ALA3

Functionⁱ

Involved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Required for secretory processes during plant development. Requires an interaction with an ALIS protein for activity. Has activity with phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine, but not with lysolipid (PubMed:18344284, PubMed:19566596, PubMed:20053675). Modifies endomembranes in multiple cell types, enabling structural changes, or signaling functions that are critical for normal development and adaptation to varied growth environments (PubMed:23667493).4 Publications

Miscellaneous

The intracellular targeting signals and lipid specificity determinants reside in the catalytic ALA subunit.1 Publication

Catalytic activityⁱ

ATP + H₂O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	413	4-aspartylphosphate intermediateBy similarity	1
Metal bindingⁱ	845	MagnesiumBy similarity	1
Metal bindingⁱ	849	MagnesiumBy similarity	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
magnesium ion binding Source: InterPro
phospholipid-translocating ATPase activity Source: GO_Central
phospholipid transporter activity
Source: TAIR
Inferred from direct assayⁱ
- 18344284

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

Golgi vesicle budding
Source: TAIR
Inferred from mutant phenotypeⁱ
- 18344284
root development
Source: TAIR
Inferred from mutant phenotypeⁱ
- 18344284
shoot system development
Source: TAIR
Inferred from mutant phenotypeⁱ
- 18344284

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	ARA:AT1G59820-MONOMER
BRENDAⁱ	3.6.99.B1 399
Reactomeⁱ	R-ATH-6798695 Neutrophil degranulation R-ATH-936837 Ion transport by P-type ATPases

Protein family/group databases

TCDBⁱ	3.A.3.8.6 the p-type atpase (p-atpase) superfamily

TCDBⁱ

3.A.3.8.6 the p-type atpase (p-atpase) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Phospholipid-transporting ATPase 31 Publication (EC:3.6.3.11 Publication) Short name: AtALA31 Publication Alternative name(s): Aminophospholipid ATPase 31 Publication Aminophospholipid flippase 31 Publication Protein IRREGULAR TRICHOME BRANCH 21 Publication
Gene namesⁱ	Name:ALA31 Publication Synonyms:ITB21 Publication Ordered Locus Names:At1g59820Imported ORF Names:F23H11.14Imported
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 1

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT1G59820
TAIRⁱ	locus:2025961 AT1G59820

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 67	CytoplasmicSequence analysisAdd BLAST	67
Transmembraneⁱ	68 – 89	HelicalSequence analysisAdd BLAST	22
Topological domainⁱ	90 – 93	ExtracellularSequence analysis	4
Transmembraneⁱ	94 – 116	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	117 – 297	CytoplasmicSequence analysisAdd BLAST	181
Transmembraneⁱ	298 – 319	HelicalSequence analysisAdd BLAST	22
Topological domainⁱ	320 – 346	ExtracellularSequence analysisAdd BLAST	27
Transmembraneⁱ	347 – 364	HelicalSequence analysisAdd BLAST	18
Topological domainⁱ	365 – 900	CytoplasmicSequence analysisAdd BLAST	536
Transmembraneⁱ	901 – 920	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	921 – 934	ExtracellularSequence analysisAdd BLAST	14
Transmembraneⁱ	935 – 954	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	955 – 984	CytoplasmicSequence analysisAdd BLAST	30
Transmembraneⁱ	985 – 1006	HelicalSequence analysisAdd BLAST	22
Topological domainⁱ	1007 – 1013	ExtracellularSequence analysis	7
Transmembraneⁱ	1014 – 1036	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	1037 – 1042	CytoplasmicSequence analysis	6
Transmembraneⁱ	1043 – 1063	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	1064 – 1076	ExtracellularSequence analysisAdd BLAST	13
Transmembraneⁱ	1077 – 1087	HelicalSequence analysisAdd BLAST	11
Topological domainⁱ	1088 – 1213	CytoplasmicSequence analysisAdd BLAST	126

