UniProtKB - Q9XUB3 (REC8_CAEEL)
Meiotic recombination protein rec-8
rec-8
Functioni
Plays a role in meiotic chromosome cohesion and segregation (PubMed:11390355, PubMed:25171895).
Necessary for sister chromatid co-orientation and sister chromatid cohension until meiosis II (PubMed:19574299).
May also be required for repair of programmed meiotic double stranded breaks (PubMed:11390355).
During spermatogenesis, likely involved in spermatocyte centriole cohesion, thus preventing their separation during the second meiotic division (PubMed:23401519).
4 PublicationsGO - Molecular functioni
- chromatin binding Source: GO_Central
- damaged DNA binding Source: WormBase
GO - Biological processi
- double-strand break repair Source: WormBase
- meiotic cell cycle Source: WormBase
- meiotic chromosome movement towards spindle pole Source: WormBase
- meiotic sister chromatid cohesion Source: WormBase
- mitotic sister chromatid cohesion Source: GO_Central
- reciprocal meiotic recombination Source: WormBase
- regulation of centriole-centriole cohesion Source: UniProtKB
- replication-born double-strand break repair via sister chromatid exchange Source: GO_Central
- synaptonemal complex assembly Source: WormBase
Keywordsi
Biological process | DNA damage, DNA repair, Meiosis |
Names & Taxonomyi
Protein namesi | Recommended name: Meiotic recombination protein rec-8By similarityAlternative name(s): Cohesin rec-8By similarity |
Gene namesi | Name:rec-8Imported ORF Names:W02A2.6Imported |
Organismi | Caenorhabditis elegansImported |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | W02A2.6a ; CE21236 ; WBGene00004333 ; rec-8 W02A2.6b ; CE45697 ; WBGene00004333 ; rec-8 W02A2.6c ; CE45566 ; WBGene00004333 ; rec-8 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Cytoskeleton
- spindle pole 1 Publication
Other locations
- Chromosome 7 Publications
Note: Colocalizes with smc-1 along synapsed chromosomes during meiotic pachytene and diakinesis (PubMed:12827206). Loaded onto chromosomes upon entry into meiosis (PubMed:21856158). Localizes to chromosomes until meiotic anaphase II and to microtubules throughout meiotic divisions (PubMed:23401519).3 Publications
Cytoskeleton
- meiotic spindle pole Source: UniProtKB
- spindle microtubule Source: UniProtKB
Nucleus
- condensed nuclear chromosome Source: WormBase
- nuclear meiotic cohesin complex Source: GO_Central
- nuclear mitotic cohesin complex Source: GO_Central
- nucleoplasm Source: WormBase
- nucleus Source: WormBase
- synaptonemal complex Source: WormBase
Other locations
- chromatin Source: WormBase
- chromosome Source: UniProtKB
- cytoplasm Source: UniProtKB-KW
Keywords - Cellular componenti
Chromosome, Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 625 | T → A: Reduced overall phosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000432844 | 1 – 781 | Meiotic recombination protein rec-8CuratedAdd BLAST | 781 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 625 | Phosphothreonine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9XUB3 |
PaxDbi | Q9XUB3 |
PeptideAtlasi | Q9XUB3 |
PTM databases
iPTMneti | Q9XUB3 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | WBGene00004333, Expressed in adult organism and 5 other tissues |
Interactioni
Protein-protein interaction databases
DIPi | DIP-25674N |
IntActi | Q9XUB3, 1 interactor |
STRINGi | 6239.W02A2.6a |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 374 – 418 | DisorderedSequence analysisAdd BLAST | 45 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 374 – 395 | Basic and acidic residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502TG65, Eukaryota |
HOGENOMi | CLU_017209_0_0_1 |
InParanoidi | Q9XUB3 |
OMAi | ANFHENI |
OrthoDBi | 1463478at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR039781, Rad21/Rec8-like IPR006910, Rad21_Rec8_N |
PANTHERi | PTHR12585, PTHR12585, 1 hit |
