UniProtKB - Q9Y1I2 (VP33A_DROME)
Vacuolar protein sorting-associated protein 33A
car
Functioni
Required for the biogenesis of eye pigment granules (PubMed:10549280).
Plays a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes probably as part of the class C core vacuole/endosome tethering (CORVET) complex (PubMed:10549280, PubMed:22160599, PubMed:27253064).
1 Publication2 PublicationsGO - Molecular functioni
- SNARE binding Source: FlyBase
- syntaxin binding Source: FlyBase
GO - Biological processi
- autophagosome maturation Source: FlyBase
- cellular response to starvation Source: FlyBase
- compound eye pigmentation Source: FlyBase
- determination of adult lifespan Source: FlyBase
- endocytosis Source: FlyBase
- endosomal transport Source: FlyBase
- endosome organization Source: FlyBase
- endosome to lysosome transport Source: FlyBase
- eye pigment granule organization Source: UniProtKB
- intracellular protein transport Source: GO_Central
- intracellular transport Source: FlyBase
- Notch receptor processing Source: FlyBase
- ommochrome biosynthetic process Source: FlyBase
- protein targeting to lysosome Source: UniProtKB
- regulation of protein ubiquitination Source: FlyBase
- regulation of SNARE complex assembly Source: FlyBase
- vesicle-mediated transport Source: FlyBase
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
SignaLinki | Q9Y1I2 |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 33AAlternative name(s): Protein carnation |
Gene namesi | Name:car ORF Names:CG12230 |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0000257, car |
VEuPathDBi | VectorBase:FBgn0000257 |
Subcellular locationi
Endosome
- Early endosome 1 Publication
- Late endosome membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Lysosome
- Lysosome membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Note: Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
Cytosol
- cytosol Source: FlyBase
Endosome
- CORVET complex Source: FlyBase
- early endosome Source: UniProtKB-SubCell
- late endosome membrane Source: UniProtKB-SubCell
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
- lysosome Source: UniProtKB
Other locations
- HOPS complex Source: FlyBase
- membrane Source: FlyBase
- vacuole Source: GO_Central
Keywords - Cellular componenti
Endosome, Lysosome, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 26 | L → V in car-1; irregular clustering of early endosomes; when associated with V-249. 1 Publication | 1 | |
Mutagenesisi | 249 | G → V in car-1; irregular clustering of early endosomes; when associated with V-26. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206308 | 1 – 617 | Vacuolar protein sorting-associated protein 33AAdd BLAST | 617 |
Proteomic databases
PaxDbi | Q9Y1I2 |
PRIDEi | Q9Y1I2 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0000257, Expressed in head capsule and 53 other tissues |
ExpressionAtlasi | Q9Y1I2, baseline and differential |
Genevisiblei | Q9Y1I2, DM |
Interactioni
Subunit structurei
Component of the class C core vacuole/endosome tethering (CORVET) complex composed of at least dor/Vps18, Vps16A, Vps8 and car/Vps33A; unlike in other species, Vps11 is not part of the Drosophila complex (PubMed:27253064).
Interacts with dor (PubMed:10549280).
Interacts with ema (PubMed:20194640).
3 PublicationsGO - Molecular functioni
- SNARE binding Source: FlyBase
- syntaxin binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 59247, 16 interactors |
IntActi | Q9Y1I2, 1 interactor |
STRINGi | 7227.FBpp0074496 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 268 – 287 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1302, Eukaryota |
GeneTreei | ENSGT00940000155165 |
HOGENOMi | CLU_016678_3_0_1 |
InParanoidi | Q9Y1I2 |
OMAi | QCVLQKP |
OrthoDBi | 406738at2759 |
PhylomeDBi | Q9Y1I2 |
Family and domain databases
Gene3Di | 1.25.40.850, 1 hit 3.40.50.1910, 2 hits 3.40.50.2060, 1 hit |
