Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

V-type proton ATPase catalytic subunit A

Gene

vha-13

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (Probable). V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells (Probable). Required along with other vacuolar ATPase components for the removal of protein aggregates which form in immature oocytes in the distal gonad (PubMed:29168500). This removal occurs as the oocytes mature and move to the proximal gonad, is triggered by the introduction of sperm through mating and occurs before fertilization (PubMed:29168500). The introduction of sperm triggers V-ATPase accumulation in proximal oocytes and induces lysosomal acidification which leads to engulfing of protein aggregates by lysosomes and subsequent clearance of the aggregates (PubMed:29168500). Lysosomal acidification also leads to changes in mitochondrial morphology and function (PubMed:29168500). Mitochondria in distal immature oocytes are fragmented, produce high levels of reactive oxygen species (ROS) and have high membrane potential, indicative of metabolic inactivity (PubMed:29168500). In contrast, mitochondria in proximal mature oocytes are tubular with lower ROS levels and membrane potential, indicative of an active metabolic state required for aggregate mobilization before clearance (PubMed:29168500).Curated1 Publication

Catalytic activityi

ATP + H2O + 4 H+(Side 1) = ADP + phosphate + 4 H+(Side 2).Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi240 – 247ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • proton-transporting ATPase activity, rotational mechanism Source: GO_Central

GO - Biological processi

  • ATP hydrolysis coupled proton transport Source: InterPro
  • ATP metabolic process Source: InterPro
  • lysosomal lumen acidification Source: UniProtKB

Keywordsi

Molecular functionTranslocase
Biological processHydrogen ion transport, Ion transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-1222556 ROS, RNS production in phagocytes
R-CEL-77387 Insulin receptor recycling
R-CEL-917977 Transferrin endocytosis and recycling

Protein family/group databases

TCDBi3.A.2.2.7 the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase catalytic subunit A (EC:7.1.2.2)
Short name:
V-ATPase subunit A
Alternative name(s):
V-ATPase 69 kDa subunit
Vacuolar H ATPase protein 13
Vacuolar proton pump subunit alpha
Gene namesi
Name:vha-13Imported
ORF Names:Y49A3A.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiY49A3A.2 ; CE22210 ; WBGene00013025 ; vha-13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in increased protein aggregation in the oocytes of sperm-deficient young adult females which is not eliminated by mating.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002329032 – 606V-type proton ATPase catalytic subunit AAdd BLAST605

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9XW92
PaxDbiQ9XW92
PeptideAtlasiQ9XW92
PRIDEiQ9XW92

2D gel databases

World-2DPAGEi0020:Q9XW92

PTM databases

iPTMnetiQ9XW92

Expressioni

Tissue specificityi

Expressed in proximal but not distal germ cells.1 Publication

Gene expression databases

BgeeiWBGene00013025 Expressed in 5 organ(s), highest expression level in multi-cellular organism

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein).

Protein-protein interaction databases

STRINGi6239.Y49A3A.2.4

Structurei

3D structure databases

ProteinModelPortaliQ9XW92
SMRiQ9XW92
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Sequence analysis

Phylogenomic databases

eggNOGiKOG1352 Eukaryota
COG1155 LUCA
GeneTreeiENSGT00550000074787
HOGENOMiHOG000161057
InParanoidiQ9XW92
KOiK02145
OMAiQQLAKWS
OrthoDBiEOG091G03SR
PhylomeDBiQ9XW92

Family and domain databases

Gene3Di1.10.1140.10, 1 hit
2.40.30.20, 1 hit
HAMAPiMF_00309 ATP_synth_A_arch, 1 hit
InterProiView protein in InterPro
IPR031686 ATP-synth_a_Xtn
IPR023366 ATP_synth_asu-like_sf
IPR020003 ATPase_a/bsu_AS
IPR004100 ATPase_F1/V1/A1_a/bsu_N
IPR036121 ATPase_F1/V1/A1_a/bsu_N_sf
IPR000194 ATPase_F1/V1/A1_a/bsu_nucl-bd
IPR024034 ATPase_F1/V1_b/a_C
IPR005725 ATPase_V1-cplx_asu
IPR027417 P-loop_NTPase
IPR022878 V-ATPase_asu
PANTHERiPTHR43607 PTHR43607, 1 hit
PfamiView protein in Pfam
PF00006 ATP-synt_ab, 1 hit
PF02874 ATP-synt_ab_N, 1 hit
PF16886 ATP-synt_ab_Xtn, 1 hit
SUPFAMiSSF50615 SSF50615, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01042 V-ATPase_V1_A, 1 hit
PROSITEiView protein in PROSITE
PS00152 ATPASE_ALPHA_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XW92-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAESSYGFV YGVSGPVVTA EKMAGSAMYE LVRVGHQELV GEIIRLEGDY
60 70 80 90 100
ATIQVYEETS GVTIGDPVLR TGKPLSVELG PGIMGSIFDG IQRPLKDIAD
110 120 130 140 150
ITQSIYIPKG VSTNALSREA RWDFVVSKDL RVGGHVTGGD IIGTVDENLL
160 170 180 190 200
IKHKILLPPS ACGTITFVAP SGQYTVEDTL LELEFAGRKQ KFSMLQIWPV
210 220 230 240 250
RSPRPVTEKL AANNPLLCGQ RVLDALFPCV QGGTTAIPGA FGCGKTVISQ
260 270 280 290 300
SLSKYSNSDA IIYVGCGERG NEMSEVLRDF PELTMEVEGV TTSIMKRTAL
310 320 330 340 350
VANTSNMPVA AREASIYTGI TLAEYFRDMG LNVAMMADST SRWAEALREI
360 370 380 390 400
SGRLGEMPAD SGYPAYLAAR LASFYERAGR VKCLGSPERE GSVTIVGAVS
410 420 430 440 450
PPGGDFADPV TSATLGIVQV FWGLDKKLAQ RKHFPSINWL ISYSEYMRAL
460 470 480 490 500
EEFYEKNYPE FVSLRTKCKE ILQEEEDLSE IVQLVGKASL AESDKVTLEV
510 520 530 540 550
AKIIKDDFLQ QNGYTKYDRF CPFYKTVGML KNMIGFYDLA RHAVEATAQS
560 570 580 590 600
DNKITWNVIK DSMGDLIYQL SAMKFKDPVA DGEAKIRKDY EDLAEAMANA

