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UniProtKB - Q9Y2I1 (NISCH_HUMAN)

Entry version 156 (23 Feb 2022)
Sequence version 3 (18 May 2010)
Previous versions | rss
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Protein

Nischarin

Gene

NISCH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity).

Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity).

Acts as a modulator of Rac-regulated signal transduction pathways (By similarity).

Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (By similarity).

Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation (By similarity).

Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation (By similarity).

Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells (By similarity).

Inhibits lamellipodia formation, when overexpressed (By similarity).

Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.

By similarity5 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionReceptor
Biological processApoptosis

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q9Y2I1

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-9013149, RAC1 GTPase cycle
R-HSA-9696264, RND3 GTPase cycle
R-HSA-9696270, RND2 GTPase cycle

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		Q9Y2I1

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Nischarin
Alternative name(s):
Imidazoline receptor 1
Short name:
I-1
Short name:
IR1
Imidazoline receptor antisera-selected protein
Short name:
hIRAS
Imidazoline-1 receptor
Short name:
I1R
Imidazoline-1 receptor candidate protein
Short name:
I-1 receptor candidate protein
Short name:
I1R candidate protein
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NISCH
Synonyms:IRAS, KIAA0975
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:18006, NISCH

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		615507, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q9Y2I1

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000010322

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endosome, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi49R → A: Inhibits targeting to endosomes. 1 Publication1
Mutagenesisi50Y → A: Inhibits targeting to endosomes. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		11188

Open Targets

More...OpenTargetsi		ENSG00000010322

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA31635

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q9Y2I1, Tclin

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL3923

Drug and drug target database

More...DrugBanki		DB08838, Agmatine
DB09242, Moxonidine
DB00697, Tizanidine

DrugCentral

More...DrugCentrali		Q9Y2I1

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		NISCH

Domain mapping of disease mutations (DMDM)

More...DMDMi		296439287

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003482652 – 1504NischarinAdd BLAST1503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylalanineCombined sources1
Modified residuei541PhosphoserineBy similarity1
Modified residuei543PhosphoserineBy similarity1
Modified residuei546PhosphoserineBy similarity1
Modified residuei1022PhosphoserineCombined sources1
Modified residuei1282PhosphothreonineBy similarity1
Modified residuei1284PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q9Y2I1

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q9Y2I1

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q9Y2I1

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q9Y2I1

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q9Y2I1

PeptideAtlas

More...PeptideAtlasi		Q9Y2I1

PRoteomics IDEntifications database

More...PRIDEi		Q9Y2I1

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		85789 [Q9Y2I1-1]
85790 [Q9Y2I1-2]
85791 [Q9Y2I1-3]
85792 [Q9Y2I1-4]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q9Y2I1

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q9Y2I1

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Isoform 1, isoform 3 and isoform 4 are expressed in brain. Isoform 1 is expressed in endocrine tissues.2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000010322, Expressed in right hemisphere of cerebellum and 241 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q9Y2I1, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q9Y2I1, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000010322, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homooligomer.

Interacts with GRB2.

Interacts with PIK3R1; probably associates with the PI3-kinase complex.

Interacts with IRS4.

Found in a complex with ITGA5 and PAK1.

Found in a complex with LIMK1 and PAK1.

Interacts with ITGA5 (via cytoplasmic domain); this interaction is direct.

Interacts with PAK1 (via kinase domain); this interaction is direct and is increased upon activation of PAK1 (By similarity).

Interacts with LIMK1 (via PDZ and kinase domain); this interaction is direct (By similarity).

Interacts with LIMK2; this interaction depends on LIMK2 activity (By similarity).

Interacts with RAC1 (activated state) (By similarity).

Interacts with STK11; this interaction may increase STK11 activity.

