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Protein

Tripeptidyl-peptidase 1

Gene

TPP1

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus (By similarity).By similarity

Catalytic activityi

Release of an N-terminal tripeptide from a polypeptide, but also has endopeptidase activity.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei272Charge relay systemBy similarity1
Active sitei276Charge relay systemBy similarity1
Active sitei475Charge relay systemBy similarity1
Metal bindingi517CalciumBy similarity1
Metal bindingi518Calcium; via carbonyl oxygenBy similarity1
Metal bindingi539Calcium; via carbonyl oxygenBy similarity1
Metal bindingi541Calcium; via carbonyl oxygenBy similarity1
Metal bindingi543CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
LigandCalcium, Metal-binding

Protein family/group databases

MEROPSiS53.003

Names & Taxonomyi

Protein namesi
Recommended name:
Tripeptidyl-peptidase 1 (EC:3.4.14.9)
Short name:
TPP-1
Alternative name(s):
Lysosomal pepstatin-insensitive protease
Short name:
LPIC
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase I
Short name:
TPP-I
Gene namesi
Name:TPP1
Synonyms:CLN2
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
PropeptideiPRO_000002737220 – 195Removed in mature formBy similarityAdd BLAST176
ChainiPRO_0000027373196 – 563Tripeptidyl-peptidase 1Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi111 ↔ 122By similarity
Glycosylationi210N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi222N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi286N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi313N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi365 ↔ 526By similarity
Glycosylationi443N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi522 ↔ 537By similarity

Post-translational modificationi

Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity (By similarity).By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9XSB8
PRIDEiQ9XSB8

Interactioni

Subunit structurei

Monomer. Interacts with CLN5.By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000009732

Structurei

3D structure databases

ProteinModelPortaliQ9XSB8
SMRiQ9XSB8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini199 – 563Peptidase S53Add BLAST365

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IICY Eukaryota
COG4934 LUCA
HOGENOMiHOG000171253
HOVERGENiHBG004449
InParanoidiQ9XSB8
KOiK01279

Family and domain databases

CDDicd04056 Peptidases_S53, 1 hit
cd11377 Pro-peptidase_S53, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR036852 Peptidase_S8/S53_dom_sf
IPR015366 S53_propep
IPR030400 Sedolisin_dom
PfamiView protein in Pfam
PF09286 Pro-kuma_activ, 1 hit
SMARTiView protein in SMART
SM00944 Pro-kuma_activ, 1 hit
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS51695 SEDOLISIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XSB8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLRTCLLGL LALCVASKCS YSPEPDQQRT LPPGWVSLGR VDSEEELSLT
60 70 80 90 100
FALRQQNVER LSKLVQAVSD PGSPHYGKYL TLEDVAELVR PSPLTFRTVQ
110 120 130 140 150
KWLSAAGARN CHSVTTQDFL TCWLSVRQAE LLLSGAEFHR YVGGPTEIHV
160 170 180 190 200
IRSLRPYQLP KALAPHVDFV GGLHRFPPTS SLRQRPEPQV SGTVGLHLGV
210 220 230 240 250
TPSVIRQRYN LTAQDVGSGT TNNSQACAQF LEQYFHASDL AEFMRLFGGN
260 270 280 290 300
FAHQASVARV VGQQGRGRAG IEASLDVEYL MSAGANISTW VYSSPGRHES
310 320 330 340 350
QEPFLQWLLL LSNESALPHV HTVSYGDDED SLSSAYIQRV NTEFMKAAAR
360 370 380 390 400
GLTLLFASGD SGAGCWSVSR RHQFRPSFPA SSPYVTTVGG TSFQNPFRVT
410 420 430 440 450
TEIVDYISGG GFSNVFPQPS YQEEAVVQFL SSSPHLPPSS YFNASGRAYP
460 470 480 490 500
DVAALSDGYW VVSNSVPIPW VSGTSASTPV FGGILSLINE HRLLSGLPPL
510 520 530 540 550
GFLNPRLYQQ RGAGLFDVTR GCHESCLNEE VQGQGFCSGP GWDPVTGWGT
560
PNFPALLKAL IKP
Length:563
Mass (Da):61,362
Last modified:November 1, 1999 - v1
Checksum:i21465A44C34934F4
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti427V → A2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114167 Genomic DNA Translation: AAD25043.1
DQ100344 Genomic DNA Translation: AAZ38727.1
RefSeqiNP_001013869.1, NM_001013847.1
UniGeneiCfa.18923

Genome annotation databases

GeneIDi485337
KEGGicfa:485337

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114167 Genomic DNA Translation: AAD25043.1
DQ100344 Genomic DNA Translation: AAZ38727.1
RefSeqiNP_001013869.1, NM_001013847.1
UniGeneiCfa.18923

3D structure databases

ProteinModelPortaliQ9XSB8
SMRiQ9XSB8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000009732

Protein family/group databases

MEROPSiS53.003

Proteomic databases

PaxDbiQ9XSB8
PRIDEiQ9XSB8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi485337
KEGGicfa:485337

Organism-specific databases

CTDi1200

Phylogenomic databases

eggNOGiENOG410IICY Eukaryota
COG4934 LUCA
HOGENOMiHOG000171253
HOVERGENiHBG004449
InParanoidiQ9XSB8
KOiK01279

Family and domain databases

CDDicd04056 Peptidases_S53, 1 hit
cd11377 Pro-peptidase_S53, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR036852 Peptidase_S8/S53_dom_sf
IPR015366 S53_propep
IPR030400 Sedolisin_dom
PfamiView protein in Pfam
PF09286 Pro-kuma_activ, 1 hit
SMARTiView protein in SMART
SM00944 Pro-kuma_activ, 1 hit
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS51695 SEDOLISIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPP1_CANLF
AccessioniPrimary (citable) accession number: Q9XSB8
Secondary accession number(s): Q45VT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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