Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ectonucleoside triphosphate diphosphohydrolase 4

Gene

ENTPD4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes preferentially nucleoside 5'-diphosphates, nucleoside 5'-triphosphates are hydrolyzed only to a minor extent. The order of activity with different substrates is UDP >> GDP = CDP = TDP, AMP, ADP, ATP and UMP are not substrates. Preferred substrates for isoform 2 are CTP, UDP, CDP, GTP and GDP, while isoform 1 utilizes UTP and TTP.

Catalytic activityi

A nucleoside diphosphate + H2O = a nucleoside phosphate + phosphate.1 Publication

Cofactori

Ca2+1 Publication, Mg2+1 PublicationNote: Isoform 1 and isoform 2 require Ca2+ and can also use Mg2+, but with lower efficiency.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei222Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandCalcium, Magnesium

Enzyme and pathway databases

BioCyciMetaCyc:HS09501-MONOMER
ReactomeiR-HSA-8850843 Phosphate bond hydrolysis by NTPDase proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 4 (EC:3.6.1.6)
Short name:
NTPDase 4
Alternative name(s):
Lysosomal apyrase-like protein of 70 kDa
Uridine-diphosphatase
Short name:
UDPase
Gene namesi
Name:ENTPD4
Synonyms:KIAA0392, LALP70, LYSAL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000197217.12
HGNCiHGNC:14573 ENTPD4
MIMi607577 gene
neXtProtiNX_Q9Y227

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 559LumenalSequence analysisAdd BLAST505
Transmembranei560 – 580HelicalSequence analysisAdd BLAST21
Topological domaini581 – 616CytoplasmicSequence analysisAdd BLAST36

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9583
OpenTargetsiENSG00000197217
PharmGKBiPA30502

Chemistry databases

ChEMBLiCHEMBL3313834

Polymorphism and mutation databases

BioMutaiENTPD4
DMDMi18203627

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002099111 – 616Ectonucleoside triphosphate diphosphohydrolase 4Add BLAST616

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi404N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi407N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi461 ↔ 490By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ9Y227
PaxDbiQ9Y227
PeptideAtlasiQ9Y227
PRIDEiQ9Y227
ProteomicsDBi85614
85615 [Q9Y227-2]

PTM databases

iPTMnetiQ9Y227
PhosphoSitePlusiQ9Y227
SwissPalmiQ9Y227

Expressioni

Tissue specificityi

Ubiquitous. Highest expression in testis and lowest in bladder.

Gene expression databases

BgeeiENSG00000197217 Expressed in 228 organ(s), highest expression level in small intestine Peyer's patch
CleanExiHS_ENTPD4
ExpressionAtlasiQ9Y227 baseline and differential
GenevisibleiQ9Y227 HS

Organism-specific databases

HPAiHPA017655

Interactioni

Protein-protein interaction databases

BioGridi114951, 10 interactors
IntActiQ9Y227, 2 interactors
STRINGi9606.ENSP00000351520

Structurei

3D structure databases

ProteinModelPortaliQ9Y227
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1386 Eukaryota
COG5371 LUCA
GeneTreeiENSGT00550000074435
HOGENOMiHOG000090205
HOVERGENiHBG006789
InParanoidiQ9Y227
KOiK12305
OMAiGCDAHRT
OrthoDBiEOG091G04IV
PhylomeDBiQ9Y227
TreeFamiTF354343

Family and domain databases

InterProiView protein in InterPro
IPR000407 GDA1_CD39_NTPase
PANTHERiPTHR11782 PTHR11782, 1 hit
PfamiView protein in Pfam
PF01150 GDA1_CD39, 1 hit
PROSITEiView protein in PROSITE
PS01238 GDA1_CD39_NTPASE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y227-1) [UniParc]FASTAAdd to basket
Also known as: LALP70

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGRIGISCLF PASWHFSISP VGCPRILNTN LRQIMVISVL AAAVSLLYFS
60 70 80 90 100
VVIIRNKYGR LTRDKKFQRY LARVTDIEAT DTNNPNVNYG IVVDCGSSGS
110 120 130 140 150
RVFVYCWPRH NGNPHDLLDI RQMRDKNRKP VVMKIKPGIS EFATSPEKVS
160 170 180 190 200
DYISPLLNFA AEHVPRAKHK ETPLYILCTA GMRILPESQQ KAILEDLLTD
210 220 230 240 250
IPVHFDFLFS DSHAEVISGK QEGVYAWIGI NFVLGRFEHI EDDDEAVVEV
260 270 280 290 300
NIPGSESSEA IVRKRTAGIL DMGGVSTQIA YEVPKTVSFA SSQQEEVAKN
310 320 330 340 350
LLAEFNLGCD VHQTEHVYRV YVATFLGFGG NAARQRYEDR IFANTIQKNR
360 370 380 390 400
LLGKQTGLTP DMPYLDPCLP LDIKDEIQQN GQTIYLRGTG DFDLCRETIQ
410 420 430 440 450
PFMNKTNETQ TSLNGVYQPP IHFQNSEFYG FSEFYYCTED VLRMGGDYNA
460 470 480 490 500
AKFTKAAKDY CATKWSILRE RFDRGLYASH ADLHRLKYQC FKSAWMFEVF
510 520 530 540 550
HRGFSFPVNY KSLKTALQVY DKEVQWTLGA ILYRTRFLPL RDIQQEAFRA
560 570 580 590 600
SHTHWRGVSF VYNHYLFSGC FLVVLLAILL YLLRLRRIHR RTPRSSSAAA
610
LWMEEGLPAQ NAPGTL
Length:616
Mass (Da):70,255
Last modified:November 1, 1999 - v1
Checksum:i68A7EF7E9CB60544
GO
Isoform 2 (identifier: Q9Y227-2) [UniParc]FASTAAdd to basket
Also known as: LALP70V

The sequence of this isoform differs from the canonical sequence as follows:
     287-294: Missing.

