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Protein

Calcium-transporting ATPase 4, endoplasmic reticulum-type

Gene

ECA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi336Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi337Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi339Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi341Calcium 2By similarity1
Active sitei3834-aspartylphosphate intermediateBy similarity1
Metal bindingi731MagnesiumBy similarity1
Metal bindingi735MagnesiumBy similarity1
Metal bindingi797Calcium 1By similarity1
Metal bindingi800Calcium 1By similarity1
Metal bindingi825Calcium 2By similarity1
Metal bindingi828Calcium 1By similarity1
Metal bindingi829Calcium 1By similarity1
Metal bindingi829Calcium 2By similarity1
Metal bindingi961Calcium 1By similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G07670-MONOMER
BRENDAi3.6.3.8 399

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 4, endoplasmic reticulum-type (EC:3.6.3.8)
Gene namesi
Name:ECA4
Ordered Locus Names:At1g07670
ORF Names:F24B9.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G07670
TAIRilocus:2026555 AT1G07670

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 70CytoplasmicSequence analysisAdd BLAST70
Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
Topological domaini92 – 115LumenalSequence analysisAdd BLAST24
Transmembranei116 – 135HelicalSequence analysisAdd BLAST20
Topological domaini136 – 278CytoplasmicSequence analysisAdd BLAST143
Transmembranei279 – 298HelicalSequence analysisAdd BLAST20
Topological domaini299 – 327LumenalSequence analysisAdd BLAST29
Transmembranei328 – 345HelicalSequence analysisAdd BLAST18
Topological domaini346 – 786CytoplasmicSequence analysisAdd BLAST441
Transmembranei787 – 806HelicalSequence analysisAdd BLAST20
Topological domaini807 – 816LumenalSequence analysis10
Transmembranei817 – 837HelicalSequence analysisAdd BLAST21
Topological domaini838 – 857CytoplasmicSequence analysisAdd BLAST20
Transmembranei858 – 880HelicalSequence analysisAdd BLAST23
Topological domaini881 – 950LumenalSequence analysisAdd BLAST70
Transmembranei951 – 970HelicalSequence analysisAdd BLAST20
Topological domaini971 – 983CytoplasmicSequence analysisAdd BLAST13
Transmembranei984 – 1002HelicalSequence analysisAdd BLAST19
Topological domaini1003 – 1017LumenalSequence analysisAdd BLAST15
Transmembranei1018 – 1038HelicalSequence analysisAdd BLAST21
Topological domaini1039 – 1061CytoplasmicSequence analysisAdd BLAST23

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464081 – 1061Calcium-transporting ATPase 4, endoplasmic reticulum-typeAdd BLAST1061

Proteomic databases

PaxDbiQ9XES1
PRIDEiQ9XES1

PTM databases

SwissPalmiQ9XES1

Expressioni

Inductioni

Induced by cadmium.1 Publication

Gene expression databases

ExpressionAtlasiQ9XES1 baseline and differential
GenevisibleiQ9XES1 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G07670.1

Structurei

3D structure databases

ProteinModelPortaliQ9XES1
SMRiQ9XES1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202 Eukaryota
COG0474 LUCA
HOGENOMiHOG000265621
InParanoidiQ9XES1
KOiK01537
OMAiAKIRDWG
OrthoDBiEOG093600UE
PhylomeDBiQ9XES1

