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Protein

RuvB-like 2

Gene

RUVBL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.
Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84ATP8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Chromatin regulator, Helicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair, Growth regulation, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-171319 Telomere Extension By Telomerase
R-HSA-3214847 HATs acetylate histones

Names & Taxonomyi

Protein namesi
Recommended name:
RuvB-like 2 (EC:3.6.4.12)
Alternative name(s):
48 kDa TATA box-binding protein-interacting protein
Short name:
48 kDa TBP-interacting protein
51 kDa erythrocyte cytosolic protein
Short name:
ECP-51
INO80 complex subunit J
Repressing pontin 52
Short name:
Reptin 52
TIP49b
TIP60-associated protein 54-beta
Short name:
TAP54-beta
Gene namesi
Name:RUVBL2
Synonyms:INO80J, TIP48, TIP49B
ORF Names:CGI-46
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000183207.12
HGNCiHGNC:10475 RUVBL2
MIMi604788 gene
neXtProtiNX_Q9Y230

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi299D → N: Abolishes ATPase activity. 1 Publication1

Organism-specific databases

DisGeNETi10856
OpenTargetsiENSG00000183207
PharmGKBiPA34888

Chemistry databases

ChEMBLiCHEMBL2062349

Polymorphism and mutation databases

BioMutaiRUVBL2
DMDMi28201890

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001656442 – 463RuvB-like 2Add BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Cross-linki9Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei437PhosphoserineCombined sources1
Cross-linki444Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki456Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Y230
PaxDbiQ9Y230
PeptideAtlasiQ9Y230
PRIDEiQ9Y230
ProteomicsDBi85619

2D gel databases

REPRODUCTION-2DPAGEiIPI00009104

PTM databases

iPTMnetiQ9Y230
PhosphoSitePlusiQ9Y230
SwissPalmiQ9Y230

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in testis and thymus.

Gene expression databases

BgeeiENSG00000183207 Expressed in 225 organ(s), highest expression level in testis
CleanExiHS_RUVBL2
ExpressionAtlasiQ9Y230 baseline and differential
GenevisibleiQ9Y230 HS

Organism-specific databases

HPAiCAB012432
HPA042880
HPA067966

Interactioni

Subunit structurei

Forms homohexameric rings (Probable). Can form a dodecamer with RUVBL1 made of two stacked hexameric rings; however, even though RUVBL1 and RUVBL2 are present in equimolar ratio, the oligomeric status of each hexamer is not known. Oligomerization may regulate binding to nucleic acids and conversely, binding to nucleic acids may affect the dodecameric assembly. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex; specifically part of a complex module associated with the helicase ATP-binding and the helicase C-terminal domain of INO80. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with IGHMBP2. Interacts with TELO2. Interacts with HINT1. Component of a SWR1-like complex. Component of the R2TP complex composed at least of PIHD1, RUVBL1, RUVBL2 and RPAP3 (PubMed:20864032). Interacts with ITFG1 (PubMed:25437307). Interacts with ZMYND10 (PubMed:29601588). Interacts with WAC; WAC positively regulates MTOR activity by promoting the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex which leads to the dimerization of the mTORC1 complex and its subsequent activation (PubMed:26812014).Curated20 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116067, 281 interactors
ComplexPortaliCPX-846 INO80 chromatin remodeling complex
CPX-974 SRCAP histone exchanging complex
CPX-978 NuA4 histone acetyltransferase complex
CORUMiQ9Y230
DIPiDIP-28153N
IntActiQ9Y230, 127 interactors
MINTiQ9Y230
STRINGi9606.ENSP00000473172

Structurei

Secondary structure

1463
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y230
SMRiQ9Y230
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y230

Family & Domainsi

Domaini

The C-terminal domain is required for association with ATF2.

Sequence similaritiesi

Belongs to the RuvB family.Curated

Phylogenomic databases

eggNOGiKOG2680 Eukaryota
COG1224 LUCA
GeneTreeiENSGT00890000139527
HOGENOMiHOG000190885
HOVERGENiHBG054186
InParanoidiQ9Y230
KOiK11338
OMAiVPFTMIA
OrthoDBiEOG091G07C9
PhylomeDBiQ9Y230
TreeFamiTF300469

