UniProtKB - Q9Y2I6 (NINL_HUMAN)
Ninein-like protein
NINL
Functioni
Involved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis.
3 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 246 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 248 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 250 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 252 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 257 | CalciumPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
GO - Biological processi
- microtubule anchoring at centrosome Source: GO_Central
Keywordsi
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9Y2I6 |
Reactomei | R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-380259, Loss of Nlp from mitotic centrosomes R-HSA-380270, Recruitment of mitotic centrosome proteins and complexes R-HSA-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-380320, Recruitment of NuMA to mitotic centrosomes R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-8854518, AURKA Activation by TPX2 |
SignaLinki | Q9Y2I6 |
SIGNORi | Q9Y2I6 |
Names & Taxonomyi
Protein namesi | Recommended name: Ninein-like protein |
Gene namesi | Name:NINL Synonyms:KIAA0980, NLP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29163, NINL |
MIMi | 609580, gene |
neXtProti | NX_Q9Y2I6 |
VEuPathDBi | HostDB:ENSG00000101004 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- centrosome 1 Publication
Note: In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex (PubMed:16254247). During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm (PubMed:16254247).
Cytoskeleton
- centrosome Source: GO_Central
- microtubule Source: UniProtKB-KW
- microtubule cytoskeleton Source: HPA
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- intercellular bridge Source: HPA
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 495 – 497 | KEN → AAA: Disrupts binding to CDC20 and FZR1 and prevents ubiquitination and subsequent degradation of NINL; when associated with 633-A--A-636. 1 Publication | 3 | |
Mutagenesisi | 633 – 636 | RTQL → AAAA: Disrupts binding to CDC20 and FZR1 and prevents ubiquitination and subsequent degradation of NINL; when associated with 495-A--A-497. 1 Publication | 4 |
Organism-specific databases
DisGeNETi | 22981 |
OpenTargetsi | ENSG00000101004 |
PharmGKBi | PA165392435 |
Miscellaneous databases
Pharosi | Q9Y2I6, Tbio |
Genetic variation databases
BioMutai | NINL |
DMDMi | 118601093 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000259714 | 1 – 1382 | Ninein-like proteinAdd BLAST | 1382 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 148 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9Y2I6 |
jPOSTi | Q9Y2I6 |
MassIVEi | Q9Y2I6 |
MaxQBi | Q9Y2I6 |
PaxDbi | Q9Y2I6 |
PeptideAtlasi | Q9Y2I6 |
PRIDEi | Q9Y2I6 |
ProteomicsDBi | 85799 [Q9Y2I6-1] 85800 [Q9Y2I6-2] |
PTM databases
GlyGeni | Q9Y2I6, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9Y2I6 |
PhosphoSitePlusi | Q9Y2I6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000101004, Expressed in cortex of kidney and 212 other tissues |
ExpressionAtlasi | Q9Y2I6, baseline and differential |
Genevisiblei | Q9Y2I6, HS |
Organism-specific databases
HPAi | ENSG00000101004, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with gamma-tubulin and TUBGCP4.
Interacts with anaphase promoting complex/cyclosome (APC/C).
Interacts with CDC20 and FZR1.
Isoform 2 interacts with LCA5 and USH2A.
