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Protein

Serine/threonine-protein kinase akt-2

Gene

akt-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts downstream of PI3 kinase age-1 and kinase pdk-1 in the daf-2/insulin receptor-like transduction pathway (PubMed:9716402, PubMed:10364160, PubMed:11747825, PubMed:11381260, PubMed:18782349, PubMed:15068796). Essential role in regulating developmental arrest at the dauer stage (PubMed:10364160). Phosphorylates Forkhead-related daf-16 and the longevity-promoting skn-1 transcription factors, which inhibits their entry into the nucleus and antagonizes their functions (PubMed:18358814, PubMed:11747825, PubMed:11381260, PubMed:15068796). Role in immune function and pathogen resistance (PubMed:18782349). Downstream of age-1 and together with akt-2 and sgk-1, promotes cell survival during embryonic development (PubMed:25383666).8 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Phosphorylated and activated by pdk-1.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei209ATPPROSITE-ProRule annotation1
Active sitei303Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi186 – 194ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: WormBase
  • protein kinase activity Source: WormBase
  • protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  • determination of adult lifespan Source: WormBase
  • innate immune response Source: UniProtKB-KW
  • insulin receptor signaling pathway Source: WormBase
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: WormBase
  • peptidyl-threonine phosphorylation Source: WormBase
  • protein phosphorylation Source: WormBase
  • regulation of synaptic growth at neuromuscular junction Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processImmunity, Innate immunity
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-1257604 PIP3 activates AKT signaling
R-CEL-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
R-CEL-165158 Activation of AKT2
R-CEL-165181 Inhibition of TSC complex formation by PKB
R-CEL-198323 AKT phosphorylates targets in the cytosol
R-CEL-198693 AKT phosphorylates targets in the nucleus
R-CEL-199418 Negative regulation of the PI3K/AKT network
R-CEL-203615 eNOS activation
R-CEL-211163 AKT-mediated inactivation of FOXO1A
R-CEL-389357 CD28 dependent PI3K/Akt signaling
R-CEL-389513 CTLA4 inhibitory signaling
R-CEL-392451 G beta:gamma signalling through PI3Kgamma
R-CEL-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA
R-CEL-450604 KSRP (KHSRP) binds and destabilizes mRNA
R-CEL-5218920 VEGFR2 mediated vascular permeability
R-CEL-5628897 TP53 Regulates Metabolic Genes
R-CEL-6804758 Regulation of TP53 Activity through Acetylation
R-CEL-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-CEL-8849469 PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1
R-CEL-8876198 RAB GEFs exchange GTP for GDP on RABs
R-CEL-8948751 Regulation of PTEN stability and activity
SignaLinkiQ9XTG7

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase akt-2 (EC:2.7.11.1)
Alternative name(s):
Protein kinase B akt-2
Short name:
PKB akt-2
Gene namesi
Name:akt-2
ORF Names:F28H6.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF28H6.1a ; CE18646 ; WBGene00000103 ; akt-2
F28H6.1b ; CE29298 ; WBGene00000103 ; akt-2

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Defective egg-laying and increased resistance to pathogens. Simultaneous knockdown of akt-1 and akt-2 result in dauer formation and a weak extension to life span.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi209K → M: Probable loss of kinase activity. Increased apoptosis during embryonic development in an akt-1 tm399 mutant background. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856161 – 528Serine/threonine-protein kinase akt-2Add BLAST528

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9XTG7
PeptideAtlasiQ9XTG7
PRIDEiQ9XTG7

PTM databases

iPTMnetiQ9XTG7

Expressioni

Tissue specificityi

Expressed in neurons, muscle cells of the pharynx, rectal gland cells, and spermatheca.1 Publication

Developmental stagei

Expressed in late stage embryos and throughout life.1 Publication

Gene expression databases

BgeeiWBGene00000103

Interactioni

Subunit structurei

Interacts with pdk-1, sgk-1, akt-1 and daf-16. Part of a complex containing sgk-1, akt-1 and akt-2.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi46423, 6 interactors
ComplexPortaliCPX-1129 Atk-1/Akt-2/Sgk-1 protein kinase complex
DIPiDIP-26368N
IntActiQ9XTG7, 12 interactors
STRINGi6239.F28H6.1a

Structurei

3D structure databases

ProteinModelPortaliQ9XTG7
SMRiQ9XTG7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 115PHPROSITE-ProRule annotationAdd BLAST104
Domaini180 – 437Protein kinasePROSITE-ProRule annotationAdd BLAST258
Domaini438 – 515AGC-kinase C-terminalAdd BLAST78

Domaini

The PH domain binds to phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) resulting in its targeting to the plasma membrane.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0598 Eukaryota
ENOG410XNPH LUCA
GeneTreeiENSGT00920000148938
HOGENOMiHOG000233033
InParanoidiQ9XTG7
KOiK04456
OMAiGTYYAMK
OrthoDBiEOG091G06FF
PhylomeDBiQ9XTG7

Family and domain databases

CDDicd01241 PH_PKB, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR011009 Kinase-like_dom_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR039026 PH_PKB
IPR017892 Pkinase_C
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR039029 RAC_gamma-like
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR24356:SF190 PTHR24356:SF190, 1 hit
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q9XTG7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTENAHLQK EDIVIESWLH KKGEHIRNWR PRYFILFRDG TLLGFRSKPK
60 70 80 90 100
EDQPLPEPLN NFMIRDAATV CLDKPRPNMF IVRCLQWTTV IERTFYADSA
110 120 130 140 150
DFRQMWIEAI QAVSSHNRLK ENAGNTSMQE EDTNGNPSGE SDVNMDATST
160 170 180 190 200
RSDNDFESTV MNIDEPEEVP RKNTVTMDDF DFLKVLGQGT FGKVILCREK
210 220 230 240 250
SSDKLYAIKI IRKEMVVDRS EVAHTLTENR VLYACVHPFL TLLKYSFQAQ
260 270 280 290 300
YHICFVMEFA NGGELFTHLQ RCKTFSEART RFYGSEIILA LGYLHHRNIV
310 320 330 340 350
YRDMKLENLL LDRDGHIKIT DFGLCKEEIK YGDKTSTFCG TPEYLAPEVI
360 370 380 390 400
EDIDYDRSVD WWGVGVVMYE MMCGRLPFSA KENGKLFELI TTCDLKFPNR
410 420 430 440 450
LSPEAVTLLS GLLERVPAKR LGAGPDDARE VSRAEFFKDV DWEATLRKEV
460 470 480 490 500
EPPFKPNVMS ETDTSFFDRE FTSMPVQLTP PRRGEELPTV DEEEELQANF
510 520
IQFASYYVSG SLERSYDTNR SADKYEIR
Note: No experimental confirmation available.
Length:528
Mass (Da):61,058
Last modified:November 1, 1999 - v1
Checksum:i9750E8F0AE4F6AE7
GO
Isoform b (identifier: Q9XTG7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     470-483: EFTSMPVQLTPPRR → VRYVSILLKVSEAI
     484-528: Missing.

Show »
Length:483
Mass (Da):55,757
Checksum:i7977EE98BF40CD83
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017047470 – 483EFTSM…TPPRR → VRYVSILLKVSEAI in isoform b. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_017048484 – 528Missing in isoform b. 1 PublicationAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072381 mRNA Translation: AAC62468.1
AL031621, Z92837 Genomic DNA Translation: CAA20936.1
AL031621, Z92837 Genomic DNA Translation: CAC70087.1
PIRiT21523
T43234
RefSeqiNP_001024612.1, NM_001029441.3 [Q9XTG7-2]
NP_510357.3, NM_077956.5 [Q9XTG7-1]
UniGeneiCel.18147

Genome annotation databases

EnsemblMetazoaiF28H6.1a; F28H6.1a; WBGene00000103 [Q9XTG7-1]
GeneIDi181524
KEGGicel:CELE_F28H6.1
UCSCiF28H6.1b c. elegans [Q9XTG7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAKT2_CAEEL
AccessioniPrimary (citable) accession number: Q9XTG7
Secondary accession number(s): O77145
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: November 1, 1999
Last modified: July 18, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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