Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase RNF6

Gene

RNF6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.1 Publication

Miscellaneous

Required for prostate tumor cell growth.

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri632 – 673RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • DNA binding Source: Ensembl
  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF6 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase RNF6Curated
Gene namesi
Name:RNF6
Synonyms:SPG2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000127870.16
HGNCiHGNC:10069 RNF6
MIMi604242 gene
neXtProtiNX_Q9Y252

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Esophageal cancer (ESCR)1 Publication
The disease may be caused by mutations affecting the gene represented in this entry.
Disease descriptionA malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage.
See also OMIM:133239

Organism-specific databases

DisGeNETi6049
MalaCardsiRNF6
MIMi133239 phenotype
OpenTargetsiENSG00000127870
PharmGKBiPA34443

Polymorphism and mutation databases

BioMutaiRNF6
DMDMi13124536

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560471 – 685E3 ubiquitin-protein ligase RNF6Add BLAST685

Proteomic databases

EPDiQ9Y252
PaxDbiQ9Y252
PeptideAtlasiQ9Y252
PRIDEiQ9Y252
ProteomicsDBi85654

PTM databases

iPTMnetiQ9Y252
PhosphoSitePlusiQ9Y252

Expressioni

Tissue specificityi

Weakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to PubMed:18368307, it is preferentially expressed in testis and ovary and hardly detected in other tissues.2 Publications

Developmental stagei

Expressed in embryo and adult testis.1 Publication

Gene expression databases

BgeeiENSG00000127870 Expressed in 235 organ(s), highest expression level in caudate nucleus
CleanExiHS_RNF6
ExpressionAtlasiQ9Y252 baseline and differential
GenevisibleiQ9Y252 HS

Organism-specific databases

HPAiHPA039343
HPA040048

Interactioni

Subunit structurei

Interacts with LIMK1 (By similarity). Interacts with AR.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ARP1027510EBI-2341483,EBI-608057

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111976, 10 interactors
IntActiQ9Y252, 4 interactors
STRINGi9606.ENSP00000342121

Structurei

3D structure databases

ProteinModelPortaliQ9Y252
SMRiQ9Y252
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni607 – 685Required for polyubiquitinationBy similarityAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi292 – 424Arg-richAdd BLAST133
Compositional biasi598 – 601Poly-Asp4

Sequence similaritiesi

Belongs to the RNF12 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri632 – 673RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0800 Eukaryota
ENOG41121N2 LUCA
GeneTreeiENSGT00730000110459
HOGENOMiHOG000273881
HOVERGENiHBG009886
InParanoidiQ9Y252
KOiK22753
OMAiHVWENGA
OrthoDBiEOG091G04S3
PhylomeDBiQ9Y252
TreeFamiTF325756

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF13639 zf-RING_2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9Y252-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNQSRSRSDG GSEETLPQDH NHHENERRWQ QERLHREEAY YQFINELNDE
60 70 80 90 100
DYRLMRDHNL LGTPGEITSE ELQQRLDGVK EQLASQPDLR DGTNYRDSEV
110 120 130 140 150
PRESSHEDSL LEWLNTFRRT GNATRSGQNG NQTWRAVSRT NPNNGEFRFS
160 170 180 190 200
LEIHVNHENR GFEIHGEDYT DIPLSDSNRD HTANRQQRST SPVARRTRSQ
210 220 230 240 250
TSVNFNGSSS NIPRTRLASR GQNPAEGSFS TLGRLRNGIG GAAGIPRANA
260 270 280 290 300
SRTNFSSHTN QSGGSELRQR EGQRFGAAHV WENGARSNVT VRNTNQRLEP
310 320 330 340 350
IRLRSTSNSR SRSPIQRQSG TVYHNSQRES RPVQQTTRRS VRRRGRTRVF
360 370 380 390 400
LEQDRERERR GTAYTPFSNS RLVSRITVEE GEESSRSSTA VRRHPTITLD
410 420 430 440 450
LQVRRIRPGE NRDRDSIANR TRSRVGLAEN TVTIESNSGG FRRTISRLER
460 470 480 490 500
SGIRTYVSTI TVPLRRISEN ELVEPSSVAL RSILRQIMTG FGELSSLMEA
510 520 530 540 550
DSESELQRNG QHLPDMHSEL SNLGTDNNRS QHREGSSQDR QAQGDSTEMH
560 570 580 590 600
GENETTQPHT RNSDSRGGRQ LRNPNNLVET GTLPILRLAH FFLLNESDDD
610 620 630 640 650
DRIRGLTKEQ IDNLSTRHYE HNSIDSELGK ICSVCISDYV TGNKLRQLPC
660 670 680
MHEFHIHCID RWLSENCTCP ICRQPVLGSN IANNG
Length:685
Mass (Da):78,091
Last modified:November 1, 1999 - v1
Checksum:i344584773F2E5EFC
GO
Isoform 2 (identifier: Q9Y252-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MNQSRSRSDGGSEET → MQLEVDKMGTKLGEL
     16-371: Missing.