InterProi | View protein in InterPro IPR043154, Sec-1-like_dom1 IPR001619, Sec1-like IPR027482, Sec1-like_dom2 IPR036045, Sec1-like_sf IPR027121, VPS33 IPR043155, VPS33_dom3b |
PANTHERi | PTHR11679, PTHR11679, 1 hit PTHR11679:SF1, PTHR11679:SF1, 1 hit |
Pfami | View protein in Pfam PF00995, Sec1, 1 hit |
PIRSFi | PIRSF005715, VPS45_Sec1, 1 hit |
SUPFAMi | SSF56815, SSF56815, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MFPHLKGHGQ RVNLQLLQEA ACRELLQQLD RIEGSKVIVL DETMIGPLDL
60 70 80 90 100
VTRPKLFADR GIRLLALKPE LHLPREVANV VYVMRPRVAL MEQLAAHVKA
110 120 130 140 150
GGRAAAGRQY HILFAPRRSC LCVSQLEVSG VLGSFGNIEE LAWNYLPLDV
160 170 180 190 200
DLVSMEMPNA FRDVSVDGDT SSLYQAAVGL VQLQRLYGRI PKIYGKGEFA
210 220 230 240 250
HRVWEHAKQL GRDERTLYNG DKGVVDQLIL LDRSIDLLSP LATQLTYEGL
260 270 280 290 300
IDEFYGIRQN KLTLPAENFP SDGALPGGGG SGPRVEESQS LLGDTEKKTI
310 320 330 340 350
LLHSGEQLYA ELRNKHFNEV TKLLARKARE IHVQMHATSQ DKSVQEIKSF
360 370 380 390 400
VENLLPQLMA QKKATSEHTA IAGLLHEQVN AVRFADDLAA EQEFMVCADI
410 420 430 440 450
DKPSAYIEDL IACRVELNRV LRLICMQCHA ASGFKEKLLN HYKRELVHVY
460 470 480 490 500
GLEVLLTISN LEKSGLLHLQ TESRAYSVLR KTLHLTVDDN VEIEPKDISY
510 520 530 540 550
VHSFYAPLTA RIVEHSLKPL GWQTLKSQIN NLPGPTFEDF QAQLVGIGGR
560 570 580 590 600
HTVTTVSEGS LLNVPRVVLV CFVGGCTFAE IAALRFLAAQ EDNNVEFLIA
610
TTKVVNKHSF LDSLMSS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA4V4S5 | A4V4S5_DROME | Carnation, isoform B | car Car, carn, carnation, Dm-Vps33, Dmel\CG12230 | 617 | Annotation score: | ||
M9PF72 | M9PF72_DROME | Carnation, isoform C | car Car, carn, carnation, Dm-Vps33, Dmel\CG12230 | 639 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF133260 mRNA Translation: AAD38513.1 AE014298 Genomic DNA Translation: AAF48972.1 AY069498 mRNA Translation: AAL39643.1 |
RefSeqi | NP_523410.1, NM_078686.5 NP_728266.1, NM_167664.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0074727; FBpp0074496; FBgn0000257 FBtr0074728; FBpp0074497; FBgn0000257 |
GeneIDi | 32947 |
KEGGi | dme:Dmel_CG12230 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF133260 mRNA Translation: AAD38513.1 AE014298 Genomic DNA Translation: AAF48972.1 AY069498 mRNA Translation: AAL39643.1 |
RefSeqi | NP_523410.1, NM_078686.5 NP_728266.1, NM_167664.2 |
3D structure databases
SMRi | Q9Y1I2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 59247, 16 interactors |
IntActi | Q9Y1I2, 1 interactor |
STRINGi | 7227.FBpp0074496 |
Proteomic databases
PaxDbi | Q9Y1I2 |
PRIDEi | Q9Y1I2 |
Protocols and materials databases
DNASUi | 32947 |
Genome annotation databases
EnsemblMetazoai | FBtr0074727; FBpp0074496; FBgn0000257 FBtr0074728; FBpp0074497; FBgn0000257 |
GeneIDi | 32947 |
KEGGi | dme:Dmel_CG12230 |
Organism-specific databases
CTDi | 32947 |
FlyBasei | FBgn0000257, car |
VEuPathDBi | VectorBase:FBgn0000257 |
Phylogenomic databases
eggNOGi | KOG1302, Eukaryota |
GeneTreei | ENSGT00940000155165 |
HOGENOMi | CLU_016678_3_0_1 |
InParanoidi | Q9Y1I2 |
OMAi | QCVLQKP |
OrthoDBi | 406738at2759 |
PhylomeDBi | Q9Y1I2 |
Enzyme and pathway databases
SignaLinki | Q9Y1I2 |
Miscellaneous databases
BioGRID-ORCSi | 32947, 0 hits in 1 CRISPR screen |
GenomeRNAii | 32947 |
PROi | PR:Q9Y1I2 |
Gene expression databases
Bgeei | FBgn0000257, Expressed in head capsule and 53 other tissues |
ExpressionAtlasi | Q9Y1I2, baseline and differential |
Genevisiblei | Q9Y1I2, DM |
Family and domain databases
Gene3Di | 1.25.40.850, 1 hit 3.40.50.1910, 2 hits 3.40.50.2060, 1 hit |
InterProi | View protein in InterPro IPR043154, Sec-1-like_dom1 IPR001619, Sec1-like IPR027482, Sec1-like_dom2 IPR036045, Sec1-like_sf IPR027121, VPS33 IPR043155, VPS33_dom3b |
PANTHERi | PTHR11679, PTHR11679, 1 hit PTHR11679:SF1, PTHR11679:SF1, 1 hit |
Pfami | View protein in Pfam PF00995, Sec1, 1 hit |
PIRSFi | PIRSF005715, VPS45_Sec1, 1 hit |
SUPFAMi | SSF56815, SSF56815, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VP33A_DROME | |
Accessioni | Q9Y1I2Primary (citable) accession number: Q9Y1I2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families