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9XES1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKGGEDCGN KQTNSSELVK SDTFPAWGKD VSECEEKFGV SREKGLSTDE
60 70 80 90 100
VLKRHQIYGL NELEKPEGTS IFKLILEQFN DTLVRILLAA AVISFVLAFF
110 120 130 140 150
DGDEGGEMGI TAFVEPLVIF LILIVNAIVG IWQETNAEKA LEALKEIQSQ
160 170 180 190 200
QATVMRDGTK VSSLPAKELV PGDIVELRVG DKVPADMRVV ALISSTLRVE
210 220 230 240 250
QGSLTGESEA VSKTTKHVDE NADIQGKKCM VFAGTTVVNG NCICLVTDTG
260 270 280 290 300
MNTEIGRVHS QIQEAAQHEE DTPLKKKLNE FGEVLTMIIG LICALVWLIN
310 320 330 340 350
VKYFLSWEYV DGWPRNFKFS FEKCTYYFEI AVALAVAAIP EGLPAVITTC
360 370 380 390 400
LALGTRKMAQ KNALVRKLPS VETLGCTTVI CSDKTGTLTT NQMAVSKLVA
410 420 430 440 450
MGSRIGTLRS FNVEGTSFDP RDGKIEDWPT GRMDANLQMI AKIAAICNDA
460 470 480 490 500
NVEKSDQQFV SRGMPTEAAL KVLVEKMGFP EGLNEASSDG NVLRCCRLWS
510 520 530 540 550
ELEQRIATLE FDRDRKSMGV MVDSSSGKKL LLVKGAVENV LERSTHIQLL
560 570 580 590 600
DGSTRELDQY SRDLILQSLH DMSLSALRCL GFAYSDVPSD FATYDGSEDH
610 620 630 640 650
PAHQQLLNPS NYSSIESNLV FVGFVGLRDP PRKEVRQAIA DCRTAGIRVM
660 670 680 690 700
VITGDNKSTA EAICREIGVF EADEDISSRS LTGKEFMDVK DQKNHLRQTG
710 720 730 740 750
GLLFSRAEPK HKQEIVRLLK EDGEVVAMTG DGVNDAPALK LADIGVAMGI
760 770 780 790 800
SGTEVAKEAS DLVLADDNFS TIVAAVGEGR SIYNNMKAFI RYMISSNIGE
810 820 830 840 850
VASIFLTAAL GIPEGMIPVQ LLWVNLVTDG PPATALGFNP PDKDIMKKPP
860 870 880 890 900
RRSDDSLITA WILFRYMVIG LYVGVATVGV FIIWYTHNSF MGIDLSQDGH
910 920 930 940 950
SLVSYSQLAH WGQCSSWEGF KVSPFTAGSQ TFSFDSNPCD YFQQGKIKAS
960 970 980 990 1000
TLSLSVLVAI EMFNSLNALS EDGSLVTMPP WVNPWLLLAM AVSFGLHFVI
1010 1020 1030 1040 1050
LYVPFLAQVF GIVPLSLNEW LLVLAVSLPV ILIDEVLKFV GRCTSGYRYS
1060
PRTPSAKQKE E
Length:1,061
Mass (Da):116,180
Last modified:January 11, 2001 - v2
Checksum:i55B6126E5D539822
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti314P → H in AAD29957 (Ref. 3) Curated1
Sequence conflicti430T → M in AAD29957 (Ref. 3) Curated1
Sequence conflicti439M → S in AAD29957 (Ref. 3) Curated1
Sequence conflicti659T → I in AAD29957 (Ref. 3) Curated1
Sequence conflicti859T → P in AAD29957 (Ref. 3) Curated1
Sequence conflicti1046G → V in AAD29957 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007583 Genomic DNA Translation: AAF75088.1
CP002684 Genomic DNA Translation: AEE28160.1
CP002684 Genomic DNA Translation: ANM58604.1
AF117125 mRNA Translation: AAD29957.1
PIRiF86211
T52332
RefSeqiNP_001321027.1, NM_001331716.1
NP_172246.3, NM_100640.4
UniGeneiAt.26214

Genome annotation databases

EnsemblPlantsiAT1G07670.1; AT1G07670.1; AT1G07670
AT1G07670.2; AT1G07670.2; AT1G07670
GeneIDi837280
GrameneiAT1G07670.1; AT1G07670.1; AT1G07670
AT1G07670.2; AT1G07670.2; AT1G07670
KEGGiath:AT1G07670

Similar proteinsi

Entry informationi

Entry nameiECA4_ARATH
AccessioniPrimary (citable) accession number: Q9XES1
Secondary accession number(s): Q9LQP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: May 23, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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