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Entry version 155 (11 Dec 2019)
Sequence version 1 (01 Nov 1999)
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Protein

DNA polymerase delta subunit 3

Gene

Pol32

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Accessory component of the DNA polymerase delta complex and possibly the DNA polymerase zeta complex (By similarity). As a component of the delta complex, participates in high fidelity genome replication, including lagging strand synthesis, DNA recombination and repair (PubMed:22532806, PubMed:25826374, PubMed:31100062). Required to recruit the DNA polymerase delta complex to the nucleus of rapidly dividing embryonic cells, and as a consequence is essential for genome replication during the earliest cell cycles (PubMed:31100062, PubMed:25826374). Increases the efficiency and processivity of DNA synthesis of the DNA polymerases during mitotic DNA replication and repair (PubMed:22532806, PubMed:25826374, PubMed:31100062). During development this function is essential for preventing replication stress that results in the formation of chromosomal fragile sites (CFS) such as chromosomal breaks (PubMed:31100062, PubMed:25826374). Ensures genomic stability by promoting several types of DNA repair mechanisms including repairing broken dicentric chromosomes through homolog-dependent break-induced replication (BIR) (PubMed:22532806, PubMed:25826374, PubMed:31053594). During homologous recombination (HR) repair, required for maintaining the processivity of the delta complex during break-induced replication; a form of HR that requires extensive DNA synthesis such as the repair of large gaps (PubMed:25826374, PubMed:22532806). Able to suppress position effect variegation and may therefore have a role in the induction of chromatin state changes that likely include its activities in DNA replication and repair (PubMed:25826374).By similarity4 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processDNA damage, DNA repair, DNA replication

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA polymerase delta subunit 3Curated
Alternative name(s):
Pol32 polymerase delta subunit 3By similarity
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Pol32Imported
Synonyms:DNApolD3Curated, rdImported
ORF Names:CG3975Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2L

Organism-specific databases

Drosophila genome database

More...
FlyBasei
FBgn0283467 Pol32

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Oocytes and embryos lacking maternal pol32, show no nuclear localization of the delta complex members DNApol-delta and Pol31, resulting in limited genome replication during the earliest cell cycles and thus early developmental arrest (PubMed:31100062). Mutants, presumably with maternal but not zygotic pol32, develop normally however adults display a variable degree of bristle loss or shortening, and females are sterile while males are fertile (PubMed:31100062). In larval salivary glands, the delta complex localizes normally to the nuclei and chromosomes display a significant decrease in homologous recombination repair (PubMed:31100062). Chromosomes also exhibit spontaneous double strand breaks that primarily result from defects in genomic replication and, to a lesser extent, in DNA repair synthesis (PubMed:31100062).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi15 – 431Missing : Homologous recombination repair is reduced by 70% resulting in mutants displaying extreme sensitivity to various DNA damaging agents, except for camptothecin. 1 PublicationAdd BLAST417
Mutagenesisi55 – 88Missing : Unable to rescue bristle and female fertility defects in mutants. 1 PublicationAdd BLAST34
Mutagenesisi64 – 80Missing : Unable to rescue bristle and female fertility defects in mutants. 1 PublicationAdd BLAST17
Mutagenesisi371 – 378Missing : Rescues bristle and female fertility defects in mutants. 1 Publication8
Mutagenesisi371 – 373DED → AAA: Rescues bristle and female fertility defects in mutants. 1 Publication3
Mutagenesisi374 – 378GFVIT → AFVAA: Rescues bristle and female fertility defects in mutants. 1 Publication5
Mutagenesisi421 – 428Missing : Rescues bristle and female fertility defects in mutants. 1 Publication8
Mutagenesisi421 – 424QAGI → AAGA: Rescues bristle and female fertility defects in mutants. 1 Publication4

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004486871 – 431DNA polymerase delta subunit 3Add BLAST431

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9Y118

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in ovaries (at the protein level) (PubMed:31100062). Expressed in ovaries (PubMed:25826374).2 Publications

<p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expressed in adult females and embryos (at the protein level).1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
FBgn0027559 Expressed in 3 organ(s), highest expression level in larva

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of both the DNA polymerase delta and DNA polymerase zeta complexes (By similarity). The DNA polymerase delta complex consists of three subunits: the catalytic subunit DNApol-delta/DNApolD1 and two accessory subunits Pol31/DNApolD2 and Pol32/DNApolD3 (PubMed:31100062). Within the delta complex, interacts with both DNApol-delta and Pol31 (PubMed:31100062).