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Impaired growth of roots and shoots (PubMed:18344284, PubMed:23667493). Roots devoided of the characteristic trans-Golgi proliferation of slime vesicles containing polysaccharides and enzymes for secretion (PubMed:18344284). Aberrant trichome expansion, reduced primary root growth and longer root hairs (PubMed:19566596). Impaired pollen growth (PubMed:19566596, PubMed:23667493). Impaired ovule development (PubMed:23667493). Reduced adaptability to temperature stresses (PubMed:23667493).3 Publications

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	413	D → A: Loss of internalization of phospholipids. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000046387	1 – 1213	Phospholipid-transporting ATPase 3Add BLAST		1213

Proteomic databases

PaxDbⁱ	Q9XIE6
PRIDEⁱ	Q9XIE6

PTM databases

iPTMnetⁱ	Q9XIE6

iPTMnetⁱ

Q9XIE6

Expressionⁱ

Tissue specificityⁱ

Expressed in petals and sepals, but not in reproductive tissues. In siliques, detected in the upper part of the seed pod and in the area between the seed pod and the stem, but not in developing seeds. Strong expression in vascular shoot tissues and in stomatal guard cells of young rosettes leaves. In roots, expressed in cells surrounding the xylem and in central and peripheral columella cells. Detected in developing and mature trichomes, roots, pollen and growing pollen tubes.2 Publications

Gene expression databases

ExpressionAtlasⁱ	Q9XIE6 baseline and differential
Genevisibleⁱ	Q9XIE6 AT

Interactionⁱ

Subunit structureⁱ

Associates with ALIS1 to form a stable and active complex. Interacts with ALIS3 and ALIS5 in a heterologous system.2 Publications

Protein-protein interaction databases

BioGridⁱ	27500, 1 interactor
STRINGⁱ	3702.AT1G59820.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9XIE6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410ITKD Eukaryota ENOG410XPYK LUCA
HOGENOMⁱ	HOG000202528
InParanoidⁱ	Q9XIE6
KEGG Orthology (KO) More...KOⁱ	K14802
OMAⁱ	RMRKQRG
Database of Orthologous Groups More...OrthoDBⁱ	EOG093600FE
PhylomeDBⁱ	Q9XIE6

