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Protein

Zinc finger and BTB domain-containing protein 1

Gene

ZBTB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor (PubMed:20797634). Represses cAMP-responsive element (CRE)-mediated transcriptional activation (PubMed:21706167). In addition, has a role in translesion DNA synthesis. Requires for UV-inducible RAD18 loading, PCNA monoubiquitination, POLH recruitment to replication factories and efficient translesion DNA synthesis (PubMed:24657165). Plays a key role in the transcriptional regulation of T lymphocyte development (By similarity).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri216 – 242C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST27
Zinc fingeri448 – 470C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri533 – 558UBZ-type1 PublicationAdd BLAST26
Zinc fingeri578 – 600C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri606 – 628C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri634 – 656C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri662 – 684C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri686 – 709C2H2-type 8PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processDifferentiation, DNA damage, DNA repair, Immunity, Innate immunity, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 1
Gene namesi
Name:ZBTB1
Synonyms:KIAA0997
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000126804.13
HGNCiHGNC:20259 ZBTB1
MIMi616578 gene
neXtProtiNX_Q9Y2K1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi265K → R: Reduces sumoylation. Inhibits transcriptional repressive activity. Inhibits sumoylation and reduces transcriptional repressive activity; when associated with R-328. 1 Publication1
Mutagenesisi325I → S: Reduces sumoylation of Lys-328. 1
Mutagenesisi326I → N: Reduces sumoylation of Lys-328. 1
Mutagenesisi328K → R: Reduces sumoylation. Does not reduce transcriptional repressive activity. Inhibits sumoylation but does not reduce transcriptional repressive activity; when associated with R-265. 1 Publication1
Mutagenesisi536C → A: Abolishes binding to ubiquitin; Abolishes recruitment to DNA lesion sites. 1 Publication1
Mutagenesisi539C → A: Abolishes binding to ubiquitin; Abolishes recruitment to DNA lesion sites. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000126804
PharmGKBiPA128395769

Polymorphism and mutation databases

BioMutaiZBTB1
DMDMi46577710

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000477071 – 713Zinc finger and BTB domain-containing protein 1Add BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki3Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki200Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki205Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki260Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki265Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki275Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki283Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki303Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki316Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki328Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki340Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki346Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei355PhosphoserineCombined sources1
Modified residuei356PhosphothreonineCombined sources1
Cross-linki381Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki528Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki563Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Sumoylated with SUMO2 at Lys-328 and to a lesser extent at Lys-265. Sumoylation inhibits its transcriptional repression activity and regulates its subcellular localization.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Y2K1
PaxDbiQ9Y2K1
PeptideAtlasiQ9Y2K1
PRIDEiQ9Y2K1
ProteomicsDBi85818
85819 [Q9Y2K1-2]

PTM databases

iPTMnetiQ9Y2K1
PhosphoSitePlusiQ9Y2K1

Expressioni

Gene expression databases

BgeeiENSG00000126804 Expressed in 222 organ(s), highest expression level in sperm
CleanExiHS_ZBTB1
ExpressionAtlasiQ9Y2K1 baseline and differential
GenevisibleiQ9Y2K1 HS

Organism-specific databases

HPAiHPA050516

Interactioni

Subunit structurei

Homodimer. Homooligomer. Isoform 1 and isoform 2 can homodimerize. Heterodimer of isoform 1 and isoform 2. Interacts (via BTB domain) with TRIM28 (unphosphorylated or phosphorylated form) (PubMed:24657165).3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116556, 105 interactors
IntActiQ9Y2K1, 25 interactors
MINTiQ9Y2K1
STRINGi9606.ENSP00000378201

Structurei

3D structure databases

ProteinModelPortaliQ9Y2K1
SMRiQ9Y2K1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 91BTBPROSITE-ProRule annotationAdd BLAST68