Note: No experimental confirmation available.
Show »
Length:329
Mass (Da):37,094
Checksum:i03CCF8BF21E456F6
GO
Isoform 3 (identifier: Q9Y252-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-142: VSRTNP → ALPDTS
     143-685: Missing.

Show »
Length:142
Mass (Da):16,680
Checksum:i6ED5C036833DD1DD
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03446548N → S1 PublicationCorresponds to variant dbSNP:rs3910433Ensembl.1
Natural variantiVAR_063490102R → K Found in an esophageal cancer sample; esophageal squamous cell carcinoma; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs121434522EnsemblClinVar.1
Natural variantiVAR_063491164I → T1 PublicationCorresponds to variant dbSNP:rs61760897Ensembl.1
Natural variantiVAR_052094203V → E. Corresponds to variant dbSNP:rs7990167Ensembl.1
Natural variantiVAR_063492242A → T Found in an esophageal cancer sample; esophageal squamous cell carcinoma; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs121434523EnsemblClinVar.1
Natural variantiVAR_063493244G → D Found in an esophageal cancer sample; esophageal squamous cell carcinoma; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs121434524EnsemblClinVar.1
Natural variantiVAR_063494572R → Q1 PublicationCorresponds to variant dbSNP:rs138379662Ensembl.1
Natural variantiVAR_052095623S → N1 PublicationCorresponds to variant dbSNP:rs17083436Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0554241 – 15MNQSR…GSEET → MQLEVDKMGTKLGEL in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_05542516 – 371Missing in isoform 2. 1 PublicationAdd BLAST356
Alternative sequenceiVSP_055426137 – 142VSRTNP → ALPDTS in isoform 3. 1 Publication6
Alternative sequenceiVSP_055427143 – 685Missing in isoform 3. 1 PublicationAdd BLAST543

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010347 mRNA Translation: CAB40414.1
AJ010346 mRNA Translation: CAB40413.1
AF293342 mRNA Translation: AAK00848.1
AY009109 mRNA Translation: AAG49400.1
AK293272 mRNA Translation: BAG56801.1
AK312435 mRNA Translation: BAG35344.1
AL138966 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08380.1
BC034688 mRNA Translation: AAH34688.1
AL133621 mRNA Translation: CAB63747.1
CCDSiCCDS9316.1 [Q9Y252-1]
PIRiT43459
RefSeqiNP_005968.1, NM_005977.3 [Q9Y252-1]
NP_898864.1, NM_183043.2 [Q9Y252-1]
NP_898865.1, NM_183044.2 [Q9Y252-1]
NP_898866.1, NM_183045.1 [Q9Y252-3]
XP_005266542.1, XM_005266485.2 [Q9Y252-1]
XP_005266543.1, XM_005266486.2 [Q9Y252-1]
XP_011533479.1, XM_011535177.2 [Q9Y252-1]
XP_011533480.1, XM_011535178.1 [Q9Y252-3]
UniGeneiHs.136885