Family and domain databases

Gene3Dⁱ	3.40.1110.10, 1 hit 3.40.50.1000, 1 hit
InterProⁱ	View protein in InterPro IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR036412 HAD-like_sf IPR023214 HAD_sf IPR006539 P-type_ATPase_IV IPR032631 P-type_ATPase_N IPR001757 P_typ_ATPase IPR032630 P_typ_ATPase_c
PANTHERⁱ	PTHR24092 PTHR24092, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16212 PhoLip_ATPase_C, 1 hit PF16209 PhoLip_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 3 hits SSF81653 SSF81653, 2 hits SSF81660 SSF81660, 2 hits SSF81665 SSF81665, 3 hits
TIGRFAMsⁱ	TIGR01652 ATPase-Plipid, 1 hit TIGR01494 ATPase_P-type, 1 hit
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9XIE6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVRSGSFSVD SSATHQRTPS RTVTLGHIQP QAPTYRTVYC NDRESNQPVR 
        60         70         80         90        100
FKGNSISTTK YNVFTFLPKG LFEQFRRIAN IYFLGISCLS MTPISPVSPI 
       110        120        130        140        150
TNVAPLSMVL LVSLIKEAFE DWKRFQNDMS INNSTVEILQ DQQWVSIPWR 
       160        170        180        190        200
KLQVGDIVKI KKDGFFPADI LFMSSTNSDG ICYVETANLD GETNLKIRKA 
       210        220        230        240        250
LERTWDYLVP EKAYEFKGEI QCEQPNNSLY TFTGNLVVQK QTLPLSPDQL 
       260        270        280        290        300
LLRGCSLRNT EYIVGAVVFT GHETKVMMNA MNAPSKRSTL EKKLDKLIIT 
       310        320        330        340        350
IFCVLVTMCL IGAIGCSIVT DREDKYLGLH NSDWEYRNGL MIGFFTFFTL 
       360        370        380        390        400
VTLFSSIIPI SLYVSIEMIK FIQSTQFINR DLNMYHAETN TPASARTSNL 
       410        420        430        440        450
NEELGQVEYI FSDKTGTLTR NLMEFFKCSI GGVSYGCGVT EIEKGIAQRH 
       460        470        480        490        500
GLKVQEEQRS TGAIREKGFN FDDPRLMRGA WRNEPNPDLC KELFRCLAIC 
       510        520        530        540        550
HTVLPEGDES PEKIVYQAAS PDEAALVTAA KNFGFFFYRR TPTMVYVRES 
       560        570        580        590        600
HVEKMGKIQD VAYEILNVLE FNSTRKRQSV VCRFPDGRLV LYCKGADNVI 
       610        620        630        640        650
FERLANGMDD VRKVTREHLE HFGSSGLRTL CLAYKDLNPE TYDSWNEKFI 
       660        670        680        690        700
QAKSALRDRE KKLDEVAELI EKDLILIGST AIEDKLQEGV PTCIETLSRA 
       710        720        730        740        750
GIKIWVLTGD KMETAINIAY ACNLINNEMK QFVISSETDA IREAEERGDQ 
       760        770        780        790        800
VEIARVIKEE VKRELKKSLE EAQHSLHTVA GPKLSLVIDG KCLMYALDPS 
       810        820        830        840        850
LRVMLLSLSL NCTSVVCCRV SPLQKAQVTS LVRKGAQKIT LSIGDGANDV 
       860        870        880        890        900
SMIQAAHVGI GISGMEGMQA VMASDFAIAQ FRFLTDLLLV HGRWSYLRIC 
       910        920        930        940        950
KVVMYFFYKN LTFTLTQFWF TFRTGFSGQR FYDDWFQSLF NVVFTALPVI 
       960        970        980        990       1000
VLGLFEKDVS ASLSKRYPEL YREGIRNSFF KWRVVAVWAT SAVYQSLVCY 
      1010       1020       1030       1040       1050
LFVTTSSFGA VNSSGKVFGL WDVSTMVFTC LVIAVNVRIL LMSNSITRWH 
      1060       1070       1080       1090       1100
YITVGGSILA WLVFAFVYCG IMTPHDRNEN VYFVIYVLMS TFYFYFTLLL 
      1110       1120       1130       1140       1150
VPIVSLLGDF IFQGVERWFF PYDYQIVQEI HRHESDASKA DQLEVENELT 
      1160       1170       1180       1190       1200
PQEARSYAIS QLPRELSKHT GFAFDSPGYE SFFASQLGIY APQKAWDVAR 
      1210 
RASMRSRPKV PKK

Length:1,213

Mass (Da):137,753

Last modified:February 28, 2003 - v2

Checksum:ⁱ938642DEFDA28B66

GO

Sequence cautionⁱ

The sequence AAD39325 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AC007258 Genomic DNA Translation: AAD39325.1 Sequence problems. CP002684 Genomic DNA Translation: AEE33621.1 AY091777 mRNA Translation: AAM10325.1
PIRⁱ	C96622
NCBI Reference Sequences More...RefSeqⁱ	NP_176191.1, NM_104675.3
UniGeneⁱ	At.36777

Genome annotation databases

EnsemblPlantsⁱ	AT1G59820.1; AT1G59820.1; AT1G59820
GeneIDⁱ	842275
Grameneⁱ	AT1G59820.1; AT1G59820.1; AT1G59820
KEGGⁱ	ath:AT1G59820

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q9XIE6	Phospholipid-transporting ATPase		ARATH		1213
UPI00053AF9CB		CAMSA		1224
UPI000859D024		RAPSA		1221
UPI0004F1732A		BRACM		1218
UPI0006AAFCCE		BRANA		1218
+40

