UniProtKB - Q9XSZ4 (S4A4_RABIT)
Electrogenic sodium bicarbonate cotransporter 1
SLC4A4
Functioni
Electrogenic sodium/bicarbonate cotransporter with a Na+:HCO3- stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
By similarityMay have a higher activity than isoform 1.
By similarityActivity regulationi
GO - Molecular functioni
- inorganic anion exchanger activity Source: InterPro
- sodium:bicarbonate symporter activity Source: UniProtKB
GO - Biological processi
- sodium ion transport Source: UniProtKB
Keywordsi
Biological process | Ion transport, Sodium transport, Symport, Transport |
Ligand | Sodium |
Names & Taxonomyi
Protein namesi | Recommended name: Electrogenic sodium bicarbonate cotransporter 1Short name: Sodium bicarbonate cotransporter Alternative name(s): Na(+)/HCO3(-) cotransporter Solute carrier family 4 member 4 |
Gene namesi | Name:SLC4A4 Synonyms:NBC1, NBCE1 |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 466 | CytoplasmicBy similarityAdd BLAST | 466 | |
Transmembranei | 467 – 491 | Helical; Name=1By similarityAdd BLAST | 25 | |
Topological domaini | 492 – 501 | ExtracellularBy similarity | 10 | |
Transmembranei | 502 – 520 | Helical; Name=2By similarityAdd BLAST | 19 | |
Topological domaini | 521 | CytoplasmicBy similarity | 1 | |
Transmembranei | 522 – 542 | Discontinuously helical; Name=3By similarityAdd BLAST | 21 | |
Topological domaini | 543 – 550 | ExtracellularBy similarity | 8 | |
Transmembranei | 551 – 571 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 572 – 585 | CytoplasmicBy similarityAdd BLAST | 14 | |
Transmembranei | 586 – 609 | Helical; Name=5By similarityAdd BLAST | 24 | |
Topological domaini | 610 – 692 | ExtracellularBy similarityAdd BLAST | 83 | |
Transmembranei | 693 – 710 | Helical; Name=6By similarityAdd BLAST | 18 | |
Topological domaini | 711 – 725 | CytoplasmicBy similarityAdd BLAST | 15 | |
Transmembranei | 726 – 745 | Helical; Name=7By similarityAdd BLAST | 20 | |
Topological domaini | 746 – 779 | ExtracellularBy similarityAdd BLAST | 34 | |
Transmembranei | 780 – 807 | Helical; Name=8By similarityAdd BLAST | 28 | |
Topological domaini | 808 – 819 | CytoplasmicBy similarityAdd BLAST | 12 | |
Transmembranei | 820 – 836 | Helical; Name=9By similarityAdd BLAST | 17 | |
Topological domaini | 837 | ExtracellularBy similarity | 1 | |
Transmembranei | 838 – 855 | Discontinuously helical; Name=10By similarityAdd BLAST | 18 | |
Topological domaini | 856 – 877 | CytoplasmicBy similarityAdd BLAST | 22 | |
Transmembranei | 878 – 894 | Helical; Name=11By similarityAdd BLAST | 17 | |
Topological domaini | 895 – 901 | ExtracellularBy similarity | 7 | |
Transmembranei | 902 – 918 | Helical; Name=12By similarityAdd BLAST | 17 | |
Topological domaini | 919 – 960 | CytoplasmicBy similarityAdd BLAST | 42 | |
Intramembranei | 961 – 986 | Discontinuously helicalBy similarityAdd BLAST | 26 | |
Topological domaini | 987 – 1079 | CytoplasmicBy similarityAdd BLAST | 93 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079230 | 1 – 1079 | Electrogenic sodium bicarbonate cotransporter 1Add BLAST | 1079 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 30 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 49 | Phosphothreonine; by PKABy similarity | 1 | |
Modified residuei | 61 | PhosphoserineBy similarity | 1 | |
Modified residuei | 65 | PhosphoserineBy similarity | 1 | |
Modified residuei | 68 | PhosphoserineBy similarity | 1 | |
Modified residuei | 223 | PhosphoserineBy similarity | 1 | |
Modified residuei | 232 | PhosphoserineBy similarity | 1 | |
Modified residuei | 233 | PhosphoserineBy similarity | 1 | |
Modified residuei | 245 | PhosphoserineBy similarity | 1 | |
Modified residuei | 249 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 254 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 256 | PhosphoserineBy similarity | 1 | |
Modified residuei | 257 | PhosphoserineBy similarity | 1 | |
Modified residuei | 262 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 627 ↔ 629 | By similarity | ||
Glycosylationi | 641 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 661 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 674 ↔ 686 | By similarity | ||
Modified residuei | 1026 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 1029 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1034 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1044 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1069 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PRIDEi | Q9XSZ4 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Homodimer.
