UniProtKB - Q9XSC6 (KCRM_BOVIN)
Creatine kinase M-type
CKM
Functioni
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
By similarityCatalytic activityi
- EC:2.7.3.2PROSITE-ProRule annotationBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 191 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 236 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 292 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 335 | ATPPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 128 – 132 | ATPPROSITE-ProRule annotation | 5 | |
Nucleotide bindingi | 320 – 325 | ATPPROSITE-ProRule annotation | 6 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- creatine kinase activity Source: AgBase
- kinase activity Source: GO_Central
GO - Biological processi
- phosphocreatine biosynthetic process Source: AgBase
- response to heat Source: AgBase
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Creatine kinase M-type (EC:2.7.3.2By similarity)Alternative name(s): Creatine kinase M chain Creatine phosphokinase M-type Short name: CPK-M M-CK |
Gene namesi | Name:CKM |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000013921 |
VGNCi | VGNC:27385, CKM |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Extracellular region or secreted
- extracellular space Source: AgBase
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211973 | 1 – 381 | Creatine kinase M-typeAdd BLAST | 381 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 164 | PhosphoserineBy similarity | 1 | |
Modified residuei | 166 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 178 | PhosphoserineBy similarity | 1 | |
Modified residuei | 180 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 199 | PhosphoserineBy similarity | 1 | |
Modified residuei | 313 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 322 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 372 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9XSC6 |
PeptideAtlasi | Q9XSC6 |
PRIDEi | Q9XSC6 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000013921, Expressed in laryngeal cartilage and 80 other tissues |
ExpressionAtlasi | Q9XSC6, baseline |
Interactioni
Subunit structurei
Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000018492 |
Structurei
Secondary structure
3D structure databases
SMRi | Q9XSC6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9XSC6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 98 | Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 125 – 367 | Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 243 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3581, Eukaryota |
GeneTreei | ENSGT00950000182772 |
HOGENOMi | CLU_019868_4_2_1 |
InParanoidi | Q9XSC6 |
OMAi | STHAKFD |
OrthoDBi | 825025at2759 |
TreeFami | TF314214 |
Family and domain databases
Gene3Di | 1.10.135.10, 1 hit |
InterProi | View protein in InterPro IPR000749, ATP-guanido_PTrfase IPR022415, ATP-guanido_PTrfase_AS IPR022414, ATP-guanido_PTrfase_cat IPR022413, ATP-guanido_PTrfase_N IPR036802, ATP-guanido_PTrfase_N_sf IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11547, PTHR11547, 2 hits |
Pfami | View protein in Pfam PF00217, ATP-gua_Ptrans, 1 hit PF02807, ATP-gua_PtransN, 1 hit |
SUPFAMi | SSF48034, SSF48034, 1 hit SSF55931, SSF55931, 1 hit |
PROSITEi | View protein in PROSITE PS00112, PHOSPHAGEN_KINASE, 1 hit PS51510, PHOSPHAGEN_KINASE_C, 1 hit PS51509, PHOSPHAGEN_KINASE_N, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MPFGNTHNKH KLNFKAEEEY PDLSKHNNHM AKALTLEIYK KLRDKETPSG
60 70 80 90 100
FTLDDVIQTG VDNPGHPFIM TVGCVAGDEE SYTVFKDLFD PIIQDRHGGF
110 120 130 140 150
KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY ALPPHCSRGE
160 170 180 190 200
RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EQEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF
260 270 280 290 300
RRFCVGLQKI EEIFKKAGHP FMWNEHLGYV LTCPSNLGTG LRGGVHVKLA
310 320 330 340 350
HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV
360 370 380
QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ K
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1LX97 | A0A3Q1LX97_BOVIN | Creatine kinase | CKM | 388 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 376 | M → L in AAD30974 (Ref. 1) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 377 | I → M1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF120106 mRNA Translation: AAD30974.1 BT020785 mRNA Translation: AAX08802.1 BT021173 mRNA Translation: AAX31355.1 BC102907 mRNA Translation: AAI02908.1 |
RefSeqi | NP_777198.2, NM_174773.4 |
Genome annotation databases
Ensembli | ENSBTAT00000018492; ENSBTAP00000018492; ENSBTAG00000013921 |
GeneIDi | 286822 |
KEGGi | bta:286822 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF120106 mRNA Translation: AAD30974.1 BT020785 mRNA Translation: AAX08802.1 BT021173 mRNA Translation: AAX31355.1 BC102907 mRNA Translation: AAI02908.1 |
RefSeqi | NP_777198.2, NM_174773.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G0W | X-ray | 2.30 | A | 7-359 | [»] | |
SMRi | Q9XSC6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000018492 |
Protein family/group databases
Allergomei | 11907, Bos d CK |
Proteomic databases
PaxDbi | Q9XSC6 |
PeptideAtlasi | Q9XSC6 |
PRIDEi | Q9XSC6 |
Genome annotation databases
Ensembli | ENSBTAT00000018492; ENSBTAP00000018492; ENSBTAG00000013921 |
GeneIDi | 286822 |
KEGGi | bta:286822 |
Organism-specific databases
CTDi | 1158 |
VEuPathDBi | HostDB:ENSBTAG00000013921 |
VGNCi | VGNC:27385, CKM |
Phylogenomic databases
eggNOGi | KOG3581, Eukaryota |
GeneTreei | ENSGT00950000182772 |
HOGENOMi | CLU_019868_4_2_1 |
InParanoidi | Q9XSC6 |
OMAi | STHAKFD |
OrthoDBi | 825025at2759 |
TreeFami | TF314214 |
Miscellaneous databases
EvolutionaryTracei | Q9XSC6 |
Gene expression databases
Bgeei | ENSBTAG00000013921, Expressed in laryngeal cartilage and 80 other tissues |
ExpressionAtlasi | Q9XSC6, baseline |
Family and domain databases
Gene3Di | 1.10.135.10, 1 hit |
InterProi | View protein in InterPro IPR000749, ATP-guanido_PTrfase IPR022415, ATP-guanido_PTrfase_AS IPR022414, ATP-guanido_PTrfase_cat IPR022413, ATP-guanido_PTrfase_N IPR036802, ATP-guanido_PTrfase_N_sf IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11547, PTHR11547, 2 hits |
Pfami | View protein in Pfam PF00217, ATP-gua_Ptrans, 1 hit PF02807, ATP-gua_PtransN, 1 hit |
SUPFAMi | SSF48034, SSF48034, 1 hit SSF55931, SSF55931, 1 hit |
PROSITEi | View protein in PROSITE PS00112, PHOSPHAGEN_KINASE, 1 hit PS51510, PHOSPHAGEN_KINASE_C, 1 hit PS51509, PHOSPHAGEN_KINASE_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KCRM_BOVIN | |
Accessioni | Q9XSC6Primary (citable) accession number: Q9XSC6 Secondary accession number(s): Q5E9Y4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | June 27, 2006 | |
Last modified: | September 29, 2021 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families