3 PublicationsBinary interactionsi
Q9Y2I6
Protein-protein interaction databases
BioGRIDi | 116630, 459 interactors |
ELMi | Q9Y2I6 |
IntActi | Q9Y2I6, 216 interactors |
MINTi | Q9Y2I6 |
STRINGi | 9606.ENSP00000278886 |
Miscellaneous databases
RNActi | Q9Y2I6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 42 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 41 – 76 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 196 – 231 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 233 – 268 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 144 – 164 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 857 – 969 | DisorderedSequence analysisAdd BLAST | 113 | |
Regioni | 982 – 1006 | DisorderedSequence analysisAdd BLAST | 25 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 384 – 424 | Sequence analysisAdd BLAST | 41 | |
Coiled coili | 484 – 579 | Sequence analysisAdd BLAST | 96 | |
Coiled coili | 616 – 699 | Sequence analysisAdd BLAST | 84 | |
Coiled coili | 1046 – 1375 | Sequence analysisAdd BLAST | 330 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 495 – 497 | KEN box | 3 | |
Motifi | 633 – 641 | D-box | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 149 – 164 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Domaini
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | ENOG502R2VA, Eukaryota |
GeneTreei | ENSGT00660000095541 |
HOGENOMi | CLU_001462_0_0_1 |
InParanoidi | Q9Y2I6 |
OMAi | TEIMVEQ |
OrthoDBi | 95801at2759 |
PhylomeDBi | Q9Y2I6 |
TreeFami | TF325139 |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
Pfami | View protein in Pfam PF13499, EF-hand_7, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 4 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDEEENHYVS QLREVYSSCD TTGTGFLDRQ ELTQLCLKLH LEQQLPVLLQ
60 70 80 90 100
TLLGNDHFAR VNFEEFKEGF VAVLSSNAGV RPSDEDSSSL ESAASSAIPP
110 120 130 140 150
KYVNGSKWYG RRSRPELCDA ATEARRVPEQ QTQASLKSHL WRSASLESVE
160 170 180 190 200
SPKSDEEAES TKEAQNELFE AQGQLQTWDS EDFGSPQKSC SPSFDTPESQ
210 220 230 240 250
IRGVWEELGV GSSGHLSEQE LAVVCQSVGL QGLEKEELED LFNKLDQDGD
260 270 280 290 300
GKVSLEEFQL GLFSHEPALL LESSTRVKPS KAWSHYQVPE ESGCHTTTTS
310 320 330 340 350
SLVSLCSSLR LFSSIDDGSG FAFPDQVLAM WTQEGIQNGR EILQSLDFSV
360 370 380 390 400
DEKVNLLELT WALDNELMTV DSAVQQAALA CYHQELSYQQ GQVEQLARER
410 420 430 440 450
DKARQDLERA EKRNLEFVKE MDDCHSTLEQ LTEKKIKHLE QGYRERLSLL
460 470 480 490 500
RSEVEAEREL FWEQAHRQRA ALEWDVGRLQ AEEAGLREKL TLALKENSRL
510 520 530 540 550
QKEIVEVVEK LSDSERLALK LQKDLEFVLK DKLEPQSAEL LAQEERFAAV
560 570 580 590 600
LKEYELKCRD LQDRNDELQA ELEGLWARLP KNRHSPSWSP DGRRRQLPGL
610 620 630 640 650
GPAGISFLGN SAPVSIETEL MMEQVKEHYQ DLRTQLETKV NYYEREIAAL
660 670 680 690 700
KRNFEKERKD MEQARRREVS VLEGQKADLE ELHEKSQEVI WGLQEQLQDT
710 720 730 740 750
ARGPEPEQMG LAPCCTQALC GLALRHHSHL QQIRREAEAE LSGELSGLGA
760 770 780 790 800
LPARRDLTLE LEEPPQGPLP RGSQRSEQLE LERALKLQPC ASEKRAQMCV
810 820 830 840 850
SLALEEEELE LARGKRVDGP SLEAEMQALP KDGLVAGSGQ EGTRGLLPLR
860 870 880 890 900
PGCGERPLAW LAPGDGRESE EAAGAGPRRR QAQDTEATQS PAPAPAPASH
910 920 930 940 950
GPSERWSRMQ PCGVDGDIVP KEPEPFGASA AGLEQPGARE LPLLGTERDA