Interacts with CA2/carbonic anhydrase 2 and CA4/carbonic anhydrase 4 which may regulate transporter activity. Isoform 1 but not isoform 2 interacts with AHCYL1 (via PEST domain when phosphorylated); the interaction increases SLC4A4 isoform 1 activity.
Interacts with AHCYL2.
By similarityProtein-protein interaction databases
STRINGi | 9986.ENSOCUP00000005624 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 62 | Required for interaction with AHCYL1By similarityAdd BLAST | 62 | |
Regioni | 39 – 78 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 237 – 265 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 748 – 779 | Interaction with CA4By similarityAdd BLAST | 32 | |
Regioni | 1002 – 1004 | CA2-bindingBy similarity | 3 | |
Regioni | 1012 – 1079 | DisorderedSequence analysisAdd BLAST | 68 | |
Regioni | 1030 – 1033 | CA2-bindingBy similarity | 4 | |
Regioni | 1057 – 1059 | Required for basolateral targetingBy similarity | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 39 – 53 | Basic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 54 – 73 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 237 – 260 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 1012 – 1039 | Basic and acidic residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 1060 – 1079 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1172, Eukaryota |
InParanoidi | Q9XSZ4 |
OrthoDBi | 265068at2759 |
Family and domain databases
Gene3Di | 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR013769, Band3_cytoplasmic_dom IPR011531, HCO3_transpt-like_TM_dom IPR003020, HCO3_transpt_euk IPR003024, Na/HCO3_transpt IPR016152, PTrfase/Anion_transptr |
PANTHERi | PTHR11453, PTHR11453, 1 hit |
Pfami | View protein in Pfam PF07565, Band_3_cyto, 1 hit PF00955, HCO3_cotransp, 1 hit |
PRINTSi | PR01231, HCO3TRNSPORT PR01232, NAHCO3TRSPRT |
SUPFAMi | SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00834, ae, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEDEAVLDRG ASFLKHVCDE EEVEGHHTIY IGVHVPKSYR RRRRHKRKTG
60 70 80 90 100
HKEKREKERI SENYSDKSDV ENADESSSSI LKPLISPAAE RIRFILGEED
110 120 130 140 150
DSPAPPQLFT ELDELLAVDG QEMEWKETAR WIKFEEKVEQ GGERWSKPHV
160 170 180 190 200
ATLSLHSLFE LRTCMEKGSI MLDREATSLP QLVEMIVDHQ IETGLLKPDL
210 220 230 240 250
KDKVTYTLLR KHRHQTKKSN LRSLADIGKT VSSASRMFTS PDNGSPAMTH
260 270 280 290 300
RNLTSSSLND ISDKPEKDQL KNKFMKKLPR DAEASNVLVG EVDFLDTPFI
310 320 330 340 350
AFVRLQQAVM LGALTEVPVP TRFLFILLGP KGKAKSYHEI GRAIATLMSD
360 370 380 390 400
EVFHDIAYKA KDRHDLIAGI DEFLDEVIVL PPGEWDPAIR IEPPKSLPSS
410 420 430 440 450
DKRKNMYSGG ENVQMNGDTP HDGGHGGGGH ADCEELQRTG RFCGGLIKDL
460 470 480 490 500
KRKAPFFASD FYDALNIQSL SAILFIYLAT VTNAITFGGL LGDATDNMQG
510 520 530 540 550
