UniProtKB - Q9XIE6 (ALA3_ARATH)
Phospholipid-transporting ATPase 3
ALA3
Functioni
Involved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Required for secretory processes during plant development. Requires an interaction with an ALIS protein for activity. Has activity with phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine, but not with lysolipid (PubMed:18344284, PubMed:19566596, PubMed:20053675).
Modifies endomembranes in multiple cell types, enabling structural changes, or signaling functions that are critical for normal development and adaptation to varied growth environments (PubMed:23667493).
Required for the trafficking and endocytic recycling of ABCG36/PEN3 between the trans-Golgi network and the plasma membrane; thus promoting ABCG36/PEN3 recruitment to the host-pathogen interface upon infection by powdery mildews (e.g. Blumeria graminis) and bacteria (e.g. Pseudomonas syringae), or upon the detection of pathogen-associated molecular patterns (PAMPs) (e.g. flg22 and chitin) (PubMed:28434950).
5 PublicationsMiscellaneous
Catalytic activityi
- ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).1 Publication EC:7.6.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 413 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 845 | MagnesiumBy similarity | 1 | |
Metal bindingi | 849 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- aminophospholipid flippase activity Source: TAIR
- ATPase-coupled intramembrane lipid transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- magnesium ion binding Source: InterPro
GO - Biological processi
- defense response to bacterium Source: UniProtKB
- defense response to fungus Source: UniProtKB
- Golgi to plasma membrane transport Source: UniProtKB
- Golgi vesicle budding Source: TAIR
- phospholipid translocation Source: GO_Central
- protein localization Source: UniProtKB
- protein localization involved in auxin polar transport Source: TAIR
- protein transport Source: UniProtKB-KW
- response to molecule of bacterial origin Source: UniProtKB
- response to molecule of fungal origin Source: UniProtKB
- root development Source: TAIR
- shoot system development Source: TAIR
- vesicle-mediated transport Source: TAIR
Keywordsi
Molecular function | Translocase |
Biological process | Plant defense, Protein transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT1G59820-MONOMER |
BRENDAi | 7.6.2.1, 399 |
Protein family/group databases
TCDBi | 3.A.3.8.6, the p-type atpase (p-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipid-transporting ATPase 31 Publication (EC:7.6.2.11 Publication)Short name: AtALA31 Publication Alternative name(s): Aminophospholipid ATPase 31 Publication Aminophospholipid flippase 31 Publication Protein ABERRANT LOCALIZATION OF PEN3 31 Publication Protein IRREGULAR TRICHOME BRANCH 21 Publication |
Gene namesi | Name:ALA31 Publication Synonyms:ALP31 Publication, ITB21 Publication Ordered Locus Names:At1g59820Imported ORF Names:F23H11.14Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G59820 |
TAIRi | locus:2025961, AT1G59820 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Golgi apparatus
- Golgi apparatus membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Note: Requires the presence of an ALIS protein to exit the endoplasmic reticulum to the Golgi.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Endosome
- endosome Source: TAIR
- recycling endosome Source: TAIR
Golgi apparatus
- Golgi apparatus Source: TAIR
- Golgi membrane Source: UniProtKB-SubCell
- trans-Golgi network Source: TAIR
Nucleus
- nucleus Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
- pollen tube tip Source: TAIR
- vesicle Source: TAIR
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 67 | CytoplasmicSequence analysisAdd BLAST | 67 | |
Transmembranei | 68 – 89 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 90 – 93 | ExtracellularSequence analysis | 4 | |
Transmembranei | 94 – 116 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 117 – 297 | CytoplasmicSequence analysisAdd BLAST | 181 | |
Transmembranei | 298 – 319 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 320 – 346 | ExtracellularSequence analysisAdd BLAST | 27 | |
Transmembranei | 347 – 364 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 365 – 900 | CytoplasmicSequence analysisAdd BLAST | 536 | |
Transmembranei | 901 – 920 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 921 – 934 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 935 – 954 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 955 – 984 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 985 – 1006 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 1007 – 1013 | ExtracellularSequence analysis | 7 | |
Transmembranei | 1014 – 1036 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 1037 – 1042 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 1043 – 1063 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1064 – 1076 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 1077 – 1087 | HelicalSequence analysisAdd BLAST | 11 | |
Topological domaini | 1088 – 1213 | CytoplasmicSequence analysisAdd BLAST | 126 |
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 413 | D → A: Loss of internalization of phospholipids. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046387 | 1 – 1213 | Phospholipid-transporting ATPase 3Add BLAST | 1213 |
Proteomic databases
PaxDbi | Q9XIE6 |
PRIDEi | Q9XIE6 |
ProteomicsDBi | 244925 |
PTM databases
iPTMneti | Q9XIE6 |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | Q9XIE6, baseline and differential |
Genevisiblei | Q9XIE6, AT |
Interactioni
Subunit structurei
Associates with ALIS1 to form a stable and active complex.