By similarity3 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		116358, 107 interactors

Protein interaction database and analysis system

More...IntActi		Q9Y2I1, 40 interactors

Molecular INTeraction database

More...MINTi		Q9Y2I1

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000339958

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		Q9Y2I1

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q9Y2I1, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11504
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q9Y2I1

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini11 – 121PXPROSITE-ProRule annotationAdd BLAST111
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati288 – 309LRR 1Add BLAST22
Repeati311 – 332LRR 2Add BLAST22
Repeati333 – 354LRR 3Add BLAST22
Repeati356 – 377LRR 4Add BLAST22
Repeati378 – 399LRR 5Add BLAST22
Repeati403 – 424LRR 6Add BLAST22

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni2 – 133Necessary for binding to phosphoinositide-3-P; not sufficient for targeting to endosomesAdd BLAST132
Regioni120 – 695Necessary for homooligomerization and targeting to endosomesAdd BLAST576
Regioni245 – 869Interaction with PAK1By similarityAdd BLAST625
Regioni463 – 501DisorderedSequence analysisAdd BLAST39
Regioni524 – 547DisorderedSequence analysisAdd BLAST24
Regioni554 – 573DisorderedSequence analysisAdd BLAST20
Regioni628 – 687DisorderedSequence analysisAdd BLAST60
Regioni660 – 869Interaction with LIMKBy similarityAdd BLAST210
Regioni709 – 807Interaction with ITGA5By similarityAdd BLAST99
Regioni1016 – 1104DisorderedSequence analysisAdd BLAST89

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili634 – 695Sequence analysisAdd BLAST62

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi463 – 477Basic and acidic residuesSequence analysisAdd BLAST15
Compositional biasi483 – 501Polar residuesSequence analysisAdd BLAST19
Compositional biasi633 – 651Acidic residuesSequence analysisAdd BLAST19
Compositional biasi661 – 687Acidic residuesSequence analysisAdd BLAST27

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Both the presence of the PX domain and the coiled coil region are necessary for its endosomal targeting.

Keywords - Domaini

Coiled coil, Leucine-rich repeat, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1259, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000156494

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_252294_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q9Y2I1

Identification of Orthologs from Complete Genome Data

More...OMAi		PIDKDFY

Database of Orthologous Groups

More...OrthoDBi		320361at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q9Y2I1

TreeFam database of animal gene trees

More...TreeFami		TF320547

Family and domain databases

Conserved Domains Database

More...CDDi		cd06875, PX_IRAS, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.30.1520.10, 1 hit
3.80.10.10, 2 hits

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR001611, Leu-rich_rpt
IPR032675, LRR_dom_sf
IPR037904, Nischarin_PX
IPR001683, PX_dom
IPR036871, PX_dom_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00787, PX, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00312, PX, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF64268, SSF64268, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51450, LRR, 6 hits
PS50195, PX, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (4+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y2I1-1) [UniParc]FASTAAdd to basket
Also known as: IRAS-1, IRAS-M