Component of the DNA polymerase zeta complex consisting of four subunits: the catalytic subunit DNApolZ1 and three accessory subunits Rev7/DNApolZ2, Pol31/DNApolD2 and Pol32/DNApolD3 (By similarity).

By similarity1 Publication

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
7227.FBpp0292263

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q9Y118

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni64 – 80Necessary for function, possibly resulting from its inability to interact with Pol311 PublicationAdd BLAST17

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG410KB07 Eukaryota
ENOG4110RJT LUCA

KEGG Orthology (KO)

More...
KOi
K03504

Identification of Orthologs from Complete Genome Data

More...
OMAi
FVITQRK

Database of Orthologous Groups

More...
OrthoDBi
1384221at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q9Y118

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9Y118-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLKKALDDC MIDFDRCVLV TDLLEEYKLS YKEVNDVLEA YIKEQEPATK
60 70 80 90 100
FEKRFLVHGK RKTQGSDSGE DLYSVVLESR MQDWLAKVQD AESQLYSVKI
110 120 130 140 150
AGGTKAPAAI FKPMQHLEVK LAKVEQRPGA GKIVPSANGT TPHNGVKSEP
160 170 180 190 200
TKSEPSKSAV KLEPSKSSLK SEPAKSKAEK PVASKSSPED KKTSPKEQAS
210 220 230 240 250
KAKPAAAKKG SINSFFTAAA SKPKDVKATP SKSTSGTVDN FFKKQPAGAK
260 270 280 290 300
KSPPESEDKS KKDASNSNKK EASKKKSPSP TKKPTTANTS MQLFDEESAE
310 320 330 340 350
SSDEEEKLDM LRRKVIESDN DSDQEKASSS KRRRISDSED EEQPPKKSAD
360 370 380 390 400
EETIALDEKM DTEPANETYL DEDGFVITQR KPTKAQPANK KVSPKAAAPV
410 420 430
NKKKSPPSAA KAGKDAPKTK QAGIMNFFSK K
Length:431
Mass (Da):47,046
Last modified:November 1, 1999 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA8C8A0460E5CA7DC
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE014134 Genomic DNA Translation: AAF53445.1
AE014134 Genomic DNA Translation: ADV37066.1
FM245602 Genomic DNA Translation: CAR93528.1
FM245606 Genomic DNA Translation: CAR93532.1
FM245609 Genomic DNA Translation: CAR93535.1
AF145654 mRNA Translation: AAD38629.1

NCBI Reference Sequences

More...
RefSeqi
NP_001188816.1, NM_001201887.2
NP_609743.1, NM_135899.3

Genome annotation databases

Ensembl metazoan genome annotation project

More...
EnsemblMetazoai
FBtr0303164; FBpp0292263; FBgn0283467
FBtr0336864; FBpp0307818; FBgn0283467

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
34892

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
dme:Dmel_CG3975

UCSC genome browser

More...
UCSCi
CG3975-RA d. melanogaster

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA Translation: AAF53445.1
AE014134 Genomic DNA Translation: ADV37066.1
FM245602 Genomic DNA Translation: CAR93528.1
FM245606 Genomic DNA Translation: CAR93532.1
FM245609 Genomic DNA Translation: CAR93535.1
AF145654 mRNA Translation: AAD38629.1
RefSeqiNP_001188816.1, NM_001201887.2
NP_609743.1, NM_135899.3

3D structure databases

SMRiQ9Y118
ModBaseiSearch...

Protein-protein interaction databases

STRINGi7227.FBpp0292263

Proteomic databases

PaxDbiQ9Y118

Genome annotation databases

EnsemblMetazoaiFBtr0303164; FBpp0292263; FBgn0283467
FBtr0336864; FBpp0307818; FBgn0283467
GeneIDi34892
KEGGidme:Dmel_CG3975
UCSCiCG3975-RA d. melanogaster

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
34892
FlyBaseiFBgn0283467 Pol32

Phylogenomic databases

eggNOGiENOG410KB07 Eukaryota
ENOG4110RJT LUCA
KOiK03504
OMAiFVITQRK
OrthoDBi1384221at2759
PhylomeDBiQ9Y118

Miscellaneous databases

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
34892

Gene expression databases

BgeeiFBgn0027559 Expressed in 3 organ(s), highest expression level in larva

Family and domain databases

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDPOD3_DROME
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9Y118
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 11, 2019
Last sequence update: November 1, 1999
Last modified: December 11, 2019
This is version 155 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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