Domaini

Both the BTB domain and C2H2-type motifs are necessary for transcriptional repression activity. The BTB domain is also necessary for oligomerization and efficient sumoylation. The hydrophobic cluster preceding Lys-328 enhanced sumoylation efficiency (PubMed:20797634).1 Publication
The UBZ-type zinc finger domain is required for targeting ZBTB1 to UV damage sites and for PCNA monoubiquitination. UBZ-type zinc finger domain mediates binding to 'Lys-63'-linked polyubiquitin chains (in vitro).1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri216 – 242C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST27
Zinc fingeri448 – 470C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri533 – 558UBZ-type1 PublicationAdd BLAST26
Zinc fingeri578 – 600C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri606 – 628C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri634 – 656C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri662 – 684C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri686 – 709C2H2-type 8PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00850000132316
HOGENOMiHOG000056456
HOVERGENiHBG057764
InParanoidiQ9Y2K1
KOiK10488
OMAiSHYLSKH
OrthoDBiEOG091G032Z
PhylomeDBiQ9Y2K1
TreeFamiTF332229

Family and domain databases

InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00651 BTB, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00355 ZnF_C2H2, 8 hits
SUPFAMiSSF54695 SSF54695, 1 hit
SSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y2K1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAKPSHSSYV LQQLNNQREW GFLCDCCIAI DDIYFQAHKA VLAACSSYFR
60 70 80 90 100
MFFMNHQHST AQLNLSNMKI SAECFDLILQ FMYLGKIMTA PSSFEQFKVA
110 120 130 140 150
MNYLQLYNVP DCLEDIQDAD CSSSKCSSSA SSKQNSKMIF GVRMYEDTVA
160 170 180 190 200
RNGNEANRWC AEPSSTVNTP HNREADEESL QLGNFPEPLF DVCKKSSVSK
210 220 230 240 250
LSTPKERVSR RFGRSFTCDS CGFGFSCEKL LDEHVLTCTN RHLYQNTRSY
260 270 280 290 300
HRIVDIRDGK DSNIKAEFGE KDSSKTFSAQ TDKYRGDTSQ AADDSASTTG
310 320 330 340 350
SRKSSTVESE IASEEKSRAA ERKRIIIKME PEDIPTDELK DFNIIKVTDK
360 370 380 390 400
DCNESTDNDE LEDEPEEPFY RYYVEEDVSI KKSGRKTLKP RMSVSADERG
410 420 430 440 450
GLENMRPPNN SSPVQEDAEN ASCELCGLTI TEEDLSSHYL AKHIENICAC
460 470 480 490 500
GKCGQILVKG RQLQEHAQRC GEPQDLTMNG LGNTEEKMDL EENPDEQSEI
510 520 530 540 550
RDMFVEMLDD FRDNHYQINS IQKKQLFKHS ACPFRCPNCG QRFETENLVV
560 570 580 590 600
EHMSSCLDQD MFKSAIMEEN ERDHRRKHFC NLCGKGFYQR CHLREHYTVH
610 620 630 640 650
TKEKQFVCQT CGKQFLRERQ LRLHNDMHKG MARYVCSICD QGNFRKHDHV
660 670 680 690 700
RHMISHLSAG ETICQVCFQI FPNNEQLEQH MDVHLYTCGI CGAKFNLRKD
710
MRSHYNAKHL KRT
Length:713
Mass (Da):82,016
Last modified:April 26, 2004 - v3
Checksum:i4B120DD45FA14305
GO
Isoform 2 (identifier: Q9Y2K1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     633-713: RYVCSICDQG...HYNAKHLKRT → SGEIGPSKPVEK

Show »
Length:644
Mass (Da):73,682
Checksum:i122AD0D3D565098F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V447G3V447_HUMAN
Zinc finger and BTB domain-containi...
ZBTB1
155Annotation score:
G3V2I8G3V2I8_HUMAN
Zinc finger and BTB domain-containi...
ZBTB1
130Annotation score:
G3V3N0G3V3N0_HUMAN
Zinc finger and BTB domain-containi...
ZBTB1
34Annotation score:

Sequence cautioni

The sequence BAA76841 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203T → N in BAA76841 (PubMed:10231032).Curated1
Sequence conflicti334I → T in AAH50719 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040976633 – 713RYVCS…HLKRT → SGEIGPSKPVEK in isoform 2. 1 PublicationAdd BLAST81

