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Protein

Zinc finger protein mex-5

Gene

mex-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions with mex-6 to affect embryonic viability, establish soma germline asymmetry in embryos and establish plk-1, pie-1, mex-1, and pos-1 asymmetry in embryos (PubMed:10882103, PubMed:18199581, PubMed:18842813). Also affects formation of intestinal cells (PubMed:10882103). Binds to mRNA in vitro, and inhibits pgl-3-mediated P-granule formation, probably by competing with pgl-3 for binding to mRNA (PubMed:27594427).4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri270 – 299C3H1-type 1PROSITE-ProRule annotationAdd BLAST30
Zinc fingeri314 – 344C3H1-type 2PROSITE-ProRule annotationAdd BLAST31

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • mRNA 3'-UTR binding Source: WormBase
  • poly-pyrimidine tract binding Source: WormBase
  • protein domain specific binding Source: WormBase
  • protein kinase binding Source: WormBase

GO - Biological processi

  • 3'-UTR-mediated mRNA destabilization Source: GO_Central
  • multicellular organism development Source: UniProtKB-KW
  • regulation of protein localization Source: WormBase

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, RNA-binding
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein mex-5
Gene namesi
Name:mex-5Imported
ORF Names:W02A2.7Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiW02A2.7 ; CE21237 ; WBGene00003230 ; mex-5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown in mex-6 pk440 mutant background causes a loss in plk-1 asymmetric distribution during the first embryonic cell divisions.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi186T → A: Abolishes phosphorylation. Reduced embryo viability and, pie-1 and mex-5 degradation in embryonic somatic cells. Severe reduction in binding to plk-1 and plk-2. Prevent subsequent phosphorylation by plk-1. 1 Publication1
Mutagenesisi186T → D or E: Phosphomimetic mutant which severely abolishes interaction with plk-1 and plk-2. 1 Publication1
Mutagenesisi458S → A: Loss of phosphorylation and zygotic asymmetry. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1293319

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000891831 – 468Zinc finger protein mex-5Add BLAST468

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186Phosphothreonine; by mbk-21 Publication1
Modified residuei458Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation on Ser-458 by par-1 promotes localization of the protein to the anterior cytoplasm of the zygote (PubMed:18842813). Phosphorylation by mbk-1 appears to be required for subsequent phosphorylation by plk-1 (PubMed:18199581).2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9XUB2
PaxDbiQ9XUB2
PeptideAtlasiQ9XUB2
PRIDEiQ9XUB2

PTM databases

iPTMnetiQ9XUB2

Expressioni

Tissue specificityi

Asymmetrically localized to the anterior of the zygote before mitotic division, then differentially distributed to the somatic blastomere precursor cells.2 Publications

Gene expression databases

BgeeiWBGene00003230

Interactioni

Subunit structurei

Interacts (when phosphorylated on Thr-186) with plk-1 (via POLO box domain) and plk-2 (via POLO box domain).1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: WormBase
  • protein kinase binding Source: WormBase

Protein-protein interaction databases

BioGridi43384, 8 interactors
IntActiQ9XUB2, 3 interactors
STRINGi6239.W02A2.7.2

Chemistry databases

BindingDBiQ9XUB2

Structurei

3D structure databases

ProteinModelPortaliQ9XUB2
SMRiQ9XUB2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri270 – 299C3H1-type 1PROSITE-ProRule annotationAdd BLAST30
Zinc fingeri314 – 344C3H1-type 2PROSITE-ProRule annotationAdd BLAST31

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1677 Eukaryota
COG5063 LUCA
GeneTreeiENSGT00840000131885
HOGENOMiHOG000113570
InParanoidiQ9XUB2
OMAiYHRVMEH
OrthoDBiEOG091G0D7J

Family and domain databases

InterProiView protein in InterPro
IPR000571 Znf_CCCH
IPR036855 Znf_CCCH_sf
PfamiView protein in Pfam
PF00642 zf-CCCH, 2 hits
SMARTiView protein in SMART
SM00356 ZnF_C3H1, 2 hits
SUPFAMiSSF90229 SSF90229, 2 hits
PROSITEiView protein in PROSITE
PS50103 ZF_C3H1, 2 hits

Sequencei

Sequence statusi: Complete.

Q9XUB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAASNSVSS AGGSVSPTTT QPPLPPGQSS HPQIYDQQMQ YYFAAAMPNQ
60 70 80 90 100
PMATYAAQNG SSQQYAPAAP YYQDANGQYV QVPANGSMAP QQHMMVSGQP
110 120 130 140 150
YLYMAQPQQG AQQVMQSGQP QLIYYQQSMA PQAAPMYFHP MQAAPMLPEQ
160 170 180 190 200
MGVMPHTQPA IPPQQQPRQV GVEISSTRTA PLTSSTPLPT SLEYETVQRD
210 220 230 240 250
NRNRNIQFRY HRVMEHDELP IDEISKITLD NHNDDTMSAE KENHFHEHRG
260 270 280 290 300
EKFGRRGFPI PETDSQQPPN YKTRLCMMHA SGIKPCDMGA RCKFAHGLKE
310 320 330 340 350
LRATDAPARY PNNKYKTKLC KNFARGGTGF CPYGLRCEFV HPTDKEFQNI
360 370 380 390 400
PPYQRMSHDD QDYDQDVIPE DYVVARHQPR FMRTGGRATT PTKVMLKHRN
410 420 430 440 450
VAGSMMCLSN AGRDLQAGGD YNQPESNEDD LPPHLRRNRR ENPPMNKRRT
460
SLSTKWTSEE NLGLRGHY
Length:468
Mass (Da):52,820
Last modified:November 1, 1999 - v1
Checksum:i6B0E6FC4971CF5CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z82286 Genomic DNA Translation: CAB05310.1
PIRiT26081
RefSeqiNP_502566.1, NM_070165.4
UniGeneiCel.23008

Genome annotation databases

EnsemblMetazoaiW02A2.7; W02A2.7; WBGene00003230
GeneIDi178296
KEGGicel:CELE_W02A2.7
UCSCiW02A2.7 c. elegans

Similar proteinsi

Entry informationi

Entry nameiMEX5_CAEEL
AccessioniPrimary (citable) accession number: Q9XUB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1999
Last modified: March 28, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase

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