960 970 980 990 1000
SQTQPRMWEP PLRPAASCRG QAERLQAIQE ERARSWSRGT QEQASEQQAR
1010 1020 1030 1040 1050
AEGALEPGCH KHSVEVARRG SLPSHLQLAD PQGSWQEQLA APEEGETKIA
1060 1070 1080 1090 1100
LEREKDDMET KLLHLEDVVR ALEKHVDLRE NDRLEFHRLS EENTLLKNDL
1110 1120 1130 1140 1150
GRVRQELEAA ESTHDAQRKE IEVLKKDKEK ACSEMEVLNR QNQNYKDQLS
1160 1170 1180 1190 1200
QLNVRVLQLG QEASTHQAQN EEHRVTIQML TQSLEEVVRS GQQQSDQIQK
1210 1220 1230 1240 1250
LRVELECLNQ EHQSLQLPWS ELTQTLEESQ DQVQGAHLRL RQAQAQHLQE
1260 1270 1280 1290 1300
VRLVPQDRVA ELHRLLSLQG EQARRRLDAQ REEHEKQLKA TEERVEEAEM
1310 1320 1330 1340 1350
ILKNMEMLLQ EKVDKLKEQF EKNTKSDLLL KELYVENAHL VRALQATEEK
1360 1370 1380
QRGAEKQSRL LEEKVRALNK LVSRIAPAAL SV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0Y2V7 | H0Y2V7_HUMAN | Ninein-like protein | NINL | 147 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 303 | V → L in BAH11644 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 958 – 959 | Missing in AAH36380 (PubMed:15489334).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_059700 | 79 | G → V. Corresponds to variant dbSNP:rs6115203Ensembl. | 1 | |
Natural variantiVAR_059701 | 191 | S → R. Corresponds to variant dbSNP:rs34585177Ensembl. | 1 | |
Natural variantiVAR_059702 | 276 | R → W. Corresponds to variant dbSNP:rs13044759Ensembl. | 1 | |
Natural variantiVAR_059703 | 296 | T → A. Corresponds to variant dbSNP:rs379538Ensembl. | 1 | |
Natural variantiVAR_058509 | 969 | R → G1 PublicationCorresponds to variant dbSNP:rs6115193Ensembl. | 1 | |
Natural variantiVAR_059704 | 973 | E → K. Corresponds to variant dbSNP:rs428801Ensembl. | 1 | |
Natural variantiVAR_061688 | 1077 | D → N. Corresponds to variant dbSNP:rs35666277Ensembl. | 1 | |
Natural variantiVAR_061689 | 1276 | R → C. Corresponds to variant dbSNP:rs41310175Ensembl. | 1 | |
Natural variantiVAR_058510 | 1366 | R → H1 PublicationCorresponds to variant dbSNP:rs17857107Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037883 | 735 – 1083 | Missing in isoform 2. 1 PublicationAdd BLAST | 349 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU718622 mRNA Translation: ACE78295.1 AB023197 mRNA Translation: BAA76824.1 Different initiation. AL031672 Genomic DNA No translation available. AL161802 Genomic DNA No translation available. BC036380 mRNA Translation: AAH36380.1 AK293991 mRNA Translation: BAH11644.1 Sequence problems. |
CCDSi | CCDS33452.1 [Q9Y2I6-1] CCDS82605.1 [Q9Y2I6-2] |
RefSeqi | NP_001305155.1, NM_001318226.1 [Q9Y2I6-2] NP_079452.3, NM_025176.5 [Q9Y2I6-1] |
Genome annotation databases
Ensembli | ENST00000278886.11; ENSP00000278886.6; ENSG00000101004.15 ENST00000422516.5; ENSP00000410431.1; ENSG00000101004.15 [Q9Y2I6-2] |
GeneIDi | 22981 |
KEGGi | hsa:22981 |
MANE-Selecti | ENST00000278886.11; ENSP00000278886.6; NM_025176.6; NP_079452.3 |
UCSCi | uc002wux.3, human [Q9Y2I6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU718622 mRNA Translation: ACE78295.1 AB023197 mRNA Translation: BAA76824.1 Different initiation. AL031672 Genomic DNA No translation available. AL161802 Genomic DNA No translation available. BC036380 mRNA Translation: AAH36380.1 AK293991 mRNA Translation: BAH11644.1 Sequence problems. |