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK291743 mRNA Translation: BAF84432.1
AB023214 mRNA Translation: BAA76841.2 Different initiation.
AL049869 Genomic DNA No translation available.
BC050719 mRNA Translation: AAH50719.1
BX248777 mRNA Translation: CAD66584.1
CCDSiCCDS32097.1 [Q9Y2K1-2]
CCDS45126.1 [Q9Y2K1-1]
RefSeqiNP_001116801.1, NM_001123329.1 [Q9Y2K1-1]
NP_055765.2, NM_014950.2 [Q9Y2K1-2]
XP_005267467.1, XM_005267410.1 [Q9Y2K1-1]
XP_011534868.1, XM_011536566.2 [Q9Y2K1-1]
XP_011534870.1, XM_011536568.2 [Q9Y2K1-2]
XP_016876583.1, XM_017021094.1
XP_016876584.1, XM_017021095.1 [Q9Y2K1-1]
UniGeneiHs.605143

Genome annotation databases

EnsembliENST00000358738; ENSP00000351587; ENSG00000126804 [Q9Y2K1-2]
ENST00000554015; ENSP00000451000; ENSG00000126804 [Q9Y2K1-1]
GeneIDi22890
KEGGihsa:22890
UCSCiuc001xhh.5 human [Q9Y2K1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK291743 mRNA Translation: BAF84432.1
AB023214 mRNA Translation: BAA76841.2 Different initiation.
AL049869 Genomic DNA No translation available.
BC050719 mRNA Translation: AAH50719.1
BX248777 mRNA Translation: CAD66584.1
CCDSiCCDS32097.1 [Q9Y2K1-2]
CCDS45126.1 [Q9Y2K1-1]
RefSeqiNP_001116801.1, NM_001123329.1 [Q9Y2K1-1]
NP_055765.2, NM_014950.2 [Q9Y2K1-2]
XP_005267467.1, XM_005267410.1 [Q9Y2K1-1]
XP_011534868.1, XM_011536566.2 [Q9Y2K1-1]
XP_011534870.1, XM_011536568.2 [Q9Y2K1-2]
XP_016876583.1, XM_017021094.1
XP_016876584.1, XM_017021095.1 [Q9Y2K1-1]
UniGeneiHs.605143

3D structure databases

ProteinModelPortaliQ9Y2K1
SMRiQ9Y2K1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116556, 105 interactors
IntActiQ9Y2K1, 25 interactors
MINTiQ9Y2K1
STRINGi9606.ENSP00000378201

PTM databases

iPTMnetiQ9Y2K1
PhosphoSitePlusiQ9Y2K1

Polymorphism and mutation databases

BioMutaiZBTB1
DMDMi46577710

Proteomic databases

EPDiQ9Y2K1
PaxDbiQ9Y2K1
PeptideAtlasiQ9Y2K1
PRIDEiQ9Y2K1
ProteomicsDBi85818
85819 [Q9Y2K1-2]

Protocols and materials databases

DNASUi22890
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358738; ENSP00000351587; ENSG00000126804 [Q9Y2K1-2]
ENST00000554015; ENSP00000451000; ENSG00000126804 [Q9Y2K1-1]
GeneIDi22890
KEGGihsa:22890
UCSCiuc001xhh.5 human [Q9Y2K1-1]

Organism-specific databases

CTDi22890
EuPathDBiHostDB:ENSG00000126804.13
GeneCardsiZBTB1
H-InvDBiHIX0011734
HGNCiHGNC:20259 ZBTB1
HPAiHPA050516
MIMi616578 gene
neXtProtiNX_Q9Y2K1
OpenTargetsiENSG00000126804
PharmGKBiPA128395769
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00850000132316
HOGENOMiHOG000056456
HOVERGENiHBG057764
InParanoidiQ9Y2K1
KOiK10488
OMAiSHYLSKH
OrthoDBiEOG091G032Z
PhylomeDBiQ9Y2K1
TreeFamiTF332229

Miscellaneous databases

ChiTaRSiZBTB1 human
GenomeRNAii22890
PROiPR:Q9Y2K1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000126804 Expressed in 222 organ(s), highest expression level in sperm
CleanExiHS_ZBTB1
ExpressionAtlasiQ9Y2K1 baseline and differential
GenevisibleiQ9Y2K1 HS

Family and domain databases

InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00651 BTB, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00355 ZnF_C2H2, 8 hits
SUPFAMiSSF54695 SSF54695, 1 hit
SSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiZBTB1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2K1
Secondary accession number(s): A8K6S8, Q86SW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 26, 2004
Last modified: November 7, 2018
This is version 167 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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