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATARTFGPE REAEPAKEAR VVGSELVDTY TVYIIQVTDG SHEWTVKHRY 
        60         70         80         90        100
SDFHDLHEKL VAERKIDKNL LPPKKIIGKN SRSLVEKREK DLEVYLQKLL 
       110        120        130        140        150
AAFPGVTPRV LAHFLHFHFY EINGITAALA EELFEKGEQL LGAGEVFAIG 
       160        170        180        190        200
PLQLYAVTEQ LQQGKPTCAS GDAKTDLGHI LDFTCRLKYL KVSGTEGPFG 
       210        220        230        240        250
TSNIQEQLLP FDLSIFKSLH QVEISHCDAK HIRGLVASKP TLATLSVRFS 
       260        270        280        290        300
ATSMKEVLVP EASEFDEWEP EGTTLEGPVT AVIPTWQALT TLDLSHNSVS 
       310        320        330        340        350
EIDESVKLIP KIEFLDLSHN GLLVVDNLQH LYNLVHLDLS YNKLSSLEGL 
       360        370        380        390        400
HTKLGNIKTL NLAGNLLESL SGLHKLYSLV NLDLRDNRIE QMEEVRSIGS 
       410        420        430        440        450
LPCLEHVSLL NNPLSIIPDY RTKVLAQFGE RASEVCLDDT VTTEKELDTV 
       460        470        480        490        500
EVLKAIQKAK EVKSKLSNPE KKGGEDSRLS AAPCIRPSSS PPTVAPASAS 
       510        520        530        540        550
LPQPILSNQG IMFVQEEALA SSLSSTDSLT PEHQPIAQGC SDSLESIPAG 
       560        570        580        590        600
QAASDDLRDV PGAVGGASPE HAEPEVQVVP GSGQIIFLPF TCIGYTATNQ 
       610        620        630        640        650
DFIQRLSTLI RQAIERQLPA WIEAANQREE GQGEQGEEED EEEEEEEDVA 
       660        670        680        690        700
ENRYFEMGPP DVEEEEGGGQ GEEEEEEEED EEAEEERLAL EWALGADEDF 
       710        720        730        740        750
LLEHIRILKV LWCFLIHVQG SIRQFAACLV LTDFGIAVFE IPHQESRGSS 
       760        770        780        790        800
QHILSSLRFV FCFPHGDLTE FGFLMPELCL VLKVRHSENT LFIISDAANL 
       810        820        830        840        850
HEFHADLRSC FAPQHMAMLC SPILYGSHTS LQEFLRQLLT FYKVAGGCQE 
       860        870        880        890        900
RSQGCFPVYL VYSDKRMVQT AAGDYSGNIE WASCTLCSAV RRSCCAPSEA 
       910        920        930        940        950
VKSAAIPYWL LLTPQHLNVI KADFNPMPNR GTHNCRNRNS FKLSRVPLST 
       960        970        980        990       1000
VLLDPTRSCT QPRGAFADGH VLELLVGYRF VTAIFVLPHE KFHFLRVYNQ 
      1010       1020       1030       1040       1050
LRASLQDLKT VVIAKTPGTG GSPQGSFADG QPAERRASND QRPQEVPAEA 
      1060       1070       1080       1090       1100
LAPAPAEVPA PAPAAASASG PAKTPAPAEA STSALVPEET PVEAPAPPPA 
      1110       1120       1130       1140       1150
EAPAQYPSEH LIQATSEENQ IPSHLPACPS LRHVASLRGS AIIELFHSSI 
      1160       1170       1180       1190       1200
AEVENEELRH LMWSSVVFYQ TPGLEVTACV LLSTKAVYFV LHDGLRRYFS 
      1210       1220       1230       1240       1250
EPLQDFWHQK NTDYNNSPFH ISQCFVLKLS DLQSVNVGLF DQHFRLTGST 
      1260       1270       1280       1290       1300
PMQVVTCLTR DSYLTHCFLQ HLMVVLSSLE RTPSPEPVDK DFYSEFGNKT 
      1310       1320       1330       1340       1350
TGKMENYELI HSSRVKFTYP SEEEIGDLTF TVAQKMAEPE KAPALSILLY 
      1360       1370       1380       1390       1400
VQAFQVGMPP PGCCRGPLRP KTLLLTSSEI FLLDEDCVHY PLPEFAKEPP 
      1410       1420       1430       1440       1450
QRDRYRLDDG RRVRDLDRVL MGYQTYPQAL TLVFDDVQGH DLMGSVTLDH 
      1460       1470       1480       1490       1500
FGEVPGGPAR ASQGREVQWQ VFVPSAESRE KLISLLARQW EALCGRELPV 

ELTG
Length:1,504
Mass (Da):166,629
Last modified:May 18, 2010 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7761C7DA1FDC4E53
GO
Isoform 2 (identifier: Q9Y2I1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-511: Missing.

Show »
Length:993
Mass (Da):110,194
Checksum:iE0415818937AA523
GO
Isoform 3 (identifier: Q9Y2I1-3) [UniParc]FASTAAdd to basket
Also known as: IRAS-L

The sequence of this isoform differs from the canonical sequence as follows:
     511-583: IMFVQEEALA...PEVQVVPGSG → NRVCTLLLVE...CLLGEDSQLL
     584-1504: Missing.

Show »
Length:583
Mass (Da):63,997
Checksum:i83CED19440961191
GO
Isoform 4 (identifier: Q9Y2I1-4) [UniParc]FASTAAdd to basket
Also known as: IRAS-S

The sequence of this isoform differs from the canonical sequence as follows:
     512-515: MFVQ → LGDE
     516-1504: Missing.