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR027417 P-loop_NTPase
IPR027238 RuvB-like
IPR037942 RUVBL2
IPR010339 TIP49_C
PANTHERiPTHR11093 PTHR11093, 1 hit
PTHR11093:SF2 PTHR11093:SF2, 1 hit
PfamiView protein in Pfam
PF06068 TIP49, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y230-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATVTATTKV PEIRDVTRIE RIGAHSHIRG LGLDDALEPR QASQGMVGQL
60 70 80 90 100
AARRAAGVVL EMIREGKIAG RAVLIAGQPG TGKTAIAMGM AQALGPDTPF
110 120 130 140 150
TAIAGSEIFS LEMSKTEALT QAFRRSIGVR IKEETEIIEG EVVEIQIDRP
160 170 180 190 200
ATGTGSKVGK LTLKTTEMET IYDLGTKMIE SLTKDKVQAG DVITIDKATG
210 220 230 240 250
KISKLGRSFT RARDYDAMGS QTKFVQCPDG ELQKRKEVVH TVSLHEIDVI
260 270 280 290 300
NSRTQGFLAL FSGDTGEIKS EVREQINAKV AEWREEGKAE IIPGVLFIDE
310 320 330 340 350
VHMLDIESFS FLNRALESDM APVLIMATNR GITRIRGTSY QSPHGIPIDL
360 370 380 390 400
LDRLLIVSTT PYSEKDTKQI LRIRCEEEDV EMSEDAYTVL TRIGLETSLR
410 420 430 440 450
YAIQLITAAS LVCRKRKGTE VQVDDIKRVY SLFLDESRST QYMKEYQDAF
460
LFNELKGETM DTS
Length:463
Mass (Da):51,157
Last modified:January 23, 2007 - v3
Checksum:i54C78E9C587D975A
GO
Isoform 2 (identifier: Q9Y230-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Note: No experimental confirmation available.
Show »
Length:418
Mass (Da):46,306
Checksum:i64C304B804D86B2E
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0R0Y3M0R0Y3_HUMAN
RuvB-like helicase
RUVBL2
354Annotation score:
M0QXI6M0QXI6_HUMAN
RuvB-like helicase
RUVBL2
109Annotation score:
X6R2L4X6R2L4_HUMAN
RuvB-like helicase
RUVBL2
259Annotation score:
M0R0Z0M0R0Z0_HUMAN
RuvB-like helicase
RUVBL2
50Annotation score:
A0A1W2PS48A0A1W2PS48_HUMAN
RuvB-like helicase
RUVBL2
50Annotation score:
M0QXZ7M0QXZ7_HUMAN
RuvB-like 2 (E. coli), isoform CRA_...
RUVBL2 hCG_16214
39Annotation score:
M0QYD8M0QYD8_HUMAN
RuvB-like 2 (E. coli), isoform CRA_...
RUVBL2 hCG_16214
44Annotation score:
A0A1W2PS22A0A1W2PS22_HUMAN
RuvB-like 2
RUVBL2
39Annotation score:

Sequence cautioni

The sequence AAD34041 differs from that shown. Reason: Frameshift at position 401.Curated
The sequence AAH08355 differs from that shown. Reason: Frameshift at position 191.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti214D → N in AAD34041 (PubMed:10810093).Curated1
Sequence conflicti257 – 258FL → YV AA sequence (PubMed:10882073).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0565841 – 45Missing in isoform 2. 1 PublicationAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18417 mRNA Translation: CAB46270.1
AB024301 mRNA Translation: BAA76708.1
AF155138 mRNA Translation: AAD38073.1
AF124607 mRNA Translation: AAF87087.1
AF151804 mRNA Translation: AAD34041.1 Frameshift.
AL136743 mRNA Translation: CAB66677.1
AK057498 mRNA Translation: BAG51921.1
AK074542 mRNA Translation: BAC11048.1
CR533507 mRNA Translation: CAG38538.1
AC008687 Genomic DNA No translation available.
CH471177 Genomic DNA Translation: EAW52426.1
CH471177 Genomic DNA Translation: EAW52430.1
BC000428 mRNA Translation: AAH00428.1
BC004531 mRNA Translation: AAH04531.1
BC008355 mRNA Translation: AAH08355.1 Frameshift.
CCDSiCCDS42588.1 [Q9Y230-1]
PIRiT46313
RefSeqiNP_001308120.1, NM_001321191.1 [Q9Y230-2]
NP_006657.1, NM_006666.2 [Q9Y230-1]
XP_011524632.1, XM_011526330.1 [Q9Y230-2]
UniGeneiHs.515846

