UniProtKB - Q9XJ32 (VTSS1_SOLTU)
Protein
Vetispiradiene synthase 1
Gene
PVS1
Organism
Solanum tuberosum (Potato)
Status
Functioni
Sesquiterpene synthase that catalyzes the formation of vetispiradiene from trans,trans-farnesyl diphosphate. The initial internal cyclization produces the monocyclic intermediate germacrene A.
Catalytic activityi
- EC:4.2.3.211 Publication
Cofactori
Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity
: terpenoid biosynthesis Pathwayi
This protein is involved in the pathway terpenoid biosynthesis, which is part of Secondary metabolite biosynthesis.View all proteins of this organism that are known to be involved in the pathway terpenoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 309 | Magnesium 1By similarity | 1 | |
Metal bindingi | 309 | Magnesium 2By similarity | 1 | |
Metal bindingi | 313 | Magnesium 1By similarity | 1 | |
Metal bindingi | 313 | Magnesium 2By similarity | 1 | |
Metal bindingi | 452 | Magnesium 3By similarity | 1 | |
Metal bindingi | 456 | Magnesium 3By similarity | 1 | |
Metal bindingi | 460 | Magnesium 3By similarity | 1 |
GO - Molecular functioni
- magnesium ion binding Source: InterPro
- vetispiradiene synthase activity Source: UniProtKB-EC
GO - Biological processi
- terpenoid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Lyase |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00213 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PVS1 |
Organismi | Solanum tuberosum (Potato) |
Taxonomic identifieri | 4113 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000398187 | 1 – 556 | Vetispiradiene synthase 1Add BLAST | 556 |
Expressioni
Inductioni
By infection with P.infestans.1 Publication
Gene expression databases
ExpressionAtlasi | Q9XJ32, differential |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 309 – 313 | DDXXD motif | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 224 – 227 | Poly-Glu | 4 |
Domaini
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QUCN, Eukaryota |
InParanoidi | Q9XJ32 |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 1 hit SSF48576, SSF48576, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9XJ32-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTPAAVVMSN YGEEEIVRPI ADFSPSLWGD RFHSFSLDNQ IAGKYAQEIE
60 70 80 90 100
TLKEQSRIIL SASSRRTLAE KLDLIDIVER LGIAYHFEKQ IDDMLDQFYK
110 120 130 140 150
ADPNFEAHEY NDLQTLSVQF RLLRQHGYNI SPKLFIRFQD AKGKFKESLC
160 170 180 190 200
NDIKGLLNLY EASHVRTHGE DILEEALAFS TAHLESAAPH LKSPLSKQVT
210 220 230 240 250
HALEQSLHKS IPRVETRYFI SIYEEEEQKN DVLLQFAKLD FNLLQMLHKQ
260 270 280 290 300
ELSEVSRWWK DLDFVTTLPY ARDRAVECYF WTMGVYAEPQ YSQARVMLAK
310 320 330 340 350
TIAMISIVDD TFDAYGIVKE LEIYTDAIQR WDISQIDRLP DYMKISYKAL
360 370 380 390 400
LDLYNDYEME LSKDGRSDVV HYAKERMKEI VRNYFVEAKW FIEGYMPPVS
410 420 430 440 450
EYLSNALATS TYYLLTTTSY LGMKSANKQD FEWLAKNPKI LEANVTLCRV
460 470 480 490 500
IDDIATYEVE KGRGQIATGI ECYMRDYGVS TEKAMEKFQE MAETAWKDVN
510 520 530 540 550
EGILRPTPVS TEILTRILNL ARIIDVTYKH NQDGYTHPEK VLKPHIIALL
VDSIEI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB022598 mRNA Translation: BAA82092.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB022598 mRNA Translation: BAA82092.1 |
3D structure databases
SMRi | Q9XJ32 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4113.PGSC0003DMT400046973 |
Phylogenomic databases
eggNOGi | ENOG502QUCN, Eukaryota |
InParanoidi | Q9XJ32 |
Enzyme and pathway databases
UniPathwayi | UPA00213 |
Gene expression databases
ExpressionAtlasi | Q9XJ32, differential |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 1 hit SSF48576, SSF48576, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VTSS1_SOLTU | |
Accessioni | Q9XJ32Primary (citable) accession number: Q9XJ32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 5, 2010 |
Last sequence update: | November 1, 1999 | |
Last modified: | August 12, 2020 | |
This is version 79 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families