Pfami | View protein in Pfam PF04825, Rad21_Rec8_N, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVVSAEVIRK DAVFHVAWIL GTGDSKKLSR REILDQNLPE LCHSIIEMVP
60 70 80 90 100
ERHRGSATKT GLYLLSLLTY GTVLIHQVQV DFLKRDVEKL KELMKKKSFI
110 120 130 140 150
LLMAERFDRN QELQRKEDKF ARLRSKPIMC VEELDRVDLA HLQAIGDELG
160 170 180 190 200
INGNPGDFIM MDALPNMNQW IDNNSELNAI YGCVEPYLRE KEITMHSTFV
210 220 230 240 250
EGNGSNEHNK ERRNDAVIAD FSQLLFPEIP EITLGEKFPI DVDSRKRSAI
260 270 280 290 300
LQEEQEEALQ LPKEASEIVQ EEPTKFVSIA LLPSETVEQP APQEPIQEPI
310 320 330 340 350
QPIIEEPAPQ LELPQPELPP QLDAIDLVTI PASQQDMVVE YLQLINDLPD
360 370 380 390 400
DENSRLPPLP KDLELFEDVI LPPPAKKSKV EEEEDALERA RRRPSSRPVT
410 420 430 440 450
PINQTDLTDL HSTVRPEDPS FAIDSQIHDV LPQRKKSKRN LPIIHSDDLE
460 470 480 490 500
IDEAVQKVLQ ADYSSLVRKK EDVIAKIPPK TDAVAVLMNL PEPVFSIGYR
510 520 530 540 550
LPPEVRDMFK ACYNQAVGSP VSDDEEDEDE EEEEEYKYAK VCLLSPNRIV
560 570 580 590 600
EDTLLLEEQP RQPEEFPSTD NINPPRQLQE NPVFENLEYE APPHPIRTAR
610 620 630 640 650
TPTPIKDLKY SVISLFPTPE KRRETSIIAE LNLDPIPVEE IDPLLTMRTE
660 670 680 690 700
EELENVRRRQ KSSLGVQFMR TDDLEEDTRR NRLFEDEERT RDAREDELFF
710 720 730 740 750
YSSGSLLPNN RLNIHKELLN EAEARYPEWV NFNEFTADHD RKKAATAFEG
760 770 780
LLLSLKNMKV EAKQEDPYFP ILVRHISHEE M
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057600 | 1 – 757 | Missing in isoform c. CuratedAdd BLAST | 757 | |
Alternative sequenceiVSP_057601 | 1 – 194 | Missing in isoform b. CuratedAdd BLAST | 194 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z82286 Genomic DNA Translation: CAB05309.1 Z82286 Genomic DNA Translation: CBY25206.1 Z82286 Genomic DNA Translation: CBY25207.1 |
PIRi | T26080 |
RefSeqi | NP_001255727.1, NM_001268798.1 [Q9XUB3-1] NP_001255728.1, NM_001268799.1 [Q9XUB3-2] NP_001255729.1, NM_001268800.1 [Q9XUB3-3] |
Genome annotation databases
EnsemblMetazoai | W02A2.6a.1; W02A2.6a.1; WBGene00004333 [Q9XUB3-1] W02A2.6b.1; W02A2.6b.1; WBGene00004333 [Q9XUB3-2] W02A2.6c.1; W02A2.6c.1; WBGene00004333 [Q9XUB3-3] |
GeneIDi | 178295 |
KEGGi | cel:CELE_W02A2.6 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z82286 Genomic DNA Translation: CAB05309.1 Z82286 Genomic DNA Translation: CBY25206.1 Z82286 Genomic DNA Translation: CBY25207.1 |
PIRi | T26080 |
RefSeqi | NP_001255727.1, NM_001268798.1 [Q9XUB3-1] NP_001255728.1, NM_001268799.1 [Q9XUB3-2] NP_001255729.1, NM_001268800.1 [Q9XUB3-3] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
DIPi | DIP-25674N |
IntActi | Q9XUB3, 1 interactor |
STRINGi | 6239.W02A2.6a |
PTM databases
iPTMneti | Q9XUB3 |
Proteomic databases
EPDi | Q9XUB3 |
PaxDbi | Q9XUB3 |
PeptideAtlasi | Q9XUB3 |
Genome annotation databases
EnsemblMetazoai | W02A2.6a.1; W02A2.6a.1; WBGene00004333 [Q9XUB3-1] W02A2.6b.1; W02A2.6b.1; WBGene00004333 [Q9XUB3-2] W02A2.6c.1; W02A2.6c.1; WBGene00004333 [Q9XUB3-3] |
GeneIDi | 178295 |
KEGGi | cel:CELE_W02A2.6 |
Organism-specific databases
CTDi | 178295 |
WormBasei | W02A2.6a ; CE21236 ; WBGene00004333 ; rec-8 W02A2.6b ; CE45697 ; WBGene00004333 ; rec-8 W02A2.6c ; CE45566 ; WBGene00004333 ; rec-8 |
Phylogenomic databases
eggNOGi | ENOG502TG65, Eukaryota |
HOGENOMi | CLU_017209_0_0_1 |
InParanoidi | Q9XUB3 |
OMAi | ANFHENI |
OrthoDBi | 1463478at2759 |
Miscellaneous databases
PROi | PR:Q9XUB3 |
Gene expression databases
Bgeei | WBGene00004333, Expressed in adult organism and 5 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR039781, Rad21/Rec8-like IPR006910, Rad21_Rec8_N |
PANTHERi | PTHR12585, PTHR12585, 1 hit |
Pfami | View protein in Pfam PF04825, Rad21_Rec8_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | REC8_CAEEL | |
Accessioni | Q9XUB3Primary (citable) accession number: Q9XUB3 Secondary accession number(s): E5QCH7, E5QCH8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2015 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families