Protein	Similar proteins		Species	Score	Length
Q9XIE6	Phospholipid-transporting ATPase		ARATH		1213
UPI00053AF9CB		CAMSA		1224
UPI000859D024		RAPSA		1221
UPI0004F1732A		BRACM		1218
UPI0006AAFCCE		BRANA		1218
+257

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AC007258 Genomic DNA Translation: AAD39325.1 Sequence problems. CP002684 Genomic DNA Translation: AEE33621.1 AY091777 mRNA Translation: AAM10325.1
PIRⁱ	C96622
NCBI Reference Sequences More...RefSeqⁱ	NP_176191.1, NM_104675.3
UniGeneⁱ	At.36777

3D structure databases

ProteinModelPortalⁱ	Q9XIE6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	27500, 1 interactor
STRINGⁱ	3702.AT1G59820.1

Protein family/group databases

TCDBⁱ	3.A.3.8.6 the p-type atpase (p-atpase) superfamily

TCDBⁱ

3.A.3.8.6 the p-type atpase (p-atpase) superfamily

PTM databases

iPTMnetⁱ	Q9XIE6

iPTMnetⁱ

Q9XIE6

Proteomic databases

PaxDbⁱ	Q9XIE6
PRIDEⁱ	Q9XIE6

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT1G59820.1; AT1G59820.1; AT1G59820
GeneIDⁱ	842275
Grameneⁱ	AT1G59820.1; AT1G59820.1; AT1G59820
KEGGⁱ	ath:AT1G59820

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT1G59820
TAIRⁱ	locus:2025961 AT1G59820

Phylogenomic databases

eggNOGⁱ	ENOG410ITKD Eukaryota ENOG410XPYK LUCA
HOGENOMⁱ	HOG000202528
InParanoidⁱ	Q9XIE6
KEGG Orthology (KO) More...KOⁱ	K14802
OMAⁱ	RMRKQRG
Database of Orthologous Groups More...OrthoDBⁱ	EOG093600FE
PhylomeDBⁱ	Q9XIE6

Enzyme and pathway databases

BioCycⁱ	ARA:AT1G59820-MONOMER
BRENDAⁱ	3.6.99.B1 399
Reactomeⁱ	R-ATH-6798695 Neutrophil degranulation R-ATH-936837 Ion transport by P-type ATPases

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9XIE6

PROⁱ

PR:Q9XIE6

Gene expression databases

ExpressionAtlasⁱ	Q9XIE6 baseline and differential
Genevisibleⁱ	Q9XIE6 AT

Family and domain databases

Gene3Dⁱ	3.40.1110.10, 1 hit 3.40.50.1000, 1 hit
InterProⁱ	View protein in InterPro IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR036412 HAD-like_sf IPR023214 HAD_sf IPR006539 P-type_ATPase_IV IPR032631 P-type_ATPase_N IPR001757 P_typ_ATPase IPR032630 P_typ_ATPase_c
PANTHERⁱ	PTHR24092 PTHR24092, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16212 PhoLip_ATPase_C, 1 hit PF16209 PhoLip_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 3 hits SSF81653 SSF81653, 2 hits SSF81660 SSF81660, 2 hits SSF81665 SSF81665, 3 hits
TIGRFAMsⁱ	TIGR01652 ATPase-Plipid, 1 hit TIGR01494 ATPase_P-type, 1 hit
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ALA3_ARATH
Accessionⁱ	Q9XIE6Primary (citable) accession number: Q9XIE6 Secondary accession number(s): Q8RWQ3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
	Last sequence update:	February 28, 2003
	Last modified:	April 25, 2018
	This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

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UniProtKB - Q9XIE6 (ALA3_ARATH)

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Phospholipid-transporting ATPase 3

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Golgi apparatus

Endoplasmic reticulum

Endosome

Golgi apparatus

Plasma Membrane

Other locations

Topology

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