Show »
Length:608
Mass (Da):69,421
Checksum:iF931A79B05D600A9
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8NE73Q8NE73_HUMAN
ENTPD4 protein
ENTPD4
571Annotation score:
H0YBY8H0YBY8_HUMAN
Ectonucleoside triphosphate diphosp...
ENTPD4
214Annotation score:
E5RIB2E5RIB2_HUMAN
Ectonucleoside triphosphate diphosp...
ENTPD4
127Annotation score:
H0YAN2H0YAN2_HUMAN
Ectonucleoside triphosphate diphosp...
ENTPD4
117Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43A → AA in AAC17217 (PubMed:9556635).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_064711341I → V Found in a renal cell carcinoma case; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs549468877Ensembl.1
Natural variantiVAR_020444354K → E. Corresponds to variant dbSNP:rs2272641Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003614287 – 294Missing in isoform 2. 2 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016032 mRNA Translation: AAC17217.1
AJ131358 mRNA Translation: CAB40415.1
AJ246165
, AJ246166, AJ246167, AJ246168, AJ246169, AJ246170, AJ246171, AJ246172, AJ246173, AJ246174, AJ246175, AJ246176 Genomic DNA Translation: CAB45655.1
CH471080 Genomic DNA Translation: EAW63622.1
CH471080 Genomic DNA Translation: EAW63623.1
AB002390 mRNA Translation: BAA21575.1
CCDSiCCDS47827.1 [Q9Y227-2]
CCDS6041.1 [Q9Y227-1]
RefSeqiNP_001122402.1, NM_001128930.2 [Q9Y227-2]
NP_004892.1, NM_004901.4 [Q9Y227-1]
UniGeneiHs.444389

Genome annotation databases

EnsembliENST00000358689; ENSP00000351520; ENSG00000197217 [Q9Y227-1]
ENST00000417069; ENSP00000408573; ENSG00000197217 [Q9Y227-2]
GeneIDi9583
KEGGihsa:9583
UCSCiuc003xdl.4 human [Q9Y227-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016032 mRNA Translation: AAC17217.1
AJ131358 mRNA Translation: CAB40415.1
AJ246165
, AJ246166, AJ246167, AJ246168, AJ246169, AJ246170, AJ246171, AJ246172, AJ246173, AJ246174, AJ246175, AJ246176 Genomic DNA Translation: CAB45655.1
CH471080 Genomic DNA Translation: EAW63622.1
CH471080 Genomic DNA Translation: EAW63623.1
AB002390 mRNA Translation: BAA21575.1
CCDSiCCDS47827.1 [Q9Y227-2]
CCDS6041.1 [Q9Y227-1]
RefSeqiNP_001122402.1, NM_001128930.2 [Q9Y227-2]
NP_004892.1, NM_004901.4 [Q9Y227-1]
UniGeneiHs.444389

3D structure databases

ProteinModelPortaliQ9Y227
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114951, 10 interactors
IntActiQ9Y227, 2 interactors
STRINGi9606.ENSP00000351520

Chemistry databases

ChEMBLiCHEMBL3313834

PTM databases

iPTMnetiQ9Y227
PhosphoSitePlusiQ9Y227
SwissPalmiQ9Y227

Polymorphism and mutation databases

BioMutaiENTPD4
DMDMi18203627

Proteomic databases

EPDiQ9Y227
PaxDbiQ9Y227
PeptideAtlasiQ9Y227
PRIDEiQ9Y227
ProteomicsDBi85614
85615 [Q9Y227-2]

Protocols and materials databases

DNASUi9583
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358689; ENSP00000351520; ENSG00000197217 [Q9Y227-1]
ENST00000417069; ENSP00000408573; ENSG00000197217 [Q9Y227-2]
GeneIDi9583
KEGGihsa:9583
UCSCiuc003xdl.4 human [Q9Y227-1]

Organism-specific databases

CTDi9583
DisGeNETi9583
EuPathDBiHostDB:ENSG00000197217.12
GeneCardsiENTPD4
HGNCiHGNC:14573 ENTPD4
HPAiHPA017655
MIMi607577 gene
neXtProtiNX_Q9Y227
OpenTargetsiENSG00000197217
PharmGKBiPA30502
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1386 Eukaryota
COG5371 LUCA
GeneTreeiENSGT00550000074435
HOGENOMiHOG000090205
HOVERGENiHBG006789
InParanoidiQ9Y227
KOiK12305
OMAiGCDAHRT
OrthoDBiEOG091G04IV
PhylomeDBiQ9Y227
TreeFamiTF354343

Enzyme and pathway databases

BioCyciMetaCyc:HS09501-MONOMER
ReactomeiR-HSA-8850843 Phosphate bond hydrolysis by NTPDase proteins

Miscellaneous databases

ChiTaRSiENTPD4 human
GenomeRNAii9583
PROiPR:Q9Y227
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000197217 Expressed in 228 organ(s), highest expression level in small intestine Peyer's patch
CleanExiHS_ENTPD4
ExpressionAtlasiQ9Y227 baseline and differential
GenevisibleiQ9Y227 HS

Family and domain databases

InterProiView protein in InterPro
IPR000407 GDA1_CD39_NTPase
PANTHERiPTHR11782 PTHR11782, 1 hit
PfamiView protein in Pfam
PF01150 GDA1_CD39, 1 hit
PROSITEiView protein in PROSITE
PS01238 GDA1_CD39_NTPASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiENTP4_HUMAN
AccessioniPrimary (citable) accession number: Q9Y227
Secondary accession number(s): D3DSS3, O15092
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again