CCDSi | CCDS33452.1 [Q9Y2I6-1] CCDS82605.1 [Q9Y2I6-2] |
RefSeqi | NP_001305155.1, NM_001318226.1 [Q9Y2I6-2] NP_079452.3, NM_025176.5 [Q9Y2I6-1] |
3D structure databases
AlphaFoldDBi | Q9Y2I6 |
SMRi | Q9Y2I6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116630, 459 interactors |
ELMi | Q9Y2I6 |
IntActi | Q9Y2I6, 216 interactors |
MINTi | Q9Y2I6 |
STRINGi | 9606.ENSP00000278886 |
PTM databases
GlyGeni | Q9Y2I6, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9Y2I6 |
PhosphoSitePlusi | Q9Y2I6 |
Genetic variation databases
BioMutai | NINL |
DMDMi | 118601093 |
Proteomic databases
EPDi | Q9Y2I6 |
jPOSTi | Q9Y2I6 |
MassIVEi | Q9Y2I6 |
MaxQBi | Q9Y2I6 |
PaxDbi | Q9Y2I6 |
PeptideAtlasi | Q9Y2I6 |
PRIDEi | Q9Y2I6 |
ProteomicsDBi | 85799 [Q9Y2I6-1] 85800 [Q9Y2I6-2] |
Protocols and materials databases
Antibodypediai | 628, 61 antibodies from 16 providers |
DNASUi | 22981 |
Genome annotation databases
Ensembli | ENST00000278886.11; ENSP00000278886.6; ENSG00000101004.15 ENST00000422516.5; ENSP00000410431.1; ENSG00000101004.15 [Q9Y2I6-2] |
GeneIDi | 22981 |
KEGGi | hsa:22981 |
MANE-Selecti | ENST00000278886.11; ENSP00000278886.6; NM_025176.6; NP_079452.3 |
UCSCi | uc002wux.3, human [Q9Y2I6-1] |
Organism-specific databases
CTDi | 22981 |
DisGeNETi | 22981 |
GeneCardsi | NINL |
HGNCi | HGNC:29163, NINL |
HPAi | ENSG00000101004, Low tissue specificity |
MIMi | 609580, gene |
neXtProti | NX_Q9Y2I6 |
OpenTargetsi | ENSG00000101004 |
PharmGKBi | PA165392435 |
VEuPathDBi | HostDB:ENSG00000101004 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502R2VA, Eukaryota |
GeneTreei | ENSGT00660000095541 |
HOGENOMi | CLU_001462_0_0_1 |
InParanoidi | Q9Y2I6 |
OMAi | TEIMVEQ |
OrthoDBi | 95801at2759 |
PhylomeDBi | Q9Y2I6 |
TreeFami | TF325139 |
Enzyme and pathway databases
PathwayCommonsi | Q9Y2I6 |
Reactomei | R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-380259, Loss of Nlp from mitotic centrosomes R-HSA-380270, Recruitment of mitotic centrosome proteins and complexes R-HSA-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-380320, Recruitment of NuMA to mitotic centrosomes R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-8854518, AURKA Activation by TPX2 |
SignaLinki | Q9Y2I6 |
SIGNORi | Q9Y2I6 |
Miscellaneous databases
BioGRID-ORCSi | 22981, 13 hits in 1080 CRISPR screens |
ChiTaRSi | NINL, human |
GeneWikii | NINL |
GenomeRNAii | 22981 |
Pharosi | Q9Y2I6, Tbio |
PROi | PR:Q9Y2I6 |
RNActi | Q9Y2I6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000101004, Expressed in cortex of kidney and 212 other tissues |
ExpressionAtlasi | Q9Y2I6, baseline and differential |
Genevisiblei | Q9Y2I6, HS |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
Pfami | View protein in Pfam PF13499, EF-hand_7, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NINL_HUMAN | |
Accessioni | Q9Y2I6Primary (citable) accession number: Q9Y2I6 Secondary accession number(s): A6NJN0 Q9NQE3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | October 31, 2006 | |
Last modified: | May 25, 2022 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot