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Protein

Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2

Gene

mbk-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Required for oocyte-to-zygote transition in which it phosphorylates oocyte proteins, including mei-1, oma-1, oma-2, mex-5, and mex-6, modifying their activity and/or stability following meiosis (PubMed:16289132, PubMed:16338136, PubMed:17869113, PubMed:18854162, PubMed:18199581, PubMed:19879842). Functions in both spindle positioning and in the posterior localization of cytoplasmic determinants, including pie-1, pos-1, and pgl-1, in early embryos (PubMed:14697358). Involved in the asymmetric distribution of plk-1 at the 2-cell embryonic stage (PubMed:18199581).7 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+3 Publications

<p>This subsection of the ‘Function’ section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activated during oocyte maturation by phosphorylation on Ser-362 by cdk-1. The pseudotyrosine phosphatases egg-4 and egg-5 sequester activated mbk-2 until the meiotic divisions and inhibit mbk-2 kinase activity directly, using a mixed-inhibition mechanism that does not involve tyrosine dephosphorylation.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

The presence of egg-4 in the assay increases the KM of mbk-2 for mei-1.1 Publication
  1. KM=0.3 µM for mei-1 (Isoform a)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Function’ section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei490ATPPROSITE-ProRule annotation3 Publications1
    <p>This subsection of the ‘Function’ section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei587Proton acceptorPROSITE-ProRule annotationBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Function’ section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi467 – 475ATPPROSITE-ProRule annotationBy similarity9

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    • ATP binding Source: WormBase
    • protein kinase activity Source: WormBase
    • protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
    • protein serine/threonine kinase activity Source: WormBase
    • protein tyrosine kinase activity Source: UniProtKB

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
    LigandATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    Reactome - a knowledgebase of biological pathways and processes

    More...
    Reactomei
    R-CEL-6804756 Regulation of TP53 Activity through Phosphorylation

    SignaLink: a signaling pathway resource with multi-layered regulatory networks

    More...
    SignaLinki
    Q9XTF3

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2By similarity (EC:2.7.12.13 Publications)
    Alternative name(s):
    Dual specificity Yak1-related kinase mbk-2By similarity
    Minibrain Kinase 21 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:mbk-2
    ORF Names:F49E11.1
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiCaenorhabditis elegans
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri6239 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001940 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome IV

    Organism-specific databases

    WormBase

    More...
    WormBasei
    F49E11.1a ; CE05897 ; WBGene00003150 ; mbk-2
    F49E11.1b ; CE19878 ; WBGene00003150 ; mbk-2
    F49E11.1c ; CE23751 ; WBGene00003150 ; mbk-2
    F49E11.1d ; CE39735 ; WBGene00003150 ; mbk-2
    F49E11.1e ; CE39938 ; WBGene00003150 ; mbk-2
    F49E11.1f ; CE47098 ; WBGene00003150 ; mbk-2
    F49E11.1g ; CE47427 ; WBGene00003150 ; mbk-2
    F49E11.1h ; CE47790 ; WBGene00003150 ; mbk-2

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Maternal-effect embryonic lethality due to defects in spindle positioning and cytokinesis in the early embryo (PubMed:12618396, PubMed:14697358). Microtubules are fragmented and disordered (PubMed:14697358). Abolishes phosphorylation of RNA-binding protein oma-1 in embryos (PubMed:16289132). RNAi-mediated knockdown causes an increase in taf-4 nuclear localization in the one-cell embryo (PubMed:18854162). RNAi-mediated knockdown causes a loss in plk-1 asymmetric localization in 2-cell stage embryo without affecting mex-5 polarization (PubMed:18199581).5 Publications

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi362S → A: Loss of phosphorylation by cdk-1. Loss of kinase activity. 1 Publication1
    Mutagenesisi362S → E: Constitutively active. 1 Publication1
    Mutagenesisi490K → R: Loss of autophosphorylation activity. Slight loss of binding to egg-4 and egg-5. 3 Publications1
    Mutagenesisi619Y → F: Reduced binding to egg-4 and egg-5 and translocation to the cytoplasm; when associated with F-621. 1 Publication1
    Mutagenesisi621Y → F: Loss of tyrosine phosphorylation. Reduced binding to egg-4 and egg-5 and translocation to the cytoplasm; when associated with F-619. 1 Publication1
    Mutagenesisi764T → A: No loss of phosphorylation by cdk-1. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003907171 – 817Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2Add BLAST817

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei362Phosphoserine; by cdk-11 Publication1
    Modified residuei621Phosphotyrosine; by autocatalysis1 Publication1

    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

    Autophosphorylated.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    Encyclopedia of Proteome Dynamics

    More...
    EPDi
    Q9XTF3

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    Q9XTF3

    PeptideAtlas

    More...
    PeptideAtlasi
    Q9XTF3

    PRoteomics IDEntifications database

    More...
    PRIDEi
    Q9XTF3

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...
    iPTMneti
    Q9XTF3

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

    In L1 larvae, expressed widely in the nervous system, including head neurons and the ventral nerve cord. In adult animals, continues to be expressed in the nervous system and is also expressed in body wall muscle.1 Publication

    <p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

    Expressed both maternally and zygotically.1 Publication

    Gene expression databases

    Bgee dataBase for Gene Expression Evolution

    More...
    Bgeei
    WBGene00003150 Expressed in 5 organ(s), highest expression level in multi-cellular organism

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Part of a complex, consisting of pseudophosphatases egg-3, egg-4, egg-5 and kinase mbk-2; this complex is required for the oocyte-to-zygote transition (PubMed:19879842). Interacts (via Tyr-619 and Tyr-621) with egg-4 (via tyrosine-protein phosphatase domain) and egg-5 (via tyrosine-protein phosphatase domain); mbk-2 tyrosine phosphorylation enhances the interaction (PubMed:19879842). The interaction inhibits mbk-2 kinase activity and is required for mbk-2 oocyte cortex localization. Interacts (via N-terminus) with egg-3 (via tyrosine-protein phosphatase domain); the interaction does not affect mbk-2 kinase activity, is enhanced by mbk-2 tyrosine phosphorylation status and requires prior binding of mbk-2 to egg-4 and egg-5 (PubMed:17869113, PubMed:19879842).2 Publications

    <p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGrid)

    More...
    BioGridi
    43342, 9 interactors

    ComplexPortal: manually curated resource of macromolecular complexes

    More...
    ComplexPortali
    CPX-3381 Egg-3/4/5 MBK-2 complex

    Protein interaction database and analysis system

    More...
    IntActi
    Q9XTF3, 11 interactors

    STRING: functional protein association networks

    More...
    STRINGi
    6239.F49E11.1b

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

    More...
    ProteinModelPortali
    Q9XTF3

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q9XTF3

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini461 – 774Protein kinasePROSITE-ProRule annotationAdd BLAST314

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi194 – 258Gln-richSequence analysisAdd BLAST65
    Compositional biasi307 – 391Ser-richSequence analysisAdd BLAST85

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG0667 Eukaryota
    ENOG410XPET LUCA

    Ensembl GeneTree

    More...
    GeneTreei
    ENSGT00940000166363

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    Q9XTF3

    KEGG Orthology (KO)

    More...
    KOi
    K18669

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    NTASTHD

    Database of Orthologous Groups

    More...
    OrthoDBi
    EOG091G0Q46

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    Q9XTF3

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR011009 Kinase-like_dom_sf
    IPR000719 Prot_kinase_dom
    IPR017441 Protein_kinase_ATP_BS
    IPR008271 Ser/Thr_kinase_AS

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00069 Pkinase, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00220 S_TKc, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF56112 SSF56112, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00107 PROTEIN_KINASE_ATP, 1 hit
    PS50011 PROTEIN_KINASE_DOM, 1 hit
    PS00108 PROTEIN_KINASE_ST, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (8+)i

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    This entry describes 8 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 8 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

    Isoform b1 Publication (identifier: Q9XTF3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MAALASFTRN SRSYGQQPID VTQQGQRDRS VMSLDAQGRS VSHECPTSTT
    60 70 80 90 100
    LVRQLYLPQI PQSASFAAAP TSFSGASSSS SNHHHPVYHS QNSLPPNLLG
    110 120 130 140 150
    SSQNSASSNS LVQGHRNPAL GSGNTLTRSY HQPSSTNSST NNLYGPLGTI
    160 170 180 190 200
    SRDLKQSIRD ISPPVINSSA NPHLVNYVQT SSFDNGSYEF PSGQAQQQRR
    210 220 230 240 250
    LGGSQQHLAP LQQTASSLYS NPQSSSSQLL GQQQAVRPNY AYQQSLPRQQ
    260 270 280 290 300
    HINSHQTQAF FGTVRGPTNS TNIVTPLRAS KTMIDVLAPV RDTVAAQATG
    310 320 330 340 350
    LPNVGTSSSN GSSNSSSGVG SGGSGSLMTQ SIGGPNKHLS ASHSTLNTAS
    360 370 380 390 400
    THDMMHSKIP KSPSNESLSR SHTSSSGGSQ GGHNSNSGSN SGFRPEDAVQ
    410 420 430 440 450
    TFGAKLVPFE KNEIYNYTRV FFVGSHAKKQ AGVIGGANNG GYDDENGSYQ
    460 470 480 490 500
    LVVHDHIAYR YEVLKVIGKG SFGQVIKAFD HKYQQYVALK LVRNEKRFHR
    510 520 530 540 550
    QADEEIRILD HLRRQDSDGT HNIIHMLDYF NFRNHKCITF ELLSINLYEL
    560 570 580 590 600
    IKRNKFQGFS LMLVRKFAYS MLLCLDLLQK NRLIHCDLKP ENVLLKQQGR
    610 620 630 640 650
    SGIKVIDFGS SCFDDQRIYT YIQSRFYRAP EVILGTKYGM PIDMWSLGCI
    660 670 680 690 700
    LAELLTGYPL LPGEDENDQL ALIIELLGMP PPKSLETAKR ARTFITSKGY
    710 720 730 740 750
    PRYCTATSMP DGSVVLAGAR SKRGKMRGPP ASRSWSTALK NMGDELFVDF
    760 770 780 790 800
    LKRCLDWDPE TRMTPAQALK HKWLRRRLPN PPRDGLESMG GLADHEKKTE
    810
    TLPNIDSNAN ILMRKKF
    Note: No experimental confirmation available.Curated
    Length:817
    Mass (Da):89,885
    Last modified:November 1, 1999 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i74903C85CB68C428
    GO
    Isoform a1 Publication (identifier: Q9XTF3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-294: Missing.
         295-310: AAQATGLPNVGTSSSN → MTLFEPSTSGNRMGYR
         797-817: KKTETLPNIDSNANILMRKKF → VCFIIF

    Note: No experimental confirmation available.
    Show »
    Length:508
    Mass (Da):56,841
    Checksum:i3EC0F264B291AC2C
    GO
    Isoform c2 Publications (identifier: Q9XTF3-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         797-817: Missing.

    Show »
    Length:796
    Mass (Da):87,441
    Checksum:iFE8365EC2FDE8D5A
    GO
    Isoform d1 Publication (identifier: Q9XTF3-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-327: Missing.
         797-817: KKTETLPNIDSNANILMRKKF → VCFIIF

    Note: No experimental confirmation available.Curated
    Show »
    Length:475
    Mass (Da):53,646
    Checksum:iFBDA51EA9638F7F0
    GO
    Isoform e1 Publication (identifier: Q9XTF3-5) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-301: Missing.
         302-310: PNVGTSSSN → MYSSLFELR
         797-817: KKTETLPNIDSNANILMRKKF → VCFIIF

    Note: No experimental confirmation available.Curated
    Show »
    Length:501
    Mass (Da):56,139
    Checksum:i3BA892EA8A8B8777
    GO
    Isoform f (identifier: Q9XTF3-6) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-297: Missing.
         298-310: ATGLPNVGTSSSN → MFAIPFHRFYSDE
         797-802: KKTETL → VCFIIF
         803-817: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:505
    Mass (Da):56,654
    Checksum:iC07154636CC01114
    GO
    Isoform g (identifier: Q9XTF3-7) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         148-164: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:800
    Mass (Da):88,020
    Checksum:i111F8423FBE140FC
    GO
    Isoform h (identifier: Q9XTF3-8) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-282: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:535
    Mass (Da):59,500
    Checksum:iF73E4415D844DE58
    GO

    <p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

    There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    A0A061AJ20A0A061AJ20_CAEEL
    Dual specificity tyrosine-phosphory...
    mbk-2 CELE_F49E11.1, F49E11.1
    469Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    A0A061ADU8A0A061ADU8_CAEEL
    Dual specificity tyrosine-phosphory...
    mbk-2 CELE_F49E11.1, F49E11.1
    490Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    A0A061ACK4A0A061ACK4_CAEEL
    Dual specificity tyrosine-phosphory...
    mbk-2 CELE_F49E11.1, F49E11.1
    502Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    A0A061ACN7A0A061ACN7_CAEEL
    Dual specificity tyrosine-phosphory...
    mbk-2 CELE_F49E11.1, F49E11.1
    514Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    A0A061AKR6A0A061AKR6_CAEEL
    Dual specificity tyrosine-phosphory...
    mbk-2 CELE_F49E11.1, F49E11.1
    779Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    A0A061ACN3A0A061ACN3_CAEEL
    Dual specificity tyrosine-phosphory...
    mbk-2 CELE_F49E11.1, F49E11.1
    523Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting. The information stored in this subsection is used to automatically construct alternative protein sequence(s) for display.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0531811 – 327Missing in isoform d. 1 PublicationAdd BLAST327
    Alternative sequenceiVSP_0531821 – 301Missing in isoform e. 1 PublicationAdd BLAST301
    Alternative sequenceiVSP_0536251 – 297Missing in isoform f. CuratedAdd BLAST297
    Alternative sequenceiVSP_0531831 – 294Missing in isoform a. 1 PublicationAdd BLAST294
    Alternative sequenceiVSP_0536261 – 282Missing in isoform h. CuratedAdd BLAST282
    Alternative sequenceiVSP_053627148 – 164Missing in isoform g. CuratedAdd BLAST17
    Alternative sequenceiVSP_053184295 – 310AAQAT…TSSSN → MTLFEPSTSGNRMGYR in isoform a. 1 PublicationAdd BLAST16
    Alternative sequenceiVSP_053628298 – 310ATGLP…TSSSN → MFAIPFHRFYSDE in isoform f. CuratedAdd BLAST13
    Alternative sequenceiVSP_053185302 – 310PNVGTSSSN → MYSSLFELR in isoform e. 1 Publication9
    Alternative sequenceiVSP_053186797 – 817KKTET…MRKKF → VCFIIF in isoform a, isoform d and isoform e. 1 PublicationAdd BLAST21
    Alternative sequenceiVSP_053187797 – 817Missing in isoform c. 2 PublicationsAdd BLAST21
    Alternative sequenceiVSP_053629797 – 802KKTETL → VCFIIF in isoform f. Curated6
    Alternative sequenceiVSP_053630803 – 817Missing in isoform f. CuratedAdd BLAST15

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AY090019 mRNA Translation: AAM09088.1
    Z70308 Genomic DNA Translation: CAA94352.1
    Z70308, Z81121, Z81146 Genomic DNA Translation: CAA94353.1
    Z70308, Z81121, Z81146 Genomic DNA Translation: CAB54254.2
    Z70308 Genomic DNA Translation: CAJ76942.1
    Z70308, Z81121 Genomic DNA Translation: CAJ80814.1
    Z70308, Z81121, Z81146 Genomic DNA Translation: CCG28133.1
    Z70308, Z81121 Genomic DNA Translation: CCG28134.1
    Z70308, Z81121 Genomic DNA Translation: CCM09396.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    T22440
    T22442

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_001023207.1, NM_001028036.2 [Q9XTF3-1]
    NP_001023208.1, NM_001028037.2 [Q9XTF3-3]
    NP_001040950.1, NM_001047485.3 [Q9XTF3-4]
    NP_001040951.1, NM_001047486.2 [Q9XTF3-5]
    NP_001255694.1, NM_001268765.1 [Q9XTF3-7]
    NP_001255695.1, NM_001268766.1 [Q9XTF3-6]
    NP_001263802.1, NM_001276873.1 [Q9XTF3-8]
    NP_502492.2, NM_070091.6 [Q9XTF3-2]

    UniGene gene-oriented nucleotide sequence clusters

    More...
    UniGenei
    Cel.39641
    Cel.7508

    Genome annotation databases

    Ensembl metazoan genome annotation project

    More...
    EnsemblMetazoai
    F49E11.1a; F49E11.1a; WBGene00003150 [Q9XTF3-2]
    F49E11.1b; F49E11.1b; WBGene00003150 [Q9XTF3-1]
    F49E11.1c; F49E11.1c; WBGene00003150 [Q9XTF3-3]
    F49E11.1d.1; F49E11.1d.1; WBGene00003150 [Q9XTF3-4]
    F49E11.1d.2; F49E11.1d.2; WBGene00003150 [Q9XTF3-4]
    F49E11.1d.3; F49E11.1d.3; WBGene00003150 [Q9XTF3-4]
    F49E11.1e; F49E11.1e; WBGene00003150 [Q9XTF3-5]
    F49E11.1f; F49E11.1f; WBGene00003150 [Q9XTF3-6]
    F49E11.1g; F49E11.1g; WBGene00003150 [Q9XTF3-7]
    F49E11.1h; F49E11.1h; WBGene00003150 [Q9XTF3-8]

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    178250

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    cel:CELE_F49E11.1

    UCSC genome browser

    More...
    UCSCi
    F49E11.1c c. elegans

    Keywords - Coding sequence diversityi

    Alternative splicing

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY090019 mRNA Translation: AAM09088.1
    Z70308 Genomic DNA Translation: CAA94352.1
    Z70308, Z81121, Z81146 Genomic DNA Translation: CAA94353.1
    Z70308, Z81121, Z81146 Genomic DNA Translation: CAB54254.2
    Z70308 Genomic DNA Translation: CAJ76942.1
    Z70308, Z81121 Genomic DNA Translation: CAJ80814.1
    Z70308, Z81121, Z81146 Genomic DNA Translation: CCG28133.1
    Z70308, Z81121 Genomic DNA Translation: CCG28134.1
    Z70308, Z81121 Genomic DNA Translation: CCM09396.1
    PIRiT22440
    T22442
    RefSeqiNP_001023207.1, NM_001028036.2 [Q9XTF3-1]
    NP_001023208.1, NM_001028037.2 [Q9XTF3-3]
    NP_001040950.1, NM_001047485.3 [Q9XTF3-4]
    NP_001040951.1, NM_001047486.2 [Q9XTF3-5]
    NP_001255694.1, NM_001268765.1 [Q9XTF3-7]
    NP_001255695.1, NM_001268766.1 [Q9XTF3-6]
    NP_001263802.1, NM_001276873.1 [Q9XTF3-8]
    NP_502492.2, NM_070091.6 [Q9XTF3-2]
    UniGeneiCel.39641
    Cel.7508

    3D structure databases

    ProteinModelPortaliQ9XTF3
    SMRiQ9XTF3
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi43342, 9 interactors
    ComplexPortaliCPX-3381 Egg-3/4/5 MBK-2 complex
    IntActiQ9XTF3, 11 interactors
    STRINGi6239.F49E11.1b

    PTM databases

    iPTMnetiQ9XTF3

    Proteomic databases

    EPDiQ9XTF3
    PaxDbiQ9XTF3
    PeptideAtlasiQ9XTF3
    PRIDEiQ9XTF3

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblMetazoaiF49E11.1a; F49E11.1a; WBGene00003150 [Q9XTF3-2]
    F49E11.1b; F49E11.1b; WBGene00003150 [Q9XTF3-1]
    F49E11.1c; F49E11.1c; WBGene00003150 [Q9XTF3-3]
    F49E11.1d.1; F49E11.1d.1; WBGene00003150 [Q9XTF3-4]
    F49E11.1d.2; F49E11.1d.2; WBGene00003150 [Q9XTF3-4]
    F49E11.1d.3; F49E11.1d.3; WBGene00003150 [Q9XTF3-4]
    F49E11.1e; F49E11.1e; WBGene00003150 [Q9XTF3-5]
    F49E11.1f; F49E11.1f; WBGene00003150 [Q9XTF3-6]
    F49E11.1g; F49E11.1g; WBGene00003150 [Q9XTF3-7]
    F49E11.1h; F49E11.1h; WBGene00003150 [Q9XTF3-8]
    GeneIDi178250
    KEGGicel:CELE_F49E11.1
    UCSCiF49E11.1c c. elegans

    Organism-specific databases

    Comparative Toxicogenomics Database

    More...
    CTDi
    178250
    WormBaseiF49E11.1a ; CE05897 ; WBGene00003150 ; mbk-2
    F49E11.1b ; CE19878 ; WBGene00003150 ; mbk-2
    F49E11.1c ; CE23751 ; WBGene00003150 ; mbk-2
    F49E11.1d ; CE39735 ; WBGene00003150 ; mbk-2
    F49E11.1e ; CE39938 ; WBGene00003150 ; mbk-2
    F49E11.1f ; CE47098 ; WBGene00003150 ; mbk-2
    F49E11.1g ; CE47427 ; WBGene00003150 ; mbk-2
    F49E11.1h ; CE47790 ; WBGene00003150 ; mbk-2

    Phylogenomic databases

    eggNOGiKOG0667 Eukaryota
    ENOG410XPET LUCA
    GeneTreeiENSGT00940000166363
    InParanoidiQ9XTF3
    KOiK18669
    OMAiNTASTHD
    OrthoDBiEOG091G0Q46
    PhylomeDBiQ9XTF3

    Enzyme and pathway databases

    ReactomeiR-CEL-6804756 Regulation of TP53 Activity through Phosphorylation
    SignaLinkiQ9XTF3

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:Q9XTF3

    Gene expression databases

    BgeeiWBGene00003150 Expressed in 5 organ(s), highest expression level in multi-cellular organism

    Family and domain databases

    InterProiView protein in InterPro
    IPR011009 Kinase-like_dom_sf
    IPR000719 Prot_kinase_dom
    IPR017441 Protein_kinase_ATP_BS
    IPR008271 Ser/Thr_kinase_AS
    PfamiView protein in Pfam
    PF00069 Pkinase, 1 hit
    SMARTiView protein in SMART
    SM00220 S_TKc, 1 hit
    SUPFAMiSSF56112 SSF56112, 1 hit
    PROSITEiView protein in PROSITE
    PS00107 PROTEIN_KINASE_ATP, 1 hit
    PS50011 PROTEIN_KINASE_DOM, 1 hit
    PS00108 PROTEIN_KINASE_ST, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMBK2_CAEEL
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9XTF3
    Secondary accession number(s): H9G2V8
    , H9G2V9, J7SF89, Q20604, Q27GP3, Q2EEP1, Q9TVF4
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 19, 2010
    Last sequence update: November 1, 1999
    Last modified: December 5, 2018
    This is version 148 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
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