Show »
Length:515
Mass (Da):56,867
Checksum:iB623BE4D42BBA51C
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J715C9J715_HUMAN
Nischarin
NISCH
583Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAA76819 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti927M → I in AAH56900 (PubMed:15489334).Curated1
Sequence conflicti1023P → A in BAA76819 (PubMed:10231032).Curated1
Sequence conflicti1123S → P in AAH38102 (PubMed:15489334).Curated1
Sequence conflicti1382L → F in AAH54494 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_046130299V → I6 PublicationsCorresponds to variant dbSNP:rs9856575Ensembl.1
Natural variantiVAR_0461311056A → V7 PublicationsCorresponds to variant dbSNP:rs887515Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0351311 – 511Missing in isoform 2. 1 PublicationAdd BLAST511
Alternative sequenceiVSP_035132511 – 583IMFVQ…VPGSG → NRVCTLLLVEPHSPAWAPWL GWGWGRGASTCFQQGTQGGG QCLLQAGPRGGTHGRGAWPD ASCCLLGEDSQLL in isoform 3. 1 PublicationAdd BLAST73
Alternative sequenceiVSP_035133512 – 515MFVQ → LGDE in isoform 4. 1 Publication4
Alternative sequenceiVSP_035134516 – 1504Missing in isoform 4. 1 PublicationAdd BLAST989
Alternative sequenceiVSP_035135584 – 1504Missing in isoform 3. 1 PublicationAdd BLAST921

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF082516 mRNA Translation: AAC33104.1
AB023192 mRNA Translation: BAA76819.1 Different initiation.
AK291398 mRNA Translation: BAF84087.1
AL117432 mRNA Translation: CAB55920.1
AC006208 Genomic DNA No translation available.
CH471055 Genomic DNA Translation: EAW65229.1
BC038102 mRNA Translation: AAH38102.1
BC054494 mRNA Translation: AAH54494.1
BC056900 mRNA Translation: AAH56900.1
AF058290 mRNA Translation: AAC33321.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS33767.1 [Q9Y2I1-1]
CCDS63651.1 [Q9Y2I1-4]
CCDS63652.1 [Q9Y2I1-3]

Protein sequence database of the Protein Information Resource

More...PIRi		T17230

NCBI Reference Sequences

More...RefSeqi		NP_001263222.1, NM_001276293.1
NP_001263223.1, NM_001276294.1 [Q9Y2I1-4]
NP_009115.2, NM_007184.3 [Q9Y2I1-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000345716; ENSP00000339958; ENSG00000010322
ENST00000420808; ENSP00000392484; ENSG00000010322 [Q9Y2I1-4]
ENST00000479054; ENSP00000418232; ENSG00000010322

Database of genes from NCBI RefSeq genomes

More...GeneIDi		11188

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:11188

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000345716.9; ENSP00000339958.4; NM_007184.4; NP_009115.3

UCSC genome browser

More...UCSCi		uc003ded.5, human [Q9Y2I1-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082516 mRNA Translation: AAC33104.1
AB023192 mRNA Translation: BAA76819.1 Different initiation.
AK291398 mRNA Translation: BAF84087.1
AL117432 mRNA Translation: CAB55920.1
AC006208 Genomic DNA No translation available.
CH471055 Genomic DNA Translation: EAW65229.1
BC038102 mRNA Translation: AAH38102.1
BC054494 mRNA Translation: AAH54494.1
BC056900 mRNA Translation: AAH56900.1
AF058290 mRNA Translation: AAC33321.1
CCDSiCCDS33767.1 [Q9Y2I1-1]
CCDS63651.1 [Q9Y2I1-4]
CCDS63652.1 [Q9Y2I1-3]
PIRiT17230
RefSeqiNP_001263222.1, NM_001276293.1
NP_001263223.1, NM_001276294.1 [Q9Y2I1-4]
NP_009115.2, NM_007184.3 [Q9Y2I1-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P0CX-ray2.27A/B18-124[»]
SMRiQ9Y2I1
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi116358, 107 interactors
IntActiQ9Y2I1, 40 interactors
MINTiQ9Y2I1
STRINGi9606.ENSP00000339958