Genome annotation databases

EnsembliENST00000346166; ENSP00000342121; ENSG00000127870 [Q9Y252-1]
ENST00000381570; ENSP00000370982; ENSG00000127870 [Q9Y252-1]
ENST00000381588; ENSP00000371000; ENSG00000127870 [Q9Y252-1]
GeneIDi6049
KEGGihsa:6049
UCSCiuc001uqo.4 human [Q9Y252-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010347 mRNA Translation: CAB40414.1
AJ010346 mRNA Translation: CAB40413.1
AF293342 mRNA Translation: AAK00848.1
AY009109 mRNA Translation: AAG49400.1
AK293272 mRNA Translation: BAG56801.1
AK312435 mRNA Translation: BAG35344.1
AL138966 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08380.1
BC034688 mRNA Translation: AAH34688.1
AL133621 mRNA Translation: CAB63747.1
CCDSiCCDS9316.1 [Q9Y252-1]
PIRiT43459
RefSeqiNP_005968.1, NM_005977.3 [Q9Y252-1]
NP_898864.1, NM_183043.2 [Q9Y252-1]
NP_898865.1, NM_183044.2 [Q9Y252-1]
NP_898866.1, NM_183045.1 [Q9Y252-3]
XP_005266542.1, XM_005266485.2 [Q9Y252-1]
XP_005266543.1, XM_005266486.2 [Q9Y252-1]
XP_011533479.1, XM_011535177.2 [Q9Y252-1]
XP_011533480.1, XM_011535178.1 [Q9Y252-3]
UniGeneiHs.136885

3D structure databases

ProteinModelPortaliQ9Y252
SMRiQ9Y252
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111976, 10 interactors
IntActiQ9Y252, 4 interactors
STRINGi9606.ENSP00000342121

PTM databases

iPTMnetiQ9Y252
PhosphoSitePlusiQ9Y252

Polymorphism and mutation databases

BioMutaiRNF6
DMDMi13124536

Proteomic databases

EPDiQ9Y252
PaxDbiQ9Y252
PeptideAtlasiQ9Y252
PRIDEiQ9Y252
ProteomicsDBi85654

Protocols and materials databases

DNASUi6049
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000346166; ENSP00000342121; ENSG00000127870 [Q9Y252-1]
ENST00000381570; ENSP00000370982; ENSG00000127870 [Q9Y252-1]
ENST00000381588; ENSP00000371000; ENSG00000127870 [Q9Y252-1]
GeneIDi6049
KEGGihsa:6049
UCSCiuc001uqo.4 human [Q9Y252-1]

Organism-specific databases

CTDi6049
DisGeNETi6049
EuPathDBiHostDB:ENSG00000127870.16
GeneCardsiRNF6
HGNCiHGNC:10069 RNF6
HPAiHPA039343
HPA040048
MalaCardsiRNF6
MIMi133239 phenotype
604242 gene
neXtProtiNX_Q9Y252
OpenTargetsiENSG00000127870
PharmGKBiPA34443
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0800 Eukaryota
ENOG41121N2 LUCA
GeneTreeiENSGT00730000110459
HOGENOMiHOG000273881
HOVERGENiHBG009886
InParanoidiQ9Y252
KOiK22753
OMAiHVWENGA
OrthoDBiEOG091G04S3
PhylomeDBiQ9Y252
TreeFamiTF325756

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiRNF6 human
GenomeRNAii6049
PROiPR:Q9Y252
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000127870 Expressed in 235 organ(s), highest expression level in caudate nucleus
CleanExiHS_RNF6
ExpressionAtlasiQ9Y252 baseline and differential
GenevisibleiQ9Y252 HS

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF13639 zf-RING_2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNF6_HUMAN
AccessioniPrimary (citable) accession number: Q9Y252
Secondary accession number(s): B4DDP0
, Q5W0H0, Q9BZP5, Q9UF41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: September 12, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again