VLESFLGTAV SGAIFCLFAG QPLTILSSTG PVLVFERLLF NFSKDHNFDY
560 570 580 590 600
LEFRLWIGLW SAFLCLILVA TDASFLVQYF TRFTEEGFSS LISFIFIYDA
610 620 630 640 650
FKKMIKLADY YPINSDFKVG YNTFFSCACV PPDPVNISIA NDTTPAPQEL
660 670 680 690 700
SAVSSTDMHQ NATFDWAFLS KKECLRYGGK LVGNNCNFVP DVTLMSFILF
710 720 730 740 750
LGTYTSSMAL KKFKTSRYFP TTARKLISDF AIILSILIFC VIDALVGVDT
760 770 780 790 800
PKLIVPSEFK PTSPNRGWFV PPFGGNPWWV YLAAAIPALL VTILIFMDQQ
810 820 830 840 850
ITAVIVNRKE HKLKKGAGYH LDLFWVAILM VVCSFMALPW YVAATVISIA
860 870 880 890 900
HIDSLKMETE TSAPGEQPKF LGVREQRVTG TLVFILTGLS VFMAPILKFI
910 920 930 940 950
PMPVLYGVFL YMGVASLNGV QFMDRLKLLL MPLKHQPDFI YLRHVPLRRV
960 970 980 990 1000
HLFTFLQVLC LALLWILKST VAAIIFPVMI LALVAVRKGM DYLFSQHDLS
1010 1020 1030 1040 1050
FLDDVIPEKD KKKKEDEKKK KKKKGSLDSD NDDSDCPYSE KVPSIKIPMD
1060 1070
IMEQQPFLSE SKPSDREKSS TFLERHTSC
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016715 | 1 – 44 | Missing in isoform 2. 1 PublicationAdd BLAST | 44 | |
Alternative sequenceiVSP_016716 | 45 – 85 | HKRKT…LKPLI → MSTENVEGKPSNLGERGRAR SYTFLRVVQPMFNLSIFTSA V in isoform 2. 1 PublicationAdd BLAST | 41 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF149418 mRNA Translation: AAD38154.1 AF119816 mRNA Translation: AAD18037.1 |
RefSeqi | NP_001075529.1, NM_001082060.1 [Q9XSZ4-1] |
Genome annotation databases
GeneIDi | 100008727 |
KEGGi | ocu:100008727 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF149418 mRNA Translation: AAD38154.1 AF119816 mRNA Translation: AAD18037.1 |
RefSeqi | NP_001075529.1, NM_001082060.1 [Q9XSZ4-1] |
3D structure databases
AlphaFoldDBi | Q9XSZ4 |
SMRi | Q9XSZ4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000005624 |
Proteomic databases
PRIDEi | Q9XSZ4 |
Genome annotation databases
GeneIDi | 100008727 |
KEGGi | ocu:100008727 |
Organism-specific databases
CTDi | 8671 |
Phylogenomic databases
eggNOGi | KOG1172, Eukaryota |
InParanoidi | Q9XSZ4 |
OrthoDBi | 265068at2759 |
Family and domain databases
Gene3Di | 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR013769, Band3_cytoplasmic_dom IPR011531, HCO3_transpt-like_TM_dom IPR003020, HCO3_transpt_euk IPR003024, Na/HCO3_transpt IPR016152, PTrfase/Anion_transptr |
PANTHERi | PTHR11453, PTHR11453, 1 hit |
Pfami | View protein in Pfam PF07565, Band_3_cyto, 1 hit PF00955, HCO3_cotransp, 1 hit |
PRINTSi | PR01231, HCO3TRNSPORT PR01232, NAHCO3TRSPRT |
SUPFAMi | SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00834, ae, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | S4A4_RABIT | |
Accessioni | Q9XSZ4Primary (citable) accession number: Q9XSZ4 Secondary accession number(s): O97915 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | November 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families