Genome annotation databases

EnsembliENST00000595090; ENSP00000473172; ENSG00000183207 [Q9Y230-1]
GeneIDi10856
KEGGihsa:10856
UCSCiuc002plr.2 human [Q9Y230-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18417 mRNA Translation: CAB46270.1
AB024301 mRNA Translation: BAA76708.1
AF155138 mRNA Translation: AAD38073.1
AF124607 mRNA Translation: AAF87087.1
AF151804 mRNA Translation: AAD34041.1 Frameshift.
AL136743 mRNA Translation: CAB66677.1
AK057498 mRNA Translation: BAG51921.1
AK074542 mRNA Translation: BAC11048.1
CR533507 mRNA Translation: CAG38538.1
AC008687 Genomic DNA No translation available.
CH471177 Genomic DNA Translation: EAW52426.1
CH471177 Genomic DNA Translation: EAW52430.1
BC000428 mRNA Translation: AAH00428.1
BC004531 mRNA Translation: AAH04531.1
BC008355 mRNA Translation: AAH08355.1 Frameshift.
CCDSiCCDS42588.1 [Q9Y230-1]
PIRiT46313
RefSeqiNP_001308120.1, NM_001321191.1 [Q9Y230-2]
NP_006657.1, NM_006666.2 [Q9Y230-1]
XP_011524632.1, XM_011526330.1 [Q9Y230-2]
UniGeneiHs.515846

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CQANMR-A132-213[»]
2XSZX-ray3.00D/E/F2-133[»]
D/E/F238-463[»]
3UK6X-ray2.95A/B/C/D/E/F/G/H/I/J/K/L1-132[»]
A/B/C/D/E/F/G/H/I/J/K/L239-463[»]
5OAFelectron microscopy4.06B/D/F1-463[»]
6ETXelectron microscopy4.80B/D/F1-463[»]
6FO1electron microscopy3.57D/E/F1-463[»]
6H7XX-ray2.89A1-463[»]
ProteinModelPortaliQ9Y230
SMRiQ9Y230
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116067, 281 interactors
ComplexPortaliCPX-846 INO80 chromatin remodeling complex
CPX-974 SRCAP histone exchanging complex
CPX-978 NuA4 histone acetyltransferase complex
CORUMiQ9Y230
DIPiDIP-28153N
IntActiQ9Y230, 127 interactors
MINTiQ9Y230
STRINGi9606.ENSP00000473172

Chemistry databases

ChEMBLiCHEMBL2062349

PTM databases

iPTMnetiQ9Y230
PhosphoSitePlusiQ9Y230
SwissPalmiQ9Y230

Polymorphism and mutation databases

BioMutaiRUVBL2
DMDMi28201890

2D gel databases

REPRODUCTION-2DPAGEiIPI00009104

Proteomic databases

EPDiQ9Y230
PaxDbiQ9Y230
PeptideAtlasiQ9Y230
PRIDEiQ9Y230
ProteomicsDBi85619

Protocols and materials databases

DNASUi10856
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000595090; ENSP00000473172; ENSG00000183207 [Q9Y230-1]
GeneIDi10856
KEGGihsa:10856
UCSCiuc002plr.2 human [Q9Y230-1]

Organism-specific databases

CTDi10856
DisGeNETi10856
EuPathDBiHostDB:ENSG00000183207.12
GeneCardsiRUVBL2
HGNCiHGNC:10475 RUVBL2
HPAiCAB012432
HPA042880
HPA067966
MIMi604788 gene
neXtProtiNX_Q9Y230
OpenTargetsiENSG00000183207
PharmGKBiPA34888
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2680 Eukaryota
COG1224 LUCA
GeneTreeiENSGT00890000139527
HOGENOMiHOG000190885
HOVERGENiHBG054186
InParanoidiQ9Y230
KOiK11338
OMAiVPFTMIA
OrthoDBiEOG091G07C9
PhylomeDBiQ9Y230
TreeFamiTF300469

Enzyme and pathway databases

ReactomeiR-HSA-171319 Telomere Extension By Telomerase
R-HSA-3214847 HATs acetylate histones

Miscellaneous databases

ChiTaRSiRUVBL2 human
EvolutionaryTraceiQ9Y230
GeneWikiiRUVBL2
GenomeRNAii10856
PROiPR:Q9Y230
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000183207 Expressed in 225 organ(s), highest expression level in testis
CleanExiHS_RUVBL2
ExpressionAtlasiQ9Y230 baseline and differential
GenevisibleiQ9Y230 HS

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR027417 P-loop_NTPase
IPR027238 RuvB-like
IPR037942 RUVBL2
IPR010339 TIP49_C
PANTHERiPTHR11093 PTHR11093, 1 hit
PTHR11093:SF2 PTHR11093:SF2, 1 hit
PfamiView protein in Pfam
PF06068 TIP49, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRUVB2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y230
Secondary accession number(s): B3KQ59
, E7ETE5, Q6FIB9, Q6PK27, Q9Y361
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 211 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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