Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hepatocyte nuclear factor 3-beta

Gene

FOXA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi159 – 252Fork-headPROSITE-ProRule annotationAdd BLAST94

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Chromatin regulator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-210745 Regulation of gene expression in beta cells
SignaLinkiQ9Y261
SIGNORiQ9Y261

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte nuclear factor 3-beta
Short name:
HNF-3-beta
Short name:
HNF-3B
Alternative name(s):
Forkhead box protein A2
Transcription factor 3B
Short name:
TCF-3B
Gene namesi
Name:FOXA2
Synonyms:HNF3B, TCF3B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000125798.14
HGNCiHGNC:5022 FOXA2
MIMi600288 gene
neXtProtiNX_Q9Y261

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3170
OpenTargetsiENSG00000125798
PharmGKBiPA201091

Polymorphism and mutation databases

BioMutaiFOXA2
DMDMi8134491

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000917951 – 457Hepatocyte nuclear factor 3-betaAdd BLAST457

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei156PhosphothreonineBy similarity1
Modified residuei212PhosphoserineBy similarity1
Modified residuei283PhosphoserineBy similarity1
Modified residuei301PhosphothreonineBy similarity1
Modified residuei303PhosphoserineCombined sources1
Modified residuei306PhosphoserineBy similarity1
Modified residuei307PhosphoserineBy similarity1
Modified residuei309PhosphoserineCombined sources1
Modified residuei436PhosphoserineBy similarity1
Modified residuei457PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Y261
PeptideAtlasiQ9Y261
PRIDEiQ9Y261
ProteomicsDBi85664
85665 [Q9Y261-2]

PTM databases

iPTMnetiQ9Y261
PhosphoSitePlusiQ9Y261

Expressioni

Gene expression databases

BgeeiENSG00000125798 Expressed in 72 organ(s), highest expression level in endometrium
CleanExiHS_FOXA2
ExpressionAtlasiQ9Y261 baseline and differential
GenevisibleiQ9Y261 HS

Organism-specific databases

HPAiCAB001388
HPA066846

Interactioni

Subunit structurei

Binds DNA as a monomer. Binds TLE1 (By similarity). Interacts with FOXA1 and FOXA3. Interacts with PRKDC.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109412, 18 interactors
IntActiQ9Y261, 10 interactors
MINTiQ9Y261
STRINGi9606.ENSP00000315955

Structurei

Secondary structure

1457
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y261
SMRiQ9Y261
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 93Transactivation domain 1By similarityAdd BLAST80
Regioni361 – 457Transactivation domain 2By similarityAdd BLAST97

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 113Nuclear localization signalBy similarity8

Phylogenomic databases

eggNOGiKOG3563 Eukaryota
COG5025 LUCA
GeneTreeiENSGT00760000118904
HOGENOMiHOG000231817
HOVERGENiHBG006621
InParanoidiQ9Y261
KOiK08035
OMAiQEHKRGG
PhylomeDBiQ9Y261
TreeFamiTF316127

Family and domain databases

CDDicd00059 FH, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR013638 Fork-head_N
IPR001766 Fork_head_dom
IPR018533 Forkhead_box_C
IPR018122 TF_fork_head_CS_1
IPR030456 TF_fork_head_CS_2
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00250 Forkhead, 1 hit
PF08430 Forkhead_N, 1 hit
PF09354 HNF_C, 1 hit
PRINTSiPR00053 FORKHEAD
SMARTiView protein in SMART
SM00339 FH, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00657 FORK_HEAD_1, 1 hit
PS00658 FORK_HEAD_2, 1 hit
PS50039 FORK_HEAD_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9Y261-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLGAVKMEGH EPSDWSSYYA EPEGYSSVSN MNAGLGMNGM NTYMSMSAAA
60 70 80 90 100
MGSGSGNMSA GSMNMSSYVG AGMSPSLAGM SPGAGAMAGM GGSAGAAGVA
110 120 130 140 150
GMGPHLSPSL SPLGGQAAGA MGGLAPYANM NSMSPMYGQA GLSRARDPKT
160 170 180 190 200
YRRSYTHAKP PYSYISLITM AIQQSPNKML TLSEIYQWIM DLFPFYRQNQ
210 220 230 240 250
QRWQNSIRHS LSFNDCFLKV PRSPDKPGKG SFWTLHPDSG NMFENGCYLR
260 270 280 290 300
RQKRFKCEKQ LALKEAAGAA GSGKKAAAGA QASQAQLGEA AGPASETPAG
310 320 330 340 350
TESPHSSASP CQEHKRGGLG ELKGTPAAAL SPPEPAPSPG QQQQAAAHLL
360 370 380 390 400
GPPHHPGLPP EAHLKPEHHY AFNHPFSINN LMSSEQQHHH SHHHHQPHKM
410 420 430 440 450
DLKAYEQVMH YPGYGSPMPG SLAMGPVTNK TGLDASPLAA DTSYYQGVYS

RPIMNSS
Length:457
Mass (Da):48,306
Last modified:November 1, 1999 - v1
Checksum:i61DDE4C75C70680A
GO
Isoform 2 (identifier: Q9Y261-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MHSASSM