FRNLED
Length:606
Mass (Da):66,460
Last modified:January 23, 2007 - v3
Checksum:iD1A73ECC57B85892
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL033512 Genomic DNA Translation: CAA22076.1
PIRiT27035
RefSeqiNP_506559.1, NM_074158.4
UniGeneiCel.38650

Genome annotation databases

EnsemblMetazoaiY49A3A.2.1; Y49A3A.2.1; WBGene00013025
Y49A3A.2.2; Y49A3A.2.2; WBGene00013025
Y49A3A.2.3; Y49A3A.2.3; WBGene00013025
Y49A3A.2.4; Y49A3A.2.4; WBGene00013025
Y49A3A.2.5; Y49A3A.2.5; WBGene00013025
GeneIDi3564970
KEGGicel:CELE_Y49A3A.2
UCSCiY49A3A.2.2 c. elegans

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL033512 Genomic DNA Translation: CAA22076.1
PIRiT27035
RefSeqiNP_506559.1, NM_074158.4
UniGeneiCel.38650

3D structure databases

ProteinModelPortaliQ9XW92
SMRiQ9XW92
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.Y49A3A.2.4

Protein family/group databases

TCDBi3.A.2.2.7 the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily

PTM databases

iPTMnetiQ9XW92

2D gel databases

World-2DPAGEi0020:Q9XW92

Proteomic databases

EPDiQ9XW92
PaxDbiQ9XW92
PeptideAtlasiQ9XW92
PRIDEiQ9XW92

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY49A3A.2.1; Y49A3A.2.1; WBGene00013025
Y49A3A.2.2; Y49A3A.2.2; WBGene00013025
Y49A3A.2.3; Y49A3A.2.3; WBGene00013025
Y49A3A.2.4; Y49A3A.2.4; WBGene00013025
Y49A3A.2.5; Y49A3A.2.5; WBGene00013025
GeneIDi3564970
KEGGicel:CELE_Y49A3A.2
UCSCiY49A3A.2.2 c. elegans

Organism-specific databases

CTDi3564970
WormBaseiY49A3A.2 ; CE22210 ; WBGene00013025 ; vha-13

Phylogenomic databases

eggNOGiKOG1352 Eukaryota
COG1155 LUCA
GeneTreeiENSGT00550000074787
HOGENOMiHOG000161057
InParanoidiQ9XW92
KOiK02145
OMAiQQLAKWS
OrthoDBiEOG091G03SR
PhylomeDBiQ9XW92

Enzyme and pathway databases

ReactomeiR-CEL-1222556 ROS, RNS production in phagocytes
R-CEL-77387 Insulin receptor recycling
R-CEL-917977 Transferrin endocytosis and recycling

Miscellaneous databases

PROiPR:Q9XW92

Gene expression databases

BgeeiWBGene00013025 Expressed in 5 organ(s), highest expression level in multi-cellular organism

Family and domain databases

Gene3Di1.10.1140.10, 1 hit
2.40.30.20, 1 hit
HAMAPiMF_00309 ATP_synth_A_arch, 1 hit
InterProiView protein in InterPro
IPR031686 ATP-synth_a_Xtn
IPR023366 ATP_synth_asu-like_sf
IPR020003 ATPase_a/bsu_AS
IPR004100 ATPase_F1/V1/A1_a/bsu_N
IPR036121 ATPase_F1/V1/A1_a/bsu_N_sf
IPR000194 ATPase_F1/V1/A1_a/bsu_nucl-bd
IPR024034 ATPase_F1/V1_b/a_C
IPR005725 ATPase_V1-cplx_asu
IPR027417 P-loop_NTPase
IPR022878 V-ATPase_asu
PANTHERiPTHR43607 PTHR43607, 1 hit
PfamiView protein in Pfam
PF00006 ATP-synt_ab, 1 hit
PF02874 ATP-synt_ab_N, 1 hit
PF16886 ATP-synt_ab_Xtn, 1 hit
SUPFAMiSSF50615 SSF50615, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01042 V-ATPase_V1_A, 1 hit
PROSITEiView protein in PROSITE
PS00152 ATPASE_ALPHA_BETA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVATA_CAEEL
AccessioniPrimary (citable) accession number: Q9XW92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 130 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again