Chemistry databases

BindingDBiQ9Y2I1
ChEMBLiCHEMBL3923
DrugBankiDB08838, Agmatine
DB09242, Moxonidine
DB00697, Tizanidine
DrugCentraliQ9Y2I1

PTM databases

iPTMnetiQ9Y2I1
PhosphoSitePlusiQ9Y2I1

Genetic variation databases

BioMutaiNISCH
DMDMi296439287

Proteomic databases

EPDiQ9Y2I1
jPOSTiQ9Y2I1
MassIVEiQ9Y2I1
MaxQBiQ9Y2I1
PaxDbiQ9Y2I1
PeptideAtlasiQ9Y2I1
PRIDEiQ9Y2I1
ProteomicsDBi85789 [Q9Y2I1-1]
85790 [Q9Y2I1-2]
85791 [Q9Y2I1-3]
85792 [Q9Y2I1-4]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		14512, 107 antibodies from 25 providers

The DNASU plasmid repository

More...DNASUi		11188

Genome annotation databases

EnsembliENST00000345716; ENSP00000339958; ENSG00000010322
ENST00000420808; ENSP00000392484; ENSG00000010322 [Q9Y2I1-4]
ENST00000479054; ENSP00000418232; ENSG00000010322
GeneIDi11188
KEGGihsa:11188
MANE-SelectiENST00000345716.9; ENSP00000339958.4; NM_007184.4; NP_009115.3
UCSCiuc003ded.5, human [Q9Y2I1-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		11188
DisGeNETi11188

GeneCards: human genes, protein and diseases

More...GeneCardsi		NISCH
HGNCiHGNC:18006, NISCH
HPAiENSG00000010322, Low tissue specificity
MIMi615507, gene
neXtProtiNX_Q9Y2I1
OpenTargetsiENSG00000010322
PharmGKBiPA31635
VEuPathDBiHostDB:ENSG00000010322

Human Unidentified Gene-Encoded large proteins database

More...HUGEi		Search...

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG1259, Eukaryota
GeneTreeiENSGT00940000156494
HOGENOMiCLU_252294_0_0_1
InParanoidiQ9Y2I1
OMAiPIDKDFY
OrthoDBi320361at2759
PhylomeDBiQ9Y2I1
TreeFamiTF320547

Enzyme and pathway databases

PathwayCommonsiQ9Y2I1
ReactomeiR-HSA-9013149, RAC1 GTPase cycle
R-HSA-9696264, RND3 GTPase cycle
R-HSA-9696270, RND2 GTPase cycle
SignaLinkiQ9Y2I1

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		11188, 59 hits in 1054 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		NISCH, human

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		NISCH

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		11188
PharosiQ9Y2I1, Tclin

Protein Ontology

More...PROi		PR:Q9Y2I1
RNActiQ9Y2I1, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000010322, Expressed in right hemisphere of cerebellum and 241 other tissues
ExpressionAtlasiQ9Y2I1, baseline and differential
GenevisibleiQ9Y2I1, HS

Family and domain databases

CDDicd06875, PX_IRAS, 1 hit
Gene3Di3.30.1520.10, 1 hit
3.80.10.10, 2 hits
InterProiView protein in InterPro
IPR001611, Leu-rich_rpt
IPR032675, LRR_dom_sf
IPR037904, Nischarin_PX
IPR001683, PX_dom
IPR036871, PX_dom_sf
PfamiView protein in Pfam
PF00787, PX, 1 hit
SMARTiView protein in SMART
SM00312, PX, 1 hit
SUPFAMiSSF64268, SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS51450, LRR, 6 hits
PS50195, PX, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNISCH_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9Y2I1
Secondary accession number(s): C9J245
, Q6PGP3, Q6PIB4, Q7L8M3, Q7Z2X6, Q9UES6, Q9UEU4, Q9UFW3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 18, 2010
Last modified: February 23, 2022
This is version 156 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. UniProtKB entry view manual
    User manual for the UniProtKB entry view
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with genetic variants
    List of human entries with genetic variants
  4. Human variants curated from literature reports
    Index of human variants curated from literature reports
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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