000209884; ENSG00000076321 [Q9Y2M5-1] |
GeneIDi | 27252 |
KEGGi | hsa:27252 |
UCSCi | uc001gjc.5, human [Q9Y2M5-1] |
Organism-specific databases
CTDi | 27252 |
DisGeNETi | 27252 |
GeneCardsi | KLHL20 |
HGNCi | HGNC:25056, KLHL20 |
HPAi | ENSG00000076321, Low tissue specificity |
MIMi | 617679, gene |
neXtProti | NX_Q9Y2M5 |
OpenTargetsi | ENSG00000076321 |
PharmGKBi | PA134982126 |
VEuPathDBi | HostDB:ENSG00000076321.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4441, Eukaryota |
GeneTreei | ENSGT00940000155161 |
HOGENOMi | CLU_004253_14_2_1 |
InParanoidi | Q9Y2M5 |
OMAi | NSWSPIV |
OrthoDBi | 731760at2759 |
PhylomeDBi | Q9Y2M5 |
TreeFami | TF329218 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q9Y2M5 |
Reactomei | R-HSA-8951664, Neddylation R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 27252, 7 hits in 1008 CRISPR screens |
ChiTaRSi | KLHL20, human |
GeneWikii | KLHL20 |
GenomeRNAii | 27252 |
Pharosi | Q9Y2M5, Tbio |
PROi | PR:Q9Y2M5 |
RNActi | Q9Y2M5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000076321, Expressed in secondary oocyte and 235 other tissues |
ExpressionAtlasi | Q9Y2M5, baseline and differential |
Genevisiblei | Q9Y2M5, HS |
Family and domain databases
Gene3Di | 2.120.10.80, 2 hits |
InterProi | View protein in InterPro IPR011705, BACK IPR017096, BTB-kelch_protein IPR000210, BTB/POZ_dom IPR015915, Kelch-typ_b-propeller IPR006652, Kelch_1 IPR030594, KLHL17/KLHL20 IPR011333, SKP1/BTB/POZ_sf |
PANTHERi | PTHR24412:SF454, PTHR24412:SF454, 2 hits |
Pfami | View protein in Pfam PF07707, BACK, 1 hit PF00651, BTB, 1 hit PF01344, Kelch_1, 6 hits |
PIRSFi | PIRSF037037, Kelch-like_protein_gigaxonin, 1 hit |
SMARTi | View protein in SMART SM00875, BACK, 1 hit SM00225, BTB, 1 hit SM00612, Kelch, 6 hits |
SUPFAMi | SSF117281, SSF117281, 1 hit SSF54695, SSF54695, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KLH20_HUMAN | |
Accessioni | Q9Y2M5Primary (citable) accession number: Q9Y2M5 Secondary accession number(s): B3KMA0 Q9H457 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | September 1, 2009 | |
Last modified: | April 7, 2021 | |
This is version 173 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references