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UniProtKB - Q9XES5 (DFRA_MALDO)
Protein
Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase
Gene
DFR
Organism
Malus domestica (Apple) (Pyrus malus)
Status
Functioni
Bifunctional enzyme involved in flavonoid metabolism. May use dihydroquercetin, dihydrokaempferol, eriodictyol, garbanzol (5-deoxydihydrokaempferol), dihydrofisetin (5-deoxydihydroquercetin), naringenin to a low extent (10%), but not 5-deoxynaringenin or butin (5-deoxyeriodictyol) as substrate.
1 PublicationCatalytic activityi
Kineticsi
- KM=3.9 µM for eriodictyol1 Publication
- KM=34.0 µM for dihydrokaempferol1 Publication
- KM=26.0 µM for dihydroquercetin1 Publication
- Vmax=4.6 nmol/sec/g enzyme toward eriodictyol1 Publication
- Vmax=186.0 nmol/sec/g enzyme toward dihydrokaempferol1 Publication
- Vmax=91.0 nmol/sec/g enzyme toward dihydroquercetin1 Publication
pH dependencei
Optimum pH is 5.75 with dihydroquercetin as substrate.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 44 | NADPBy similarity | 1 | |
Binding sitei | 163 | NADPBy similarity | 1 |
GO - Molecular functioni
- dihydrokaempferol 4-reductase activity Source: UniProtKB-EC
- flavanone 4-reductase activity Source: UniProtKB-EC
GO - Biological processi
- flavonoid biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Flavonoid biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.234, 3164 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DFR |
Organismi | Malus domestica (Apple) (Pyrus malus) |
Taxonomic identifieri | 3750 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Rosales › Rosaceae › Amygdaloideae › Maleae › Malus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000367056 | 1 – 348 | Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductaseAdd BLAST | 348 |
Expressioni
Developmental stagei
Expressed during fruit ripening.1 Publication
Family & Domainsi
Sequence similaritiesi
Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.Curated
Phylogenomic databases
OrthoDBi | 992332at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR001509, Epimerase_deHydtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF01370, Epimerase, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9XES5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSESESVCV TGASGFIGSW LVMRLLEHGY TVRATVRDPT NQKKVKHLLD
60 70 80 90 100
LPKAETHLTL WKADLADEGS FDEAIQGCSG VFHVATPMDF ESKDPENEVI
110 120 130 140 150
KPTINGLLDI LKACQKAKTV RKLVFTSSAG TVNVEEHQKP VYDESNWSDV
160 170 180 190 200
EFCRSVKMTG WMYFVSKTLA EQAAWKYAKE NNIDFITIIP TLVIGPFLMP
210 220 230 240 250
SMPPSLITGL SPILRNESHY GIIKQGQYVH LDDLCLSHIY LYEHPKAEGR
260 270 280 290 300
YICSSHDATI HELVKMLREK YPEYNIPTKF KGIDDNLEPV HFSSKKLREI
310 320 330 340
GFEFKYSLED MFVGAVDACR AKGLIPIPIP AEKTEAAEES NLVDVKVG
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 347 | V → A in strain: cv. M9. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF117268 mRNA Translation: AAD26204.1 AY227729 mRNA Translation: AAO39817.1 AY227728 mRNA Translation: AAO39816.1 AB074488 mRNA Translation: BAB92999.1 |
RefSeqi | NP_001280868.1, NM_001293939.1 |
Genome annotation databases
EnsemblPlantsi | mRNA:MD15G0020300; mRNA:MD15G0020300; MD15G0020300 |
GeneIDi | 103450464 |
Gramenei | mRNA:MD15G0020300; mRNA:MD15G0020300; MD15G0020300 |
KEGGi | mdm:103450464 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF117268 mRNA Translation: AAD26204.1 AY227729 mRNA Translation: AAO39817.1 AY227728 mRNA Translation: AAO39816.1 AB074488 mRNA Translation: BAB92999.1 |
RefSeqi | NP_001280868.1, NM_001293939.1 |
3D structure databases
SMRi | Q9XES5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3750.XP_008388041.1 |
Genome annotation databases
EnsemblPlantsi | mRNA:MD15G0020300; mRNA:MD15G0020300; MD15G0020300 |
GeneIDi | 103450464 |
Gramenei | mRNA:MD15G0020300; mRNA:MD15G0020300; MD15G0020300 |
KEGGi | mdm:103450464 |
Phylogenomic databases
OrthoDBi | 992332at2759 |
Enzyme and pathway databases
BRENDAi | 1.1.1.234, 3164 |
Family and domain databases
InterProi | View protein in InterPro IPR001509, Epimerase_deHydtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF01370, Epimerase, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DFRA_MALDO | |
Accessioni | Q9XES5Primary (citable) accession number: Q9XES5 Secondary accession number(s): Q84KP1, Q8L5N3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 75 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |