UniProtKB - Q9XSG3 (IDHC_BOVIN)
Protein
Isocitrate dehydrogenase [NADP] cytoplasmic
Gene
IDH1
Organism
Bos taurus (Bovine)
Status
Functioni
May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency.1 Publication
Catalytic activityi
- EC:1.1.1.42By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 77 | SubstrateBy similarity | 1 | |
Binding sitei | 82 | NADPBy similarity | 1 | |
Binding sitei | 109 | SubstrateBy similarity | 1 | |
Binding sitei | 132 | SubstrateBy similarity | 1 | |
Sitei | 139 | Critical for catalysisBy similarity | 1 | |
Binding sitei | 212 | Substrate; shared with neighboring subunitBy similarity | 1 | |
Sitei | 212 | Critical for catalysisBy similarity | 1 | |
Metal bindingi | 252 | Magnesium or manganese; shared with neighboring subunitBy similarity | 1 | |
Binding sitei | 260 | NADPBy similarity | 1 | |
Metal bindingi | 275 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 279 | Magnesium or manganeseBy similarity | 1 | |
Binding sitei | 328 | NADP; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 75 – 77 | NADPBy similarity | 3 | |
Nucleotide bindingi | 310 – 315 | NADPBy similarity | 6 |
GO - Molecular functioni
- isocitrate dehydrogenase (NADP+) activity Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- NAD binding Source: InterPro
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- glyoxylate cycle Source: UniProtKB-KW
- isocitrate metabolic process Source: UniProtKB
- NADP metabolic process Source: GO_Central
- response to oxidative stress Source: AgBase
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Manganese, Metal-binding, NADP |
Enzyme and pathway databases
SABIO-RKi | Q9XSG3 |
Names & Taxonomyi
Protein namesi | Recommended name: Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42By similarity)Short name: IDH Alternative name(s): Cytosolic NADP-isocitrate dehydrogenase IDP NADP(+)-specific ICDH Oxalosuccinate decarboxylase |
Gene namesi | Name:IDH1 Synonyms:ICDH |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol By similarity
Cytosol
- cytosol Source: AgBase
Mitochondrion
- mitochondrion Source: GO_Central
Peroxisome
- peroxisome Source: GO_Central
Other locations
- cytoplasm Source: AgBase
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000236188 | 2 – 414 | Isocitrate dehydrogenase [NADP] cytoplasmicAdd BLAST | 413 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 42 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 81 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 126 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 224 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 233 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 321 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 389 | PhosphoserineBy similarity | 1 | |
Modified residuei | 400 | N6-succinyllysineBy similarity | 1 |
Post-translational modificationi
Acetylation at Lys-374 dramatically reduces catalytic activity.By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q9XSG3 |
PeptideAtlasi | Q9XSG3 |
PRIDEi | Q9XSG3 |
Expressioni
Tissue specificityi
Expressed preferentially in corneal epithelium. Constitute approximately 13% of the total soluble bovine corneal epithelial proteins.1 Publication
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 158592, 1 interactor |
STRINGi | 9913.ENSBTAP00000027348 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 94 – 100 | Substrate bindingBy similarity | 7 |
Sequence similaritiesi
Belongs to the isocitrate and isopropylmalate dehydrogenases family.Curated
Phylogenomic databases
eggNOGi | KOG1526, Eukaryota |
HOGENOMi | CLU_023296_1_1_1 |
InParanoidi | Q9XSG3 |
OrthoDBi | 769322at2759 |
TreeFami | TF300428 |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR004790, Isocitrate_DH_NADP IPR024084, IsoPropMal-DH-like_dom |
PANTHERi | PTHR11822, PTHR11822, 1 hit |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
PIRSFi | PIRSF000108, IDH_NADP, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00127, nadp_idh_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9XSG3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQKIQGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD
60 70 80 90 100
ATNDQVTKDA AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG
160 170 180 190 200
KVEISYTPSD GSPKTVYLVH NFTESGGVAM GMYNQDKSIE DFAHSSFQMA
210 220 230 240 250
LSKNWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSEFE AQNIWYEHRL
260 270 280 290 300
IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG
310 320 330 340 350
KTVEAEAAHG TVTRHYRMYQ KGQETLTNPI ASIFAWTRGL AHRAKLDNNK
360 370 380 390 400
ELSFFAKALE EVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK
410
LGENLQLKLA QAKL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 326 | L → S in AAI03369 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF136009 mRNA Translation: AAD34457.1 BC103368 mRNA Translation: AAI03369.1 |
RefSeqi | NP_851355.2, NM_181012.3 XP_005202754.1, XM_005202697.1 |
Genome annotation databases
GeneIDi | 281235 |
KEGGi | bta:281235 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF136009 mRNA Translation: AAD34457.1 BC103368 mRNA Translation: AAI03369.1 |
RefSeqi | NP_851355.2, NM_181012.3 XP_005202754.1, XM_005202697.1 |
3D structure databases
SMRi | Q9XSG3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 158592, 1 interactor |
STRINGi | 9913.ENSBTAP00000027348 |
Proteomic databases
PaxDbi | Q9XSG3 |
PeptideAtlasi | Q9XSG3 |
PRIDEi | Q9XSG3 |
Genome annotation databases
GeneIDi | 281235 |
KEGGi | bta:281235 |
Organism-specific databases
CTDi | 3417 |
Phylogenomic databases
eggNOGi | KOG1526, Eukaryota |
HOGENOMi | CLU_023296_1_1_1 |
InParanoidi | Q9XSG3 |
OrthoDBi | 769322at2759 |
TreeFami | TF300428 |
Enzyme and pathway databases
SABIO-RKi | Q9XSG3 |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR004790, Isocitrate_DH_NADP IPR024084, IsoPropMal-DH-like_dom |
PANTHERi | PTHR11822, PTHR11822, 1 hit |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
PIRSFi | PIRSF000108, IDH_NADP, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00127, nadp_idh_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IDHC_BOVIN | |
Accessioni | Q9XSG3Primary (citable) accession number: Q9XSG3 Secondary accession number(s): Q3SYV2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2006 |
Last sequence update: | November 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families