Interacts with ALIS3 and ALIS5 in a heterologous system.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 27500, 1 interactor |
STRINGi | 3702.AT1G59820.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 23 | DisorderedSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0206, Eukaryota |
HOGENOMi | CLU_000846_5_2_1 |
InParanoidi | Q9XIE6 |
OMAi | MGNQRSY |
OrthoDBi | 587717at2759 |
PhylomeDBi | Q9XIE6 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVRSGSFSVD SSATHQRTPS RTVTLGHIQP QAPTYRTVYC NDRESNQPVR
60 70 80 90 100
FKGNSISTTK YNVFTFLPKG LFEQFRRIAN IYFLGISCLS MTPISPVSPI
110 120 130 140 150
TNVAPLSMVL LVSLIKEAFE DWKRFQNDMS INNSTVEILQ DQQWVSIPWR
160 170 180 190 200
KLQVGDIVKI KKDGFFPADI LFMSSTNSDG ICYVETANLD GETNLKIRKA
210 220 230 240 250
LERTWDYLVP EKAYEFKGEI QCEQPNNSLY TFTGNLVVQK QTLPLSPDQL
260 270 280 290 300
LLRGCSLRNT EYIVGAVVFT GHETKVMMNA MNAPSKRSTL EKKLDKLIIT
310 320 330 340 350
IFCVLVTMCL IGAIGCSIVT DREDKYLGLH NSDWEYRNGL MIGFFTFFTL
360 370 380 390 400
VTLFSSIIPI SLYVSIEMIK FIQSTQFINR DLNMYHAETN TPASARTSNL
410 420 430 440 450
NEELGQVEYI FSDKTGTLTR NLMEFFKCSI GGVSYGCGVT EIEKGIAQRH
460 470 480 490 500
GLKVQEEQRS TGAIREKGFN FDDPRLMRGA WRNEPNPDLC KELFRCLAIC
510 520 530 540 550
HTVLPEGDES PEKIVYQAAS PDEAALVTAA KNFGFFFYRR TPTMVYVRES
560 570 580 590 600
HVEKMGKIQD VAYEILNVLE FNSTRKRQSV VCRFPDGRLV LYCKGADNVI
610 620 630 640 650
FERLANGMDD VRKVTREHLE HFGSSGLRTL CLAYKDLNPE TYDSWNEKFI
660 670 680 690 700
QAKSALRDRE KKLDEVAELI EKDLILIGST AIEDKLQEGV PTCIETLSRA
710 720 730 740 750
GIKIWVLTGD KMETAINIAY ACNLINNEMK QFVISSETDA IREAEERGDQ
760 770 780 790 800
VEIARVIKEE VKRELKKSLE EAQHSLHTVA GPKLSLVIDG KCLMYALDPS
810 820 830 840 850
LRVMLLSLSL NCTSVVCCRV SPLQKAQVTS LVRKGAQKIT LSIGDGANDV
860 870 880 890 900
SMIQAAHVGI GISGMEGMQA VMASDFAIAQ FRFLTDLLLV HGRWSYLRIC
910 920 930 940 950
KVVMYFFYKN LTFTLTQFWF TFRTGFSGQR FYDDWFQSLF NVVFTALPVI
960 970 980 990 1000
VLGLFEKDVS ASLSKRYPEL YREGIRNSFF KWRVVAVWAT SAVYQSLVCY
1010 1020 1030 1040 1050
LFVTTSSFGA VNSSGKVFGL WDVSTMVFTC LVIAVNVRIL LMSNSITRWH
1060 1070 1080 1090 1100
YITVGGSILA WLVFAFVYCG IMTPHDRNEN VYFVIYVLMS TFYFYFTLLL
1110 1120 1130 1140 1150
VPIVSLLGDF IFQGVERWFF PYDYQIVQEI HRHESDASKA DQLEVENELT
1160 1170 1180 1190 1200
PQEARSYAIS QLPRELSKHT GFAFDSPGYE SFFASQLGIY APQKAWDVAR
1210
RASMRSRPKV PKK
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007258 Genomic DNA Translation: AAD39325.1 Sequence problems. CP002684 Genomic DNA Translation: AEE33621.1 AY091777 mRNA Translation: AAM10325.1 |
PIRi | C96622 |
RefSeqi | NP_176191.1, NM_104675.3 |
Genome annotation databases
EnsemblPlantsi | AT1G59820.1; AT1G59820.1; AT1G59820 |
GeneIDi | 842275 |
Gramenei | AT1G59820.1; AT1G59820.1; AT1G59820 |
KEGGi | ath:AT1G59820 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007258 Genomic DNA Translation: AAD39325.1 Sequence problems. CP002684 Genomic DNA Translation: AEE33621.1 AY091777 mRNA Translation: AAM10325.1 |
PIRi | C96622 |
RefSeqi | NP_176191.1, NM_104675.3 |
3D structure databases
AlphaFoldDBi | Q9XIE6 |
SMRi | Q9XIE6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 27500, 1 interactor |
STRINGi | 3702.AT1G59820.1 |
Protein family/group databases
TCDBi | 3.A.3.8.6, the p-type atpase (p-atpase) superfamily |
PTM databases
iPTMneti | Q9XIE6 |
Proteomic databases
PaxDbi | Q9XIE6 |
PRIDEi | Q9XIE6 |
ProteomicsDBi | 244925 |
Genome annotation databases
EnsemblPlantsi | AT1G59820.1; AT1G59820.1; AT1G59820 |
GeneIDi | 842275 |
Gramenei | AT1G59820.1; AT1G59820.1; AT1G59820 |
KEGGi | ath:AT1G59820 |
Organism-specific databases
Araporti | AT1G59820 |
TAIRi | locus:2025961, AT1G59820 |
Phylogenomic databases
eggNOGi | KOG0206, Eukaryota |
HOGENOMi | CLU_000846_5_2_1 |
InParanoidi | Q9XIE6 |
OMAi | MGNQRSY |
OrthoDBi | 587717at2759 |
PhylomeDBi | Q9XIE6 |
Enzyme and pathway databases
BioCyci | ARA:AT1G59820-MONOMER |
BRENDAi | 7.6.2.1, 399 |
Miscellaneous databases
PROi | PR:Q9XIE6 |
Gene expression databases
ExpressionAtlasi | Q9XIE6, baseline and differential |
Genevisiblei | Q9XIE6, AT |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALA3_ARATH | |
Accessioni | Q9XIE6Primary (citable) accession number: Q9XIE6 Secondary accession number(s): Q8RWQ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | February 28, 2003 | |
Last modified: | May 25, 2022 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families