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UniProtKB - Q9XJP3 (FIBER_BPSFV)
Protein
Tail spike protein
Gene
N/A
Organism
Shigella phage Sf6 (Shigella flexneri bacteriophage VI) (Bacteriophage SfVI)
Status
Functioni
Non-covalently bound to the neck of the phage capsid and mediating attachment of the viral particle to host cell-surface polysaccharide. It displays endorhamnosidase enzymatic activity, hydrolyzing the alpha-1,3-O-glycosidic linkage between rhamnose and galactose of the O-antigen polysaccharide.
2 Publications1 PublicationpH dependencei
Optimum pH is 7.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 248 | Substrate binding1 Publication | 1 | |
Sitei | 294 | Substrate binding1 Publication | 1 | |
Active sitei | 367 | Shared with dimeric partner1 Publication | 1 | |
Active sitei | 400 | Shared with dimeric partner1 Publication | 1 |
GO - Molecular functioni
- endo-1,3-alpha-L-rhamnosidase activity Source: UniProtKB
GO - Biological processi
- disruption by virus of host envelope lipopolysaccharide during virus entry Source: UniProtKB-KW
- entry into host Source: UniProtKB
- metabolic process Source: UniProtKB-KW
- virion attachment to host cell Source: UniProtKB
Keywordsi
Protein family/group databases
UniLectini | Q9XJP3 |
Names & Taxonomyi
Protein namesi | Recommended name: Tail spike proteinCuratedShort name: TSP Alternative name(s): Endo-1,3-alpha-L-rhamnosidaseCurated (EC:3.2.1.-3 Publications) EndorhamnosidaseCurated |
Organismi | Shigella phage Sf6 (Shigella flexneri bacteriophage VI) (Bacteriophage SfVI) |
Taxonomic identifieri | 10761 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Uroviricota › Caudoviricetes › Caudovirales › Podoviridae › Lederbergvirus |
Virus hosti | Shigella flexneri [TaxID: 623] |
Subcellular locationi
- Virion 1 Publication
Keywords - Cellular componenti
Viral tail fiber protein, Viral tail protein, VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 248 | D → N: 98.5% loss of enzymatic activity. 1 Publication | 1 | |
Mutagenesisi | 294 | E → Q: 87% loss of enzymatic activity. 1 Publication | 1 | |
Mutagenesisi | 367 | E → Q: Almost complete loss of enzymatic activity. 1 Publication | 1 | |
Mutagenesisi | 400 | D → N: Almost complete loss of enzymatic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000077758 | 1 – 623 | Tail spike proteinAdd BLAST | 623 |
Interactioni
Subunit structurei
Homotrimer.
2 PublicationsProtein-protein interaction databases
DIPi | DIP-29798N |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9XJP3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9XJP3 |
Family & Domainsi
Domaini
The active site involves residues located on two different subunits.1 Publication
Sequence similaritiesi
Belongs to the P22likevirus tail fiber protein family.Curated
Family and domain databases
Gene3Di | 2.160.20.10, 1 hit 2.170.14.10, 1 hit |
InterProi | View protein in InterPro IPR009093, P22_tailspike_N IPR036730, P22_tailspike_N_sf IPR024535, Pectate_lyase_SF_prot IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence |
Pfami | View protein in Pfam PF09008, Head_binding, 1 hit PF12708, Pectate_lyase_3, 1 hit |
SUPFAMi | SSF51126, SSF51126, 1 hit SSF51327, SSF51327, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9XJP3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDIITNVVI GMPSQLFTMA RSFKAVANGK IYIGKIDTDP VNPENQIQVY
60 70 80 90 100
VENEDGSHVP ASQPIVINAA GYPVYNGQIV KFVTEQGHSM AVYDAYGSQQ
110 120 130 140 150
FYFQNVLKYD PDQFGPDLIE QLAQSGKYSQ DNTKGDAMIG VKQPLPKAVL
160 170 180 190 200
RTQHDKNKEA ISILDFGVID DGVTDNYQAI QNAIDAVASL PSGGELFIPA
210 220 230 240 250
SNQAVGYIVG STLLIPGGVN IRGVGKASQL RAKSGLTGSV LRLSYDSDTI
260 270 280 290 300
GRYLRNIRVT GNNTCNGIDT NITAEDSVIR QVYGWVFDNV MVNEVETAYL
310 320 330 340 350
MQGLWHSKFI ACQAGTCRVG LHFLGQCVSV SVSSCHFSRG NYSADESFGI
360 370 380 390 400
RIQPQTYAWS SEAVRSEAII LDSETMCIGF KNAVYVHDCL DLHMEQLDLD
410 420 430 440 450
YCGSTGVVIE NVNGGFSFSN SWIAADADGT EQFTGIYFRT PTSTQSHKIV
460 470 480 490 500
SGVHINTANK NTAANNQSIA IEQSAIFVFV SGCTLTGDEW AVNIVDINEC
510 520 530 540 550
VSFDKCIFNK PLRYLRSGGV SVTDCYLAGI TEVQKPEGRY NTYRGCSGVP
560 570 580 590 600
SVNGIINVPV AVGATSGSAA IPNPGNLTYR VRSLFGDPAS SGDKVSVSGV
610 620
TINVTRPSPV GVALPSMVEY LAI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF128887 Genomic DNA Translation: AAD33394.2 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF128887 Genomic DNA Translation: AAD33394.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VBE | X-ray | 1.98 | A | 110-623 | [»] | |
2VBK | X-ray | 1.25 | A | 110-623 | [»] | |
2VBM | X-ray | 2.00 | A | 110-623 | [»] | |
4URR | X-ray | 1.95 | A/B/C/D/E/F | 110-623 | [»] | |
SMRi | Q9XJP3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-29798N |
Protein family/group databases
UniLectini | Q9XJP3 |
Miscellaneous databases
EvolutionaryTracei | Q9XJP3 |
Family and domain databases
Gene3Di | 2.160.20.10, 1 hit 2.170.14.10, 1 hit |
InterProi | View protein in InterPro IPR009093, P22_tailspike_N IPR036730, P22_tailspike_N_sf IPR024535, Pectate_lyase_SF_prot IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence |
Pfami | View protein in Pfam PF09008, Head_binding, 1 hit PF12708, Pectate_lyase_3, 1 hit |
SUPFAMi | SSF51126, SSF51126, 1 hit SSF51327, SSF51327, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FIBER_BPSFV | |
Accessioni | Q9XJP3Primary (citable) accession number: Q9XJP3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 30, 2003 | |
Last modified: | September 29, 2021 | |
This is version 82 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families