Show »
Length:463
Mass (Da):48,907
Checksum:i726495A18CAE1677
GO

Sequence cautioni

The sequence AAH11780 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_008858328A → V in Japanese subjects with maturity-onset diabetes of the young; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs199796119Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0412121M → MHSASSM in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028021 mRNA Translation: BAA78106.1
AF147787 Genomic DNA Translation: AAD41081.1
AF176110 Genomic DNA Translation: AAD51978.1
AL121722 Genomic DNA No translation available.
BC006545 mRNA Translation: AAH06545.2
BC011780 mRNA Translation: AAH11780.1 Different initiation.
BC019288 mRNA Translation: AAH19288.1
CCDSiCCDS13147.1 [Q9Y261-1]
CCDS46585.1 [Q9Y261-2]
RefSeqiNP_068556.2, NM_021784.4 [Q9Y261-2]
NP_710141.1, NM_153675.2 [Q9Y261-1]
UniGeneiHs.155651

Genome annotation databases

EnsembliENST00000377115; ENSP00000366319; ENSG00000125798 [Q9Y261-1]
ENST00000419308; ENSP00000400341; ENSG00000125798 [Q9Y261-2]
GeneIDi3170
KEGGihsa:3170
UCSCiuc002wsm.4 human [Q9Y261-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Hepatocyte nuclear factors entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028021 mRNA Translation: BAA78106.1
AF147787 Genomic DNA Translation: AAD41081.1
AF176110 Genomic DNA Translation: AAD51978.1
AL121722 Genomic DNA No translation available.
BC006545 mRNA Translation: AAH06545.2
BC011780 mRNA Translation: AAH11780.1 Different initiation.
BC019288 mRNA Translation: AAH19288.1
CCDSiCCDS13147.1 [Q9Y261-1]
CCDS46585.1 [Q9Y261-2]
RefSeqiNP_068556.2, NM_021784.4 [Q9Y261-2]
NP_710141.1, NM_153675.2 [Q9Y261-1]
UniGeneiHs.155651

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5X07X-ray2.80C/F/I/L157-258[»]
ProteinModelPortaliQ9Y261
SMRiQ9Y261
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109412, 18 interactors
IntActiQ9Y261, 10 interactors
MINTiQ9Y261
STRINGi9606.ENSP00000315955

PTM databases

iPTMnetiQ9Y261
PhosphoSitePlusiQ9Y261

Polymorphism and mutation databases

BioMutaiFOXA2
DMDMi8134491

Proteomic databases

PaxDbiQ9Y261
PeptideAtlasiQ9Y261
PRIDEiQ9Y261
ProteomicsDBi85664
85665 [Q9Y261-2]

Protocols and materials databases

DNASUi3170
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377115; ENSP00000366319; ENSG00000125798 [Q9Y261-1]
ENST00000419308; ENSP00000400341; ENSG00000125798 [Q9Y261-2]
GeneIDi3170
KEGGihsa:3170
UCSCiuc002wsm.4 human [Q9Y261-1]

Organism-specific databases

CTDi3170
DisGeNETi3170
EuPathDBiHostDB:ENSG00000125798.14
GeneCardsiFOXA2
HGNCiHGNC:5022 FOXA2
HPAiCAB001388
HPA066846
MIMi600288 gene
neXtProtiNX_Q9Y261
OpenTargetsiENSG00000125798
PharmGKBiPA201091
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3563 Eukaryota
COG5025 LUCA
GeneTreeiENSGT00760000118904
HOGENOMiHOG000231817
HOVERGENiHBG006621
InParanoidiQ9Y261
KOiK08035
OMAiQEHKRGG
PhylomeDBiQ9Y261
TreeFamiTF316127

Enzyme and pathway databases

ReactomeiR-HSA-210745 Regulation of gene expression in beta cells
SignaLinkiQ9Y261
SIGNORiQ9Y261

Miscellaneous databases

ChiTaRSiFOXA2 human
GeneWikiiFOXA2
GenomeRNAii3170
PROiPR:Q9Y261
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000125798 Expressed in 72 organ(s), highest expression level in endometrium
CleanExiHS_FOXA2
ExpressionAtlasiQ9Y261 baseline and differential
GenevisibleiQ9Y261 HS

Family and domain databases

CDDicd00059 FH, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR013638 Fork-head_N
IPR001766 Fork_head_dom
IPR018533 Forkhead_box_C
IPR018122 TF_fork_head_CS_1
IPR030456 TF_fork_head_CS_2
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00250 Forkhead, 1 hit
PF08430 Forkhead_N, 1 hit
PF09354 HNF_C, 1 hit
PRINTSiPR00053 FORKHEAD
SMARTiView protein in SMART
SM00339 FH, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00657 FORK_HEAD_1, 1 hit
PS00658 FORK_HEAD_2, 1 hit
PS50039 FORK_HEAD_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFOXA2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y261
Secondary accession number(s): Q8WUW4, Q96DF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: September 12, 2018
This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again